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ES2170861T3 - Procedimiento de ensayo de extension de adn polimerasa. - Google Patents

Procedimiento de ensayo de extension de adn polimerasa.

Info

Publication number
ES2170861T3
ES2170861T3 ES96920611T ES96920611T ES2170861T3 ES 2170861 T3 ES2170861 T3 ES 2170861T3 ES 96920611 T ES96920611 T ES 96920611T ES 96920611 T ES96920611 T ES 96920611T ES 2170861 T3 ES2170861 T3 ES 2170861T3
Authority
ES
Spain
Prior art keywords
product
assay
oligonucleotide
dna polymerase
test procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96920611T
Other languages
English (en)
Inventor
James L Cole
Lawrence C Kuo
David B Olsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of ES2170861T3 publication Critical patent/ES2170861T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE HA DESARROLLADO UN ANALISIS DE ENZIMAS QUE ACTUAN SOBRE SUSTRATOS PARA PRODUCIR UN PRODUCTO OLIGONUCLEOTIDO DE CADENA SENCILLA QUE IMPLICA LA EXTENSION DEL ADN CATALIZADA POR POLIMERASA DEL PRODUCTO DE DIVISION DEL OLIGONUCLEOTIDO UTILIZANDO NUCLEOTIDOS MARCADOS Y UNA PLANTILLA TEMPLETE DE ADN QUE CONTIENE UNA REGION 3'' COMPLEMENTARIA DEL PRODUCTO OLIGONUCLEOTIDO UNIDO A LA REGION 5'' FORMADA POR RESIDUOS REPETIDOS DE OLIGONUCLEOTIDOS. EL ANALISIS DE EXTENSION DE LA ADN POLIMERASA NO CONLLEVA SEPARACION ELECTROFORETICA EN GEL Y ES SENSIBLE PARA LA DETECCION SELECTIVA DE GRANDES VOLUMENES DE INHIBIDORES POTENCIALES. OTRAS CARACTERISTICAS FUNDAMENTALES DEL ENSAYO SON QUE CONTROLA LA REACCION DE DIVISION DEL SUSTRATO SOLO EN LA POSICION CORRECTA DE LA SECUENCIA, CON LO QUE DISCRIMINA ENTRE PRODUCTOS DE DIVISION INESPECIFICOS, Y QUE TIENE UNA SENSIBILIDAD SUFICIENTE PARA DETECTAR 200 ATOMOLES DE PRODUCTO.
ES96920611T 1995-06-07 1996-06-03 Procedimiento de ensayo de extension de adn polimerasa. Expired - Lifetime ES2170861T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48776095A 1995-06-07 1995-06-07

Publications (1)

Publication Number Publication Date
ES2170861T3 true ES2170861T3 (es) 2002-08-16

Family

ID=23937013

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96920611T Expired - Lifetime ES2170861T3 (es) 1995-06-07 1996-06-03 Procedimiento de ensayo de extension de adn polimerasa.

Country Status (7)

Country Link
EP (1) EP0833945B1 (es)
JP (1) JPH11506607A (es)
AT (1) ATE212676T1 (es)
CA (1) CA2222688A1 (es)
DE (1) DE69618957T2 (es)
ES (1) ES2170861T3 (es)
WO (1) WO1996040994A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5660989A (en) * 1995-06-07 1997-08-26 Merck & Co., Inc. DNA polymerase extension assay for influenza virus endonuclease
IL122147A0 (en) * 1997-11-10 1998-04-05 Technion Res & Dev Foundation Method for labeling polynucleotides
ATE414169T1 (de) 1998-03-05 2008-11-15 Johnson & Johnson Res Pty Ltd Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5660989A (en) * 1995-06-07 1997-08-26 Merck & Co., Inc. DNA polymerase extension assay for influenza virus endonuclease

Also Published As

Publication number Publication date
EP0833945B1 (en) 2002-01-30
DE69618957D1 (de) 2002-03-14
EP0833945A4 (en) 1999-05-19
JPH11506607A (ja) 1999-06-15
EP0833945A1 (en) 1998-04-08
WO1996040994A1 (en) 1996-12-19
CA2222688A1 (en) 1996-12-19
ATE212676T1 (de) 2002-02-15
DE69618957T2 (de) 2002-08-14

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