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HUP0303377A2 - Eljárás nukleinsav-molekulák kimutatására - Google Patents

Eljárás nukleinsav-molekulák kimutatására

Info

Publication number
HUP0303377A2
HUP0303377A2 HU0303377A HUP0303377A HUP0303377A2 HU P0303377 A2 HUP0303377 A2 HU P0303377A2 HU 0303377 A HU0303377 A HU 0303377A HU P0303377 A HUP0303377 A HU P0303377A HU P0303377 A2 HUP0303377 A2 HU P0303377A2
Authority
HU
Hungary
Prior art keywords
nucleic acid
acid molecules
detection
subject
polymerase chain
Prior art date
Application number
HU0303377A
Other languages
English (en)
Inventor
Martin Hubert
Hans Kroath
Axel Mündlein
Wolfgang Schmidt
Original Assignee
Austrian Research Centers Gmbh-Arc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Austrian Research Centers Gmbh-Arc filed Critical Austrian Research Centers Gmbh-Arc
Publication of HUP0303377A2 publication Critical patent/HUP0303377A2/hu
Publication of HUP0303377A3 publication Critical patent/HUP0303377A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A találmány tárgyát eljárás képezi egy mintában található legalábbkét, egymástól eltérő nukleinsav-molekula egyidejű kimutatására,melynek első lépéseként többszörös (<multiplex<) polimeráz-láncreakciót hajtanak végre, második lépésként pedig szilárd hordozómikromátrixon rögzített próbák alkalmazásával hibridizálási reakciótvégeznek, majd a hibridizálódott PCR-termékeket kimutatják, és azokat,adott esetben, mennyiségi meghatározásnak vetik alá. A találmánytárgyát képezi továbbá egy szilárd mikrohordozó és egy próbakészlettöbbszörös polimeráz láncreakcióval kapott termékekhibridizáltatására, továbbá egy reagenskészlet adott mintábantalálható legalább két, egymástól eltérő nukleinsav-molekula egyidejűkimutatására. Ó
HU0303377A 2001-03-02 2002-03-01 Method for the detection of nucleic acid molecules HUP0303377A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0033701A AT410444B (de) 2001-03-02 2001-03-02 Verfahren zur detektion von nukleinsäuremolekülen
PCT/AT2002/000060 WO2002070736A2 (de) 2001-03-02 2002-03-01 Verfahren zur detektion von nukleinsäuremolekülen

Publications (2)

Publication Number Publication Date
HUP0303377A2 true HUP0303377A2 (hu) 2004-01-28
HUP0303377A3 HUP0303377A3 (en) 2005-12-28

Family

ID=3672000

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0303377A HUP0303377A3 (en) 2001-03-02 2002-03-01 Method for the detection of nucleic acid molecules

Country Status (16)

Country Link
US (2) US20050196756A1 (hu)
EP (1) EP1366195B1 (hu)
JP (2) JP2004520838A (hu)
KR (1) KR100892184B1 (hu)
AT (1) AT410444B (hu)
AU (1) AU2002238274B2 (hu)
CA (1) CA2439531A1 (hu)
CZ (1) CZ20032665A3 (hu)
DE (1) DE50204680D1 (hu)
DK (1) DK1366195T3 (hu)
ES (1) ES2252427T3 (hu)
HU (1) HUP0303377A3 (hu)
NO (1) NO20033884L (hu)
PL (1) PL365022A1 (hu)
SK (1) SK287793B6 (hu)
WO (1) WO2002070736A2 (hu)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003144153A (ja) * 2001-11-09 2003-05-20 Gifu Univ 遺伝子検出方法、遺伝子検出用プライマー、ｄｎａマイクロアレイ及び遺伝子検出用キット
KR100619189B1 (ko) * 2004-10-08 2006-08-31 굿젠 주식회사 성교전파성질환 원인균 탐지용 프로브 및 이를 이용한성교전파성질환 원인균 유전자형 분석용 ｄｎａ 칩,분석키트 및 유전형 분석방법
EP1674583A1 (en) * 2004-12-23 2006-06-28 Eppendorf Array Technologies SA Method and kit for the detection of a large number of genes related to antibiotic resistance in microorganisms
US20070059714A1 (en) * 2005-09-12 2007-03-15 Birgit Strommenger Detection of presence and antibiotic susceptibility of enterococci
AT504194B1 (de) * 2006-09-07 2008-07-15 Oesterr Rotes Kreuz Bakteriennachweis
EP2270203A1 (en) 2009-06-29 2011-01-05 AIT Austrian Institute of Technology GmbH Oligonucleotide hybridization method
CN104508492B (zh) * 2012-05-25 2018-07-27 北卡罗来纳-查佩尔山大学 微流体装置、用于试剂的固体支持体和相关方法
CN105121656A (zh) * 2012-11-15 2015-12-02 以色列分子检测有限公司 Pcr反应混合物及使用其的方法
CN108025304B (zh) 2015-07-22 2022-01-21 北卡罗来纳-查佩尔山大学 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法
JP2019537706A (ja) * 2016-10-07 2019-12-26 ベーリンガー インゲルハイム フェトメディカ ゲーエムベーハーＢｏｅｈｒｉｎｇｅｒ Ｉｎｇｅｌｈｅｉｍ Ｖｅｔｍｅｄｉｃａ ＧｍｂＨ サンプルを検査するための方法及び分析システム
JP6987133B2 (ja) * 2016-10-07 2021-12-22 ベーリンガー インゲルハイム フェトメディカ ゲーエムベーハーBoehringer Ingelheim Vetmedica GmbH サンプルを検査するための方法及び分析システム
CN110114145B (zh) 2016-10-07 2022-08-09 勃林格殷格翰维特梅迪卡有限公司 用于检测样品的分析系统及方法
CN112154190A (zh) 2018-05-18 2020-12-29 北卡罗来纳-查佩尔山大学 用于改善基材的表面性质的组合物、装置和方法

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JPH02501027A (ja) * 1986-10-30 1990-04-12 シナージェン　バイオロジカルズ，インコーポレイティド 組換えｄｎａ法により生産されるヒト膵臓分泌トリプシンインヒビターおよびその生産方法
JPH04501204A (ja) * 1988-08-11 1992-03-05 サイオス　ノバ　インコーポレイテッド 異種タンパク発現を安定させる方法およびそれに使用するベクター
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5491225A (en) * 1991-12-19 1996-02-13 Hoffmann-La Roche Inc. PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays
US5837832A (en) * 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US6045996A (en) * 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US6063566A (en) * 1994-05-13 2000-05-16 The Scripps Research Institute Catalytic RNA molecules
US6150093A (en) * 1994-08-18 2000-11-21 The Trustees Of Columbia University In The City Of New York Unique associated Kaposi's sarcoma virus sequences and uses thereof
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US5702895A (en) * 1995-01-19 1997-12-30 Wakunaga Seiyaku Kabushiki Kaisha Method and kit for detecting methicillin-resistant Staphylococcus aureus
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US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US5627054A (en) * 1996-04-05 1997-05-06 The United States Of America As Represented By The Secretary Of The Army Competitor primer asymmetric polymerase chain reaction
GB9902970D0 (en) * 1999-02-11 1999-03-31 Zeneca Ltd Novel matrix
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CN1117161C (zh) * 1999-09-24 2003-08-06 东南大学 高密度基因芯片的制作方法

Also Published As

Publication number Publication date
JP2004520838A (ja) 2004-07-15
NO20033884L (no) 2003-10-28
US20050196756A1 (en) 2005-09-08
AU2002238274B2 (en) 2007-06-28
CA2439531A1 (en) 2002-09-12
NO20033884D0 (no) 2003-09-02
EP1366195A2 (de) 2003-12-03
JP2011101652A (ja) 2011-05-26
CZ20032665A3 (cs) 2005-02-16
SK287793B6 (sk) 2011-10-04
ES2252427T3 (es) 2006-05-16
WO2002070736A2 (de) 2002-09-12
DE50204680D1 (de) 2005-12-01
AT410444B (de) 2003-04-25
DK1366195T3 (da) 2006-03-13
HUP0303377A3 (en) 2005-12-28
EP1366195B1 (de) 2005-10-26
SK10832003A3 (sk) 2004-05-04
US20100317535A1 (en) 2010-12-16
KR20030092009A (ko) 2003-12-03
WO2002070736A3 (de) 2003-09-12
WO2002070736A8 (de) 2003-01-23
ATA3372001A (de) 2002-09-15
KR100892184B1 (ko) 2009-04-07
PL365022A1 (en) 2004-12-27

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Legal Events

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