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ES2164599B1 - Promotor y secuencias reguladoras de end1, un gen de guisante que se expresa especificamente en anteras - Google Patents

Promotor y secuencias reguladoras de end1, un gen de guisante que se expresa especificamente en anteras

Info

Publication number
ES2164599B1
ES2164599B1 ES200000814A ES200000814A ES2164599B1 ES 2164599 B1 ES2164599 B1 ES 2164599B1 ES 200000814 A ES200000814 A ES 200000814A ES 200000814 A ES200000814 A ES 200000814A ES 2164599 B1 ES2164599 B1 ES 2164599B1
Authority
ES
Spain
Prior art keywords
gene
end1
promoter
regulatory sequences
specifically expressed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200000814A
Other languages
English (en)
Other versions
ES2164599A1 (es
Inventor
Jimenez M Dolores Gomez
Clemente Luis Antonio Canas
Albi Francisco Madueno
Porter Jose Pio Beltran
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES200000814A priority Critical patent/ES2164599B1/es
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES01915442T priority patent/ES2372476T3/es
Priority to BRPI0107539-0A priority patent/BR0107539A/pt
Priority to US10/240,299 priority patent/US7078593B2/en
Priority to CNB018088678A priority patent/CN100422335C/zh
Priority to AU2001242538A priority patent/AU2001242538B2/en
Priority to EP01915442A priority patent/EP1291433B1/en
Priority to CNA2006101485221A priority patent/CN101089183A/zh
Priority to AT01915442T priority patent/ATE521709T1/de
Priority to PCT/ES2001/000127 priority patent/WO2001073088A1/es
Priority to MXPA02009612A priority patent/MXPA02009612A/es
Priority to AU4253801A priority patent/AU4253801A/xx
Publication of ES2164599A1 publication Critical patent/ES2164599A1/es
Application granted granted Critical
Publication of ES2164599B1 publication Critical patent/ES2164599B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pregnancy & Childbirth (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Promotor y secuencias reguladoras de END1, un gen de guisante que se expresa específicamente en anteras. Con la presente invención caracterizamos el promotor del gen END1 de guisante que es capaz de dirigir la expresión específica de antera en estadios tempranos de su desarrollo. Por otro lado, se indica su uso para producir plantas transgénicas que expresen un gen específico en antera, por ejemplo, un gen que provoque la ablación de la antera y por tanto diera lugar a una planta androestéril. Estas plantas androestériles son de gran utilidad en programas de mejora genética para la obtención de semillas híbridas.
ES200000814A 2000-03-31 2000-03-31 Promotor y secuencias reguladoras de end1, un gen de guisante que se expresa especificamente en anteras Expired - Fee Related ES2164599B1 (es)

Priority Applications (12)

Application Number Priority Date Filing Date Title
ES200000814A ES2164599B1 (es) 2000-03-31 2000-03-31 Promotor y secuencias reguladoras de end1, un gen de guisante que se expresa especificamente en anteras
AT01915442T ATE521709T1 (de) 2000-03-31 2001-03-30 Die antheren-spezifische expression eines gens regulierende sequenz und deren anwendung in der herstellung einer antheren-sterilen pflanze und hybriden samen
US10/240,299 US7078593B2 (en) 2000-03-31 2001-03-30 Sequence regulating the anther-specific expression of a gene and its use in the production of androsterile plants and hybrid seeds
CNB018088678A CN100422335C (zh) 2000-03-31 2001-03-30 调控基因的花药特异性表达的序列及其在生产雄性不育植物和杂种种子中的应用
AU2001242538A AU2001242538B2 (en) 2000-03-31 2001-03-30 Sequence regulating the anther-specific expression of a gene and its use in the production of androsterile plants and hybrid seeds
EP01915442A EP1291433B1 (en) 2000-03-31 2001-03-30 Sequence regulating the anther-specific expression of a gene and its use in the production of androsterile plants and hybrid seeds
ES01915442T ES2372476T3 (es) 2000-03-31 2001-03-30 Secuencia reguladora de la expresión específica en antera de un gen y su empleo en la producción de plantas androestériles y semillas híbridas.
BRPI0107539-0A BR0107539A (pt) 2000-03-31 2001-03-30 seqüência de nucleotìdeos que regula a expressão especìfica em anteras de um gene, constructo de dna, vetor recombinante, célula transformada, célula transgenética, planta transgenética, uso de uma planta transgenética, método para produção de plantas androestéreis e método para restaurar a fertilidade de uma planta androestéril
PCT/ES2001/000127 WO2001073088A1 (es) 2000-03-31 2001-03-30 Secuencia reguladora de la expresion especifica en antera de un gen y su empleo en la produccion de plantas androesteriles y semillas hibridas
MXPA02009612A MXPA02009612A (es) 2000-03-31 2001-03-30 Secuencia reguladora de la expresion especifica en antera de un gen y su empleo en la produccion de plantas androesteriles y semillas hibridas.
AU4253801A AU4253801A (en) 2000-03-31 2001-03-30 Sequence regulating the anther-specific expression of a gene and its use in the production of androsterile plants and hybrid seeds
CNA2006101485221A CN101089183A (zh) 2000-03-31 2001-03-30 调控基因的花药特异性表达的序列及其在生产雄性不育植物和杂种种子中的应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200000814A ES2164599B1 (es) 2000-03-31 2000-03-31 Promotor y secuencias reguladoras de end1, un gen de guisante que se expresa especificamente en anteras

Publications (2)

Publication Number Publication Date
ES2164599A1 ES2164599A1 (es) 2002-02-16
ES2164599B1 true ES2164599B1 (es) 2003-05-16

Family

ID=8492976

Family Applications (2)

Application Number Title Priority Date Filing Date
ES200000814A Expired - Fee Related ES2164599B1 (es) 2000-03-31 2000-03-31 Promotor y secuencias reguladoras de end1, un gen de guisante que se expresa especificamente en anteras
ES01915442T Expired - Lifetime ES2372476T3 (es) 2000-03-31 2001-03-30 Secuencia reguladora de la expresión específica en antera de un gen y su empleo en la producción de plantas androestériles y semillas híbridas.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES01915442T Expired - Lifetime ES2372476T3 (es) 2000-03-31 2001-03-30 Secuencia reguladora de la expresión específica en antera de un gen y su empleo en la producción de plantas androestériles y semillas híbridas.

Country Status (9)

Country Link
US (1) US7078593B2 (es)
EP (1) EP1291433B1 (es)
CN (2) CN101089183A (es)
AT (1) ATE521709T1 (es)
AU (2) AU2001242538B2 (es)
BR (1) BR0107539A (es)
ES (2) ES2164599B1 (es)
MX (1) MXPA02009612A (es)
WO (1) WO2001073088A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2265232B1 (es) * 2004-07-17 2008-06-16 Newbiotechnic, S.A. Tomates partenocarpicos y procedimiento para su produccion.
WO2006080598A1 (en) * 2005-01-31 2006-08-03 Dongbu Hitek Co., Ltd. Anther-specific genes derived from malus x domestica, promoters thereof and a method for preparing transformed plant using the same
CN100366744C (zh) * 2005-09-15 2008-02-06 复旦大学 蝴蝶兰pPI15编码序列及其应用
ES2319842B1 (es) * 2007-03-08 2010-02-16 Consejo Superior De Investigaciones Cientificas Procedimiento para modificar la arquitectura de la inflorescencia de las plantas.
CN101948861B (zh) * 2010-08-26 2012-07-04 北京大学 植物雄性不育系及其恢复系的培育方法
CN111154756B (zh) * 2020-01-07 2024-01-02 深圳市作物分子设计育种研究院 植物花药花粉发育后期特异性表达启动子及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5689041A (en) * 1989-08-10 1997-11-18 Plant Gentic Systems N.V. Plants modified with barstar for fertility restoration
ES2250961T3 (es) * 1992-06-12 2006-04-16 Bayer Bioscience N.V. Mantenimiento de plantas con esterilidad masculina.
EP0578611B1 (en) * 1992-07-02 2003-11-26 Syngenta Participations AG Anther-specific cDNA sequences, genomic DNA sequences and recombinant DNA sequences
AU715758B2 (en) * 1995-02-21 2000-02-10 Bayer Cropscience Nv Method to obtain male-sterile plants

Also Published As

Publication number Publication date
EP1291433A1 (en) 2003-03-12
CN1427893A (zh) 2003-07-02
WO2001073088A1 (es) 2001-10-04
US20040083504A1 (en) 2004-04-29
ES2372476T3 (es) 2012-01-20
AU2001242538B2 (en) 2007-02-08
MXPA02009612A (es) 2004-05-14
AU4253801A (en) 2001-10-08
CN101089183A (zh) 2007-12-19
US7078593B2 (en) 2006-07-18
ATE521709T1 (de) 2011-09-15
ES2164599A1 (es) 2002-02-16
BR0107539A (pt) 2006-08-29
CN100422335C (zh) 2008-10-01
EP1291433B1 (en) 2011-08-24

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