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ES2122292T3 - Diagnosis precoz de sepsis utilizando interacciones antigeno-anticuerpo amplificadas por quimioluminiscencia de sangre completa. - Google Patents

Diagnosis precoz de sepsis utilizando interacciones antigeno-anticuerpo amplificadas por quimioluminiscencia de sangre completa.

Info

Publication number
ES2122292T3
ES2122292T3 ES94918705T ES94918705T ES2122292T3 ES 2122292 T3 ES2122292 T3 ES 2122292T3 ES 94918705 T ES94918705 T ES 94918705T ES 94918705 T ES94918705 T ES 94918705T ES 2122292 T3 ES2122292 T3 ES 2122292T3
Authority
ES
Spain
Prior art keywords
antigen
test sample
infection
amount
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94918705T
Other languages
English (en)
Inventor
Alex D Romaschin
Paul M Walker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Critichem Medical Products Ltd
Original Assignee
Critichem Medical Products Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Critichem Medical Products Ltd filed Critical Critichem Medical Products Ltd
Application granted granted Critical
Publication of ES2122292T3 publication Critical patent/ES2122292T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN METODO PARA DETERMINAR EL GRADO DE INFECCION EN UN PACIENTE HUMANO O ANIMAL MEDIANTE LA DETECCION DE LA CANTIDAD DE UN INDICATIVO ANTIGENO DE TAL INFECCION; LA CANTIDAD DEL ANTIGENO SE DETECTA EN LA SANGRE DEL PACIENTE DERIVADA DE UNA MUESTRA QUE CONTIENE FRACCIONES DE CELULAS SANGUINEAS. EL METODO CONSTA DE: I) INCUBAR LA MUESTRA DE PRUEBA CON UNA CANTIDAD DE ANTICUERPOS DE PRUEBA ESPECIFICOS AL ANTIGENO INDICATIVO DE LA INFECCION PARA FORMAR COMPLEJOS DE ANTICUERPOS/ANTIGENOS QUE SON INSOLUBLES EN LA MUESTRA; II) PERMITIR QUE LOS COMPLEJOS DE ANTICUERPOS/ANTIGENOS INTERACTUEN CON LAS FRACCIONES DE GLOBULOS BLANCOS QUE RESULTAN EN LA PRODUCCION DE OXIDANTES; III) INTRODUCIR AL PASO I) O II) UN COMPUESTO QUIMILUMINESCENTE A LA MUESTRA DE PRUEBA; IV) LOS OXIDANTES QUE REACCIONAN CON LOS COMPUESTOS QUIMILUMINESCENTES PARA EMITIR UNA LUZ LUMINESCENTE DE LA MUESTRA DE PRUEBA; V) MEDIR LA CANTIDAD DE LUZ EMITIDA DURANTE UN PERIODO DE TIEMPO PREDETERMINADO; Y VI) CORRELACIONAR EL GRADO DE LA INFECCION MEDIANTE LA COMPARACION DE LA CANTIDAD MEDIDA DE LUZ EMITIDA DE LA MUESTRA DE PRUEBA CON UNA CANTIDAD MEDIDA DE LUZ EMITIDA POR UNA MUESTRA DE CONTROL QUE ES TRATADA DE LA MISMA FORMA QUE LA MUESTRA DE PRUEBA PARA LOS PASOS I) A V), EXCEPTO QUE EN EL PASO I) LOS ANTICUERPOS DE CONTROL QUE SE UTILIZAN SON DE LA MISMA CLASE QUE LOS ANTICUERPOS DE PRUEBA PERO NO SON ESPECIFICOS A LOS ANTIGENOS INDICATIVOS DE LA INFECCION.
ES94918705T 1993-06-08 1994-06-08 Diagnosis precoz de sepsis utilizando interacciones antigeno-anticuerpo amplificadas por quimioluminiscencia de sangre completa. Expired - Lifetime ES2122292T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002097952A CA2097952C (en) 1993-06-08 1993-06-08 Early diagnosis of sepsis utilizing antigen-antibody interactions amplified by whole blood chemiluminescence

Publications (1)

Publication Number Publication Date
ES2122292T3 true ES2122292T3 (es) 1998-12-16

Family

ID=4151762

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94918705T Expired - Lifetime ES2122292T3 (es) 1993-06-08 1994-06-08 Diagnosis precoz de sepsis utilizando interacciones antigeno-anticuerpo amplificadas por quimioluminiscencia de sangre completa.

Country Status (6)

Country Link
EP (1) EP0705434B1 (es)
AT (1) ATE172543T1 (es)
CA (1) CA2097952C (es)
DE (1) DE69414110T2 (es)
ES (1) ES2122292T3 (es)
WO (1) WO1994029728A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203997B1 (en) 1994-06-08 2001-03-20 Sepsis, Inc. Quantitation of analytes in whole blood
US6306614B1 (en) * 1994-06-08 2001-10-23 Sepsis, Inc. Measurement of analytes in whole blood
US5804370A (en) * 1994-06-08 1998-09-08 Critichem Medical Products Limited Early diagnosis of sepsis utilizing antigen-antibody interactions amplified by whole blood chemiluminescence
US6159683A (en) * 1997-12-16 2000-12-12 Spectral Diagnostics, Inc. Method of determining stage of sepsis
FI115165B (fi) * 2001-06-04 2005-03-15 Aboatech Ab Oy Menetelmä infektion laadun määrittämiseksi
DE102017003312A1 (de) 2017-04-05 2018-10-11 Rolf Günther Nachweisverfahren und Vorrichtung
JP2023554149A (ja) * 2020-12-22 2023-12-26 セロクソ リミテッド 敗血症を診断およびモニターする方法
CN114612799B (zh) * 2022-03-11 2022-09-16 应急管理部国家自然灾害防治研究院 基于滑坡/非滑坡面积比的空间自适应正负样本生成方法及系统

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1210328A (en) * 1982-12-14 1986-08-26 James J. Capone Method and composition for the evaluation of phagocytic response
JPS60200167A (ja) * 1984-03-24 1985-10-09 Toyobo Co Ltd 化学発光法による過酸化水素の定量法
US4959302A (en) * 1987-08-20 1990-09-25 Endogen, Inc. Method of evaluating immune system response to allografts, viral infection and immunosuppressive therapy
US5223395A (en) * 1988-12-01 1993-06-29 Centocor, Inc. Immunometric assays for tumor necrosis factor-alpha and methods for preventing the loss of biological activity of tumor necrosis factor-alpha in biological samples
US5294541A (en) * 1989-09-21 1994-03-15 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Real-time monitoring of oxidative products from in vitro cell-biomaterial interaction using chemiluminescence
US5108899A (en) * 1989-10-31 1992-04-28 Exoxemis, Inc. Chemiluminescence assay of in vivo inflammation
WO1992003734A1 (en) * 1990-08-20 1992-03-05 Alain De Weck A method for measuring t-lymphocyte responses by chemiluminescent assays
EP1681305A3 (en) * 1991-03-18 2008-02-27 New York University Monoclonal and chimeric antibodies specific for human tumor necrosis factor

Also Published As

Publication number Publication date
EP0705434B1 (en) 1998-10-21
DE69414110D1 (de) 1998-11-26
CA2097952A1 (en) 1994-12-09
EP0705434A1 (en) 1996-04-10
DE69414110T2 (de) 1999-06-10
AU684291B2 (en) 1997-12-11
ATE172543T1 (de) 1998-11-15
CA2097952C (en) 2006-03-14
WO1994029728A1 (en) 1994-12-22
AU6992394A (en) 1995-01-03

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