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ES2093294T3 - Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol. - Google Patents

Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol.

Info

Publication number
ES2093294T3
ES2093294T3 ES93103040T ES93103040T ES2093294T3 ES 2093294 T3 ES2093294 T3 ES 2093294T3 ES 93103040 T ES93103040 T ES 93103040T ES 93103040 T ES93103040 T ES 93103040T ES 2093294 T3 ES2093294 T3 ES 2093294T3
Authority
ES
Spain
Prior art keywords
promoter
glycerol
methanol
vector
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93103040T
Other languages
English (en)
Inventor
Yasuyoshi Sakai
Yoshiki Tani
Yuji Shibano
Hiroto Kondo
Haruyo Hatanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suntory Ltd
Original Assignee
Suntory Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Ltd filed Critical Suntory Ltd
Application granted granted Critical
Publication of ES2093294T3 publication Critical patent/ES2093294T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Adhesives Or Adhesive Processes (AREA)

Abstract

LA INVENCION SE REFIERE A UN VECTOR DE EXPRESION QUE SE INDUCE MEDIANTE METANOL Y/O GLICEROL UTILIZANDO UN GEL DE OXIDASA DE ALCOHOL DE UNA LEVADURA METILOTROFICA; A UNA LEVADURA METILOTROFICA RECOMBINANTE QUE CONTIENE DICHO VECTOR Y QUE ES CAPAZ DE ACUMULAR UNA CANTIDAD SIGNIFICATIVAMENTE ALTA DE UN PRODUCTO DE EXPRESION DE UN GEN HETEROLOGO; Y A UN METODO PARA PRODUCIR PRODUCTOS UTILES QUE UTILIZAN DICHO RECOMBINANTE. EL VECTOR DE EXPRESION SE CONSTRUYE UTILIZANDO EL PROMOTOR Y EL TERMINADOR DE UN GEN DE OXIDASA DE ALCOHOL DE LEVADURA METILOTROFICA. ADEMAS, SE PRODUCE UNA CANTIDAD SIGNIFICATIVAMENTE ALTA DE QUINASA DE ADENILATO, CITOCROMO C552 O PEROXIDASA USANDO DICHO VECTOR DE EXPRESION.
ES93103040T 1992-02-28 1993-02-26 Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol. Expired - Lifetime ES2093294T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4336192 1992-02-28

Publications (1)

Publication Number Publication Date
ES2093294T3 true ES2093294T3 (es) 1996-12-16

Family

ID=12661721

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93103040T Expired - Lifetime ES2093294T3 (es) 1992-02-28 1993-02-26 Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol.

Country Status (8)

Country Link
US (1) US5750372A (es)
EP (1) EP0558024B1 (es)
JP (2) JP3113435B2 (es)
AT (1) ATE141330T1 (es)
DE (1) DE69303943T2 (es)
DK (1) DK0558024T3 (es)
ES (1) ES2093294T3 (es)
GR (1) GR3020824T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3794748B2 (ja) 1996-03-04 2006-07-12 第一アスビオファーマ株式会社 メタノール代謝系を有する微生物の培養法
CA2198968C (en) * 1996-03-04 2010-02-09 Toyofumi Masuda Process for production of secretory kex2 derivatives
CN1192103C (zh) * 1998-01-27 2005-03-09 三井化学株式会社 生产植酸酶的方法
ATE338817T1 (de) 1998-02-19 2006-09-15 Kirin Brewery Promotor der dihydroxyacetonsynthase aus candida biodinii
AU1219000A (en) * 1998-10-22 2000-05-08 Regents Of The University Of California, The Functionally assembled antigen-specific intact recombinant antibody and a methodfor production thereof
KR100419530B1 (ko) * 2000-11-15 2004-02-19 주식회사 알엔에이 재조합 메탄올 자화 효모를 이용한 인간 페리틴의 대량생산 방법
US7892792B2 (en) * 2008-06-27 2011-02-22 Indian Institute Of Science Cells expressing Pichia cytochrome C

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2473927A1 (fr) * 1980-01-18 1981-07-24 Somalor Ferrari Somafer Ets Chalumeau decriqueur multiforme
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
DE69133066T2 (de) * 1990-01-16 2003-03-06 Centro De Ingenieria Genetica Y Biotecnologia, Cubanacan Playa Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen

Also Published As

Publication number Publication date
GR3020824T3 (en) 1996-11-30
JP3113435B2 (ja) 2000-11-27
DE69303943T2 (de) 1997-01-30
EP0558024B1 (en) 1996-08-14
JP2000279187A (ja) 2000-10-10
EP0558024A3 (en) 1993-12-29
JPH05344895A (ja) 1993-12-27
DE69303943D1 (de) 1996-09-19
DK0558024T3 (da) 1996-11-18
JP3137627B2 (ja) 2001-02-26
ATE141330T1 (de) 1996-08-15
EP0558024A2 (en) 1993-09-01
US5750372A (en) 1998-05-12

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