ES2009501A6 - Method for the purification of a biologically active agent - Google Patents
Method for the purification of a biologically active agentInfo
- Publication number
- ES2009501A6 ES2009501A6 ES8703511A ES8703511A ES2009501A6 ES 2009501 A6 ES2009501 A6 ES 2009501A6 ES 8703511 A ES8703511 A ES 8703511A ES 8703511 A ES8703511 A ES 8703511A ES 2009501 A6 ES2009501 A6 ES 2009501A6
- Authority
- ES
- Spain
- Prior art keywords
- freeze
- polar solvent
- factor
- dried product
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000013543 active substance Substances 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 239000002798 polar solvent Substances 0.000 abstract 4
- 239000000047 product Substances 0.000 abstract 3
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000012074 organic phase Substances 0.000 abstract 2
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 239000008346 aqueous phase Substances 0.000 abstract 1
- 210000000481 breast Anatomy 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
Landscapes
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Method for the purification of a biologically active agent. The method is characterized by comprising: shaking the cystic fluid of female breasts, type 1, with a first polar solvent, and discarding the precipitate mixing the dissolved factor with a second polar solvent, and recovering an organic phase which contains said factor said second polar solvent being less polar than the first solvent separating the second polar solvent from said organic phase freeze-drying the aqueous phase extracting the freeze-dried product with a methanol-water mixture extracting the freeze-dried product with a methanol-water mixture subjecting the extracted freeze- dried product to hydrophobic affinity chromatography and recovering the fractions which contain said factor.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US93955286A | 1986-12-09 | 1986-12-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2009501A6 true ES2009501A6 (en) | 1989-10-01 |
Family
ID=25473370
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES8901827A Expired - Lifetime ES2012007A6 (en) | 1986-12-09 | 1987-12-09 | Method for the purification of a biologically active agent |
| ES8703511A Expired ES2009501A6 (en) | 1986-12-09 | 1987-12-09 | Method for the purification of a biologically active agent |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES8901827A Expired - Lifetime ES2012007A6 (en) | 1986-12-09 | 1987-12-09 | Method for the purification of a biologically active agent |
Country Status (6)
| Country | Link |
|---|---|
| KR (1) | KR890700027A (en) |
| AR (1) | AR247098A1 (en) |
| ES (2) | ES2012007A6 (en) |
| HU (1) | HU201874B (en) |
| IL (1) | IL84745A (en) |
| ZA (1) | ZA879256B (en) |
-
1987
- 1987-12-07 IL IL84745A patent/IL84745A/en not_active IP Right Cessation
- 1987-12-07 AR AR87309522A patent/AR247098A1/en active
- 1987-12-07 HU HU88314A patent/HU201874B/en not_active IP Right Cessation
- 1987-12-07 KR KR1019880700950A patent/KR890700027A/en not_active Application Discontinuation
- 1987-12-09 ES ES8901827A patent/ES2012007A6/en not_active Expired - Lifetime
- 1987-12-09 ZA ZA879256A patent/ZA879256B/en unknown
- 1987-12-09 ES ES8703511A patent/ES2009501A6/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ZA879256B (en) | 1988-08-31 |
| HU201874B (en) | 1991-01-28 |
| IL84745A0 (en) | 1988-05-31 |
| AR247098A1 (en) | 1994-11-30 |
| KR890700027A (en) | 1989-03-02 |
| IL84745A (en) | 1992-12-01 |
| ES2012007A6 (en) | 1990-02-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| SA6 | Expiration date (snapshot 920101) |
Free format text: 2007-12-09 |