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EP2334802A4 - Methods of generating gene specific libraries - Google Patents

Methods of generating gene specific libraries

Info

Publication number
EP2334802A4
EP2334802A4 EP09813548A EP09813548A EP2334802A4 EP 2334802 A4 EP2334802 A4 EP 2334802A4 EP 09813548 A EP09813548 A EP 09813548A EP 09813548 A EP09813548 A EP 09813548A EP 2334802 A4 EP2334802 A4 EP 2334802A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene specific
specific libraries
generating gene
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09813548A
Other languages
German (de)
French (fr)
Other versions
EP2334802A1 (en
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP2334802A1 publication Critical patent/EP2334802A1/en
Publication of EP2334802A4 publication Critical patent/EP2334802A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09813548A 2008-09-09 2009-09-09 Methods of generating gene specific libraries Withdrawn EP2334802A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9557908P 2008-09-09 2008-09-09
PCT/US2009/056380 WO2010030683A1 (en) 2008-09-09 2009-09-09 Methods of generating gene specific libraries

Publications (2)

Publication Number Publication Date
EP2334802A1 EP2334802A1 (en) 2011-06-22
EP2334802A4 true EP2334802A4 (en) 2012-01-25

Family

ID=42005453

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09813548A Withdrawn EP2334802A4 (en) 2008-09-09 2009-09-09 Methods of generating gene specific libraries

Country Status (3)

Country Link
EP (1) EP2334802A4 (en)
CN (1) CN102203273A (en)
WO (1) WO2010030683A1 (en)

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WO2011156529A2 (en) * 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
CN102409408B (en) * 2010-09-21 2013-08-07 深圳华大基因科技服务有限公司 Method for accurate detection of whole genome methylation sites by utilizing trace genome DNA (deoxyribonucleic acid)
CN102409043B (en) * 2010-09-21 2013-12-04 深圳华大基因科技服务有限公司 Method for constructing high-flux and low-cost Fosmid library, label and label joint used in method
WO2012175013A1 (en) * 2011-06-24 2012-12-27 深圳华大基因科技有限公司 System and method for diagnosing human body with abnormal state
WO2013075313A1 (en) * 2011-11-24 2013-05-30 深圳华大基因科技有限公司 Probe for detecting method of integration of virus in test sample and preparation method and use thereof
BR112014001699A2 (en) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd method for sequencing a double stranded target polynucleotide, kit, methods for preparing a double stranded target polynucleotide for sequencing and sequencing a double stranded target polynucleotide, and apparatus
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
SG11201408478QA (en) 2012-06-18 2015-02-27 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
WO2014071361A1 (en) * 2012-11-05 2014-05-08 Rubicon Genomics Barcoding nucleic acids
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN110819621B (en) * 2014-02-11 2024-03-26 豪夫迈·罗氏有限公司 Targeted sequencing and UID filtration
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015200378A1 (en) * 2014-06-23 2015-12-30 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
WO2016022833A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
CN107075513B (en) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 Isolated oligonucleotides and their use in nucleic acid sequencing
EP3227476B1 (en) * 2014-12-02 2021-02-24 Tribiotica Llc Methods and kits for theranostic applications
CA2971589C (en) 2014-12-18 2021-09-28 Edico Genome Corporation Chemically-sensitive field effect transistor
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10640752B2 (en) * 2014-12-22 2020-05-05 Veterinærinstituttet Salmon gill poxvirus
CN104946639B (en) * 2015-07-01 2017-10-31 益善生物技术股份有限公司 Build the primer and method and kit of gene mutation sequencing library
CN108431233B (en) * 2015-11-11 2022-04-01 分析生物科学有限公司 Efficient construction of DNA libraries
US20170191127A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Droplet partitioned pcr-based library preparation
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109844137B (en) * 2016-10-31 2022-04-26 豪夫迈·罗氏有限公司 Barcoded circular library construction for identification of chimeric products
KR102569164B1 (en) * 2016-11-18 2023-08-21 트위스트 바이오사이언스 코포레이션 Polynucleotide libraries with controlled stoichiometry and their synthesis
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN109750092B (en) 2017-11-03 2022-12-06 北京贝瑞和康生物技术有限公司 Method and kit for targeted enrichment of target DNA with high GC content
CN108004301B (en) * 2017-12-15 2022-02-22 格诺思博生物科技南通有限公司 Gene target region enrichment method and library construction kit
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2612412B (en) * 2020-01-31 2025-10-10 Agilent Technologies Inc Systems and methods for targeted nucleic acid capture
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system

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EP1184466A2 (en) * 2000-08-26 2002-03-06 Affymetrix, Inc. Target nucleic acid enrichment and amplification for array analysis
WO2002083943A2 (en) * 2001-04-12 2002-10-24 Epigenomics Ag Microarray method for enriching dna fragments from complex mixtures
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

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WO2007018601A1 (en) * 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction

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EP1184466A2 (en) * 2000-08-26 2002-03-06 Affymetrix, Inc. Target nucleic acid enrichment and amplification for array analysis
WO2002083943A2 (en) * 2001-04-12 2002-10-24 Epigenomics Ag Microarray method for enriching dna fragments from complex mixtures
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

Non-Patent Citations (4)

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Title
JUDY ST. JOHN ET AL: "Rapid capture of DNA targets", BIOTECHNIQUES, vol. 44, no. 2, 1 February 2008 (2008-02-01), pages 259 - 264, XP055014746, ISSN: 0736-6205, DOI: 10.2144/000112633 *
OKOU DAVID T ET AL: "Microarray-based genomic selection for high-throughput resequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 4, no. 11, 1 October 2007 (2007-10-01), pages 907 - 909, XP002528498, ISSN: 1548-7091, DOI: 10.1038/NMETH1109 *
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Also Published As

Publication number Publication date
WO2010030683A1 (en) 2010-03-18
EP2334802A1 (en) 2011-06-22
CN102203273A (en) 2011-09-28

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