[go: up one dir, main page]

EP2334802A4 - Verfahren zur erstellung genspezifischer bibliotheken - Google Patents

Verfahren zur erstellung genspezifischer bibliotheken

Info

Publication number
EP2334802A4
EP2334802A4 EP09813548A EP09813548A EP2334802A4 EP 2334802 A4 EP2334802 A4 EP 2334802A4 EP 09813548 A EP09813548 A EP 09813548A EP 09813548 A EP09813548 A EP 09813548A EP 2334802 A4 EP2334802 A4 EP 2334802A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene specific
specific libraries
generating gene
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09813548A
Other languages
English (en)
French (fr)
Other versions
EP2334802A1 (de
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP2334802A1 publication Critical patent/EP2334802A1/de
Publication of EP2334802A4 publication Critical patent/EP2334802A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09813548A 2008-09-09 2009-09-09 Verfahren zur erstellung genspezifischer bibliotheken Withdrawn EP2334802A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9557908P 2008-09-09 2008-09-09
PCT/US2009/056380 WO2010030683A1 (en) 2008-09-09 2009-09-09 Methods of generating gene specific libraries

Publications (2)

Publication Number Publication Date
EP2334802A1 EP2334802A1 (de) 2011-06-22
EP2334802A4 true EP2334802A4 (de) 2012-01-25

Family

ID=42005453

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09813548A Withdrawn EP2334802A4 (de) 2008-09-09 2009-09-09 Verfahren zur erstellung genspezifischer bibliotheken

Country Status (3)

Country Link
EP (1) EP2334802A4 (de)
CN (1) CN102203273A (de)
WO (1) WO2010030683A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2391732B1 (de) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Verfahren die adaptoren zur nukleinsaure constructen für transmembrane sequenzierung benutzen
EP2580378A4 (de) * 2010-06-08 2014-01-01 Nugen Technologies Inc Verfahren und zusammensetzung für multiplex-sequenzierung
CN102409408B (zh) * 2010-09-21 2013-08-07 深圳华大基因科技服务有限公司 一种利用微量基因组dna进行全基因组甲基化位点精确检测的方法
CN102409043B (zh) * 2010-09-21 2013-12-04 深圳华大基因科技服务有限公司 高通量低成本Fosmid文库构建的方法及其所使用标签和标签接头
WO2013075313A1 (zh) * 2011-11-24 2013-05-30 深圳华大基因科技有限公司 一种检测病毒在待测样本中整合方式的探针及其制备方法和应用
WO2012175013A1 (zh) * 2011-06-24 2012-12-27 深圳华大基因科技有限公司 用于确定人体具有异常状态的系统和方法
KR20140050067A (ko) * 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP2861787B1 (de) 2012-06-18 2017-09-20 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur negativen auswahl von unerwünschten nukleinsäuresequenzen
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014071361A1 (en) * 2012-11-05 2014-05-08 Rubicon Genomics Barcoding nucleic acids
EP2964779B1 (de) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Verwendung von spacer-elementen in einer nukleinsäure zur kontrolle der bewegung einer helicase
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
EP3068883B1 (de) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung
CN106029962A (zh) * 2014-02-11 2016-10-12 豪夫迈·罗氏有限公司 靶向测序和uid过滤
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
AU2015280069B2 (en) * 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
SG11201700891SA (en) 2014-08-06 2017-03-30 Nugen Technologies Inc Digital measurements from targeted sequencing
JP6483249B2 (ja) * 2014-09-12 2019-03-13 エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． 単離されたオリゴヌクレオチドおよび核酸の配列決定におけるその使用
EP3227476B1 (de) * 2014-12-02 2021-02-24 Tribiotica Llc Verfahren und kits für theranostische anwendungen
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10429342B2 (en) 2014-12-18 2019-10-01 Edico Genome Corporation Chemically-sensitive field effect transistor
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2016102569A1 (en) * 2014-12-22 2016-06-30 Veterinærinstituttet Salmon gill poxvirus
CN104946639B (zh) * 2015-07-01 2017-10-31 益善生物技术股份有限公司 构建基因突变测序文库的引物和方法以及试剂盒
WO2017117440A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Droplet partitioned pcr-based library preparation
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109844137B (zh) * 2016-10-31 2022-04-26 豪夫迈·罗氏有限公司 用于鉴定嵌合产物的条形码化环状文库构建
KR102569164B1 (ko) * 2016-11-18 2023-08-21 트위스트 바이오사이언스 코포레이션 조절된 화학량론을 갖는 폴리뉴클레오티드 라이브러리 및 이의 합성
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN109750092B (zh) 2017-11-03 2022-12-06 北京贝瑞和康生物技术有限公司 一种靶向富集高gc含量目标dna的方法和试剂盒
CN108004301B (zh) * 2017-12-15 2022-02-22 格诺思博生物科技南通有限公司 基因目标区域富集方法及建库试剂盒
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2612412A (en) 2020-01-31 2023-05-03 Avida Biomed Inc Systems and methods for targeted nucleic acid capture
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1184466A2 (de) * 2000-08-26 2002-03-06 Affymetrix, Inc. Anreicherung und Amplifikation von Ziel-Nukleinsäuren für Array-Analyse
WO2002083943A2 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681726A (en) * 1988-09-19 1997-10-28 Stratagene Method of double stranded DNA synthesis
WO2002034949A2 (en) * 2000-10-27 2002-05-02 Molecular Staging Inc. Methods for identifying genes associated with diseases or specific phenotypes
GB0114853D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happier Mapping
EP1554386A2 (de) * 2002-07-24 2005-07-20 Immusol Incorporated Ein-promotor-system zur herstellung von sirna-expressionskassetten und -expressionsbibliotheken unter verwendung eines polymerase-primer-haarnadellinkers
WO2006007569A2 (en) * 2004-07-01 2006-01-19 Somagenics, Inc. Methods of preparation of gene-specific oligonucleotide libraries and uses thereof
EP1924704B1 (de) * 2005-08-02 2011-05-25 Rubicon Genomics, Inc. Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1184466A2 (de) * 2000-08-26 2002-03-06 Affymetrix, Inc. Anreicherung und Amplifikation von Ziel-Nukleinsäuren für Array-Analyse
WO2002083943A2 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
WO2007057652A1 (en) * 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JUDY ST. JOHN ET AL: "Rapid capture of DNA targets", BIOTECHNIQUES, vol. 44, no. 2, 1 February 2008 (2008-02-01), pages 259 - 264, XP055014746, ISSN: 0736-6205, DOI: 10.2144/000112633 *
OKOU DAVID T ET AL: "Microarray-based genomic selection for high-throughput resequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 4, no. 11, 1 October 2007 (2007-10-01), pages 907 - 909, XP002528498, ISSN: 1548-7091, DOI: 10.1038/NMETH1109 *
See also references of WO2010030683A1 *
UNN HILDE REFSETH ET AL: "HYBRIDIZATION CAPTURE OF MICROSATELLITES DIRECTLY FROM GENOMIC DNA", ELECTROPHORESIS, WILEY INTERSCIENCE, DE, vol. 18, no. 9, 1 January 1997 (1997-01-01), pages 1519 - 1523, XP002952426, ISSN: 0173-0835, DOI: 10.1002/ELPS.1150180905 *

Also Published As

Publication number Publication date
WO2010030683A1 (en) 2010-03-18
CN102203273A (zh) 2011-09-28
EP2334802A1 (de) 2011-06-22

Similar Documents

Publication Publication Date Title
EP2334802A4 (de) Verfahren zur erstellung genspezifischer bibliotheken
EP2126087A4 (de) Verfahren zur erstellung von bibliotheken und ihre verwendung
HK1223272A1 (zh) 程序性壞死的小分子抑制劑
EG25742A (en) Photobioreactor
HK1148220A1 (en) Methods of reducing eosinophil levels
HK1159250A1 (en) Improved method of rna display rna
HK1200487A1 (en) Silencing of csn5 gene expression using interfering rna rna csn5
EP2271952A4 (de) Visualisierung von geologischen merkmalen unter verwendung von datendarstellungen dieser
IL211758A0 (en) Authorization of server operations
IL212570A0 (en) Composiitons and methods for inhibiting expression of factor vii genes
ZA201102604B (en) Alkylcyclohexylethers of dihydrotetraazabenzoazulenes
ZA201006797B (en) Expression-linked gene discovery
ZA201305274B (en) Data analysis of dna sequences
PL2133419T3 (pl) Zastosowania beta-dipeptydów cyjanoficyny
GB0811500D0 (en) Method of determining DNA copy number
GB0807321D0 (en) Analysis of nucleic acids
GB0718542D0 (en) Targeted modulation of gene expression
EP2371126A4 (de) Zusammenführung mehrerer datensätze
GB0817384D0 (en) Method of providing location data
GB0701722D0 (en) Inhibition of gene expression
SI3309253T1 (sl) Geni za biosintezo tetraciklinskih spojin in uporabe le-teh
GB2487499B (en) Method of comparing audio data
EP2252701A4 (de) Verfahren zur identifikation eines organismus
GB0813688D0 (en) Method of determination
ZA201101308B (en) Improved synthesis of hexahydrodibenzopyranones

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20110408

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

AX Request for extension of the european patent

Extension state: AL BA RS

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20111223

RIC1 Information provided on ipc code assigned before grant

Ipc: C40B 50/06 20060101ALI20111219BHEP

Ipc: C40B 40/08 20060101ALI20111219BHEP

Ipc: C40B 40/06 20060101ALI20111219BHEP

Ipc: C12N 15/10 20060101ALI20111219BHEP

Ipc: C12N 15/66 20060101ALI20111219BHEP

Ipc: C12Q 1/68 20060101AFI20111219BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20120703