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EP2295581A3 - Mutants morphologiques de champignons filamenteux - Google Patents

Mutants morphologiques de champignons filamenteux Download PDF

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Publication number
EP2295581A3
EP2295581A3 EP10180181A EP10180181A EP2295581A3 EP 2295581 A3 EP2295581 A3 EP 2295581A3 EP 10180181 A EP10180181 A EP 10180181A EP 10180181 A EP10180181 A EP 10180181A EP 2295581 A3 EP2295581 A3 EP 2295581A3
Authority
EP
European Patent Office
Prior art keywords
cell
mutant
parent
filamentous fungi
identifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10180181A
Other languages
German (de)
English (en)
Other versions
EP2295581A2 (fr
Inventor
Jeffrey R. Shuster
John C. Royer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biotech Inc filed Critical Novozymes Biotech Inc
Publication of EP2295581A2 publication Critical patent/EP2295581A2/fr
Publication of EP2295581A3 publication Critical patent/EP2295581A3/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/77—Fusarium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP10180181A 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux Withdrawn EP2295581A3 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US1023896P 1996-01-19 1996-01-19
US72611496A 1996-10-04 1996-10-04
EP97904806A EP0877801B1 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux
EP05009106A EP1605050A3 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP97904806.3 Division 1997-07-24
EP05009106.5 Division 2005-04-26

Publications (2)

Publication Number Publication Date
EP2295581A2 EP2295581A2 (fr) 2011-03-16
EP2295581A3 true EP2295581A3 (fr) 2011-05-04

Family

ID=26680948

Family Applications (3)

Application Number Title Priority Date Filing Date
EP10180181A Withdrawn EP2295581A3 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux
EP05009106A Ceased EP1605050A3 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux
EP97904806A Expired - Lifetime EP0877801B1 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP05009106A Ceased EP1605050A3 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux
EP97904806A Expired - Lifetime EP0877801B1 (fr) 1996-01-19 1997-01-17 Mutants morphologiques de champignons filamenteux

Country Status (9)

Country Link
US (5) US6066493A (fr)
EP (3) EP2295581A3 (fr)
JP (1) JP3792725B2 (fr)
CN (1) CN1231588C (fr)
AT (1) ATE294233T1 (fr)
AU (1) AU1750697A (fr)
DE (1) DE69733121T2 (fr)
DK (1) DK0877801T3 (fr)
WO (1) WO1997026330A2 (fr)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3792725B2 (ja) * 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
DK1525798T3 (da) 1997-12-22 2012-03-05 Novozymes As Carbohydrat-oxidase og anvendelse deraf i bagning
EA005682B1 (ru) * 1998-10-06 2005-04-28 Марк Аарон Эмалфарб Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов
US6361973B1 (en) 1999-03-22 2002-03-26 Novozymes Biotech, Inc. Promoters for expressing genes in a fungal cell
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
US6995239B1 (en) * 1999-03-24 2006-02-07 Genencor International, Inc. Hyphal growth in fungi
US7189538B2 (en) * 1999-03-24 2007-03-13 Genencor International, Inc. Hyphal growth in fungi
ES2328011T3 (es) * 2000-04-13 2009-11-06 Dyadic International (Usa), Inc. Selecion de alto rendimiento de bibliotecas de adn expresadas en hongos filamentosos.
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
WO2002000852A2 (fr) 2000-06-26 2002-01-03 Novozymes A/S Lipase
US6558715B1 (en) * 2000-10-31 2003-05-06 Novozymes Biotech, Inc. Methods for using lipases in baking
US6824798B2 (en) * 2001-09-27 2004-11-30 J. Gregory Koenig Method of preventing veisalgia
WO2003089614A2 (fr) * 2002-04-18 2003-10-30 Genencor International, Inc. Production d'anticorps fonctionnels dans des champignons filamenteux
US8148155B2 (en) 2002-04-22 2012-04-03 Novozymes, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
DK1499733T3 (da) 2002-04-22 2010-07-12 Novozymes Inc Fremgangsmåder til fremstilling af varianter af en DNA-sekvens i trådformige svampe
ATE439432T1 (de) 2002-08-30 2009-08-15 Novozymes Inc Verfahren zur herstellung von säugetier-trypsinen
CN100480391C (zh) 2002-11-18 2009-04-22 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
DE60331477D1 (de) * 2002-12-24 2010-04-08 Ikeda Food Res Co Ltd Coenzymbindende glukosedehydrogenase
ES2347658T3 (es) 2003-05-02 2010-11-03 Novozymes Inc. Variantes de beta-glucosidasas.
CN101410514B (zh) 2003-08-25 2013-02-13 诺维信股份有限公司 糖苷水解酶的变体
JP4809347B2 (ja) 2004-06-14 2011-11-09 ノボザイムス アクティーゼルスカブ ポリペプチド製造用シグナルペプチド
US20060080393A1 (en) * 2004-10-12 2006-04-13 Cardone Richard J Method for using e-mail documents to create and update address lists
US9410175B2 (en) * 2005-03-24 2016-08-09 Dsm Ip Assets B.V. Process for microbial production of a valuable compound
WO2006101239A1 (fr) 2005-03-25 2006-09-28 Ikeda Food Research Co., Ltd. Glucose deshydrogenase liee a une co-enzyme et polynucleotide codant pour celle-ci
EP2505651A3 (fr) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolat de champignon avec activité protéase réduite
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
WO2008079228A1 (fr) * 2006-12-20 2008-07-03 Danisco Us, Inc., Genencor Division Analyes pour des souches fongiques améliorées
CN101679993A (zh) 2007-05-09 2010-03-24 诺维信公司 适于选择产生目标多肽的文库克隆的表达克隆方法
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
US8986974B2 (en) 2009-12-18 2015-03-24 Novozymes, Inc. Methods for producing polypeptidies in protease-deficient mutants of trichoderma
HUE035540T2 (en) * 2010-06-22 2018-05-02 Dsm Sinochem Pharm Nl Bv Air bubble fermentation process
BR112014004193A2 (pt) 2011-08-24 2017-03-28 Novozymes Inc célula hospedeira recombinante de trichoderma, métodos para produzir uma composição enzimática, composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação e para fermentar um material celulósico
WO2013028912A2 (fr) 2011-08-24 2013-02-28 Novozymes, Inc. Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse
BR112014004188A2 (pt) 2011-08-24 2017-03-21 Novozymes Inc método para obter transformantes positivos de uma célula hospedeira fúngica filamentosa, célula hospedeira fúngica filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
CA2846398A1 (fr) 2011-08-24 2013-02-28 Novozymes, Inc. Compositions d'enzymes cellulolytiques et leurs utilisations
EP3358000A3 (fr) 2012-01-05 2018-12-19 Glykos Finland Oy Cellules fongiques filamenteuses déficientes en protéases et leurs procédés d'utilisation
WO2013174927A1 (fr) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production de glycoprotéines fucosylées
CN104603269A (zh) 2012-08-17 2015-05-06 诺维信公司 用于在丝状真菌菌株中共沉默基因表达的方法及其应用
WO2014100612A1 (fr) 2012-12-21 2014-06-26 Novozymes, Inc. Procédés de production de polypeptides hétérologues dans des mutants de trichoderma
RU2016104064A (ru) 2013-07-10 2017-08-15 Новартис Аг Клетки мицелиальных грибов с множественной недостаточностью протеаз и способы их использования
EP3074513A1 (fr) 2013-11-26 2016-10-05 Novozymes A/S Compositions enzymatiques et leurs utilisations
AU2015269381B2 (en) 2014-06-06 2019-04-18 Novozymes A/S Enzyme compositions and uses thereof
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
WO2020123845A1 (fr) 2018-12-12 2020-06-18 Novozymes A/S Procédés pour augmenter la productivité d'une cellule fongique filamenteuse dans la production d'un polypeptide
BR112022014573A2 (pt) 2020-01-24 2022-10-04 Novozymes As Mutantes de uma célula fúngica filamentosa com produtividade aumentada na produção de um polipeptídeo

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0305216A1 (fr) * 1987-08-28 1989-03-01 Novo Nordisk A/S Lipase recombinante de humicola et procédé de production de lipases recombinantes de humicola
WO1995021910A1 (fr) * 1994-02-10 1995-08-17 Zeneca Limited Modification de micro-organismes filamenteux

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53118588A (en) * 1977-03-28 1978-10-17 Ajinomoto Co Inc Preparation of peptidase
US4288627A (en) 1980-02-12 1981-09-08 Phillips Petroleum Company Oxidation of thiols employing cobalt molybdate/triethylamine catalyst
US4816405A (en) * 1984-10-24 1989-03-28 The Regents Of The University Of California Vectors for transformation by ascomycetes
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
KR100237148B1 (ko) 1990-05-09 2000-01-15 한센 핀 베네드 엔도글루칸아제 효소를 함유하는 셀룰라제 제조물
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
PT765394E (pt) 1994-06-03 2002-03-28 Novozymes Biotech Inc Lacases myceliophthora purificadas e acidos nucleicos que as codificam
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
JP3792725B2 (ja) * 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
EP0966521A1 (fr) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cellules presentant des mutations d'insertion d'adn produisant des quantites modifiees d'un polypeptide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0305216A1 (fr) * 1987-08-28 1989-03-01 Novo Nordisk A/S Lipase recombinante de humicola et procédé de production de lipases recombinantes de humicola
WO1995021910A1 (fr) * 1994-02-10 1995-08-17 Zeneca Limited Modification de micro-organismes filamenteux

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
TRINCI, A.P.J.: "MYCO-PROTEIN: A TWENTY-YEAR OVERNIGHT SUCCESS STORY", MYCOL. RES., vol. 96, no. 1, 1992, pages 1 - 13, XP002036322 *
VAN DEN HONDEL, C.A.M.J.J., ET AL .: "PRODUCTION OF EXTRACELLULAR PROTEINS BY THE FILAMENTOUS FUNGUS ASPERGILLUS", ANTONIE VAN LEEUWENHOEK, vol. 61, 1992, pages 153 - 160, XP002036324 *
WIEBE, M.G., ET AL .: "CHARACTERIZATION OF MORPHOLOGICAL MUTANTS GENERATED SPONTANEOUSLY IN GLUCOSE-LIMITED, CONTINOUS FLOW CULTURES OF FUSARIUM GRAMINEARUM A3/5", MYCOL. RES., vol. 96, no. 7, 1992, pages 555 - 562, XP002036323 *
WITHERS, J.M., ET AL .: "DEVELOPMENT OF MORPHOLOGICAL HETEROGENEITY IN GLUCOSE-LIMITED CHEMOSTAT CULTURES OF ASPERGILLUS ORYZAE", MYCOL. RES., vol. 98, no. 1, 1994, pages 95 - 100, XP002036321 *

Also Published As

Publication number Publication date
US20040009579A1 (en) 2004-01-15
AU1750697A (en) 1997-08-11
EP1605050A2 (fr) 2005-12-14
US7198938B2 (en) 2007-04-03
EP2295581A2 (fr) 2011-03-16
DK0877801T3 (da) 2005-08-29
WO1997026330A2 (fr) 1997-07-24
CN1231588C (zh) 2005-12-14
EP0877801B1 (fr) 2005-04-27
DE69733121D1 (de) 2005-06-02
US6066493A (en) 2000-05-23
EP0877801A2 (fr) 1998-11-18
JP3792725B2 (ja) 2006-07-05
ATE294233T1 (de) 2005-05-15
WO1997026330A3 (fr) 1997-10-09
US6544774B1 (en) 2003-04-08
DE69733121T2 (de) 2006-03-02
US6184026B1 (en) 2001-02-06
EP1605050A3 (fr) 2006-03-22
CN1219971A (zh) 1999-06-16
US6794180B2 (en) 2004-09-21
US20040266009A1 (en) 2004-12-30
JP2000503537A (ja) 2000-03-28

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