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EP1256328B1 - Straw for the preservation of small amounts of substances, in particular of biological liquids - Google Patents

Straw for the preservation of small amounts of substances, in particular of biological liquids Download PDF

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Publication number
EP1256328B1
EP1256328B1 EP02291040.0A EP02291040A EP1256328B1 EP 1256328 B1 EP1256328 B1 EP 1256328B1 EP 02291040 A EP02291040 A EP 02291040A EP 1256328 B1 EP1256328 B1 EP 1256328B1
Authority
EP
European Patent Office
Prior art keywords
straw
stopper
gel powder
dispersed
straw according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP02291040.0A
Other languages
German (de)
French (fr)
Other versions
EP1256328B8 (en
EP1256328A1 (en
Inventor
Jean-Gérard Saint-Ramon
Francis Lesieur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IMV Technologies SA
Original Assignee
IMV Technologies SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IMV Technologies SA filed Critical IMV Technologies SA
Publication of EP1256328A1 publication Critical patent/EP1256328A1/en
Application granted granted Critical
Publication of EP1256328B1 publication Critical patent/EP1256328B1/en
Publication of EP1256328B8 publication Critical patent/EP1256328B8/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • A61D19/024—Tube-like containers, e.g. straws
    • A—HUMAN NECESSITIES
    • A46—BRUSHWARE
    • A46B—BRUSHES
    • A46B11/00—Brushes with reservoir or other means for applying substances, e.g. paints, pastes, water
    • A46B11/001—Brushes with reservoir or other means for applying substances, e.g. paints, pastes, water with integral reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Definitions

  • the present invention relates to a straw for the preservation of small amounts of substances, especially biological substances, in particular biological liquids.
  • the flakes manufactured according to this patent and used heretofore comprise a tripartite stopper constituted by two pads of a fibrous substance enclosing a powder capable of being transformed on contact with a liquid into an impermeable paste or gel adhering to the wall of the tube. by creating a waterproof cap.
  • the patent FR-A-2,753,367 proposes a straw comprising a tripartite plug comprising a hydrogel powder between two buffers, an external buffer and an internal buffer, said straw being characterized in that the length of the outer buffer is at least twice the length of the internal buffer. According to this patent the absorption of the liquid contained in the straw is only very slightly reduced compared to that observed with a straw provided with a conventional tripartite stopper.
  • the patent GB1,299,934 discloses a straw for the preservation of small amounts of substance consisting of a tube and a stopper comprising gelling powder surrounded by a cylindrical braid.
  • the patent GB1,192,946 discloses a straw provided with a cap and teaches various possibilities for elements replacing the braided textile material.
  • It may be a mixture of polyvinyl alcohol granules and cellulose ether granules, the latter granules having been subjected during their production to a controlled heat treatment so that they become less sensitive to water; it may also be a mixture of polyvinyl alcohol granules and polyvinyl chloride beads; and again the elements replacing the braided textile material may consist solely of polyvinyl chloride beads.
  • An object of the present invention is to provide a straw provided with a plug which while being liquid-tight, absorbs only a small amount of a liquid contained in the straw. More specifically, an object of the invention is to provide a straw provided with a sealing cap which absorbs only a quantity of a liquid contained therein at most equal to about 4%.
  • the present invention meets the above object and provides a straw for the preservation of small amounts of substances, especially biological substances, consisting of a tube segment provided inside one of its ends.
  • a plug comprising gelling powder and a braid or sheath, characterized in that a part of the gelling powder is replaced by non-absorbent material in dispersed form, said plug thus comprising a mixture of gelling powder and of non-absorbent material in dispersed form.
  • the gelling powder is a material capable of being transformed in contact with a liquid into an impermeable paste or gel adhering to the wall of the tube by creating a tight stopper.
  • the gelling powder may for example be an alginate or polyvinyl alcohol.
  • a part of the gelling powder is replaced by non-absorbent material in dispersed form.
  • the non-absorbent material may be, for example, glass, PVC, diatoms, perlites, etc.
  • the non-absorbent material must be in dispersed form.
  • the particle size of the non-absorbent material should be close to that of the gelling powder.
  • the particle size of the gelling powder to be used is easily determined by those skilled in the art according to the nature of said gelling powder.
  • the non-absorbent material is for example in the form of beads.
  • the beads have an average diameter of, for example, between about 50 and about 300 ⁇ m.
  • the non-absorbent material may also be in the form of small lamellae or fragments.
  • a proportion of gelling powder of between about 10% and about 50% is replaced by non-absorbent material in dispersed form.
  • the straw may be provided with a tripartite or monoblock plug.
  • the tripartite plug is constituted by an external part, which is not in direct contact with the liquid contained in the straw and an internal part, in contact with the liquid contained in the straw, enclosing an intermediate portion containing powder gelling.
  • These three parts may be substantially the same length or have different lengths.
  • the straw is provided with a conventional tripartite stopper.
  • This plug consists of two buffers of a fibrous substance enclosing a gelling powder. According to the invention, a portion of the gelling powder of the plug is replaced by non-absorbent material in dispersed form.
  • the outer portion is substantially fibrous standard and the inner portion is substantially fibrous standard.
  • the outer and inner parts may be in the form of a braid.
  • the intermediate part consists of a mixture of gelling powder and non-absorbent material in dispersed form.
  • the straw for the preservation of small quantities of substances, in particular biological substances consists of a tube segment provided inside one of its ends with a plug consisting of an outer portion and an inner portion enclosing an intermediate portion comprising the gelling powder. It is characterized in that the intermediate portion is composed of a mixture of a gelling powder and non-absorbent material in dispersed form.
  • the cap of the straw according to the invention is tripartite and comprises a core of thermoplastic material sheathed by son constituting a sheath.
  • the tripartite stopper consists of an outer part composed of a fibrous material braid, an inner part composed of a core of thermoplastic material sheathed by wires constituting a sheath and an intermediate portion composed of gelling powder in which, according to the invention, has been substituted part by non-absorbent material in dispersed form.
  • the intermediate portion consists of a mixture of gelling powder and non-absorbent material in dispersed form.
  • the tripartite stopper consists of an outer part composed of a fibrous material braid, an inner part composed of a core of thermoplastic material sheathed by son constituting a sheath. said core having a bore and an intermediate portion composed of gelling powder in which, according to the invention, a part has been substituted with non-absorbent material in dispersed form.
  • the intermediate portion consists of a mixture of gelling powder and non-absorbent material in dispersed form.
  • the tripartite stopper consists of an outer part composed of a fibrous substance braid, an inner part composed of a core of thermoplastic material sheathed by wires constituting a sheath, said core having a central braid, and an intermediate portion composed of gelling powder in which, according to the invention, has been substituted part by non-absorbent material in dispersed form.
  • the intermediate portion therefore consists of a mixture of gelling powder and non-absorbent material in dispersed form.
  • the cap is in one piece.
  • the one-piece plug consists of a core of thermoplastic material sheathed by son forming a sheath impregnated with a gelling powder in which, according to the invention, a part has been substituted with the non-absorbent material in dispersed form.
  • the one-piece plug consists of a core of thermoplastic material sheathed by son forming a sheath and comprising a central braid impregnated with gelling powder in which, according to the invention, has been substituted a partly by non-absorbent material in dispersed form.
  • the total length of the plug is between about 7 mm and about 14 mm.
  • the outer portion has a length of between about 3 mm and about 14 mm.
  • the inner portion has a length of between about 2 mm and about 5 mm.
  • the intermediate portion has a length of between about 1.5 mm and about 5 mm.
  • the amount of gelling powder that must be incorporated into the straw stopper before the implementation of the invention depends on the type of stopper and is known to those skilled in the art.
  • the proportion of gelling powder replaced by non-absorbent material in dispersed form between about 10% and about 50% and depends on the type of plug. Those skilled in the art will readily determine in each case the proportion of gelling powder to be replaced by non-absorbent material in dispersed form to obtain a straw with a sealed plug and reduced liquid absorption.
  • the straw of the invention has a good seal and a very low absorption of the liquid contained therein, of the order of 4% and even less.
  • the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.
  • the outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.
  • the inner portion 33 in contact with the liquid contained in the straw, is a braid of fibrous substance.
  • the intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.
  • the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.
  • the outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.
  • the inner part 33 in contact with the liquid contained in the straw, consists of a core 53 made of thermoplastic material sheathed by wires in a sheath 63.
  • the intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.
  • the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.
  • the outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.
  • the inner part 33 in contact with the liquid contained in the straw, consists of a core 53 of thermoplastic material sheathed by wires in a sheath 63.
  • the core 53 has a bore 73.
  • the intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.
  • the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.
  • the outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.
  • the inner part 33 in contact with the liquid contained in the straw, consists of a core 53 of thermoplastic material sheathed by wires in a sheath 63.
  • the core 53 comprises a central braid 83.
  • the intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.
  • the straw 1 consists of a tube segment 2 provided at one of its ends with a plug 3 monobloc.
  • the plug 3 consists of a core 50 of thermoplastic material sheathed by son forming a sheath 60.
  • the son of the sheath 60 are impregnated with a mixture 4 of gelling powder and non-absorbent material in dispersed form.
  • the straw 1 consists of a tube segment 2 provided at one of its ends with a plug 3 monobloc.
  • the plug 3 consists of a core 50 of thermoplastic material sheathed by son constituting a sheath 60 comprising a central braid 80 of synthetic son impregnated with a mixture 4 of gelling powder and non-absorbent material in dispersed form.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medical Preparation Storing Or Oral Administration Devices (AREA)
  • Insulated Conductors (AREA)
  • Absorbent Articles And Supports Therefor (AREA)
  • Fertilizing (AREA)
  • Media Introduction/Drainage Providing Device (AREA)
  • Packages (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
  • Closures For Containers (AREA)

Description

La présente invention concerne une paillette pour la conservation de petites quantités de substances, notamment des substances biologiques, en particulier des liquides biologiques.The present invention relates to a straw for the preservation of small amounts of substances, especially biological substances, in particular biological liquids.

Ce type de paillette, connue sous la dénomination de "paillette française", a été divulgué pour la première fois dans le Brevet Français N° 995 878 .This type of straw, known under the name of "French straw", was disclosed for the first time in the French Patent No. 995,878 .

Les paillettes fabriquées selon ce brevet et utilisées jusqu'à présent comprennent un bouchon tripartite constitué par deux tampons d'une substance fibreuse enserrant une poudre susceptible de se transformer au contact d'un liquide en une pâte ou gel imperméable adhérant à la paroi du tube en créant un bouchon étanche.The flakes manufactured according to this patent and used heretofore comprise a tripartite stopper constituted by two pads of a fibrous substance enclosing a powder capable of being transformed on contact with a liquid into an impermeable paste or gel adhering to the wall of the tube. by creating a waterproof cap.

Les paillettes à bouchon tripartite mises au point et commercialisées par la Demanderesse donnent toute satisfaction. Néanmoins, une certaine absorption de liquide contenu dans la paillette a pu être constatée, quoique très faible, de l'ordre de 7 %.The sequins tripartite plug developed and marketed by the Applicant are very satisfactory. Nevertheless, some absorption of liquid contained in the straw has been observed, albeit very low, of the order of 7%.

Une solution qui a été proposée pour pallier le problème ci-dessus a été de remplacer le bouchon tripartite par une bille en matière plastique rigide ou métallique. Cette solution ne s'est pas avérée satisfaisante car, si l'absorption de liquide contenu dans la paillette a été réduite à zéro, de nouvelles difficultés rédhibitoires sont apparues, le bouchon n'étant plus du tout étanche aux liquides.One solution that has been proposed to overcome the above problem has been to replace the tripartite plug with a rigid or metallic plastic ball. This solution was not satisfactory because, if the absorption of liquid contained in the straw has been reduced to zero, new unacceptable difficulties have appeared, the plug being no longer liquid-tight.

Le brevet FR-A-2 753 367 propose une paillette comportant un bouchon tripartite comprenant une poudre hydrogel entre deux tampons, tampon externe et un tampon interne, ladite paillette étant caractérisée en ce que la longueur du tampon externe est au moins deux fois supérieure à la longueur du tampon interne. Selon ce brevet l'absorption du liquide contenu dans la paillette n'est que très faiblement réduite par rapport à celle constatée avec une paillette munie d'un bouchon tripartite classique.The patent FR-A-2,753,367 proposes a straw comprising a tripartite plug comprising a hydrogel powder between two buffers, an external buffer and an internal buffer, said straw being characterized in that the length of the outer buffer is at least twice the length of the internal buffer. According to this patent the absorption of the liquid contained in the straw is only very slightly reduced compared to that observed with a straw provided with a conventional tripartite stopper.

Le brevet GB1,299,934 divulgue une paillette pour la conservation de petites quantités de substance se composant d'un tube et d'un bouchon comportant de la poudre gélifiante entourée par une tresse cylindrique.The patent GB1,299,934 discloses a straw for the preservation of small amounts of substance consisting of a tube and a stopper comprising gelling powder surrounded by a cylindrical braid.

Le brevet GB1,192,946 divulgue une paillette munie d'un bouchon et enseigne diverses possibilités pour les éléments venant en remplacement de la matière textile tressée.The patent GB1,192,946 discloses a straw provided with a cap and teaches various possibilities for elements replacing the braided textile material.

Il peut s'agir d'un mélange de granules d'alcool polyvinylique et de granules d'éther cellulose, ces derniers granules ayant été soumis pendant leur production à un traitement thermique contrôlé pour qu'ils deviennent moins sensibles à l'eau; il peut également s'agir d'un mélange de granules d'alcool polyvinylique et de perles de chlorure de polyvinyle; et encore les éléments venant en remplacement de la matière textile tressée peuvent être constitués uniquement de perles de chlorure de polyvinyle.It may be a mixture of polyvinyl alcohol granules and cellulose ether granules, the latter granules having been subjected during their production to a controlled heat treatment so that they become less sensitive to water; it may also be a mixture of polyvinyl alcohol granules and polyvinyl chloride beads; and again the elements replacing the braided textile material may consist solely of polyvinyl chloride beads.

Un objet de la présente invention est de disposer d'une paillette munie d'un bouchon qui tout en étant étanche aux liquides, n'absorbe qu'une faible quantité d'un liquide contenu dans la paillette. Plus précisément, un objet de l'invention est de fournir une paillette munie d'un bouchon étanche qui n'absorbe qu'une quantité d'un liquide qui y est contenu au plus égale à environ 4 %.An object of the present invention is to provide a straw provided with a plug which while being liquid-tight, absorbs only a small amount of a liquid contained in the straw. More specifically, an object of the invention is to provide a straw provided with a sealing cap which absorbs only a quantity of a liquid contained therein at most equal to about 4%.

D'autres objets et avantages de l'invention apparaîtront à la lecture de la description ci-après.Other objects and advantages of the invention will appear on reading the description below.

La présente invention répond à l'objet ci-dessus et fournit une paillette pour la conservation de petites quantités de substances, notamment des substances biologiques, se composant d'un segment de tube muni à l'intérieur de l'une de ses extrémités d'un bouchon comportant de la poudre gélifiante ainsi qu'une tresse ou une gaine, caractérisée en ce qu'une partie de la poudre gélifiante est remplacée par de la matière non absorbante sous forme dispersée, ledit bouchon comprenant ainsi un mélange de poudre gélifiante et de matière non absorbante sous forme dispersée.The present invention meets the above object and provides a straw for the preservation of small amounts of substances, especially biological substances, consisting of a tube segment provided inside one of its ends. a plug comprising gelling powder and a braid or sheath, characterized in that a part of the gelling powder is replaced by non-absorbent material in dispersed form, said plug thus comprising a mixture of gelling powder and of non-absorbent material in dispersed form.

La poudre gélifiante est une matière susceptible de se transformer au contact d'un liquide en une pâte ou gel imperméable adhérant à la paroi du tube en créant un bouchon étanche.The gelling powder is a material capable of being transformed in contact with a liquid into an impermeable paste or gel adhering to the wall of the tube by creating a tight stopper.

La poudre gélifiante peut par exemple être un alginate ou bien de l'alcool polyvinylique.The gelling powder may for example be an alginate or polyvinyl alcohol.

Selon l'invention, une partie de la poudre gélifiante est remplacée par de la matière non absorbante sous forme dispersée.According to the invention, a part of the gelling powder is replaced by non-absorbent material in dispersed form.

La matière non absorbante peut être par exemple du verre, du PVC, des diatomées, des perlites, etc.The non-absorbent material may be, for example, glass, PVC, diatoms, perlites, etc.

La matière non absorbante doit se trouver sous forme dispersée.The non-absorbent material must be in dispersed form.

La granulométrie de la matière non absorbante doit être voisine de celle de la poudre gélifiante.The particle size of the non-absorbent material should be close to that of the gelling powder.

La granulométrie de la poudre gélifiante à utiliser est déterminée aisément par l'homme du métier selon la nature de ladite poudre gélifiante.The particle size of the gelling powder to be used is easily determined by those skilled in the art according to the nature of said gelling powder.

La matière non absorbante est par exemple sous forme de billes.The non-absorbent material is for example in the form of beads.

Les billes ont un diamètre moyen compris par exemple entre environ 50 et environ 300 µm.The beads have an average diameter of, for example, between about 50 and about 300 μm.

La matière non absorbante peut également être sous forme de petites lamelles ou de fragments.The non-absorbent material may also be in the form of small lamellae or fragments.

Selon l'invention, une proportion de poudre gélifiante comprise entre environ 10% et environ 50% est remplacée par de la matière non absorbante sous forme dispersée.According to the invention, a proportion of gelling powder of between about 10% and about 50% is replaced by non-absorbent material in dispersed form.

Selon l'invention la paillette peut être munie d'un bouchon tripartite ou monobloc.According to the invention the straw may be provided with a tripartite or monoblock plug.

Le bouchon tripartite est constitué par une partie externe, qui n'est pas en contact direct avec le liquide contenu dans la paillette et d'une partie interne, en contact avec le liquide contenu dans la paillette, enserrant une partie intermédiaire contenant de la poudre gélifiante.The tripartite plug is constituted by an external part, which is not in direct contact with the liquid contained in the straw and an internal part, in contact with the liquid contained in the straw, enclosing an intermediate portion containing powder gelling.

Ces trois parties peuvent être sensiblement de même longueur ou avoir des longueurs différentes.These three parts may be substantially the same length or have different lengths.

Dans un premier mode de réalisation, la paillette est munie d'un bouchon tripartite classique. Ce bouchon est constitué par deux tampons d'une substance fibreuse enserrant une poudre gélifiante. Selon l'invention, une partie de la poudre gélifiante du bouchon est remplacée par de la matière non absorbante sous forme dispersée.In a first embodiment, the straw is provided with a conventional tripartite stopper. This plug consists of two buffers of a fibrous substance enclosing a gelling powder. According to the invention, a portion of the gelling powder of the plug is replaced by non-absorbent material in dispersed form.

Dans ce premier mode de réalisation, la partie externe est en substance fibreuse standard et la partie interne est en substance fibreuse standard.In this first embodiment, the outer portion is substantially fibrous standard and the inner portion is substantially fibrous standard.

Les parties externe et interne peuvent être sous forme d'une tresse.The outer and inner parts may be in the form of a braid.

La partie intermédiaire est constituée d'un mélange de poudre gélifiante et de matière non absorbante sous forme dispersée.The intermediate part consists of a mixture of gelling powder and non-absorbent material in dispersed form.

Dans le premier mode de réalisation, la paillette pour la conservation de petites quantités de substances, notamment des substances biologiques, se compose d'un segment de tube muni à l'intérieur de l'une de ses extrémités d'un bouchon constitué d'une partie externe et d'une partie interne enserrant une partie intermédiaire comportant de la poudre gélifiante. Elle est caractérisée en ce que la partie intermédiaire est composée d'un mélange d'une poudre gélifiante et de matière non absorbante sous forme dispersée.In the first embodiment, the straw for the preservation of small quantities of substances, in particular biological substances, consists of a tube segment provided inside one of its ends with a plug consisting of an outer portion and an inner portion enclosing an intermediate portion comprising the gelling powder. It is characterized in that the intermediate portion is composed of a mixture of a gelling powder and non-absorbent material in dispersed form.

Dans un deuxième mode de réalisation de l'invention, le bouchon de la paillette selon l'invention est tripartite et il comprend un noyau en matière thermoplastique gainé par des fils constituant une gaine.In a second embodiment of the invention, the cap of the straw according to the invention is tripartite and comprises a core of thermoplastic material sheathed by son constituting a sheath.

Dans un premier cas de ce deuxième mode de réalisation, le bouchon tripartite est constitué d'une partie externe composée d'une tresse en substance fibreuse, d'une partie interne composée d'un noyau en matière thermoplastique gainé par des fils en constituant une gaine et d'une partie intermédiaire composée de poudre gélifiante dans laquelle, selon l'invention, on a substitué une partie par de la matière non absorbante sous forme dispersée.In a first case of this second embodiment, the tripartite stopper consists of an outer part composed of a fibrous material braid, an inner part composed of a core of thermoplastic material sheathed by wires constituting a sheath and an intermediate portion composed of gelling powder in which, according to the invention, has been substituted part by non-absorbent material in dispersed form.

En d'autres termes, dans ce premier cas du deuxième mode de réalisation, la partie intermédiaire est constituée d'un mélange de poudre gélifiante et de matière non absorbante sous forme dispersée.In other words, in this first case of the second embodiment, the intermediate portion consists of a mixture of gelling powder and non-absorbent material in dispersed form.

Dans un deuxième cas du deuxième mode de réalisation, le bouchon tripartite est constitué d'une partie externe composée d'une tresse en substance fibreuse, d'une partie interne composée d'un noyau en matière thermoplastique gainé par des fils en constituant une gaine, ledit noyau comportant un alésage et d'une partie intermédiaire composée de poudre gélifiante dans laquelle, selon l'invention, on a substitué une partie par de la matière non absorbante sous forme dispersée.In a second case of the second embodiment, the tripartite stopper consists of an outer part composed of a fibrous material braid, an inner part composed of a core of thermoplastic material sheathed by son constituting a sheath. said core having a bore and an intermediate portion composed of gelling powder in which, according to the invention, a part has been substituted with non-absorbent material in dispersed form.

Ici encore, la partie intermédiaire est constituée d'un mélange de poudre gélifiante et de matière non absorbante sous forme dispersée.Here again, the intermediate portion consists of a mixture of gelling powder and non-absorbent material in dispersed form.

Dans un troisième cas de ce deuxième mode de réalisation, le bouchon tripartite est constitué d'une partie externe composée d'une tresse en substance fibreuse, d'une partie interne composée d'un noyau en matière thermoplastique gainé par des fils en constituant une gaine, ledit noyau comportant une tresse centrale, et d'une partie intermédiaire composée de poudre gélifiante dans laquelle, selon l'invention, on a substitué une partie par de la matière non absorbante sous forme dispersée.In a third case of this second embodiment, the tripartite stopper consists of an outer part composed of a fibrous substance braid, an inner part composed of a core of thermoplastic material sheathed by wires constituting a sheath, said core having a central braid, and an intermediate portion composed of gelling powder in which, according to the invention, has been substituted part by non-absorbent material in dispersed form.

La partie intermédiaire est donc constituée d'un mélange de poudre gélifiante et de matière non absorbante sous forme dispersée.The intermediate portion therefore consists of a mixture of gelling powder and non-absorbent material in dispersed form.

Dans un troisième mode de réalisation de l'invention, le bouchon est monobloc.In a third embodiment of the invention, the cap is in one piece.

Dans un premier cas du troisième mode de réalisation, le bouchon monobloc est constitué d'un noyau en matière thermoplastique gainé par des fils constituant une gaine imprégnés d'une poudre gélifiante dans laquelle, selon l'invention, on a substitué une partie par de la matière non absorbante sous forme dispersée.In a first case of the third embodiment, the one-piece plug consists of a core of thermoplastic material sheathed by son forming a sheath impregnated with a gelling powder in which, according to the invention, a part has been substituted with the non-absorbent material in dispersed form.

Dans un deuxième cas du troisième mode de réalisation, le bouchon monobloc est constitué d'un noyau en matière thermoplastique gainé par des fils constituant une gaine et comprenant une tresse centrale imprégnée de poudre gélifiante dans laquelle, selon l'invention, on a substitué une partie par de la matière non absorbante sous forme dispersée.In a second case of the third embodiment, the one-piece plug consists of a core of thermoplastic material sheathed by son forming a sheath and comprising a central braid impregnated with gelling powder in which, according to the invention, has been substituted a partly by non-absorbent material in dispersed form.

Dans le cas d'un bouchon tripartite, la longueur totale du bouchon est comprise entre environ 7 mm et environ 14 mm.In the case of a tripartite plug, the total length of the plug is between about 7 mm and about 14 mm.

La partie externe a une longueur comprise entre environ 3 mm et environ 14 mm.The outer portion has a length of between about 3 mm and about 14 mm.

La partie interne a une longueur comprise entre environ 2 mm et environ 5 mm.The inner portion has a length of between about 2 mm and about 5 mm.

La partie intermédiaire a une longueur comprise entre environ 1,5 mm et environ 5 mm.The intermediate portion has a length of between about 1.5 mm and about 5 mm.

La quantité de poudre gélifiante qui doit être incorporée dans le bouchon de la paillette avant la mise en oeuvre de l'invention dépend du type du bouchon et est connue de l'homme du métier.The amount of gelling powder that must be incorporated into the straw stopper before the implementation of the invention depends on the type of stopper and is known to those skilled in the art.

Dans tous les cas, il faut, selon l'invention, remplacer une partie de la poudre gélifiante par de la matière non absorbante sous forme dispersée.In all cases, according to the invention, it is necessary to replace part of the gelling powder with non-absorbent material in dispersed form.

La proportion de poudre gélifiante remplacée par de la matière non absorbante sous forme dispersée comprise entre environ 10% et environ 50% et dépend du type de bouchon. L'homme du métier déterminera aisément dans chaque cas la proportion de poudre gélifiante à remplacer par de la matière non absorbante sous forme dispersée pour obtenir une paillette avec un bouchon étanche et une absorption de liquide réduite.The proportion of gelling powder replaced by non-absorbent material in dispersed form between about 10% and about 50% and depends on the type of plug. Those skilled in the art will readily determine in each case the proportion of gelling powder to be replaced by non-absorbent material in dispersed form to obtain a straw with a sealed plug and reduced liquid absorption.

La paillette de l'invention présente une bonne étanchéité et une absorption très faible du liquide qui y est contenu, de l'ordre de 4 % et même moins.The straw of the invention has a good seal and a very low absorption of the liquid contained therein, of the order of 4% and even less.

L'invention sera décrite plus en détail mais de façon non limitative en référence aux Figures annexées dans lesquelles

  • la Figure 1 représente une vue partielle d'une paillette de l'invention selon le premier mode de réalisation,
  • la Figure 2 représente une vue partielle d'une paillette de l'invention selon le premier cas du deuxième mode de réalisation,
  • la Figure 3 représente une vue partielle d'une paillette de l'invention selon le deuxième cas du deuxième mode de réalisation,
  • la Figure 4 représente une vue partielle d'une paillette de l'invention selon le troisième cas du deuxième mode de réalisation,
  • la Figure 5 représente une vue partielle d'une paillette de l'invention selon le premier cas du troisième mode de réalisation,
  • la Figure 6 représente une vue partielle d'une paillette de l'invention selon le premier cas du troisième mode de réalisation.
The invention will be described in more detail but in a nonlimiting manner with reference to the appended figures in which
  • the Figure 1 represents a partial view of a straw of the invention according to the first embodiment,
  • the Figure 2 represents a partial view of a flake of the invention according to the first case of the second embodiment,
  • the Figure 3 represents a partial view of a flake of the invention according to the second case of the second embodiment,
  • the Figure 4 represents a partial view of a straw of the invention according to the third case of the second embodiment,
  • the Figure 5 represents a partial view of a flake of the invention according to the first case of the third embodiment,
  • the Figure 6 represents a partial view of a straw of the invention according to the first case of the third embodiment.

En référence à la Figure 1, la paillette 1 se compose d'un segment de tube 2 muni à l'une de ses extrémités d'un bouchon 3 tripartite.With reference to the Figure 1 , the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.

La partie externe 31, qui n'est pas en contact avec du liquide contenu dans la paillette, est une tresse en substance fibreuse.The outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.

La partie interne 33, en contact avec du liquide contenu dans la paillette, est une tresse en substance fibreuse.The inner portion 33, in contact with the liquid contained in the straw, is a braid of fibrous substance.

La partie intermédiaire 32 est constituée d'un mélange 4 de poudre gélifiante et de matière non absorbante sous forme dispersée.The intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.

En référence à la Figure 2, la paillette 1 se compose d'un segment de tube 2 muni à l'une de ses extrémités d'un bouchon 3 tripartite.With reference to the Figure 2 , the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.

La partie externe 31, qui n'est pas en contact avec du liquide contenu dans la paillette, est une tresse en substance fibreuse.The outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.

La partie interne 33, en contact avec du liquide contenu dans la paillette, est constituée d'un noyau 53 en matière thermoplastique gainé par des fils en une gaine 63.The inner part 33, in contact with the liquid contained in the straw, consists of a core 53 made of thermoplastic material sheathed by wires in a sheath 63.

La partie intermédiaire 32 est constituée d'un mélange 4 de poudre gélifiante et de matière non absorbante sous forme dispersée.The intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.

En référence à la Figure 3, la paillette 1 se compose d'un segment de tube 2 muni à l'une de ses extrémités d'un bouchon 3 tripartite.With reference to the Figure 3 , the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.

La partie externe 31, qui n'est pas en contact avec du liquide contenu dans la paillette, est une tresse en substance fibreuse.The outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.

La partie interne 33, en contact avec du liquide contenu dans la paillette, est constituée d'un noyau 53 en matière thermoplastique gainé par des fils en une gaine 63. Le noyau 53 comporte un alésage 73.The inner part 33, in contact with the liquid contained in the straw, consists of a core 53 of thermoplastic material sheathed by wires in a sheath 63. The core 53 has a bore 73.

La partie intermédiaire 32 est constituée d'un mélange 4 de poudre gélifiante et de matière non absorbante sous forme dispersée.The intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.

En référence à la Figure 4, la paillette 1 se compose d'un segment de tube 2 muni à l'une de ses extrémités d'un bouchon 3 tripartite.With reference to the Figure 4 , the straw 1 consists of a tube segment 2 provided at one of its ends with a tripartite plug 3.

La partie externe 31, qui n'est pas en contact avec du liquide contenu dans la paillette, est une tresse en substance fibreuse.The outer portion 31, which is not in contact with the liquid contained in the straw, is a braid of fibrous substance.

La partie interne 33, en contact avec du liquide contenu dans la paillette, est constituée d'un noyau 53 en matière thermoplastique gainé par des fils en une gaine 63. Le noyau 53 comporte une tresse centrale 83.The inner part 33, in contact with the liquid contained in the straw, consists of a core 53 of thermoplastic material sheathed by wires in a sheath 63. The core 53 comprises a central braid 83.

La partie intermédiaire 32 est constituée d'un mélange 4 de poudre gélifiante et de matière non absorbante sous forme dispersée.The intermediate portion 32 consists of a mixture 4 of gelling powder and non-absorbent material in dispersed form.

En référence à la Figure 5, la paillette 1 se compose d'un segment de tube 2 muni à l'une de ses extrémités d'un bouchon 3 monobloc.With reference to the Figure 5 , the straw 1 consists of a tube segment 2 provided at one of its ends with a plug 3 monobloc.

Le bouchon 3 est constitué d'un noyau 50 en matière thermoplastique gainé par des fils constituant une gaine 60. Les fils de la gaine 60 sont imprégnés d'un mélange 4 de poudre gélifiante et de matière non absorbante sous forme dispersée.The plug 3 consists of a core 50 of thermoplastic material sheathed by son forming a sheath 60. The son of the sheath 60 are impregnated with a mixture 4 of gelling powder and non-absorbent material in dispersed form.

En référence à la Figure 2, la paillette 1 se compose d'un segment de tube 2 muni à l'une de ses extrémités d'un bouchon 3 monobloc.With reference to the Figure 2 , the straw 1 consists of a tube segment 2 provided at one of its ends with a plug 3 monobloc.

Le bouchon 3 est constitué d'un noyau 50 en matière thermoplastique gainé par des fils constituant une gaine 60 comprenant une tresse centrale 80 en fils synthétiques imprégnée d'un mélange 4 de poudre gélifiante et de matière non absorbante sous forme dispersée.The plug 3 consists of a core 50 of thermoplastic material sheathed by son constituting a sheath 60 comprising a central braid 80 of synthetic son impregnated with a mixture 4 of gelling powder and non-absorbent material in dispersed form.

L'homme du métier comprendra que bien que l'invention ait été décrite et illustrée pour des modes de réalisation particuliers, de nombreuses variantes peuvent être envisagées tout en restant dans le cadre de l'invention tel que défini dans les revendications annexées.Those skilled in the art will understand that although the invention has been described and illustrated for particular embodiments, numerous variants can be envisaged while remaining within the scope of the invention as defined in the appended claims.

Claims (13)

  1. A straw for the conservation of small quantities of substances, in particular biological substances, comprising a segment of tube provided inside one end with a stopper including gel powder as well as a braid or a sheath, characterized in that a proportion of the gel powder is replaced with dispersed nonabsorbent material, said stopper thus comprising a mixture of gel powder and dispersed nonabsorbent material.
  2. A straw according to claim 1, characterized in that the gel powder is chosen from the group comprising alginates and polyvinyl alcohol.
  3. A straw according to either claim 1 or claim 2, characterized in that the nonabsorbent material is chosen from the group comprising glass, PVC, diatoms and perlites.
  4. A straw according to any of claims 1 to 3, characterized in that the nonabsorbent material takes the form of balls.
  5. A straw according to claim 4, characterized in that the average diameter of the balls is from approximately 50 µm to approximately 300 µm, for example.
  6. A straw according to any of claims 1 to 5, characterized in that the proportion of gel powder replaced with dispersed nonabsorbent material is from approximately 10% to approximately 50%.
  7. A straw according to any of claims 1 to 6, characterized in that the stopper is a three-part stopper comprising an external part that is not in direct contact with the liquid contained in said straw and an internal part that is in contact with the liquid contained in said straw, with between them an intermediate part containing the gel powder.
  8. A straw according to claim 7, characterized in that the stopper is a three-part stopper comprising a fibrous substance external part and a fibrous substance internal part with an intermediate part between them comprising a mixture of gel powder and dispersed nonabsorbent material.
  9. A straw according to claim 7, characterized in that the stopper comprises an external part comprising a fibrous substance braid, an internal part comprising a thermoplastics material core sheathed with filaments constituting a sheath, and an intermediate part comprising gel powder a proportion of which has been replaced with dispersed nonabsorbent material.
  10. A straw according to claim 7, characterized in that the three-part stopper comprises an external part comprising a fibrous substance braid, an internal part comprising a thermoplastics material core sheathed with filaments constituting a sheath, said core including a bore, and an intermediate part comprising gel powder a proportion of which has been replaced with dispersed nonabsorbent material.
  11. A straw according to claim 7, characterized in that the three-part stopper comprises an external part comprising a fibrous substance braid, an internal part comprising a thermoplastics material core sheathed with filaments constituting a sheath, said core including a central braid, and an intermediate part comprising gel powder a proportion of which has been replaced with dispersed nonabsorbent material.
  12. A straw according to any of claims 1 to 6, characterized in that the stopper is one-piece and comprises a thermoplastics material core sheathed with filaments constituting a sheath impregnated with a gel powder a proportion of which has been replaced with dispersed nonabsorbent material.
  13. A straw according to any of claims 1 to 6, characterized in that the stopper is one-piece and comprises a thermoplastics material core sheathed with filaments constituting a sheath with a central braid impregnated with gel powder a proportion of which has been replaced with dispersed nonabsorbent material.
EP02291040.0A 2001-05-07 2002-04-24 Straw for the preservation of small amounts of substances, in particular of biological liquids Expired - Lifetime EP1256328B8 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0106081 2001-05-07
FR0106081A FR2824256B1 (en) 2001-05-07 2001-05-07 STRAW FOR THE PRESERVATION OF SMALL QUANTITIES OF SUBSTANCES, ESPECIALLY BIOLOGICAL LIQUIDS

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EP1256328A1 EP1256328A1 (en) 2002-11-13
EP1256328B1 true EP1256328B1 (en) 2018-10-10
EP1256328B8 EP1256328B8 (en) 2018-12-26

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EP (1) EP1256328B8 (en)
JP (1) JP4257070B2 (en)
KR (1) KR20020086240A (en)
CN (1) CN1271983C (en)
CA (1) CA2382339C (en)
FR (1) FR2824256B1 (en)
MX (1) MXPA02004543A (en)
PL (1) PL200269B1 (en)
RU (1) RU2233756C2 (en)
TW (1) TWI255737B (en)

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EP2198804A1 (en) 2008-12-18 2010-06-23 IMV Technologies Straw for the conservation of a predetermined dose of a liquid base substance, in particular a biological substance
FR2955024B1 (en) * 2010-01-14 2012-02-10 Commissariat Energie Atomique DEVICE FOR TRANSIENTLY CONTACTING AT LEAST ONE CAPTURING UNIT OF BIOLOGICAL TARGETS WITH A FLUID CONTAINING THEM, AND METHOD OF RECOVERING TARGET TARGETS
FR3004332B1 (en) * 2013-04-11 2018-03-02 Imv Technologies STRAW FOR PRESERVING A PREDETERMINED DOSE OF A LIQUID-BASED SUBSTANCE, IN PARTICULAR PURE OR DILUTED ANIMAL SEED
FR3011731B1 (en) 2013-10-15 2016-10-28 Imv Tech (EN) STRAW FOR THE PRESERVATION OF A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCES, IN PARTICULAR PURE OR DILUTED ANIMAL SEED; AND TOGETHER THE COMPRISING
FR3011732B1 (en) * 2013-10-15 2016-09-09 Imv Tech (EN) STRAW FOR THE PRESERVATION OF A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCES, IN PARTICULAR PURE OR DILUTED ANIMAL SEED; AND TOGETHER THE COMPRISING
FR3017793B1 (en) 2014-02-21 2019-08-23 Imv Technologies PAILLETTE FOR PRESERVING A PREDETERMINED DOSE OF A LIQUID-BASED SUBSTANCE, IN PARTICULAR A BIOLOGICAL SUBSTANCE
FR3017792B1 (en) 2014-02-21 2019-08-23 Imv Technologies PAILLETTE FOR PRESERVING A PREDETERMINED DOSE OF A LIQUID-BASED SUBSTANCE, IN PARTICULAR A BIOLOGICAL SUBSTANCE
FR3021522A1 (en) 2014-05-28 2015-12-04 Imv Technologies STRAW FOR THE PRESERVATION OF A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCES, IN PARTICULAR DILUTED ANIMAL SEED; DILUTION MEDIUM FOR GIVING SUCH A SUBSTANCE; AND SYSTEM COMPRISING THEM
FR3034980B1 (en) 2015-04-20 2017-05-12 Imv Tech SPRAY FOR PRESERVING A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCE
FR3036954B1 (en) 2015-06-03 2017-07-14 Imv Tech STRAW FOR PRESERVING A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCE, AND INJECTION DEVICE AND METHOD FOR CARRYING OUT THE SAME
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CA2382339C (en) 2007-04-10
CN1271983C (en) 2006-08-30
PL200269B1 (en) 2008-12-31
EP1256328B8 (en) 2018-12-26
US7252988B2 (en) 2007-08-07
US20020188222A1 (en) 2002-12-12
TWI255737B (en) 2006-06-01
CN1383796A (en) 2002-12-11
PL353724A1 (en) 2002-11-18
JP2002345926A (en) 2002-12-03
KR20020086240A (en) 2002-11-18
MXPA02004543A (en) 2002-11-20
FR2824256A1 (en) 2002-11-08
RU2002111844A (en) 2004-01-20
FR2824256B1 (en) 2004-04-02
RU2233756C2 (en) 2004-08-10
EP1256328A1 (en) 2002-11-13
CA2382339A1 (en) 2002-11-07
JP4257070B2 (en) 2009-04-22

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