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EP1159070B1 - Gerät zum kapillaren flüssigkeitstransfer in seinem inneren - Google Patents

Gerät zum kapillaren flüssigkeitstransfer in seinem inneren Download PDF

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Publication number
EP1159070B1
EP1159070B1 EP00910911A EP00910911A EP1159070B1 EP 1159070 B1 EP1159070 B1 EP 1159070B1 EP 00910911 A EP00910911 A EP 00910911A EP 00910911 A EP00910911 A EP 00910911A EP 1159070 B1 EP1159070 B1 EP 1159070B1
Authority
EP
European Patent Office
Prior art keywords
groove
sample
deep groove
capillary action
partition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP00910911A
Other languages
English (en)
French (fr)
Other versions
EP1159070A1 (de
Inventor
Bruno Colin
Marie Privat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of EP1159070A1 publication Critical patent/EP1159070A1/de
Application granted granted Critical
Publication of EP1159070B1 publication Critical patent/EP1159070B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Definitions

  • the present invention relates to an apparatus which includes within it compartments delimited by a partition, creating a space for oriented movement and independently of at least one liquid sample. When there is at least two different liquid samples, it is possible to move them, to make them converge and make them react together.
  • Document GB-A -2,261,284 therefore proposes a transfer device fluids to perform diagnostic tests. This device uses channels made of porous material.
  • the capillary function is used due to the use of a porous material. This requires the insertion of this porous material and also to provide an impermeable material between two channels of porous material containing different liquids. This technique is therefore quite expensive to implement. artwork.
  • the patent US-A-5,842,787 relates to microfluidic systems which incorporate channels of variable dimensions. These channels have essentially depths which may vary. However, these variations are also associated with the width of these channels. Thus the greater the depth of a channel, the more its width is small, and vice versa.
  • the proposed apparatus solves all of the problems raised by proposing a structure which uses capillarity to allow moving liquids while minimizing retention phenomena. This allows perfectly efficient guidance even in the presence of a free space, which does not provide delimitation of the transferred liquid.
  • the width of each groove deep has a dimension that does not involve capillarity.
  • At least one groove superficial is adjacent to a deep groove.
  • At least one deep groove is adjacent to a surface groove.
  • a deep groove is positioned between two surface grooves.
  • the deep groove has one end, and the two grooves surface meet at this end to create a reaction zone.
  • the reaction zone is at a distance from the bulkhead or bulkhead film that involves capillary action.
  • the reaction zone is at a distance from the partition or partitioning film which does not involve capillary action.
  • Figure 1 shows an elevational view of the face of the apparatus having the compartment according to the invention.
  • FIG. 2 represents a partial cross-sectional view along A-A of the figure 1.
  • FIG. 3 represents a view identical to FIG. 2 in which a sample liquid is present.
  • FIG. 4 represents a view identical to FIGS. 2 and 3 in which two different liquid samples are present.
  • Figure 5 shows a sectional view identical to Figure 2, but from a second embodiment containing a liquid sample.
  • Figure 6 shows a sectional view identical to Figure 2, but of a third embodiment of the present invention, wherein a liquid sample is present.
  • the present invention relates to an apparatus 1 well represented on all of the Figures 2 to 6 in partial sectional view according to three different embodiments.
  • the present invention relates to an apparatus 1 well represented on all of the Figures 2 to 6 in partial sectional view according to three different embodiments.
  • Such an apparatus 1 can be used for the analysis of one or more samples different liquids in which one seeks to identify one or more analytes, according to all simple or complex analysis processes involving one or more different reagents depending on the chemical, physical or biological nature of the analyte (s) research.
  • the technical principles defined below are not limited to an analyte particular, the only requirement being that the analyte is distributed in the sample to be analyzed in suspension or in solution.
  • the analysis process implemented work can be done, in homogeneous or heterogeneous or mixed form.
  • ligand any biological species such as, for example, an antigen, an antigen fragment, a peptide, antibody, antibody fragment, hapten, nucleic acid, fragment of nucleic acid, a hormone, a vitamin.
  • An example of application of analysis techniques concern immunoassays, whatever their format, by analysis direct or by competition.
  • Another example of application relates to the detection and / or quantification of nucleic acids including all the necessary operations to this detection and / or quantification from any sample containing target nucleic acids.
  • the device 1 is in fact made up of a card whose two upper and lower faces are parallel to each other.
  • the two faces are flat but the upper face is the most interesting for the present invention.
  • the upper flat surface 2 of the device 1 has cavities which create compartments 3.
  • the compartments are partitioned from the flush surfaces of surface 2 by means of a partition or partitioning film 4.
  • This compartment 3, thus isolated, is in fact made up of different shapes.
  • This figure 2 corresponds to the sectional view partial according to A-A of Figure 1.
  • we notice that the two grooves surface 16 are parallel to each other along the groove deep 6.
  • the deep groove 6 has one end 7 where the two surface grooves 16 meet to create a reaction zone 8.
  • first liquid sample 5 at one of the surface grooves 16. This is the case in FIG. 3. It is also possible to isolate by plus a second liquid sample 15 at the other surface groove 16. This is the case of FIG. 4. In fact, so that the liquids 5 and 15 remain in position at the level surface grooves 16 and do not mix, it is necessary that the distance separating the bottom of the surface groove 16 relative to the partitioning film 4 or low enough to involve the capillary force.
  • the right distance between the film 4 and the groove 16 to have an optimal capillary force is included between 50 and 800 micrometers ( ⁇ m), and preferably between 300 and 500 ⁇ m.
  • the distance between the film 4 and the groove 16 is chosen at 400 ⁇ m. This dimension is actually characteristic of liquids 5 and / or 15 which are used in the apparatus 1 in connection with the nature of the materials used in the device.
  • the distance separating the film 4 from the bottom of the deep groove 6 is very important so that no capillary force allows the retention of like for example the partitioning film or the card, it will be necessary to make possibly vary this distance.
  • the distance separating the film 4 from the bottom of the deep groove 6 is very important so that no capillary force allows the retention of liquid 5 or 15 at this level. It is of course obvious that it is necessary, that level of the width of this deep groove, there is no possibility of making intervene capillary action.
  • the nature of the flexible film may vary depending on the nature of the card analysis and fluids tested in particular for compatibility reasons.
  • a polymer film TPX polymethylpentene
  • BOPP bi-oriented polypropylene
  • the fixation of these films can be produced by gluing (coating of glue such as silicone glues on the film) or by welding.
  • An example of adhesive BOPP is provided by the company BioMérieux Inc (St Louis, MO, USA) under the reference 022004-2184.
  • the analysis card is obtained by machining a material technical plastic such as impact polystyrene reference R540E from the GOODFELLOW company, compatible with treated liquids.
  • the card could be obtained by precision molding, but all other manufacturing methods and in particular those used in the techniques of semiconductor like those described in patent application WO-A-97/02357 can be used to manufacture the analysis card.
  • Figure 5 it is essentially a reverse construction to the first embodiment of Figures 1 to 4.
  • the surface groove 16 is located in the center and is surrounded by two deep grooves 6. The liquid sample 5 is then only in contact with the bottom of the surface groove 16.
  • FIG. 6 it is possible according to FIG. 6 not to have only one surface groove 16 and only one deep groove 6.
  • the movement of liquids 5 and 15 is carried out in different ways. Through example, we can generate vibrations; we can position the card 1 in a substantially vertical position, in which liquids have their movement which is eased by gravity; you can use centrifugal force.
  • Pumping systems can be incorporated inside or outside the device, for example diaphragm pumps (US-A-5,277,556), piezoelectric peristaltic pumps (US-A-5,126,022), ferrofluid transport systems, pumps electrical and hydrodynamic (Richter et al., Sensors and Actuators, 29, p159-165, 1991). It is also possible to use the combination of at least two of these techniques.

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  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • External Artificial Organs (AREA)

Claims (8)

  1. Eine Vorrichtung (1), die mindestens eine ebene Fläche (2), auf der sich Kammern (3) befinden und durch eine Zwischenwand (4) begrenzt sind, beinhaltet, wobei die Kammern einen Zwischenraum schaffen, der das Verdrängen einer flüssigen Probe (5 oder 15) oder das unabhängige Verdrängen von mindestens zwei flüssigen Proben (5 und 15) ermöglicht, wobei die Kammern (3) aus mindestens zwei unterschiedlichen Rillenarten bestehen:
    eine erste Art von Rille(n), die tief (6) ist/sind und als ein Aufteilungsmittel der Probe(n) (5 und/oder 15) dient/dienen, wobei sich die tiefe(n) Rille(n) (6) in einer Entfernung von der Zwischenwand (4) befindet/befinden, die die Kapillarität nicht hervorruft, und
    eine zweite Art von Rille(n), die oberflächlich (16) ist/sind und als ein Empfangsmittel der Probe(n) (5 und/oder 15) dient/dienen, wobei sich die oberflächliche(n) Rille(n) (16) in einer Entfernung von der Zwischenwand (4) befindet/befinden, die die Kapillarität hervorruft,
    wobei die zwei Rillenarten (6 und 16) das Führen der Verdrängungen der Probe(n) (5 und/oder 15) je nach Ausrichtung der Vorrichtung (1) erlauben.
  2. Vorrichtung gemäß Anspruch 1, dadurch gekennzeichnet, dass die Breite jeder tiefen Rille (6) so bemessen ist, dass sie die Kapillarität nicht hervorruft.
  3. Vorrichtung gemäß Anspruch 1 oder 2, dadurch gekennzeichnet, dass mindestens eine oberflächliche Rille (16) angrenzend an eine tiefe Rille (6) angeordnet ist.
  4. Vorrichtung gemäß einem der Ansprüche 1 bis 3, dadurch gekennzeichnet, dass mindestens eine tiefe Rille (6) angrenzend an eine oberflächliche Rille (16) angeordnet ist.
  5. Vorrichtung gemäß einem der Ansprüche 1 bis 4, dadurch gekennzeichnet, dass mindestens eine tiefe Rille (6) zwischen zwei oberflächlichen Rillen (16) positioniert ist.
  6. Vorrichtung gemäß Anspruch 5, dadurch gekennzeichnet, dass die tiefe Rille (6) einen freien Endpunkt (7) beinhaltet und dass sich die zwei oberflächlichen Rillen (16) an diesem freien Endpunkt treffen, um einen Reaktionsbereich (8) zu schaffen, wo mindestens zwei flüssige Proben (5 und 15), die eine (5) und die andere (15), zusammengeführt werden, und diese möglicherweise miteinander reagieren.
  7. Vorrichtung gemäß Anspruch 6, dadurch gekennzeichnet, dass sich der Reaktionsbereich (8) in einer Entfernung von der Zwischenwand (4) befindet, die die Kapillarität hervorruft.
  8. Vorrichtung gemäß Anspruch 6, dadurch gekennzeichnet, dass sich der Reaktionsbereich in einer Entfernung von der Zwischenwand (4) befindet, die die Kapillarität nicht hervorruft.
EP00910911A 1999-03-09 2000-03-09 Gerät zum kapillaren flüssigkeitstransfer in seinem inneren Expired - Lifetime EP1159070B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9903034A FR2790684B1 (fr) 1999-03-09 1999-03-09 Appareil permettant en son sein le transfert de liquides par capillarite
FR9903034 1999-03-09
PCT/FR2000/000581 WO2000053321A1 (fr) 1999-03-09 2000-03-09 Appareil permettant en son sein le transfert de liquides par capillarite

Publications (2)

Publication Number Publication Date
EP1159070A1 EP1159070A1 (de) 2001-12-05
EP1159070B1 true EP1159070B1 (de) 2003-12-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP00910911A Expired - Lifetime EP1159070B1 (de) 1999-03-09 2000-03-09 Gerät zum kapillaren flüssigkeitstransfer in seinem inneren

Country Status (10)

Country Link
US (1) US7169353B1 (de)
EP (1) EP1159070B1 (de)
JP (1) JP4360454B2 (de)
AT (1) ATE256499T1 (de)
AU (1) AU761808B2 (de)
CA (1) CA2362412C (de)
DE (1) DE60007285T2 (de)
ES (1) ES2212990T3 (de)
FR (1) FR2790684B1 (de)
WO (1) WO2000053321A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1716404A1 (de) * 2004-02-20 2006-11-02 The Research Foundation Of State University Of New York Verfahren und vorrichtung zur manipulation von flüssigkeiten in mikrofluidsystemen

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1716404A1 (de) * 2004-02-20 2006-11-02 The Research Foundation Of State University Of New York Verfahren und vorrichtung zur manipulation von flüssigkeiten in mikrofluidsystemen
EP1716404A4 (de) * 2004-02-20 2010-05-05 Univ New York State Res Found Verfahren und vorrichtung zur manipulation von flüssigkeiten in mikrofluidsystemen

Also Published As

Publication number Publication date
US7169353B1 (en) 2007-01-30
CA2362412A1 (fr) 2000-09-14
WO2000053321A1 (fr) 2000-09-14
FR2790684B1 (fr) 2001-05-11
EP1159070A1 (de) 2001-12-05
DE60007285T2 (de) 2004-09-02
CA2362412C (en) 2008-08-26
DE60007285D1 (de) 2004-01-29
JP4360454B2 (ja) 2009-11-11
JP2002538482A (ja) 2002-11-12
AU761808B2 (en) 2003-06-12
ATE256499T1 (de) 2004-01-15
AU3295300A (en) 2000-09-28
ES2212990T3 (es) 2004-08-16
FR2790684A1 (fr) 2000-09-15

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