EP1021557A1 - Dispositif de surveillance microbienne - Google Patents
Dispositif de surveillance microbienneInfo
- Publication number
- EP1021557A1 EP1021557A1 EP97943349A EP97943349A EP1021557A1 EP 1021557 A1 EP1021557 A1 EP 1021557A1 EP 97943349 A EP97943349 A EP 97943349A EP 97943349 A EP97943349 A EP 97943349A EP 1021557 A1 EP1021557 A1 EP 1021557A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- fluorescent
- fluorescent compound
- compound
- matrix
- respiring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000000813 microbial effect Effects 0.000 title description 11
- 238000012806 monitoring device Methods 0.000 title description 3
- 244000005700 microbiome Species 0.000 claims abstract description 70
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 67
- 239000001301 oxygen Substances 0.000 claims abstract description 67
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 67
- 238000000034 method Methods 0.000 claims abstract description 61
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 44
- 150000001875 compounds Chemical class 0.000 claims abstract description 35
- 239000000203 mixture Substances 0.000 claims abstract description 34
- 230000008859 change Effects 0.000 claims abstract description 23
- 239000012530 fluid Substances 0.000 claims abstract description 21
- 238000005259 measurement Methods 0.000 claims abstract description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 12
- 230000009467 reduction Effects 0.000 claims abstract description 5
- 230000001678 irradiating effect Effects 0.000 claims abstract 10
- 230000003115 biocidal effect Effects 0.000 claims description 24
- 230000000694 effects Effects 0.000 claims description 21
- 239000011159 matrix material Substances 0.000 claims description 21
- 229920001296 polysiloxane Polymers 0.000 claims description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000003242 anti bacterial agent Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000004033 plastic Substances 0.000 claims description 8
- 229920001971 elastomer Polymers 0.000 claims description 7
- 239000005060 rubber Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 6
- 230000001066 destructive effect Effects 0.000 claims description 5
- 229920002379 silicone rubber Polymers 0.000 claims description 5
- FCNCGHJSNVOIKE-UHFFFAOYSA-N 9,10-diphenylanthracene Chemical compound C1=CC=CC=C1C(C1=CC=CC=C11)=C(C=CC=C2)C2=C1C1=CC=CC=C1 FCNCGHJSNVOIKE-UHFFFAOYSA-N 0.000 claims description 4
- VOAPTKOANCCNFV-UHFFFAOYSA-N hexahydrate;hydrochloride Chemical compound O.O.O.O.O.O.Cl VOAPTKOANCCNFV-UHFFFAOYSA-N 0.000 claims description 4
- 229910052707 ruthenium Inorganic materials 0.000 claims description 4
- 239000004945 silicone rubber Substances 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 3
- WHELTKFSBJNBMQ-UHFFFAOYSA-L dichlororuthenium;2-pyridin-2-ylpyridine;hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].[Cl-].[Ru+2].N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1 WHELTKFSBJNBMQ-UHFFFAOYSA-L 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- -1 tris-2, 2 -bipyridyl ruthenium (II) salt Chemical compound 0.000 claims description 2
- 230000000845 anti-microbial effect Effects 0.000 claims 11
- 230000035699 permeability Effects 0.000 claims 4
- 229940068911 chloride hexahydrate Drugs 0.000 claims 3
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 claims 1
- 239000000523 sample Substances 0.000 description 37
- 238000012360 testing method Methods 0.000 description 34
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 22
- 239000000725 suspension Substances 0.000 description 21
- 241000588724 Escherichia coli Species 0.000 description 18
- 229960001668 cefuroxime Drugs 0.000 description 15
- JFPVXVDWJQMJEE-IZRZKJBUSA-N cefuroxime Chemical compound N([C@@H]1C(N2C(=C(COC(N)=O)CS[C@@H]21)C(O)=O)=O)C(=O)\C(=N/OC)C1=CC=CO1 JFPVXVDWJQMJEE-IZRZKJBUSA-N 0.000 description 15
- GNWUOVJNSFPWDD-XMZRARIVSA-M Cefoxitin sodium Chemical compound [Na+].N([C@]1(OC)C(N2C(=C(COC(N)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)CC1=CC=CS1 GNWUOVJNSFPWDD-XMZRARIVSA-M 0.000 description 13
- 229960002682 cefoxitin Drugs 0.000 description 13
- 229940088710 antibiotic agent Drugs 0.000 description 12
- 238000001514 detection method Methods 0.000 description 12
- 239000000243 solution Substances 0.000 description 12
- 230000003377 anti-microbal effect Effects 0.000 description 11
- 229960003405 ciprofloxacin Drugs 0.000 description 11
- 230000036284 oxygen consumption Effects 0.000 description 11
- 239000012528 membrane Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 230000002503 metabolic effect Effects 0.000 description 8
- 239000000741 silica gel Substances 0.000 description 8
- 229910002027 silica gel Inorganic materials 0.000 description 8
- 230000000007 visual effect Effects 0.000 description 8
- 239000012085 test solution Substances 0.000 description 7
- OURODNXVJUWPMZ-UHFFFAOYSA-N 1,2-diphenylanthracene Chemical compound C1=CC=CC=C1C1=CC=C(C=C2C(C=CC=C2)=C2)C2=C1C1=CC=CC=C1 OURODNXVJUWPMZ-UHFFFAOYSA-N 0.000 description 6
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical compound N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 6
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- 230000005284 excitation Effects 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 238000012544 monitoring process Methods 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 238000010791 quenching Methods 0.000 description 6
- 239000001974 tryptic soy broth Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 229920004482 WACKER® Polymers 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 239000002480 mineral oil Substances 0.000 description 5
- 235000010446 mineral oil Nutrition 0.000 description 5
- 230000000171 quenching effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000003638 chemical reducing agent Substances 0.000 description 4
- 229910000365 copper sulfate Inorganic materials 0.000 description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000186365 Mycobacterium fortuitum Species 0.000 description 3
- 230000003466 anti-cipated effect Effects 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 230000008020 evaporation Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 238000005497 microtitration Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 201000008827 tuberculosis Diseases 0.000 description 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 238000009635 antibiotic susceptibility testing Methods 0.000 description 2
- 239000012298 atmosphere Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- RGVBVVVFSXWUIM-UHFFFAOYSA-M bromo(dimethyl)sulfanium;bromide Chemical compound [Br-].C[S+](C)Br RGVBVVVFSXWUIM-UHFFFAOYSA-M 0.000 description 2
- 229910001882 dioxygen Inorganic materials 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003269 fluorescent indicator Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 235000010265 sodium sulphite Nutrition 0.000 description 2
- 238000011179 visual inspection Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920002799 BoPET Polymers 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 239000005041 Mylar™ Substances 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000003848 UV Light-Curing Methods 0.000 description 1
- KGTSLTYUUFWZNW-PPJQWWMSSA-N [(7S,9E,11S,12R,13S,14R,15R,16R,17S,18S,19E,21Z)-2,15,17,27,29-pentahydroxy-11-methoxy-3,7,12,14,16,18,22-heptamethyl-26-[(E)-(4-methylpiperazin-1-yl)iminomethyl]-6,23-dioxo-8,30-dioxa-24-azatetracyclo[23.3.1.14,7.05,28]triaconta-1(29),2,4,9,19,21,25,27-octaen-13-yl] acetate pyridine-4-carbohydrazide Chemical compound NNC(=O)c1ccncc1.CO[C@H]1\C=C\O[C@@]2(C)Oc3c(C2=O)c2c(O)c(\C=N\N4CCN(C)CC4)c(NC(=O)\C(C)=C/C=C/[C@H](C)[C@H](O)[C@@H](C)[C@@H](O)[C@@H](C)[C@H](OC(C)=O)[C@@H]1C)c(O)c2c(O)c3C KGTSLTYUUFWZNW-PPJQWWMSSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000001355 anti-mycobacterial effect Effects 0.000 description 1
- 239000003926 antimycobacterial agent Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 238000002815 broth microdilution Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001723 curing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- DHCWLIOIJZJFJE-UHFFFAOYSA-L dichlororuthenium Chemical compound Cl[Ru]Cl DHCWLIOIJZJFJE-UHFFFAOYSA-L 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000000890 drug combination Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000004941 influx Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 229920002631 room-temperature vulcanizate silicone Polymers 0.000 description 1
- YAYGSLOSTXKUBW-UHFFFAOYSA-N ruthenium(2+) Chemical class [Ru+2] YAYGSLOSTXKUBW-UHFFFAOYSA-N 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000002076 α-tocopherol Substances 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
- 235000004835 α-tocopherol Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/40—Detection of gases
- C12Q2304/44—Oxygen
Definitions
- the processes of the instant invention use a fluorescence detection system wherein the fluorescing sensor compound is one which exhibits a quantifiable degree of quenching when exposed to oxygen.
- the sensor compound may be brought into contact with the test sample (either directly or separated by an oxygen permeable membrane) and the fluorescence is measured or observed visually with appropriate aids.
- an increase in fluorescence is indicative of respiring aerobic microorganisms, which utilize (and thereby reduce) t thhee o oxxvygeeenn i inn t thhee s saammopllee
- Figure 2 graphically depicts the intensity of fluorescence as a function of time for indicators in contact with broth inoculated with different concentrations of microorganisms.
- Figure 4 graphically depicts the intensity of fluorescence as a function of time for indicators in contact with broth inoculated with the same number of organisms but containing different amounts of copper sulfate.
- the system can be allowed to interact unobserved for a predetermined amount of time after which the presence or absence of fluorescence is observed and compared to appropnate control samples, yielding results that are often obtained with a smgle such observation
- a particular benefit of this system is that the measurement of fluorescence is non-destructive and if after a penod of time (e g 4 hours) the results are non-conclusive, the system can be re-incubated and read aga at a later tune
- reagent controls such is by no means necessary, and it is postulated that, by appropnate choice of fluorescent compounds, a skilled technician or technologist would be capable of mdependently determining whether the results mdicate the , presence of microbial activity
- the fluorophore can also be in a liquid phase separated from the solution being analyzed by a membrane that is impermeable to the indicator molecules and to microorganisms in the sample but which is permeable to oxygen. Additionally, less- sensitive sensors can be fabricated by using less 0-2 permeable polymers or by using compounds with shorter excited-state lifetimes.
- the wells containing the highest concentrations of reducing agent have the highest fluorescence intensity, thus demonstrating the relationship between O2 concentration and fluorescence.
- EXAMPLE 10 Effect of Antibiotics on the Oxygen Consumption of Microorganisms Using DPA Fluorescence Indicator
- a 1 x 10 7 CFU/mL suspension of E. coli ATCC #25922 in Mueller Hinton broth was prepared; 150 microliter aliquots were pipetted into the odd numbered rows of a microtiter tray, while 150 microliter aliquots of uninoculated Mueller Hinton broth were pipetted into the wells of the even numbered columns.
- the lids containing the indicator coated prongs were placed on the trays.
- the lidded trays were placed in a 37°C high humidity incubator for 3 hours.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
La présente invention concerne un procédé de détection de la présence de micro-organismes respirants dans un fluide. Ledit procédé se déroule de la façon suivante: (i) placer le fluide dans un récipient dans lequel il sera sensiblement isolé de l'oxygène de l'air. Un détecteur sera ensuite placé à l'intérieur dudit récipient, sans qu'il soit en contact direct avec le fluide, la composition de ce détecteur consistant en un composé fluorescent présentant une intensité fluorescente réduite lorsqu'il est soumis à un rayonnement à des longueurs d'ondes de lumière; (ii) soumettre à un rayonnement cette composition de détecteur avec des longueurs d'ondes de lumière de façon à ce que ledit composé fluorescent émette une fluorescence; (iii) mesurer ou observer visuellement l'intensité lumineuse fluorescente provenant de ce composé fluorescent tout en soumettant le composé du détecteur à un rayonnement avec ladite lumière; (iv) comparer cette mesure avec celle effectuée sur un témoin exempt de micro-organismes respirants, ce témoin étant tiré du groupe composé d'un témoin réactif n'étant pas en contact avec des micro-organismes respirants et d'un seuil calculé, dans lequel le changement de l'intensité fluorescente par rapport à l'intensité fluorescente du témoin indique la présence de micro-organismes respirants; et (v) au cas où aucune augmentation n'a été mesurée ou observée, répéter, selon les besoins, les étapes (ii), (iii) et (iv), afin de détecter la présence de micro-organismes respirants dans ledit fluide.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71555796A | 1996-09-18 | 1996-09-18 | |
| US715557 | 1996-09-18 | ||
| PCT/US1997/016496 WO1998012348A1 (fr) | 1996-09-18 | 1997-09-18 | Dispositif de surveillance microbienne |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1021557A1 true EP1021557A1 (fr) | 2000-07-26 |
Family
ID=24874543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP97943349A Withdrawn EP1021557A1 (fr) | 1996-09-18 | 1997-09-18 | Dispositif de surveillance microbienne |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1021557A1 (fr) |
| JP (1) | JP2002501363A (fr) |
| AU (1) | AU4483997A (fr) |
| CA (1) | CA2264272A1 (fr) |
| WO (1) | WO1998012348A1 (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6395506B1 (en) * | 1991-04-18 | 2002-05-28 | Becton, Dickinson And Company | Device for monitoring cells |
| US5998517A (en) * | 1998-06-05 | 1999-12-07 | Becton, Dickinson And Company | Composition for the detection of microorganisms in a sample |
| US6432697B1 (en) * | 2000-02-03 | 2002-08-13 | Becton, Dickinson And Company | Transparent sample container |
| EP1134583A1 (fr) * | 2000-03-17 | 2001-09-19 | Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO | Mesure de changements du métabolisme |
| US20050059140A1 (en) * | 2003-09-12 | 2005-03-17 | Andrea Liebmann-Vinson | Methods of surface modification to enhance cell adhesion |
| EP2250281B1 (fr) * | 2008-02-19 | 2015-04-08 | Becton Dickinson and Company | Systèmes et procédés d'identification présumée de type de micro-organismes dans une culture |
| US10337046B2 (en) | 2008-02-19 | 2019-07-02 | Becton, Dickinson And Company | High confidence in positive status determination |
| US8613158B2 (en) | 2008-04-18 | 2013-12-24 | Ball Horticultural Company | Method for grouping a plurality of growth-induced seeds for commercial use or sale based on testing of each individual seed |
| JP5901689B2 (ja) * | 2014-05-07 | 2016-04-13 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | 培養物中の微生物の種類の仮同定のためのシステムおよび方法 |
| RU2576030C1 (ru) * | 2015-03-04 | 2016-02-27 | Сергей Дмитриевич Иванов | Способ определения опасности микробиологической загрязненности воды |
| WO2018213275A1 (fr) | 2017-05-16 | 2018-11-22 | Agilent Technologies, Inc. | Couvercle de plaque de microtitration éliminant le vide et procédé de mesure optique de la concentration d'oxygène de puits à travers le couvercle |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1261717A (fr) * | 1982-12-23 | 1989-09-26 | John R. Bacon | Methode et appareil pour mesurer l'oxygene |
| US4945060A (en) * | 1988-03-15 | 1990-07-31 | Akzo N. V. | Device for detecting microorganisms |
| EP0448923B1 (fr) * | 1990-03-29 | 1996-01-03 | Avl Photronics Corporation | Méthode et dispositif pour la détection d'activités biologiques dans un échantillon |
| AU647609B2 (en) * | 1991-04-18 | 1994-03-24 | Becton Dickinson & Company | Microbial monitoring device |
-
1997
- 1997-09-18 WO PCT/US1997/016496 patent/WO1998012348A1/fr not_active Application Discontinuation
- 1997-09-18 EP EP97943349A patent/EP1021557A1/fr not_active Withdrawn
- 1997-09-18 JP JP51484198A patent/JP2002501363A/ja not_active Ceased
- 1997-09-18 CA CA002264272A patent/CA2264272A1/fr not_active Abandoned
- 1997-09-18 AU AU44839/97A patent/AU4483997A/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO9812348A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002501363A (ja) | 2002-01-15 |
| AU4483997A (en) | 1998-04-14 |
| WO1998012348A1 (fr) | 1998-03-26 |
| CA2264272A1 (fr) | 1998-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2066329C (fr) | Appareil de surveillance des microbes | |
| US6900030B2 (en) | Device for monitoring cells | |
| US5164301A (en) | Process and kit for detecting microbial metabolism | |
| US5064756A (en) | Microbial susceptibility assay using 7-n-(aminoacyl)-7-amido-4-methylcoumarins | |
| DE68923720T2 (de) | Einrichtung und Gerät für die Detektion von Mikroorganismen. | |
| DE69127387T2 (de) | Vorrichtung und Verfahren zum Nachweis von Mikroorganismen | |
| Thom et al. | Factors affecting the selection and use of tetrazolium salts as cytochemical indicators of microbial viability and activity | |
| US5217876A (en) | Method for detecting microorganisms | |
| US6989246B2 (en) | Sensor formulation for simultaneously monitoring at least two components of a gas composition | |
| US5079144A (en) | Microorganism testing with a hydrolyzable fluorogenic substrate | |
| JPH11510051A (ja) | 複数ゾーン滅菌インジケーター | |
| WO1998012348A1 (fr) | Dispositif de surveillance microbienne | |
| WO1998012348A9 (fr) | Dispositif de surveillance microbienne | |
| Brouwer | Testing for chemical toxicity using bacteria: An undergraduate laboratory experiment | |
| US6509166B1 (en) | Detection and quantification of one or more target analytes in a sample using spatially localized analyte reproduction | |
| US4340671A (en) | E. coli sensitivity broth | |
| JP2002500020A (ja) | 嫌気性細菌の毒性化合物による阻止および殺害を迅速に評価する方法 | |
| Badalyan | Sensor platform for rapid detection and quantification of indicator and pathogenic bacteria on food contact surfaces | |
| RU2175352C1 (ru) | Способ определения концентрации инсектицида метафоса и продукта гидролиза фосфорорганических нитроароматических инсектицидов пара-нитрофенола в водной среде | |
| EP0877093A1 (fr) | Milieu pour une culture sanguine et méthode pour la détection et pour l'identification de micro-organismes | |
| Rogers et al. | Identification of bacteria by rapid spectrophotofluorometric methods | |
| WO2001090402A1 (fr) | Nouveau procede et nouveau dispositif |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 19990413 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): DE FR GB IT |
|
| 17Q | First examination report despatched |
Effective date: 20010606 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
| 18D | Application deemed to be withdrawn |
Effective date: 20011017 |