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EP0446272A4 - Expression of hiv proteins in -i(drosophila) cells - Google Patents

Expression of hiv proteins in -i(drosophila) cells

Info

Publication number
EP0446272A4
EP0446272A4 EP19900900559 EP90900559A EP0446272A4 EP 0446272 A4 EP0446272 A4 EP 0446272A4 EP 19900900559 EP19900900559 EP 19900900559 EP 90900559 A EP90900559 A EP 90900559A EP 0446272 A4 EP0446272 A4 EP 0446272A4
Authority
EP
European Patent Office
Prior art keywords
drosophila
expression
cells
hiv proteins
hiv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19900900559
Other languages
English (en)
Other versions
EP0446272A1 (en
Inventor
Hanne Ranch Johansen
Martin Rosenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of EP0446272A1 publication Critical patent/EP0446272A1/en
Publication of EP0446272A4 publication Critical patent/EP0446272A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • AIDS & HIV (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
EP19900900559 1988-12-01 1989-11-29 Expression of hiv proteins in -i(drosophila) cells Withdrawn EP0446272A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27838688A 1988-12-01 1988-12-01
US278386 1988-12-01

Publications (2)

Publication Number Publication Date
EP0446272A1 EP0446272A1 (en) 1991-09-18
EP0446272A4 true EP0446272A4 (en) 1993-01-07

Family

ID=23064787

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19900900559 Withdrawn EP0446272A4 (en) 1988-12-01 1989-11-29 Expression of hiv proteins in -i(drosophila) cells

Country Status (12)

Country Link
EP (1) EP0446272A4 (da)
JP (1) JP3085704B2 (da)
KR (1) KR0152525B1 (da)
AU (1) AU630649B2 (da)
CA (1) CA2003794C (da)
DK (1) DK175461B1 (da)
FI (1) FI113475B (da)
IL (1) IL92470A (da)
NO (1) NO314090B1 (da)
PT (1) PT92477A (da)
WO (1) WO1990006358A1 (da)
ZA (1) ZA899150B (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2841376C (en) * 2011-07-05 2023-02-28 Duke University N-terminal deleted gp120 immunogens
WO2013052095A2 (en) 2011-10-03 2013-04-11 Duke University Vaccine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290261A1 (en) * 1987-05-08 1988-11-09 Smithkline Beecham Corporation Expression of foreign genes in drosophila cells
WO1990007006A1 (en) * 1988-12-21 1990-06-28 Smithkline Biologicals Expression of the plasmodium circumsporozoite protein in insect cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0272858A3 (en) * 1986-12-15 1989-07-12 Repligen Corporation Recombinant hiv envelope proteins produced in insect cells
AP129A (en) * 1988-06-03 1991-04-17 Smithkline Biologicals S A Expression of retrovirus gag protein eukaryotic cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290261A1 (en) * 1987-05-08 1988-11-09 Smithkline Beecham Corporation Expression of foreign genes in drosophila cells
WO1990007006A1 (en) * 1988-12-21 1990-06-28 Smithkline Biologicals Expression of the plasmodium circumsporozoite protein in insect cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
5TH CONFERENCE INTERNATIONALE SUR LE SIDA, Montreal, Quebec, 1st - 9th June 1989 (INTERNATIONAL DEVELOPMENT CENTRE, Ottawa, Ontario, Illus. Paper, 1989, MOISSER ED.), page 584, abstract no. T.C.P. 106; H. JOHANSEN et al.: "Stable expression and secretion of recombinant HIV 1 envelope protein in Drosophila Schneider cells" *
NUCLEIC ACIDS RESEARCH, vol. 16, no. 3, 2nd February 1988, pages 1043-1061; T.A. BUNCH et al.: "Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells" *
See also references of WO9006358A1 *

Also Published As

Publication number Publication date
EP0446272A1 (en) 1991-09-18
AU4755290A (en) 1990-06-26
CA2003794A1 (en) 1990-06-01
WO1990006358A1 (en) 1990-06-14
PT92477A (pt) 1990-06-29
NO912099D0 (no) 1991-05-31
AU630649B2 (en) 1992-11-05
CA2003794C (en) 2000-02-08
KR910700344A (ko) 1991-03-14
NO314090B1 (no) 2003-01-27
DK175461B1 (da) 2004-11-01
FI113475B (fi) 2004-04-30
IL92470A0 (en) 1990-08-31
IL92470A (en) 1997-01-10
KR0152525B1 (ko) 1998-10-01
NO912099L (no) 1991-07-31
JPH04501954A (ja) 1992-04-09
DK104991A (da) 1991-05-31
DK104991D0 (da) 1991-05-31
JP3085704B2 (ja) 2000-09-11
FI912635A0 (fi) 1991-05-31
ZA899150B (en) 1991-04-24

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Legal Events

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Effective date: 19940607