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EP0264753A3 - Method for determining plasminogen - Google Patents

Method for determining plasminogen Download PDF

Info

Publication number
EP0264753A3
EP0264753A3 EP87114820A EP87114820A EP0264753A3 EP 0264753 A3 EP0264753 A3 EP 0264753A3 EP 87114820 A EP87114820 A EP 87114820A EP 87114820 A EP87114820 A EP 87114820A EP 0264753 A3 EP0264753 A3 EP 0264753A3
Authority
EP
European Patent Office
Prior art keywords
plasminogen
streptokinase
plasmin
fragment
rate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP87114820A
Other languages
German (de)
Other versions
EP0264753B1 (en
EP0264753A2 (en
Inventor
Hans-Jurgen Dr. Kolde
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Priority to AT87114820T priority Critical patent/ATE85364T1/en
Publication of EP0264753A2 publication Critical patent/EP0264753A2/en
Publication of EP0264753A3 publication Critical patent/EP0264753A3/en
Application granted granted Critical
Publication of EP0264753B1 publication Critical patent/EP0264753B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci
    • G01N2333/3153—Streptokinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/968—Plasmin, i.e. fibrinolysin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for determining plasminogen in body fluids in the presence of streptokinase using a substrate for plasmin, from which an optically measurable fragment is produced by the action of plasmin, is characterised in that the body fluid is mixed essentially simultaneously with streptokinase and a chromogenic or fluorogenic substrate for plasmin, and the amount of the fragment produced in a particular time, or the rate of formation of the fragment, and from this the concentration of plasminogen, is determined. When the plasminogen present is in molar excess compared with streptokinase this method can be used to determine the rate of activation of plasminogen.
EP87114820A 1986-10-16 1987-10-10 Method for determining plasminogen Expired - Lifetime EP0264753B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT87114820T ATE85364T1 (en) 1986-10-16 1987-10-10 PROCEDURE FOR DETERMINING PLASMINOGEN.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3635191 1986-10-16
DE19863635191 DE3635191A1 (en) 1986-10-16 1986-10-16 METHOD FOR DETERMINING PLASMINOGENS

Publications (3)

Publication Number Publication Date
EP0264753A2 EP0264753A2 (en) 1988-04-27
EP0264753A3 true EP0264753A3 (en) 1988-12-28
EP0264753B1 EP0264753B1 (en) 1993-02-03

Family

ID=6311820

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87114820A Expired - Lifetime EP0264753B1 (en) 1986-10-16 1987-10-10 Method for determining plasminogen

Country Status (7)

Country Link
EP (1) EP0264753B1 (en)
JP (1) JP2540563B2 (en)
AT (1) ATE85364T1 (en)
AU (1) AU602535B2 (en)
CA (1) CA1316088C (en)
DE (2) DE3635191A1 (en)
ES (1) ES2053496T3 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3838529A1 (en) * 1988-11-14 1990-05-17 Behringwerke Ag GLOBALTEST FOR DETECTING THE MAIN COMPONENTS OF THE FIBRINOLYSIS SYSTEM
DE19914811A1 (en) 1999-03-31 2000-10-05 Henkel Kgaa Detergent compositions containing a bleaching agent include a combination of a cyanomethyl ammonium salt bleach activator and an enzyme
FR2979635B1 (en) 2011-09-07 2015-03-20 Hyphen Biomed PROCESS FOR DETERMINING PLASMINOGEN IN A LIQUID MEDIUM, COMPOSITIONS AND KIT THEREFOR.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2521206A1 (en) * 1975-05-13 1976-12-02 Eckart Dr Med Jacobi Plasminogen content measuring device - uses determination method based on fibrin formation in plasma sample
EP0004256A1 (en) * 1978-02-07 1979-09-19 Kabi AB Easily split substrates for the quantification of proteases, a process for their production and a method for the quantification of proteases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2521206A1 (en) * 1975-05-13 1976-12-02 Eckart Dr Med Jacobi Plasminogen content measuring device - uses determination method based on fibrin formation in plasma sample
EP0004256A1 (en) * 1978-02-07 1979-09-19 Kabi AB Easily split substrates for the quantification of proteases, a process for their production and a method for the quantification of proteases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, Band 82, Nr. 3, 20. Januar 1975, Seite 169, Spalte 2, Abstract Nr. 12776p, Columbus, Ohio, USA; P.H. BELL et al.: "Sensitive fluorometric assay for plasminogen, plasmin and streptokinase"; & ANAL. BIOCHEM., Band 61, Nr. 1, 1974, Seiten 200-208 *
CHEMICAL ABSTRACTS, Band 92, Nr. 25, 23. Juni 1980, Seite 270, Spalte 1, Abstract Nr. 211314t, Columbus, Ohio, USA; M. KNOES et al.: "Methods for plasminogen determination in human plasma and for streptokinase standardization"; & PROG. CHEM. FIBRINOLYSIS THROMBOLYSIS, Band 4, 1979, Seiten 154-158 *
CHEMICAL ABSTRACTS, Band 99, Nr. 7, 15. August 1983, Seite 186, Spalte 2, Abstract Nr. 49324s, Columbus, Ohio, USA; S. Baba et al.: "An improved colorimetric assay of plasma plasminogen with chromogenic peptide substrate"; & RINSHO BYORI, Band 31, Nr. 3, 1983, Seiten 293-297 *

Also Published As

Publication number Publication date
ATE85364T1 (en) 1993-02-15
EP0264753B1 (en) 1993-02-03
CA1316088C (en) 1993-04-13
AU7980187A (en) 1988-04-21
JP2540563B2 (en) 1996-10-02
JPS63105698A (en) 1988-05-10
AU602535B2 (en) 1990-10-18
DE3635191A1 (en) 1988-04-21
ES2053496T3 (en) 1994-08-01
EP0264753A2 (en) 1988-04-27
DE3784000D1 (en) 1993-03-18

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