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EP0148333A1 - Einmalig verwendbare Pipettenspitze - Google Patents

Einmalig verwendbare Pipettenspitze Download PDF

Info

Publication number
EP0148333A1
EP0148333A1 EP84112251A EP84112251A EP0148333A1 EP 0148333 A1 EP0148333 A1 EP 0148333A1 EP 84112251 A EP84112251 A EP 84112251A EP 84112251 A EP84112251 A EP 84112251A EP 0148333 A1 EP0148333 A1 EP 0148333A1
Authority
EP
European Patent Office
Prior art keywords
tip
pipette
collar
pipette tip
disposable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP84112251A
Other languages
English (en)
French (fr)
Other versions
EP0148333B1 (de
Inventor
Fred Gardner Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to AT84112251T priority Critical patent/ATE45304T1/de
Publication of EP0148333A1 publication Critical patent/EP0148333A1/de
Application granted granted Critical
Publication of EP0148333B1 publication Critical patent/EP0148333B1/de
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means

Definitions

  • the present invention relates to disposable tips for a pipette to take up and discharge accurately minute liquid quantities at any given level.
  • a pipette tip that may be automatically picked up by and ejected from a reciprocable pipette.
  • Such tip is preferably formed of a nonwetting plastic with a conical take up tip portion terminating at its base end in a generally cylindrical portion to mate with the pipette barrel.
  • the cylindrical portion has (1) a stop, preferably a shoulder at its junction with the conical tip, that defines a seat for the end of the pipette barrel and establishes a known and repeatable distance from the end of the pipette barrel to the intake and discharge level of the tip and (2) an integral, internal circumferential seal between the stop and the open end of the cylinder to impose a low friction, air-tight seal around the pipette barrel.
  • Pipettes to handle precise small amounts of liquid are known in the chemical, biological and biochemical fields. They are particularly useful for dispensing and transferring microliter quantities of culture media, diluents, and other liquids from one location to another.
  • pipettes include a syringe with a reciprocable pump plunger operating in a barrel to aspirate liquid from or expel liquid into test tubes or wells in a microtiter tray through a replaceable tip.
  • Such pipettes have generally been used either single or as a "ganged" group for hand manipulation.
  • An automatic liquid transfer system that employs pipettes has recently been developed by applicant.
  • the system is particularly directed to pick up and dispose of a replaceable tip after each liquid transfer to avoid cross-contamination or liquid carryover from one liquid volume, or sample, to another. Because the entire system is adapted to sequence automatically through a number of steps, each of which requires (1) an air-tight connection between the tip and the pipette nose or barrel, with a predeterminable friction for easy installation and removal and (2) a given, fixed distance from the inlet end of the tip to the end of the pipette barrel.
  • the invention is a disposable pipette tip comprising an elongated conical tube, the small end of which defines a passageway for liquid and the large end of which receives the end of a pipette, characterized in that the tip includes a generally cylindrical collar for receiving the end of the pipette, the collar being axially aligned with and opening into the large end of the conical tube and having a stop for engaging the pipette to fix an accurately controlled distance from the end of the pipette to the small end of the pipette tip and a circumferential seal for forming a friction fit and seal with the sidewall of the pipette.
  • the stop of the collar provides a seat for the end of the pipette to provide a fixed and known length for the overall assembly. In this way. capillary distances, on the order of several hundredths of a centimeter, between the inlet end of the tip and the liquid surface can be machine controlled when the tip is withdrawn. Further the inlet end of the tip may be accurately positioned at any other desired or given level in a microtiter plate well, such as at an oil-water interface.
  • the circumferential seal between the lumen of the collar and the pipette barrel assures minimum friction with assured air sealing for tips within reasonable manufacturing tolerances.
  • the circumferential rib seal also assures easy removal of the tip for disposal after a single use, particularly where a number of pipettes are ganged together and a single stripper simultaneously ejects the tips from their respective pipettes.
  • disposable pipette tip 10 includes an elongated, hollow conical tube section 11 terminating at its intake and discharge end 12 at a right angle to the axis of conical section 11.
  • Base end 13 of conical tube 11 merges into a cylindrical mounting collar 14.
  • collar 14 provides a mounting and sealing arrangement with nose 15 of pipette barrel 16 carried by mounting block 18.
  • a pump plunger 22 is arranged to reciprocate in bore 24 of pipette barrel 16 to aspirate or discharge liquid through end 12 of tip 10.
  • lower end 19 of nose 15 seats on ledge or collar 17 formed by the transition between conical section 11 and cylindrical bore 20 of collar 14.
  • ledge or collar 17 formed by the transition between conical section 11 and cylindrical bore 20 of collar 14.
  • Such seating of end 19 on ledge 17 fixes a precise distance from block 18 to tip intake-discharge port 12.
  • radial projections other than a ledge or collar may be used to provide an axial stop for the end of the pipette barrel and that the projection need not be positioned at the transition between the conical and cylindrical section.
  • the stop may be defined by a rim at the mouth of the cylindrical section that is adapted to engage a radial collar or other radial member on the exterior of the pipette.
  • bore 20 of collar 14 includes an integral circumferential rib or ring 30.
  • rib 30 preferably has an axially deformed V-shape in cross section.
  • Such construction permits a low friction fit between the sidewalls of nose 15 and bore 20 of tip 10 both for insertion and removal while accomodating large manufacturing tolerance (several thousandths of a centimeter) in diameter and roundness of bore 20.
  • manufacturing tolerances are particularly vital in molding of tips 10 preferably formed of thermoplastic or thermosetting materials, such as polypropylene, polyvinylchloride, and fluorocarbon polymers.
  • the internal sidewall of the collar may have a slow taper that does not interfere with the action of the stop but causes the pipette to form a seal with the collar sidewall.
  • upper or open end 32 preferably has a bell-shaped mouth that terminates in a reinforced upper rim portion 34.
  • the bell mouth permits easy access for the end 19 of nose 15 so that if the tip is not in the center of hole 31, as in Figure 1. the cylindrical portions of nose 15 and bore 20 will readily align with each other.
  • Further rim portion 34 provides a pressing surface radially displaced from pipette nose 15 which is readily engageable by a stripper member 34 on an automatic stripper arm 36.
  • Replaceable tips in accordance with the present invention also include a plurality of three or more, preferably four, mounting splines 37, formed integral with the upper portion of conical section 11.
  • Splines 37 also provide thrust absorbing means so that the force of barrel 16 entering the collar and seating on ledge 17 does not place deforming stresses on intake-discharge end 12 of the tip.
  • splines 37 desirably extend axially along the exterior of the section 11 from transition ring 39, just below collar or ledge 17, and each terminates in the upper quarter or less of the conical section to form support legs to hold the tip, as shown.
  • splines 37 desirably extend axially along the exterior of the section 11 from transition ring 39, just below collar or ledge 17, and each terminates in the upper quarter or less of the conical section to form support legs to hold the tip, as shown.
  • transition ring 39 just below collar or ledge 17
  • spline ends 38 are at substantially right angles to the axis of the tip to hold the tip in such a vertical position when seated over hole 31 formed in a plate. tube or box support, such as 40, having a flat top.
  • hole 31 in box 40 is slightly larger than the greatest diameterof conical section 11 at the level of spline ends 38, but small enough to prevent wedging of splines 36 in hole 31.
  • splines 37 also provide minimum friction areas for absorbing thrust by ring 39 or cylindrical portion 14.
  • the length of splines 37 is such that their bottoms 38 are just above the axial center of gravity of tip 10 so that the tip does not wobble excessively in response to horizontal accelerations.
  • Such horizontal accelerations are important in high speed automatic liquid transfers and replacement of the tips on pipetter nose 15. This is because excess wobble of the tip when it is being loaded onto nose 15 would require a pause sufficient to assure alignment.
  • the cylindrical portions such as bore 20 and splines 37 will be formed with a draft angle of less than 2° relative to the axis.
  • nose portion 15 generally includes a small draft angle along its cylindrical portion to assure easy entrance and release with respect to bore 20 as well as for sealing action around rib 30. Because rib 30 is capable of radially deforming to conform to irregularities in the diameter or roundness of the side wall of nose 15, an air-tight seal is obtained without reliance solely upon deformation of the sidewall of cylindrical portion 14 or pressure between nose end 19 and collar 17.
  • Figure 6 illustrates alternate embodiments in the sealing and seating arrangements of the present invention.
  • seating collar 17 may be formed as a plurality of ledges 47 which are molded into tip 10 to form a fixed end seat for end 19. Desirably three or more, preferably four, ledges 47 are used in such an embodiment.
  • sealing between bore 20 and the cylindrical sidewall of nose 15 may be provided by an O-ring 48 seated in groove 49 formed in nose 15.
  • Figure 6 also illustrates that nose 15 may be formed with a slightly tapered end portion 50 which may be spherical in configuration. Alternatively the taper of portion 50 may be conical and the end slightly chamferred to permit easy alignment and passage of nose end 19 into tip 10, including an integrally formed sealing rib.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Cold Cathode And The Manufacture (AREA)
  • Materials For Medical Uses (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • External Artificial Organs (AREA)
EP84112251A 1983-10-13 1984-10-11 Einmalig verwendbare Pipettenspitze Expired EP0148333B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT84112251T ATE45304T1 (de) 1983-10-13 1984-10-11 Einmalig verwendbare pipettenspitze.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54167883A 1983-10-13 1983-10-13
US541678 1983-10-13

Publications (2)

Publication Number Publication Date
EP0148333A1 true EP0148333A1 (de) 1985-07-17
EP0148333B1 EP0148333B1 (de) 1989-08-09

Family

ID=24160607

Family Applications (1)

Application Number Title Priority Date Filing Date
EP84112251A Expired EP0148333B1 (de) 1983-10-13 1984-10-11 Einmalig verwendbare Pipettenspitze

Country Status (9)

Country Link
EP (1) EP0148333B1 (de)
JP (1) JPS60137443A (de)
AT (1) ATE45304T1 (de)
AU (1) AU565474B2 (de)
DE (2) DE3479298D1 (de)
DK (1) DK490984A (de)
FI (1) FI843976L (de)
NO (1) NO844093L (de)
NZ (1) NZ209865A (de)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293075A2 (de) * 1987-04-28 1988-11-30 Hewlett-Packard Company Pipette
EP0337726A2 (de) * 1988-04-12 1989-10-18 Flow Laboratories Limited Greifvorrichtung für Pipettenspitzen
EP0351574A2 (de) * 1988-07-21 1990-01-24 Eppendorf-Netheler-Hinz Gmbh Aufsteckbare Pipettenspitze in Form eines entsprechend einem Aufsteckkopfstück, insbesondere Konus einer Pipette, wenigstens abschnittsweise ausgeführten Gefässes
WO1991017833A2 (en) * 1990-05-21 1991-11-28 Pb Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
EP0611327A1 (de) * 1991-11-08 1994-08-24 Abbott Lab Pipettenspitze mit selbstjustierenden und selbstdichtenden vorrichtungen.
WO1995030483A1 (en) * 1994-05-06 1995-11-16 Sorenson Bioscience Filtered micropipette tip for high/low volume pipettors
EP0977039A2 (de) * 1998-07-27 2000-02-02 Hitachi, Ltd. Verfahren zur Handhabung von Körperflüssigkeitsproben und Analysevorrichtung. die diese verwendet
WO2000062933A1 (de) * 1999-04-16 2000-10-26 Hamilton Bonaduz Ag Pipettenspitze, pipettiervorrichtung und kombination aus pipettenspitze und pipettiervorrichtung
EP1126916A1 (de) * 1998-11-06 2001-08-29 Rainin Instrument LLC. Pipette mit verbesserter pipettenspitze und verbesserter montage-achse
WO2002057016A2 (en) * 2001-01-18 2002-07-25 Beckman Coulter, Inc. Pipette mandrel, pipette assembly and method for connecting a pipette mandrel to a pipette tip
US6596240B2 (en) 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
EP1329262A2 (de) * 2002-01-16 2003-07-23 Brand Gmbh + Co Kg Pipette und Verfahren zur Herstellung einer Pipette
EP1364711A2 (de) * 2002-05-22 2003-11-26 Eppendorf Ag Pipettenspitze
EP1884286A1 (de) * 1998-11-06 2008-02-06 Rainin Instrument LLC. Luftverschiebungspipette
WO2009058952A1 (en) * 2007-11-02 2009-05-07 Viaflo Corporation Locking pipette tip and mounting shaft
US7662343B2 (en) 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US8277757B2 (en) 2009-09-29 2012-10-02 Integra Biosciences Corp. Pipette tip mounting shaft
EP3885046A1 (de) 2006-05-29 2021-09-29 QIAGEN GmbH Vorrichtung zur halterung von pipettenspitzen und seine verwendung

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6780338B2 (ja) * 2016-07-22 2020-11-04 ぺんてる株式会社 ピペットチップとそのピペットチップに装着されるノズルとの連結構造

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2021566A1 (de) * 1968-10-25 1970-07-24 Bio Dynamics Inc
DE2114108A1 (de) * 1970-05-15 1971-11-25 Ollituote Oy Pipettenspitzenreihe,insbesondere zur maschinellen Fluessigkeitsdosierung
US3760639A (en) * 1972-10-20 1973-09-25 Bio Data Corp Pipette assembly
FR2305713A1 (fr) * 1975-03-25 1976-10-22 Faure Jean Distributeurs de volumes mesures de liquides
FR2313982A1 (fr) * 1975-06-12 1977-01-07 Eppendorf Geraetebau Netheler Embout rapportable de pipette

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2021566A1 (de) * 1968-10-25 1970-07-24 Bio Dynamics Inc
DE2114108A1 (de) * 1970-05-15 1971-11-25 Ollituote Oy Pipettenspitzenreihe,insbesondere zur maschinellen Fluessigkeitsdosierung
US3760639A (en) * 1972-10-20 1973-09-25 Bio Data Corp Pipette assembly
FR2305713A1 (fr) * 1975-03-25 1976-10-22 Faure Jean Distributeurs de volumes mesures de liquides
FR2313982A1 (fr) * 1975-06-12 1977-01-07 Eppendorf Geraetebau Netheler Embout rapportable de pipette

Cited By (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293075A3 (en) * 1987-04-28 1989-04-05 Hewlett-Packard Company Pipette assembly
EP0293075A2 (de) * 1987-04-28 1988-11-30 Hewlett-Packard Company Pipette
EP0337726A2 (de) * 1988-04-12 1989-10-18 Flow Laboratories Limited Greifvorrichtung für Pipettenspitzen
EP0337726A3 (de) * 1988-04-12 1990-09-26 Flow Laboratories Limited Greifvorrichtung für Pipettenspitzen
EP0351574A2 (de) * 1988-07-21 1990-01-24 Eppendorf-Netheler-Hinz Gmbh Aufsteckbare Pipettenspitze in Form eines entsprechend einem Aufsteckkopfstück, insbesondere Konus einer Pipette, wenigstens abschnittsweise ausgeführten Gefässes
EP0351574A3 (en) * 1988-07-21 1990-11-14 Eppendorf-Netheler-Hinz Gmbh Removable pipette tip shaped as a container adapted to the shape of a receiving piece, especially a tapered pipette end
WO1991017833A2 (en) * 1990-05-21 1991-11-28 Pb Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
WO1991017833A3 (en) * 1990-05-21 1991-12-26 Pb Diagnostic Systems Inc Fluid dispensing system having a pipette assembly with preset tip locator
US5200151A (en) * 1990-05-21 1993-04-06 P B Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
EP0611327B1 (de) * 1991-11-08 1997-05-28 Abbott Laboratories Pipettenspitze mit selbstjustierenden und selbstdichtenden vorrichtungen
EP0611327A1 (de) * 1991-11-08 1994-08-24 Abbott Lab Pipettenspitze mit selbstjustierenden und selbstdichtenden vorrichtungen.
WO1995030483A1 (en) * 1994-05-06 1995-11-16 Sorenson Bioscience Filtered micropipette tip for high/low volume pipettors
US5496523A (en) * 1994-05-06 1996-03-05 Sorenson Bioscience Filtered micropipette tip for high/low volume pipettors
EP0977039A2 (de) * 1998-07-27 2000-02-02 Hitachi, Ltd. Verfahren zur Handhabung von Körperflüssigkeitsproben und Analysevorrichtung. die diese verwendet
US7105351B2 (en) 1998-07-27 2006-09-12 Hitachi, Ltd. Handling method of body fluid sample and analysis apparatus using the same
EP0977039A3 (de) * 1998-07-27 2004-04-14 Hitachi, Ltd. Verfahren zur Handhabung von Körperflüssigkeitsproben und Analysevorrichtung. die diese verwendet
US6924152B2 (en) 1998-07-27 2005-08-02 Hitachi, Ltd. Handling method of body fluid sample and analysis apparatus using the same
EP1884286A1 (de) * 1998-11-06 2008-02-06 Rainin Instrument LLC. Luftverschiebungspipette
EP1126916A1 (de) * 1998-11-06 2001-08-29 Rainin Instrument LLC. Pipette mit verbesserter pipettenspitze und verbesserter montage-achse
EP1126916A4 (de) * 1998-11-06 2005-12-07 Rainin Instr Llc Pipette mit verbesserter pipettenspitze und verbesserter montage-achse
US7033543B1 (en) 1999-04-16 2006-04-25 Hamilton Bonaduz Ag Pipette tip, pipetting device and combination consisting of a pipette tip and pipetting device
US9415388B2 (en) 1999-04-16 2016-08-16 Hamilton Bonaduz Ag Pipette tip, pipette device, and combination of pipette tip and pipette device
DE19917375C2 (de) * 1999-04-16 2001-09-27 Hamilton Bonaduz Ag Bonaduz Pipettiereinheit
DE19917375A1 (de) * 1999-04-16 2000-11-02 Hamilton Bonaduz Ag Bonaduz Pipettenspitze, Pipettiervorrichtung und Kombination aus Pipettenspitze und Pipettiervorrichtung
WO2000062933A1 (de) * 1999-04-16 2000-10-26 Hamilton Bonaduz Ag Pipettenspitze, pipettiervorrichtung und kombination aus pipettenspitze und pipettiervorrichtung
EP1355738A1 (de) * 2001-01-12 2003-10-29 Porex Corporation Pipettenspitze zum leichten montieren und abwurf von einer pipette
US6596240B2 (en) 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
EP1355738A4 (de) * 2001-01-12 2006-04-12 Porex Corp Pipettenspitze zum leichten montieren und abwurf von einer pipette
WO2002057016A3 (en) * 2001-01-18 2003-04-10 Beckman Coulter Inc Pipette mandrel, pipette assembly and method for connecting a pipette mandrel to a pipette tip
WO2002057016A2 (en) * 2001-01-18 2002-07-25 Beckman Coulter, Inc. Pipette mandrel, pipette assembly and method for connecting a pipette mandrel to a pipette tip
US7344680B2 (en) 2002-01-16 2008-03-18 Brand Gmbh + Co Kg Pipette and process for producing a pipette
EP1329262A3 (de) * 2002-01-16 2004-04-07 Brand Gmbh + Co Kg Pipette und Verfahren zur Herstellung einer Pipette
EP1329262A2 (de) * 2002-01-16 2003-07-23 Brand Gmbh + Co Kg Pipette und Verfahren zur Herstellung einer Pipette
EP1364711A3 (de) * 2002-05-22 2005-01-12 Eppendorf Ag Pipettenspitze
EP1364711A2 (de) * 2002-05-22 2003-11-26 Eppendorf Ag Pipettenspitze
EP3885046A1 (de) 2006-05-29 2021-09-29 QIAGEN GmbH Vorrichtung zur halterung von pipettenspitzen und seine verwendung
US7662344B2 (en) 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US7662343B2 (en) 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US8501118B2 (en) 2006-10-24 2013-08-06 Integra Biosciences Corp. Disposable pipette tip
US8877513B2 (en) 2006-10-24 2014-11-04 Integra Biosciences Ag Method of using a disposable pipette tip
US9333500B2 (en) 2006-10-24 2016-05-10 Integra Biosciences Ag Locking pipette tip and mounting shaft in hand-held manual pipette
WO2009058952A1 (en) * 2007-11-02 2009-05-07 Viaflo Corporation Locking pipette tip and mounting shaft
US8277757B2 (en) 2009-09-29 2012-10-02 Integra Biosciences Corp. Pipette tip mounting shaft

Also Published As

Publication number Publication date
EP0148333B1 (de) 1989-08-09
DE3479298D1 (en) 1989-09-14
NO844093L (no) 1985-04-15
DK490984A (da) 1985-04-14
DK490984D0 (da) 1984-10-12
AU565474B2 (en) 1987-09-17
FI843976L (fi) 1985-04-14
JPS60137443A (ja) 1985-07-22
JPH0457377B2 (de) 1992-09-11
FI843976A0 (fi) 1984-10-10
ATE45304T1 (de) 1989-08-15
AU3418284A (en) 1985-04-18
DE148333T1 (de) 1986-01-16
NZ209865A (en) 1988-02-12

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