[go: up one dir, main page]

EA202193010A1 - Конвергентные жидкофазные синтезы олигонуклеотидов - Google Patents

Конвергентные жидкофазные синтезы олигонуклеотидов

Info

Publication number
EA202193010A1
EA202193010A1 EA202193010A EA202193010A EA202193010A1 EA 202193010 A1 EA202193010 A1 EA 202193010A1 EA 202193010 A EA202193010 A EA 202193010A EA 202193010 A EA202193010 A EA 202193010A EA 202193010 A1 EA202193010 A1 EA 202193010A1
Authority
EA
Eurasian Patent Office
Prior art keywords
oligonucleotides
phase synthesis
convergent
convergent liquid
liquid
Prior art date
Application number
EA202193010A
Other languages
English (en)
Inventor
Сянлинь Ши
Уильям Ф. Кисман
Фироз Антиа
Янник Филлон
Сюань ЧЖОУ
Умин Янь
Хун Цзянь
Хиен Нгуен
Роберт С. Гронке
Таисуке Ичимару
Такуя Хамагаки
Дайсуке Такахаши
Original Assignee
Байоджен Ма Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Байоджен Ма Инк. filed Critical Байоджен Ма Инк.
Publication of EA202193010A1 publication Critical patent/EA202193010A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

В изобретении описан конвергентный жидкофазный способ производства олигонуклеотидов путем связывания двух или более олигонуклеотидных фрагментов, каждый из которых имеет два или более нуклеотида. Изобретением также предусмотрены стадии реакции, вовлеченные в конвергентный жидкофазный способ.
EA202193010A 2019-05-08 2020-05-08 Конвергентные жидкофазные синтезы олигонуклеотидов EA202193010A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962845160P 2019-05-08 2019-05-08
PCT/US2020/032070 WO2020227618A2 (en) 2019-05-08 2020-05-08 Convergent liquid phase syntheses of oligonucleotides

Publications (1)

Publication Number Publication Date
EA202193010A1 true EA202193010A1 (ru) 2022-03-18

Family

ID=70919104

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202193010A EA202193010A1 (ru) 2019-05-08 2020-05-08 Конвергентные жидкофазные синтезы олигонуклеотидов

Country Status (13)

Country Link
US (1) US12378276B2 (ru)
EP (1) EP3966219A2 (ru)
JP (2) JP2022531876A (ru)
KR (1) KR20220007104A (ru)
CN (3) CN114206897B (ru)
AU (1) AU2020268415A1 (ru)
BR (1) BR112021022219A2 (ru)
CA (1) CA3139545A1 (ru)
EA (1) EA202193010A1 (ru)
IL (2) IL287798B2 (ru)
MA (1) MA55891A (ru)
MX (1) MX2021013587A (ru)
WO (1) WO2020227618A2 (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013202595B2 (en) 2012-03-30 2016-04-21 Biogen Ma Inc. Methods for modulating Tau expression for reducing seizure and modifying a neurodegenerative syndrome
TWI772856B (zh) 2013-07-19 2022-08-01 美商百健Ｍａ公司 用於調節τ蛋白表現之組合物
MX2023005467A (es) 2020-11-11 2023-07-27 Biogen Ma Inc Oligonucleotidos, reactivos y su preparacion.
US20250320493A1 (en) * 2021-07-23 2025-10-16 Biogen Ma Inc. Methods for reducing tau expression
WO2024083746A1 (en) 2022-10-17 2024-04-25 Bachem Holding Ag Method and composition for oligonucleotide synthesis
KR20250092173A (ko) * 2022-10-27 2025-06-23 스미또모 가가꾸 가부시끼가이샤 올리고뉴클레오티드의 제조 방법
WO2024185775A1 (ja) 2023-03-06 2024-09-12 スペラファーマ株式会社 長鎖アルケニルオキシ置換ベンゾイル誘導体、及び該誘導体を用いたオリゴヌクレオチド合成方法
GB202305283D0 (en) * 2023-04-11 2023-05-24 Exactmer Ltd Liquid-phase process for preparing oligonucleotides
JP7433684B1 (ja) * 2023-05-24 2024-02-20 塩野フィネス株式会社 疑似固相保護基、それを用いたヌクレオシド保護体又はオリゴヌクレオチド保護体、オリゴアミダイト前駆体の製造方法
EP4695265A1 (en) 2023-10-16 2026-02-18 Bachem Holding AG Method and composition for oligonucleotide synthesis
US20250206769A1 (en) * 2023-12-22 2025-06-26 Imec Vzw Synthesizing a Nucleic Acid or Nucleic Acid Analogue
WO2025235761A1 (en) 2024-05-09 2025-11-13 Biogen Ma Inc. Reagents and processes for preparing oligonucleotides

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05213986A (ja) * 1990-11-20 1993-08-24 Sankyo Co Ltd アンチセンス核酸誘導体
US5510476A (en) 1994-07-07 1996-04-23 Isis Pharmaceuticals, Inc. Carbocation scavenging during oligonucleotide synthesis
AU738032B2 (en) 1995-10-19 2001-09-06 Proligo Llc Method for solution phase synthesis of oligonucleotides
EP1386925A1 (en) * 2002-07-31 2004-02-04 Girindus AG Method for preparing oligonucleotides
PL2548560T3 (pl) 2005-06-23 2015-11-30 Biogen Ma Inc Kompozycje i sposoby modulowania splicingu SMN2
JP5645840B2 (ja) 2008-12-02 2014-12-24 株式会社Ｗａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｊａｐａｎ リン原子修飾核酸の合成方法
JP5548852B2 (ja) 2009-05-29 2014-07-16 国立大学法人東京農工大学 疎水性基結合ヌクレオシド、疎水性基結合ヌクレオシド溶液、及び疎水性基結合オリゴヌクレオチド合成方法
KR101965868B1 (ko) 2009-06-17 2019-04-05 바이오젠 엠에이 인코포레이티드 대상에게서 ｓｍｎ２ 스플라이싱을 조정하기 위한 조성물 및 방법
US20120184724A1 (en) * 2009-09-22 2012-07-19 Agilent Technologies, Inc. Protected monomers and methods of deprotection for rna synthesis
JP5705512B2 (ja) * 2010-11-26 2015-04-22 農工大ティー・エル・オー株式会社 高分散性液相支持体を用いたオリゴヌクレオチド合成法
CN103476784B (zh) * 2011-05-17 2017-05-24 味之素株式会社 制备寡核苷酸的方法
US8846885B2 (en) 2012-02-17 2014-09-30 Ajinomoto Co., Inc. Oligonucleotide with protected base
WO2013122236A1 (ja) * 2012-02-17 2013-08-22 味の素株式会社 塩基部保護オリゴヌクレオチド
DK2857412T3 (en) 2012-05-30 2017-04-03 Hokkaido System Science Co Ltd Method for Oligonucleotide Synthesis Using High-Resolution Liquid Phase Carrier
CN104918949B (zh) * 2012-11-14 2017-10-10 武田药品工业株式会社 核酸的液相合成方法
SI3126499T1 (sl) 2014-04-01 2020-09-30 Biogen Ma Inc. Sestave za moduliranje izražanja SOD-1
CN107207555B (zh) 2015-01-21 2021-10-22 味之素株式会社 沉淀促进剂以及使用其的沉淀方法
CN116063365A (zh) * 2015-12-16 2023-05-05 味之素株式会社 寡核苷酸的制备方法以及核苷、核苷酸或寡核苷酸
WO2017111137A1 (ja) 2015-12-22 2017-06-29 味の素株式会社 オリゴヌクレオチドの製造方法
BR112018009312A8 (pt) 2015-12-28 2019-02-26 Chugai Pharmaceutical Co Ltd método para promover eficiência de purificação de polipeptídeo contendo região de fc
JP7306263B2 (ja) 2017-05-02 2023-07-11 日産化学株式会社 オリゴヌクレオチドの製造方法

Also Published As

Publication number Publication date
AU2020268415A1 (en) 2021-12-23
IL319933A (en) 2025-05-01
CN119143828A (zh) 2024-12-17
IL287798B1 (en) 2025-05-01
US12378276B2 (en) 2025-08-05
WO2020227618A2 (en) 2020-11-12
US20220348602A1 (en) 2022-11-03
MA55891A (fr) 2022-03-16
CN119462804A (zh) 2025-02-18
CA3139545A1 (en) 2020-11-12
AU2020268415A8 (en) 2022-01-20
BR112021022219A2 (pt) 2022-03-22
MX2021013587A (es) 2022-02-11
IL287798A (en) 2022-01-01
JP2025138846A (ja) 2025-09-25
WO2020227618A8 (en) 2020-12-24
JP2022531876A (ja) 2022-07-12
CN114206897B (zh) 2024-09-20
EP3966219A2 (en) 2022-03-16
CN114206897A (zh) 2022-03-18
KR20220007104A (ko) 2022-01-18
WO2020227618A3 (en) 2021-01-14
IL287798B2 (en) 2025-09-01

Similar Documents

Publication Publication Date Title
EA202193010A1 (ru) Конвергентные жидкофазные синтезы олигонуклеотидов
EP3398955A4 (en) OLIGONUCLEOTIDE MANUFACTURING METHOD AND NUCLEOSIDE, NUCLEOTIDE OR OLIGONUCLEOTIDE
FR3023840B1 (fr) Procede de production de paraxylene a haute purete a partir d'une coupe xylenes, utilisant deux unites de separation en lit mobile simule fonctionnant en serie et deux unites d'isomerisation.
SG11201809961TA (en) Variants of a dna polymerase of the polx family
SA518400053B1 (ar) عملية مُحسنة لإنتاج حمض أكريليك
WO2016160844A3 (en) Methods and compositions for combinatorial barcoding
MX2009008958A (es) Metodo para separar halocarburos.
EP3330243A4 (en) PROCESS FOR PREPARING 1-CHLORO-2,3,3-TRIFLUORPROPENE
MY190981A (en) Biomass-derived chemical intermediates
EP3995581A3 (en) Oligonucleotide compounds for treatment of preeclampsia and other angiogenic disorders
BR112017009754A2 (pt) processo para produção de hexanotriol a partir de 5-hidroximetfilfurfural
EP3778619A4 (en) SEGMENT FOR OLIGONUCLEOTIDE SYNTHESIS, MANUFACTURING METHODS THEREFORE, AND OLIGONUCLEOTIDE SYNTHESIS METHOD USING THE SAME
MX389335B (es) Procedimiento de produccion de e-1-cloro-3,3,3-trifluoropropeno a partir de 1,1,3,3-tetracloropropeno.
IL277346A (en) A method for creating a hairpin single-helical RNA molecule
IL292191A (en) Photochemical process for producing (4r,4s)-4-(4-cyano-2-methoxyphenyl)-5-ethoxy-2,8-dimethyl-1,4-dihydro-1,6-naphthyridin-3-carboxamide
EP3929205A4 (en) PROCESS FOR PREPARING MODIFIED OLIGONUCLEOTIDE WITH COMPLEMENTARY SEQUENCE
MX374209B (es) Metodo para producir 1,1,3,3-tetracloropropeno.
WO2019122879A3 (en) Methods for performing biological reactions
MX2017013749A (es) Composiciones y metodos para construir genotecas de adnc especificas de hebra.
DE602006012971D1 (de) Vermittelter chemie
EP3388147A4 (en) DEHYDRATION CATALYST FOR AN ALKYLAROMATIC COMPOUND, PROCESS FOR THE PREPARATION THEREOF AND DEHYDRATION METHOD THEREWITH
EP3995475A4 (en) PROCESS FOR PURIFICATION OF 1,3-BUTADIENE
EP3466540A4 (en) CATALYST COMPOSITION FOR HYDROFORMULATION REACTION AND METHOD FOR THE PRODUCTION OF ALDEHYDE THEREWITH
MX2015012249A (es) Metodo para producir cis-1-cloro-3,3,3-trifluoropropeno.
EP3464227A4 (en) PROCESSES FOR PRODUCING SOLVENTS DERIVED FROM 1-CHLORO -3, 3-TRIFLUORO-PROPENE (1233ZD)