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EA200600579A1 - Способ получения плазмидной днк фармацевтического качества - Google Patents

Способ получения плазмидной днк фармацевтического качества

Info

Publication number
EA200600579A1
EA200600579A1 EA200600579A EA200600579A EA200600579A1 EA 200600579 A1 EA200600579 A1 EA 200600579A1 EA 200600579 A EA200600579 A EA 200600579A EA 200600579 A EA200600579 A EA 200600579A EA 200600579 A1 EA200600579 A1 EA 200600579A1
Authority
EA
Eurasian Patent Office
Prior art keywords
plasmid dna
order
purification steps
pharmaceutical quality
pdna
Prior art date
Application number
EA200600579A
Other languages
English (en)
Other versions
EA009447B1 (ru
Inventor
Франсис БЛАНШ
Мишель Кудер
Никола Маестрали
Дейвид Геллак
Тьери Гиймен
Original Assignee
Сентельон
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Сентельон filed Critical Сентельон
Publication of EA200600579A1 publication Critical patent/EA200600579A1/ru
Publication of EA009447B1 publication Critical patent/EA009447B1/ru

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Manufacturing & Machinery (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Согласно данному изобретению предложен способ непрерывного щелочного лизиса бактериальной суспензии для получения пДНК. Согласно ему дополнительно предложены возможные дополнительные стадии, включающие фильтрацию лизата, анионообменную хроматографию, аффинную хроматографию с образованием тройной спирали и гидрофобную хроматографию. Эти возможные стадии очистки можно комбинировать с непрерывным лизисом для того, чтобы получить продукт высокоочищенной плазмидной пДНК, по существу лишенной гДНК, РНК, белка, эндотоксина и других примесей.
EA200600579A 2003-09-17 2004-09-17 Способ получения плазмидной днк фармацевтического качества EA009447B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50346403P 2003-09-17 2003-09-17
US56300804P 2004-04-19 2004-04-19
PCT/EP2004/011437 WO2005026331A2 (en) 2003-09-17 2004-09-17 Method of preparation of pharmaceutically grade plasmid dna

Publications (2)

Publication Number Publication Date
EA200600579A1 true EA200600579A1 (ru) 2006-10-27
EA009447B1 EA009447B1 (ru) 2007-12-28

Family

ID=42827706

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600579A EA009447B1 (ru) 2003-09-17 2004-09-17 Способ получения плазмидной днк фармацевтического качества

Country Status (17)

Country Link
US (1) US20070111221A1 (ru)
EP (1) EP1664277B1 (ru)
JP (1) JP2007505613A (ru)
KR (1) KR101094032B1 (ru)
CN (1) CN1882682B (ru)
AT (1) ATE532853T1 (ru)
AU (2) AU2004272748C1 (ru)
BR (1) BRPI0413907A (ru)
CA (1) CA2536931A1 (ru)
EA (1) EA009447B1 (ru)
ES (1) ES2376173T3 (ru)
IL (1) IL174147A0 (ru)
MX (1) MXPA06003004A (ru)
NO (1) NO20061659L (ru)
NZ (1) NZ545449A (ru)
PT (1) PT1664277E (ru)
WO (1) WO2005026331A2 (ru)

Cited By (1)

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RU2489168C1 (ru) * 2012-02-28 2013-08-10 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция, продуцирующая антиоксидантный, антимикробный, антитоксический белок - лактоферрин человека, способ ее получения и способ терапии

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CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
EP3452574A1 (en) 2016-05-06 2019-03-13 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Plug flow tubular bioreactor, system containing the same and method for production of virus
US20210207075A1 (en) * 2018-05-31 2021-07-08 Vgxi Inc. Lysis coil apparatus and uses thereof for isolation and purification of polynucleotides
IL296722A (en) 2020-03-31 2022-11-01 Richter Helm Biologics Gmbh & Co Kg Methods for the production of plasmid DNA
CN111394427B (zh) * 2020-04-22 2023-04-11 宁波市博坤生物科技有限公司 一种含泥土检材中微量dna的提取方法
EP3906982B1 (de) 2020-05-08 2022-03-16 AXAGARIUS GmbH & Co. KG Verfahren zur plasmidreinigung unter gleichzeitiger abreicherung von endotoxinen
JP2021074018A (ja) * 2021-02-12 2021-05-20 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ プラグフローチューブ型バイオリアクター、それを含むシステム、およびウイルスの調製のための方法
CN115404232A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的方法
CN113332869B (zh) * 2021-06-23 2022-12-23 苏州沃美生物有限公司 混合装置和方法以及重组大肠杆菌质粒提取方法
WO2024086213A2 (en) * 2022-10-19 2024-04-25 Abec, Inc. Cell lysis and plasmid isolation system
CN117187069B (zh) * 2023-09-18 2024-04-05 苏州依科赛生物科技股份有限公司 一种碱裂解工艺的大肠杆菌hcp检测方法

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* Cited by examiner, † Cited by third party
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RU2489168C1 (ru) * 2012-02-28 2013-08-10 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция, продуцирующая антиоксидантный, антимикробный, антитоксический белок - лактоферрин человека, способ ее получения и способ терапии
WO2013129960A1 (ru) * 2012-02-28 2013-09-06 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция, продуцирующая лактоферрин человека, способ её получения и способ терапии

Also Published As

Publication number Publication date
CN1882682B (zh) 2010-06-16
EA009447B1 (ru) 2007-12-28
HK1096994A1 (en) 2007-06-15
AU2004272748C1 (en) 2010-07-08
BRPI0413907A (pt) 2006-10-24
EP1664277B1 (en) 2011-11-09
AU2004272748B2 (en) 2009-12-03
NZ545449A (en) 2009-07-31
MXPA06003004A (es) 2006-06-23
ES2376173T3 (es) 2012-03-09
PT1664277E (pt) 2012-01-17
CA2536931A1 (en) 2005-03-24
AU2010200801A1 (en) 2010-03-25
KR20060086941A (ko) 2006-08-01
CN1882682A (zh) 2006-12-20
US20070111221A1 (en) 2007-05-17
KR101094032B1 (ko) 2011-12-19
JP2007505613A (ja) 2007-03-15
AU2004272748A1 (en) 2005-03-24
ATE532853T1 (de) 2011-11-15
WO2005026331A3 (en) 2005-11-17
IL174147A0 (en) 2006-08-01
WO2005026331A2 (en) 2005-03-24
NO20061659L (no) 2006-06-13
EP1664277A2 (en) 2006-06-07

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): BY RU