DK430486A - Fremgangsmaade til udvinding af protein - Google Patents
Fremgangsmaade til udvinding af protein Download PDFInfo
- Publication number
- DK430486A DK430486A DK430486A DK430486A DK430486A DK 430486 A DK430486 A DK 430486A DK 430486 A DK430486 A DK 430486A DK 430486 A DK430486 A DK 430486A DK 430486 A DK430486 A DK 430486A
- Authority
- DK
- Denmark
- Prior art keywords
- dialysis
- denaturant
- resolubilized
- protein
- extraction
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000000605 extraction Methods 0.000 title 1
- 238000000502 dialysis Methods 0.000 abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 210000003000 inclusion body Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/773,671 US4656255A (en) | 1985-09-09 | 1985-09-09 | Protein recovery |
Publications (2)
Publication Number | Publication Date |
---|---|
DK430486D0 DK430486D0 (da) | 1986-09-09 |
DK430486A true DK430486A (da) | 1987-03-10 |
Family
ID=25098959
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK430486A DK430486A (da) | 1985-09-09 | 1986-09-09 | Fremgangsmaade til udvinding af protein |
Country Status (9)
Country | Link |
---|---|
US (1) | US4656255A (da) |
EP (1) | EP0215625B1 (da) |
JP (1) | JP2646081B2 (da) |
AT (1) | ATE113960T1 (da) |
CA (1) | CA1267497A (da) |
DE (1) | DE3650135T2 (da) |
DK (1) | DK430486A (da) |
IE (1) | IE67259B1 (da) |
IL (1) | IL79972A (da) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5340926A (en) * | 1983-03-25 | 1994-08-23 | Celltech, Limited | Process for the recovery of recombinantly produced protein from insoluble aggregate |
BG49718A3 (en) * | 1983-07-15 | 1992-01-15 | Bio- Technology General Corp | Method for preparing of polypeptid with superoxiddismutasne activitty |
US5332805A (en) * | 1984-02-03 | 1994-07-26 | Celltech Limited | Process for the recovery of recombinantly produced chymosin from insoluble aggregate |
US5792450A (en) * | 1985-02-05 | 1998-08-11 | Chiron Corporation | Purified human CSF-1 |
GB8508340D0 (en) * | 1985-03-29 | 1985-05-09 | Creighton T E | Production of protein |
US4748234A (en) * | 1985-06-26 | 1988-05-31 | Cetus Corporation | Process for recovering refractile bodies containing heterologous proteins from microbial hosts |
AU597924B2 (en) * | 1985-12-11 | 1990-06-14 | Natinco Nv | Solubilization of protein aggregates |
DE3611817A1 (de) * | 1986-04-08 | 1987-10-15 | Boehringer Mannheim Gmbh | Verfahren zur renaturierung von proteinen |
US4705848A (en) * | 1986-06-02 | 1987-11-10 | International Minerals & Chemical Corp. | Isolation of bioactive, monomeric growth hormone |
CA1339757C (en) * | 1987-04-16 | 1998-03-17 | Robert F. Halenbeck | Production of purified biologically active, bacterially produced recombinant human csf-1 |
US4929700A (en) * | 1987-04-16 | 1990-05-29 | Cetus Corporation | Production of purified, biologically active, bacterially produced recombinant human CSF-1 |
US5162507A (en) * | 1987-05-11 | 1992-11-10 | Cetus Corporation | Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms |
WO1988008849A1 (en) * | 1987-05-11 | 1988-11-17 | Cetus Corporation | Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms |
US4931543A (en) * | 1987-05-11 | 1990-06-05 | Cetus Corporation | Process for recovering microbially produced interleukin-2 |
US4975529A (en) * | 1989-08-18 | 1990-12-04 | Monsanto Company | Method of folding somatotropins |
US5530100A (en) * | 1990-05-07 | 1996-06-25 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Methods for purification of recombinantly produced proteins |
US5849694A (en) * | 1990-07-16 | 1998-12-15 | Synenki; Richard M. | Stable and bioactive modified porcine somatotropin and pharmaceutical compositions thereof |
DK0686162T3 (da) * | 1993-02-04 | 2003-09-22 | Borean Pharma As | Forbedret fremgangsmåde til refoldning af proteiner |
US6153406A (en) * | 1993-07-23 | 2000-11-28 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B |
US5437986A (en) * | 1994-06-22 | 1995-08-01 | Schering Corporation | Extraction of heterologous insoluble proteins from bacteria |
US5912327A (en) * | 1996-09-30 | 1999-06-15 | Human Genome Sciences, Inc. | Method of purifying chemokines from inclusion bodies |
US6632425B1 (en) | 1997-03-20 | 2003-10-14 | Human Genome Sciences, Inc. | Chemokine compositions |
CN114957378B (zh) * | 2022-04-15 | 2024-02-13 | 深圳重链生物科技有限公司 | 疏水性包涵体抗原蛋白的复性方法 |
CN116178481A (zh) * | 2022-11-28 | 2023-05-30 | 青岛润达生物科技有限公司 | 一种包涵体蛋白的复性方法及用途 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4511502A (en) * | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
GR79124B (da) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
US4518526A (en) * | 1982-12-22 | 1985-05-21 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4511503A (en) * | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
-
1985
- 1985-09-09 US US06/773,671 patent/US4656255A/en not_active Expired - Fee Related
-
1986
- 1986-09-08 EP EP86306917A patent/EP0215625B1/en not_active Expired - Lifetime
- 1986-09-08 IL IL79972A patent/IL79972A/xx not_active IP Right Cessation
- 1986-09-08 CA CA000517714A patent/CA1267497A/en not_active Expired
- 1986-09-08 DE DE3650135T patent/DE3650135T2/de not_active Expired - Fee Related
- 1986-09-08 AT AT86306917T patent/ATE113960T1/de not_active IP Right Cessation
- 1986-09-08 IE IE238486A patent/IE67259B1/en not_active IP Right Cessation
- 1986-09-08 JP JP61209746A patent/JP2646081B2/ja not_active Expired - Lifetime
- 1986-09-09 DK DK430486A patent/DK430486A/da not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CA1267497C (en) | 1990-04-03 |
IL79972A (en) | 1991-05-12 |
EP0215625A2 (en) | 1987-03-25 |
JPS6277332A (ja) | 1987-04-09 |
DE3650135T2 (de) | 1995-06-14 |
IE862384L (en) | 1987-03-09 |
IL79972A0 (en) | 1986-12-31 |
EP0215625A3 (en) | 1989-04-12 |
US4656255A (en) | 1987-04-07 |
IE67259B1 (en) | 1996-03-06 |
ATE113960T1 (de) | 1994-11-15 |
CA1267497A (en) | 1990-04-03 |
DE3650135D1 (de) | 1994-12-15 |
JP2646081B2 (ja) | 1997-08-25 |
EP0215625B1 (en) | 1994-11-09 |
DK430486D0 (da) | 1986-09-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK430486D0 (da) | Fremgangsmaade til udvinding af protein | |
DK426386A (da) | Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer | |
DK0526544T3 (da) | Terapeutiske anvendelser af actinbindende forbindelser | |
DK394786A (da) | Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer | |
DE3587549D1 (de) | Ergänzungstestverfahren von proteine bindenden enzymen. | |
ATE69108T1 (de) | Elektrophoretisches gel hoher aufloesung sowie methode zur abscheidung von serumproteinen. | |
UA26375A1 (uk) | Спосіб очищеhhя іhсуліhу | |
GR3018371T3 (en) | Method for identifying or determining proteins and applications therefor. | |
ATE119543T1 (de) | Produktion von proteinen in aktiver form. | |
HK1004336A1 (en) | Isolation and purification of lung surfactant protein | |
FI881122A (fi) | Menetelmä tyvikalvoproteiinien eristämiseksi ihmis- ja eläinkudoksista | |
DK513687D0 (da) | Monoklonale antistoffer over for ifn-omega, fremgangsmaade til deres fremstilling og deres anvendelse til rensning samt paavisning af ifn-omega | |
FI885025A (fi) | Koagulationshaemmande protein pp4-x, dess framstaellning och anvaendning. | |
DK142380A (da) | Fremgangsmaade til fremstilling af pentadecapeptider | |
GB1475262A (en) | Human urinary trypsin inhibitor and a method of preparing it | |
ATE134218T1 (de) | Methode zur reinigung rekombinanter t-pa von den ihren entsprechenden wirtszellproteinen | |
DK447784D0 (da) | Immuninterferon | |
DK205188D0 (da) | Proteinprotease-inhibitorer af streptomyces | |
FI934079A (fi) | Menetelmä fuusioproteiinin valmistamiseksi | |
ATE93546T1 (de) | Ergaenzungstestverfahren von proteine bindenden enzymen. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AHS | Application shelved for other reasons than non-payment |