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DK3559267T3 - Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller - Google Patents

Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller Download PDF

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Publication number
DK3559267T3
DK3559267T3 DK17817859.6T DK17817859T DK3559267T3 DK 3559267 T3 DK3559267 T3 DK 3559267T3 DK 17817859 T DK17817859 T DK 17817859T DK 3559267 T3 DK3559267 T3 DK 3559267T3
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nucleic acids
single cells
bar coding
molecular bar
molecular
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DK17817859.6T
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Lucas Brandon Edelman
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Cs Genetics Ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
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    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
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    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DK17817859.6T 2016-12-23 2017-12-19 Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller DK3559267T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1622222.6A GB201622222D0 (en) 2016-12-23 2016-12-23 Reagents and methods for molecular barcoding of nucleic acids of single cells
PCT/GB2017/053816 WO2018115852A1 (en) 2016-12-23 2017-12-19 Reagents and methods for molecular barcoding of nucleic acids of single cells

Publications (1)

Publication Number Publication Date
DK3559267T3 true DK3559267T3 (da) 2021-07-12

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DK21168174.7T DK3957744T5 (da) 2016-12-23 2017-12-19 Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer
DK17817859.6T DK3559267T3 (da) 2016-12-23 2017-12-19 Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller

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US (3) US12084714B2 (da)
EP (3) EP3559267B1 (da)
DK (2) DK3957744T5 (da)
ES (2) ES2880394T3 (da)
GB (1) GB201622222D0 (da)
HR (1) HRP20211078T1 (da)
SI (1) SI3559267T1 (da)
WO (1) WO2018115852A1 (da)

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Publication number Publication date
EP4265723A2 (en) 2023-10-25
US20190316182A1 (en) 2019-10-17
US20240327896A1 (en) 2024-10-03
US12084714B2 (en) 2024-09-10
DK3957744T5 (da) 2024-07-15
SI3559267T1 (sl) 2021-08-31
ES2955997T3 (es) 2023-12-11
GB201622222D0 (en) 2017-02-08
ES2880394T3 (es) 2021-11-24
US11845924B1 (en) 2023-12-19
EP3559267A1 (en) 2019-10-30
EP3957744B1 (en) 2023-06-28
DK3957744T3 (da) 2023-09-18
EP3957744A1 (en) 2022-02-23
EP3559267B1 (en) 2021-04-14
EP4265723A3 (en) 2024-01-10
WO2018115852A1 (en) 2018-06-28
HRP20211078T1 (hr) 2021-10-01

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