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DK2678432T3 - Rekombinante mikroorganismer og anvendelser deraf - Google Patents

Rekombinante mikroorganismer og anvendelser deraf Download PDF

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Publication number
DK2678432T3
DK2678432T3 DK12749472.2T DK12749472T DK2678432T3 DK 2678432 T3 DK2678432 T3 DK 2678432T3 DK 12749472 T DK12749472 T DK 12749472T DK 2678432 T3 DK2678432 T3 DK 2678432T3
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Sean Dennis Simpson
Michael Koepke
Fungmin Liew
Wendy Yiting Chen
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Lanzatech New Zealand Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C12P7/28—Acetone-containing products
    • C12P7/30—Acetone-containing products produced from substrate containing inorganic compounds other than water
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01074—Indolepyruvate decarboxylase (4.1.1.74)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/10—Process efficiency
    • Y02P20/133—Renewable energy sources, e.g. sunlight

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Microbiology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (11)

1. Fremgangsmåde til fremstilling af isopropanol ved mikrobiel fermentering, der omfatter fermentering af et substrat, der omfatter CO, ved anvendelse af en rekombinant carboxydotrof eddikesyredannende mikroorganisme, der er i stand til at frembringe isopropanol ved fermentation af CO, hvor mikroorganismen udtrykker enzymerne: acetyl-coenzym A-acetyltransferase (thiolase; ThlA; E.C. 2.3.1.9); acetoacetyl-CoA:acetat-coenzym A-transferase A (CoA-transferase; CtfA; EC 2.8.3.9); og acetoacetyl-CoA:acetat-coenzym A-transferase B (CoA-transferase; CtfB; EC 2.8.3.9); og hvor mikroorganismen endvidere udtrykker enzymet: a. acetoacetat-decarboxylase (Adc; EC 4.1.1.4) eller b. alpha-ketoisovalerat-decarboxylase (decarboxylase; KivD; EC4.1.1.74); hvor enzymerne ikke er naturligt til stede i en parental mikroorganisme, hvoraf den rekombinante mikroorganisme er afledt.
2. Fremgangsmåde ifølge krav la, hvor mikroorganismen endvidere udtrykker enzymet alkoholdehydrogenase (Adh; EC 1.1.1.2).
3. Fremgangsmåde ifølge krav 2, hvor Adh udtrykkes eksogent.
4. Fremgangsmåde ifølge krav la, hvor mikroorganismen endvidere udtrykker enzymet alkoholdehydrogenase (Adh2; EC 1.1.1.1).
5. Fremgangsmåde ifølge krav 4, hvor Adh2 udtrykkes eksogent.
6. Fremgangsmåde ifølge krav la, hvor mikroorganismen endvidere udtrykker enzymerne alpha-ketoisovalerat-decarboxylase (decarboxylase; KivD; EC4.1.1.74) og alkoholdehydrogenase (Adh2; EC 1.1.1.1).
7. Fremgangsmåde ifølge krav 6, hvor Adh2 udtrykkes eksogent.
8. Fremgangsmåde ifølge krav 1, hvor den parentale mikroorganisme vælges fra gruppen af carboxydotrofe eddikesyredannende bakterier, der omfatter: Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdalei, Clostridium carboxidivorans, Clostridium drakei, Clostridium scatologenes, Butyribacterium limosum, Butyribacterium methylotrophicum, Acetobacterium woodii, Alkalibaculum bacchii, Blautia producta, Eubacterium limosum, Moorella thermoacetica, Moorella thermautotrophica, Oxobacter pfetmigii og Thermoanaerobacter kiuvi.
9. Fremgangsmåde ifølge krav 6, hvor parentale mikroorganisme er Clostridium autoethanogenum DSM23693 eller Clostridium ljungdahlii DSM13528 (eller ATCC55383).
10. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor enzymet: a. ThlA er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 18; og b. CtfA er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 19; og c. CtfB er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 20 og/eller d. Adc er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 21; og/eller e. KivD er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 71; og/eller f. Adh er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 2, 3 eller 4; og/eller g. Adh2 er kodet for af et nukleotid, der omfatter sekvensen ifølge SEQ ID NO: 75.
11. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor: a. det gasformige substrat, der omfatter CO, fremstilles ved en industriel proces valgt fra gruppen bestående af fremstilling af jernholdige metalprodukter, fremstilling af ikke-jemholdige produkter, olieraffineringsprocesser, forgasning af kul, forgasning af biomasse, elkraftproduktion, carbon black-produktion og koksfremstilling; eller b. det gasformige substrat omfatter 20 % til 70 % CO efter volumen.
DK12749472.2T 2011-02-25 2012-02-24 Rekombinante mikroorganismer og anvendelser deraf DK2678432T3 (da)

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US201161446832P 2011-02-25 2011-02-25
PCT/NZ2012/000022 WO2012115527A2 (en) 2011-02-25 2012-02-24 Recombinant microorganisms and uses therefor

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EP (2) EP3401405A1 (da)
JP (1) JP6199747B2 (da)
KR (2) KR102015672B1 (da)
CN (2) CN103502435B (da)
AU (1) AU2012221176B2 (da)
BR (1) BR112013021524A2 (da)
CA (2) CA2825267C (da)
DK (1) DK2678432T3 (da)
ES (1) ES2702621T3 (da)
MY (2) MY193665A (da)
PL (1) PL2678432T4 (da)
TW (1) TWI541348B (da)
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Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013152236A1 (en) 2012-04-05 2013-10-10 Lanzatech New Zealand Limited Enzyme-altered metabolite activity
US20130316364A1 (en) 2012-05-23 2013-11-28 Lanzatech New Zealand Limited Selection Method and Recombinant Microorganisms and uses Therefor
CA2874832C (en) 2012-05-30 2016-02-02 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
WO2013185123A1 (en) 2012-06-08 2013-12-12 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
EP3004362A4 (en) * 2013-06-05 2017-01-11 Lanzatech New Zealand Limited Recombinant microorganisms exhibiting increased flux through a fermentation pathway
EA033071B1 (ru) * 2013-09-22 2019-08-30 Ланцатек Нью Зилэнд Лимитед Способ увеличения выхода продуктов из ацетолактата
US20150152445A1 (en) * 2013-12-03 2015-06-04 Lanzatech New Zealand Limited Microorganisms and methods for the production of ketones
WO2015116734A1 (en) 2014-01-28 2015-08-06 Lanzatech New Zealand Limited Method of producing a recombinant microorganism
EP4180806A1 (en) 2014-10-22 2023-05-17 LanzaTech NZ, Inc. Bioreactor and gas testing unit and method
CA2965368C (en) 2014-10-22 2018-08-14 Lanzatech New Zealand Limited Multi-stage bioreactor processes
PL3230459T3 (pl) 2014-12-08 2021-03-08 Lanzatech New Zealand Limited Rekombinowane mikroorganizmy wykazujące zwiększony strumień przez szlak fermentacji
TWI739734B (zh) 2015-02-23 2021-09-21 紐西蘭商藍瑟科技紐西蘭有限公司 用於將甲烷轉化為產物之重組產醋酸細菌
WO2017066498A1 (en) 2015-10-13 2017-04-20 Lanzatech New Zealand Limited Genetically engineered bacterium comprising energy-generating fermentation pathway
EA201891335A1 (ru) 2015-12-03 2018-11-30 Ланцатек Нью Зилэнд Лимитед Добавление аргинина с целью повышения эффективности ацетогенов, ферментирующих газ
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
ES2955708T3 (es) 2016-02-01 2023-12-05 Lanzatech Nz Inc Proceso integrado de fermentación y electrólisis
EP3420089B1 (en) 2016-02-26 2021-12-29 LanzaTech NZ, Inc. Crispr/cas systems for c-1 fixing bacteria
WO2017200884A1 (en) 2016-05-14 2017-11-23 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
EP3679149B1 (en) 2017-09-08 2022-06-29 Lanzatech, Inc. Processes for metabolite production using hydrogen rich c1-containing substrates
WO2019157507A1 (en) 2018-02-12 2019-08-15 Lanzatech, Inc. A process for improving carbon conversion efficiency
ES2960999T3 (es) 2018-04-20 2024-03-07 Lanzatech Inc Proceso de fermentación para producir etanol
CN112955558A (zh) 2018-11-19 2021-06-11 朗泽科技有限公司 发酵和气化的集成
MY196166A (en) 2019-02-08 2023-03-17 Lanzatech Inc Process For Recovering Close Boiling Products
EP3942058A1 (en) 2019-03-20 2022-01-26 Global Bioenergies Improved means and methods for producing isobutene from acetyl-coa
WO2021006995A1 (en) 2019-07-11 2021-01-14 Lanzatech, Inc. Methods for optimizing gas utilization
CN111057686B (zh) * 2019-12-23 2021-05-04 浙江大学 一种醇脱氢酶突变体及应用
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
AU2021237368A1 (en) 2020-03-18 2022-10-06 Lanzatech, Inc. Fermentative production of 2-phenylethanol from gaseous substrates
KR20220164810A (ko) 2020-06-06 2022-12-13 란자테크, 인크. 아세토락테이트 탈카복실화효소 유전자위에 녹인이 있는 미생물
WO2022066997A1 (en) * 2020-09-25 2022-03-31 Lanzatech, Inc. Recombinant microorganisms and uses therefor
EP4208554A4 (en) 2021-02-08 2024-03-20 Lanzatech, Inc. RECOMBINANT MICROORGANISMS AND THEIR USES
CN117098850A (zh) 2021-04-09 2023-11-21 朗泽科技有限公司 用于提供原料的方法和设备
CN114561416B (zh) * 2022-04-27 2022-07-15 深圳中科翎碳生物科技有限公司 利用电能固定co2及合成异丙醇的工程菌及构建方法
US12129503B2 (en) 2022-08-10 2024-10-29 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
WO2024231219A1 (en) 2023-05-11 2024-11-14 Evonik Operations Gmbh Biotechnological production of acetone

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
ES2267794T3 (es) 2000-07-25 2007-03-16 Emmaus Foundation, Inc. Metodo para incrementar la produccion de etanol a partir de fermentacion microbioana.
US7851188B2 (en) * 2005-10-26 2010-12-14 Butamax(Tm) Advanced Biofuels Llc Fermentive production of four carbon alcohols
NZ546496A (en) * 2006-04-07 2008-09-26 Lanzatech New Zealand Ltd Gas treatment process
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
EP2198039A4 (en) * 2007-10-12 2012-05-09 Univ California MODIFIED MICROORGANISMS FOR THE MANUFACTURE OF ISOPROPANOL
KR101375029B1 (ko) * 2007-11-13 2014-03-14 란자테크 뉴질랜드 리미티드 신규 세균 및 이의 이용 방법
BRPI0909334B1 (pt) * 2008-03-12 2019-10-29 Lanzatech New Zealand Ltd método para a conversão de ácido em álcool correspondente
KR20110021797A (ko) * 2008-04-25 2011-03-04 자이단호우진 치큐칸쿄 산교기쥬츠 켄큐키코 이소프로판올을 제조할 수 있는 유전자 변형된 코리네형 세균
EP2373781A4 (en) * 2008-12-16 2012-10-10 Genomatica Inc MICRO-ORGANISMS AND METHODS OF CONVERTING SYNGAS AND OTHER CARBON RESOURCES IN USEFUL PRODUCTS
DE102009002583A1 (de) * 2009-04-23 2010-10-28 Evonik Degussa Gmbh Zellen und Verfahren zur Herstellung von Aceton
NZ624392A (en) * 2009-08-21 2015-12-24 Lallemand Hungary Liquidity Man Llc Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii

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EP2678432A2 (en) 2014-01-01
EP2678432B1 (en) 2018-08-01
WO2012115527A3 (en) 2013-01-10
KR20190097292A (ko) 2019-08-20
PL2678432T3 (pl) 2019-05-31
ES2702621T3 (es) 2019-03-04
CA2995872A1 (en) 2012-08-30
CA2825267A1 (en) 2012-08-30
WO2012115527A2 (en) 2012-08-30
CN108486169A (zh) 2018-09-04
CA2825267C (en) 2018-04-03
NZ614459A (en) 2015-09-25
TW201309795A (zh) 2013-03-01
CN103502435B (zh) 2018-05-18
CN103502435A (zh) 2014-01-08
PL2678432T4 (pl) 2019-05-31
CN108486169B (zh) 2022-08-12
BR112013021524A2 (pt) 2017-06-13
KR102121886B1 (ko) 2020-06-15
AU2012221176A1 (en) 2013-05-09
TWI541348B (zh) 2016-07-11
KR20140012106A (ko) 2014-01-29
EP3401405A1 (en) 2018-11-14
MY193665A (en) 2022-10-24
MY168208A (en) 2018-10-15
JP6199747B2 (ja) 2017-09-20
EP2678432A4 (en) 2016-06-22
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KR102015672B1 (ko) 2019-08-29
AU2012221176B2 (en) 2015-03-19

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