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DK2638149T3 - Immortalisering af epitelceller og fremgangsmåder til anvendelse - Google Patents

Immortalisering af epitelceller og fremgangsmåder til anvendelse Download PDF

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Publication number
DK2638149T3
DK2638149T3 DK11839723.1T DK11839723T DK2638149T3 DK 2638149 T3 DK2638149 T3 DK 2638149T3 DK 11839723 T DK11839723 T DK 11839723T DK 2638149 T3 DK2638149 T3 DK 2638149T3
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Prior art keywords
immortalization
methods
epithel
cells
epithel cells
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DK11839723.1T
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English (en)
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Richard Schlegel
Xuefeng Liu
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Univ Georgetown
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0683—Cells of the male genital tract, e.g. prostate, epididymis; Non-germinal cells from testis, e.g. Leydig cells, Sertoli cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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DK11839723.1T 2010-11-12 2011-11-11 Immortalisering af epitelceller og fremgangsmåder til anvendelse DK2638149T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41329110P 2010-11-12 2010-11-12
US201161474901P 2011-04-13 2011-04-13
PCT/US2011/060378 WO2012065067A2 (en) 2010-11-12 2011-11-11 Immortalization of epithelial cells and methods of use

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DK2638149T3 true DK2638149T3 (da) 2019-08-12

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US (5) US9279106B2 (da)
EP (2) EP2638149B1 (da)
JP (2) JP6076258B2 (da)
CA (1) CA2817712C (da)
DK (1) DK2638149T3 (da)
WO (1) WO2012065067A2 (da)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2638149B1 (en) * 2010-11-12 2019-05-15 Georgetown University Immortalization of epithelial cells and methods of use
WO2014082096A1 (en) * 2012-11-26 2014-05-30 The Trustees Of Columbia University In The City Of New York Method for culture of human and mouse prostate organoids and uses thereof
AU2014209218B2 (en) * 2013-01-25 2018-06-07 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
WO2014172340A1 (en) * 2013-04-15 2014-10-23 Georgetown University Immortalization of circulating tumor cells and methods of use
WO2015192035A1 (en) * 2014-06-13 2015-12-17 Georgetown University Compositions and methods for immortalization of epithelial cells
MX2017000181A (es) * 2014-06-27 2017-05-01 Univ California Celulas madre limbales de mamifero cultivadas, metodos para generarlas y sus usos.
KR102276424B1 (ko) 2014-10-06 2021-07-12 삼성전자주식회사 로-키나제 저해제를 포함하는 세포의 노화를 감소시키기 위한 조성물 및 그의 용도
JP6964068B2 (ja) 2015-04-03 2021-11-10 プロパジェニクス インコーポレイテッド 上皮細胞のエキソビボ増殖
US10100285B2 (en) 2015-04-03 2018-10-16 Propagenix Inc. Ex vivo proliferation of epithelial cells
JP6869231B2 (ja) 2015-09-11 2021-05-12 プロパジェニクス インコーポレイテッド ｅｘ ｖｉｖｏでの上皮細胞の増殖
EP3426772A4 (en) 2016-03-09 2019-08-28 Beijing Percans Oncology Co. Ltd. TUMOR CELLSUSPENSION CULTURES AND RELATED METHODS
US20190151371A1 (en) * 2016-06-09 2019-05-23 Georgetown University Process for continuous cell culture of islet cells
CN107267446A (zh) * 2017-07-27 2017-10-20 广州齐志生物工程设备有限公司 一种应用于条件性重编程技术的饲养细胞的处理方法
CN110093310B (zh) * 2018-01-29 2021-07-02 中国科学院动物研究所 一种将成纤维细胞转化为永生化细胞的方法及其应用
US12163156B2 (en) 2018-01-29 2024-12-10 Institute Of Zoology, Chinese Academy Of Sciences Cell induction method
CN110093309B (zh) * 2018-01-29 2021-07-02 中国科学院动物研究所 一种诱导成纤维细胞转分化为脂肪细胞的方法
WO2019199881A1 (en) * 2018-04-09 2019-10-17 Cedars-Sinai Medical Center Methods for in vitro expansion of adult tissue stem cells
WO2019196606A1 (en) * 2018-04-13 2019-10-17 Primordial Biotech. Co. Method for obtaining an animal model from conditionally reprogrammed cells and use of the animal model for screening anti-tumor drugs
CN112126627B (zh) * 2020-09-30 2022-08-19 扬州大学 一种犬角膜上皮细胞永生化细胞系的构建方法和应用
CN114836383B (zh) * 2021-02-01 2024-06-25 合肥中科普瑞昇生物医药科技有限公司 肠癌原代细胞的培养基及培养方法
JP7635661B2 (ja) 2021-07-09 2025-02-26 横河電機株式会社 判定装置、判定方法、および判定プログラム
US20250027050A1 (en) * 2021-11-12 2025-01-23 Takahiro Ochiya Long-term culture of colorectal epithelial cells
CN117025505A (zh) * 2022-05-10 2023-11-10 上海赛立维生物科技有限公司 胃粘膜上皮前体样细胞及其制备方法和应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665710A (en) 1990-04-30 1997-09-09 Georgetown University Method of making liposomal oligodeoxynucleotide compositions
JP3220180B2 (ja) 1991-05-23 2001-10-22 三菱化学株式会社 薬剤含有タンパク質結合リポソーム
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
US5756122A (en) 1995-06-07 1998-05-26 Georgetown University Liposomally encapsulated nucleic acids having high entrapment efficiencies, method of manufacturer and use thereof for transfection of targeted cells
WO1997004787A1 (en) 1995-08-01 1997-02-13 Novartis Ag Liposomal oligonucleotide compositions
US7217722B2 (en) 2000-02-01 2007-05-15 Kirin Beer Kabushiki Kaisha Nitrogen-containing compounds having kinase inhibitory activity and drugs containing the same
EP1403255A4 (en) 2001-06-12 2005-04-06 Sumitomo Pharma RHO KINASE INHIBITORS
ES2305435T3 (es) 2002-01-10 2008-11-01 Bayer Healthcare Ag Inhibidores de la rho-quinasa.
US6924290B2 (en) 2002-01-23 2005-08-02 Bayer Pharmaceuticals Corporation Rho-kinase inhibitors
DE60318177T2 (de) 2002-01-23 2008-10-09 Bayer Pharmaceuticals Corp., West Haven Rho-kinase inhibitoren
TW200306819A (en) 2002-01-25 2003-12-01 Vertex Pharma Indazole compounds useful as protein kinase inhibitors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
US7645878B2 (en) 2002-03-22 2010-01-12 Bayer Healthcare Llc Process for preparing quinazoline Rho-kinase inhibitors and intermediates thereof
AU2003220935A1 (en) 2002-04-03 2003-10-13 Sumitomo Pharmaceuticals Company, Limited. Benzamide derivatives
ES2273047T3 (es) 2002-10-28 2007-05-01 Bayer Healthcare Ag Fenilaminopirimidinas sustituidas con heteroariloxi como inhibidores de rho-cinasa.
US7560467B2 (en) 2003-06-19 2009-07-14 Smithkline Beecham Corporation Indazolo-tetrahydropyrimidine-carboxamide derivative kinase inhibitors
JP2007516196A (ja) 2003-07-02 2007-06-21 ガラパゴス エヌブイ Ｒｈｏキナーゼ阻害剤としてのピラジン及びピラリジン誘導体
US20100016329A1 (en) * 2008-07-21 2010-01-21 Kaohsiung Medical University Xanthine-based cyclic gmp-enhancing rho-kinase inhibitor inhibits physiological activities of lung epithelial cell line
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CA2907326A1 (en) 2008-11-04 2010-05-14 Chad Green Stem cell aggregate suspension compositions and methods for differentiation thereof
WO2010059775A1 (en) 2008-11-20 2010-05-27 Centocor Ortho Biotech Inc. Pluripotent stem cell culture on micro-carriers
US8637310B2 (en) * 2008-12-05 2014-01-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
DK2421957T3 (da) * 2009-04-22 2021-01-25 Viacyte Inc Cellesammensætninger afledt af dedifferentierede omprogrammerede celler
WO2011137485A1 (en) * 2010-05-05 2011-11-10 Sydney Ivf Limited Media and methods for cell culture
EP2638149B1 (en) * 2010-11-12 2019-05-15 Georgetown University Immortalization of epithelial cells and methods of use

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EP2638149A4 (en) 2014-09-17
US11001808B2 (en) 2021-05-11
CA2817712C (en) 2020-03-24
JP6076258B2 (ja) 2017-02-08
EP2638149A2 (en) 2013-09-18
US9279106B2 (en) 2016-03-08
JP2014502151A (ja) 2014-01-30
US20150099267A1 (en) 2015-04-09
US10041048B2 (en) 2018-08-07
US9657272B2 (en) 2017-05-23
US20130309681A1 (en) 2013-11-21
US9951315B2 (en) 2018-04-24
WO2012065067A2 (en) 2012-05-18
US20160289644A1 (en) 2016-10-06
CA2817712A1 (en) 2012-05-18
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