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DK2610345T3 - Rekombinante influenzaviruslignende partikler (vlps), som er fremstillet i transgene planter, der udtrykker hæmagglutinin - Google Patents

Rekombinante influenzaviruslignende partikler (vlps), som er fremstillet i transgene planter, der udtrykker hæmagglutinin Download PDF

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DK2610345T3
DK2610345T3 DK12183854.4T DK12183854T DK2610345T3 DK 2610345 T3 DK2610345 T3 DK 2610345T3 DK 12183854 T DK12183854 T DK 12183854T DK 2610345 T3 DK2610345 T3 DK 2610345T3
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Denmark
Prior art keywords
seq
plant
influenza
protein
virus
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DK12183854.4T
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Aoust Marc-Andre D
Manon Couture
Frédéric Ors
Sonia Trépanier
Pierre-Olivier Lavoie
Michéle Dargis
LOUIS-PHILIPPE VéZINA
Nathalie Landry
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Medicago Inc
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Priority claimed from CA002615372A external-priority patent/CA2615372A1/en
Application filed by Medicago Inc filed Critical Medicago Inc
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Publication of DK2610345T3 publication Critical patent/DK2610345T3/da

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Claims (15)

1. Nukleinsyre, som omfatter en nukleotidsekvens, der koder for et influenzahæmagglutinin (influenza-HA), hvor nukleotidsekvensen omfatter et regulatorelement, som er operativt i en plante, hvilket regulatorelement omfatter en vignabønnemosaikvirus (Cowpea Mosaic Virus - CPMV)-regulatorregion.
2. Nukleinsyre ifølge krav 1, hvor HA'et omfatter et nativt eller et ikke-nativt signalpeptid.
3. Nukleinsyre ifølge krav 2, hvor det ikke-native signalpeptid er et proteindisulfidisomerase-signalpeptid.
4. Nukleinsyre ifølge et hvilket som helst af kravene 1-3, hvor HA'et er et influenza-HA af type A, type B eller en undertype af type A valgt fra gruppen bestående af H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15 og H16, fortrinsvis H1, H2, H3, H5, H6, H7og H9.
5. Nukleinsyre ifølge krav 1, hvor nukleotidsekvensen, der koder for influenzahæmagglutininet (influenza-HA'et) udviser 70-100% sekvensidentitet med nukleotidsekvensen ifølge SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 eller SEQ ID NO: 47.
6. Fremgangsmåde til fremstilling af en influenzaviruslignende partikel (VLP) i en plante, hvilken fremgangsmåde omfatter: a) indføring af nukleinsyren ifølge et hvilket som helst af kravene 1-5 i en plante eller en plantedel eller a) tilvejebringelse af en plante eller en plantedel, som omfatter nukleinsyren ifølge et hvilket som helst af kravene 1-5, og b) inkubering af planten eller plantedelen under betingelser, som muliggør ekspressionen af nukleinsyren, idet VLP'en derved fremstilles, og eventuelt c) høst af planten og oprensning af VLP'erne.
7. Fremgangsmåde ifølge krav 6, hvor nukleinsyren i trin a): a) udtrykkes kortvarigt i planten eller b) udtrykkes stabilt i planten.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 6-7, hvor, i trin a), en anden nukleinsyre, som omfatter en nukleotidsekvens, der koder for ét eller mere end ét chaperoneprotein, indføres i planten, idet chaperoneproteinet eller chaperoneproteinerne fortrinsvis er valgt fra gruppen bestående af Flsp40 og Flsp70.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 6-8, hvor VLP'en har en størrelse i intervallet 80-300 nm.
10. Plante, som omfatter nukleinsyren ifølge et hvilket som helst af kravene 1-5.
11. Plante ifølge krav 10, der endvidere omfatter en nukleinsyre, som omfatter en nukleotidsekvens, der koder for ét eller mere end ét chaperoneprotein, som er operativt bundet til en regulatorregion, der er aktiv i en plante, idet chaperoneproteinet eller chaperoneproteinerne fortrinsvis er valgt fra gruppen bestående af Hsp40 og Hsp70.
12. Viruslignende partikel (VLP), som er fremstillet ved fremgangsmåden ifølge et hvilket som helst af kravene 6-8 og omfatter et influenzavirushæmagglutinin (HA)-protein, hvilken VLP har en størrelse i intervallet 80-300 nm, og a) ét eller mere end ét lipid, som stammer fra en plante; eller b) plantespecifikke N-glycaner eller modificerede N-glycaner.
13. Sammensætning, som omfatter en dosis af VLP'en ifølge krav 12, som er effektiv til fremkaldelse af immunitet mod et influenzavirus hos et individ, og en farmaceutisk acceptabel bærer.
14. Viruslignende partikel ifølge krav 12 eller sammensætning ifølge krav 13 til anvendelse til fremkaldelse af immunitet mod en influenzavirusinfektion hos et individ.
15. Viruslignende partikel eller sammensætning ifølge krav 14, hvor den viruslignende partikel er fremstillet til administration til individet oralt, intradermalt, intranasalt, intramuskulært, intraperitonealt, intravenøst eller subkutant.
DK12183854.4T 2007-11-27 2009-01-12 Rekombinante influenzaviruslignende partikler (vlps), som er fremstillet i transgene planter, der udtrykker hæmagglutinin DK2610345T3 (da)

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