DK174772B1 - Fremgangsmåde til fremstilling af nucleotider og hertil anvendelige forbindelser - Google Patents

Fremgangsmåde til fremstilling af nucleotider og hertil anvendelige forbindelser Download PDF

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Publication number: DK174772B1
Authority: DK; Denmark
Prior art keywords: formula; group; denotes; different; see diagramm
Prior art date: 1985-03-06

Application number

DK198601009A

Other languages

Danish (da)

English (en)

Other versions

DK100986A (da

DK100986D0 (da

Inventor

Eugen Uhlmann

Joachim Engels

Original Assignee

Hoechst Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-03-06

Filing date

1986-03-05

Publication date

2003-10-27

1986-03-05 Application filed by Hoechst Ag filed Critical Hoechst Ag

1986-03-05 Publication of DK100986D0 publication Critical patent/DK100986D0/da

1986-09-07 Publication of DK100986A publication Critical patent/DK100986A/da

2003-10-27 Application granted granted Critical

2003-10-27 Publication of DK174772B1 publication Critical patent/DK174772B1/da

Links

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125000003729 nucleotide group Chemical group 0.000 title claims description 9
238000000034 method Methods 0.000 title abstract description 15
238000002360 preparation method Methods 0.000 title abstract description 8
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 21
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 20
125000000217 alkyl group Chemical group 0.000 claims abstract description 18
125000004432 carbon atom Chemical group C* 0.000 claims abstract description 18
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims abstract description 16
229910052757 nitrogen Inorganic materials 0.000 claims abstract description 14
125000001424 substituent group Chemical group 0.000 claims abstract description 13
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LINDOXZENKYESA-UHFFFAOYSA-N TMG Natural products CNC(N)=NC LINDOXZENKYESA-UHFFFAOYSA-N 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
230000010933 acylation Effects 0.000 description 1
238000005917 acylation reaction Methods 0.000 description 1
239000003513 alkali Substances 0.000 description 1
229910052783 alkali metal Inorganic materials 0.000 description 1
150000001340 alkali metals Chemical class 0.000 description 1
125000002947 alkylene group Chemical group 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
125000004429 atom Chemical group 0.000 description 1
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
238000009835 boiling Methods 0.000 description 1
125000001246 bromo group Chemical group Br* 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
239000000969 carrier Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
239000005289 controlled pore glass Substances 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000006642 detritylation reaction Methods 0.000 description 1
238000001035 drying Methods 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
239000003480 eluent Substances 0.000 description 1
238000001914 filtration Methods 0.000 description 1
239000000499 gel Substances 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
229930195733 hydrocarbon Natural products 0.000 description 1
150000002430 hydrocarbons Chemical class 0.000 description 1
AFQIYTIJXGTIEY-UHFFFAOYSA-N hydrogen carbonate;triethylazanium Chemical compound OC(O)=O.CCN(CC)CC AFQIYTIJXGTIEY-UHFFFAOYSA-N 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
239000011261 inert gas Substances 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
239000011630 iodine Substances 0.000 description 1
125000002346 iodo group Chemical group I* 0.000 description 1
JJWLVOIRVHMVIS-UHFFFAOYSA-N isopropylamine Chemical compound CC(C)N JJWLVOIRVHMVIS-UHFFFAOYSA-N 0.000 description 1
150000002634 lipophilic molecules Chemical class 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
CAAULPUQFIIOTL-UHFFFAOYSA-N methyl dihydrogen phosphate Chemical group COP(O)(O)=O CAAULPUQFIIOTL-UHFFFAOYSA-N 0.000 description 1
XUJBUAFHPKSYHS-UHFFFAOYSA-N methyl dihydrogen phosphate;dihydrochloride Chemical compound Cl.Cl.COP(O)(O)=O XUJBUAFHPKSYHS-UHFFFAOYSA-N 0.000 description 1
239000000178 monomer Substances 0.000 description 1
125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 description 1
239000005445 natural material Substances 0.000 description 1
150000002825 nitriles Chemical class 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
230000000269 nucleophilic effect Effects 0.000 description 1
239000007800 oxidant agent Substances 0.000 description 1
150000002978 peroxides Chemical class 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
125000006245 phosphate protecting group Chemical group 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
239000011591 potassium Substances 0.000 description 1
239000000843 powder Substances 0.000 description 1
125000006308 propyl amino group Chemical group 0.000 description 1
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
239000002342 ribonucleoside Substances 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
JRPHGDYSKGJTKZ-UHFFFAOYSA-N selenophosphoric acid Chemical group OP(O)([SeH])=O JRPHGDYSKGJTKZ-UHFFFAOYSA-N 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
QDRKDTQENPPHOJ-UHFFFAOYSA-N sodium ethoxide Chemical compound [Na+].CC[O-] QDRKDTQENPPHOJ-UHFFFAOYSA-N 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
125000001302 tertiary amino group Chemical group 0.000 description 1
CIHOLLKRGTVIJN-UHFFFAOYSA-N tert‐butyl hydroperoxide Chemical compound CC(C)(C)OO CIHOLLKRGTVIJN-UHFFFAOYSA-N 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
125000002480 thymidyl group Chemical group 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
150000005691 triesters Chemical class 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
238000005292 vacuum distillation Methods 0.000 description 1
239000011701 zinc Substances 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/141—Esters of phosphorous acids
- C07F9/146—Esters of phosphorous acids containing P-halide groups
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
- C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
- C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
- C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
- C07F9/2429—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of arylalkanols
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/26—Amides of acids of phosphorus containing P-halide groups

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Steroid Compounds (AREA)
Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
Saccharide Compounds (AREA)
Luminescent Compositions (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

DK198601009A 1985-03-06 1986-03-05 Fremgangsmåde til fremstilling af nucleotider og hertil anvendelige forbindelser DK174772B1 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE19853507881 DE3507881A1 (de)	1985-03-06	1985-03-06	Verfahren und mittel zur phosphorylierung
DE3507881		1985-03-06

Publications (3)

Publication Number	Publication Date
DK100986D0 DK100986D0 (da)	1986-03-05
DK100986A DK100986A (da)	1986-09-07
DK174772B1 true DK174772B1 (da)	2003-10-27

Family

ID=6264335

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198601009A DK174772B1 (da)	1985-03-06	1986-03-05	Fremgangsmåde til fremstilling af nucleotider og hertil anvendelige forbindelser

Country Status (17)

Country	Link
US (1)	US5039796A (fi)
EP (1)	EP0195277B1 (fi)
JP (2)	JPH0643433B2 (fi)
KR (1)	KR940005338B1 (fi)
AT (1)	ATE44151T1 (fi)
AU (1)	AU596091B2 (fi)
CA (1)	CA1340950C (fi)
DE (2)	DE3507881A1 (fi)
DK (1)	DK174772B1 (fi)
ES (1)	ES8801293A1 (fi)
FI (1)	FI81806C (fi)
GR (1)	GR860600B (fi)
HU (1)	HU202247B (fi)
IE (1)	IE58874B1 (fi)
IL (1)	IL78033A (fi)
PT (1)	PT82139B (fi)
ZA (1)	ZA861629B (fi)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
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US5332845A (en) *	1985-03-28	1994-07-26	Chiron Corporation	Phosphorylating reagents
US5071974A (en) *	1986-10-31	1991-12-10	Amoco Corporation	Compositions and methods for the synthesis of oligonucleotides having 5'-phosphorylated termini
US5585481A (en) *	1987-09-21	1996-12-17	Gen-Probe Incorporated	Linking reagents for nucleotide probes
US6031091A (en) *	1987-09-21	2000-02-29	Gen-Probe Incorporated	Non-nucleotide linking reagents for nucleotide probes
SE8802574D0 (sv) *	1988-07-08	1988-07-08	Wallac Oy	Oligonucleotide hybridization probes and means for the synthesis of the most preferred probes
US5380835A (en) *	1991-06-07	1995-01-10	Abbott Laboratories	Silyl phosphorylating reagents and methods of using them
US5872244A (en) *	1994-09-02	1999-02-16	Andrew C. Hiatt	3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5763594A (en) *	1994-09-02	1998-06-09	Andrew C. Hiatt	3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6214987B1 (en)	1994-09-02	2001-04-10	Andrew C. Hiatt	Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US6232465B1 (en)	1994-09-02	2001-05-15	Andrew C. Hiatt	Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5808045A (en) *	1994-09-02	1998-09-15	Andrew C. Hiatt	Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5990300A (en) *	1994-09-02	1999-11-23	Andrew C. Hiatt	Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6184347B1 (en)	1998-11-19	2001-02-06	Agilent Technologies Inc.	Minimization of blooming in high-density arrays by using reactive wash reagents
CA2370478A1 (en)	1999-03-24	2000-09-28	Serge L. Beaucage	N-acylphosphoramidites and their use in oligonucleotide synthesis
WO2003048179A2 (en) *	2001-12-03	2003-06-12	The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services	Thermolabile hydroxyl protecting groups and methods of use
GB0304371D0 (en)	2003-02-26	2003-04-02	Solexa Ltd	DNA Sequence analysis
US9809824B2 (en)	2004-12-13	2017-11-07	The United States Of America, Represented By The Secretary, Department Of Health And Human Services	CpG oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
DE102009012640A1 (de)	2009-03-09	2010-09-30	Freie Universität Berlin	Mit einer Phosphoamidatgruppe modifizierte Verbindung und deren Verwendung
US10128538B2 (en)	2014-07-09	2018-11-13	Nec Corporation	Non-aqueous electrolytic solution and lithium ion secondary battery

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE1075584B (de) *		1960-02-18	Hooker Chemical Corporation, Niagara Falls, N. Y. (V. St. A.)	Verfahren zur Herstellung von tertiären aliphatischen Estern der phosphorigen Säure
DE1252648B (de) *	1962-01-29	1967-10-26	Hooker Chemical Corporation, Niagara Falls NY (V St A)	Verfahren zur Herstellung von Tnmethyl phosphit
US4321365A (en) *	1977-10-19	1982-03-23	Research Corporation	Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4415732A (en) *	1981-03-27	1983-11-15	University Patents, Inc.	Phosphoramidite compounds and processes
NL8302353A (nl)	1983-07-01	1985-02-01	Stichting Katholieke Univ	Fosforyleringsmiddel; werkwijze voor het bereiden daarvan; werkwijze voor het fosforyleren van een organische hydroxyl- of amineverbinding.
EP0133993B1 (en)	1983-08-19	1991-06-19	General Electric Company	Modified polyester composition
DE3678988D1 (de) *	1985-03-28	1991-06-06	Chiron Corp	Reinigung von synthetischen oligomeren.

1985
- 1985-03-06 DE DE19853507881 patent/DE3507881A1/de not_active Withdrawn
1986
- 1986-02-22 DE DE8686102311T patent/DE3664047D1/de not_active Expired
- 1986-02-22 EP EP86102311A patent/EP0195277B1/de not_active Expired
- 1986-02-22 AT AT86102311T patent/ATE44151T1/de not_active IP Right Cessation
- 1986-03-04 GR GR860600A patent/GR860600B/el unknown
- 1986-03-04 ES ES552614A patent/ES8801293A1/es not_active Expired
- 1986-03-04 IL IL78033A patent/IL78033A/xx unknown
- 1986-03-04 FI FI860905A patent/FI81806C/fi not_active IP Right Cessation
- 1986-03-04 HU HU86889A patent/HU202247B/hu unknown
- 1986-03-05 AU AU54311/86A patent/AU596091B2/en not_active Expired
- 1986-03-05 CA CA000503382A patent/CA1340950C/en not_active Expired - Fee Related
- 1986-03-05 ZA ZA861629A patent/ZA861629B/xx unknown
- 1986-03-05 KR KR860001541A patent/KR940005338B1/ko not_active Expired - Lifetime
- 1986-03-05 IE IE57986A patent/IE58874B1/en not_active IP Right Cessation
- 1986-03-05 JP JP61046486A patent/JPH0643433B2/ja not_active Expired - Lifetime
- 1986-03-05 PT PT82139A patent/PT82139B/pt unknown
- 1986-03-05 DK DK198601009A patent/DK174772B1/da not_active IP Right Cessation
1989
- 1989-08-21 US US07/396,803 patent/US5039796A/en not_active Expired - Lifetime
1993
- 1993-09-29 JP JP5242250A patent/JP2549495B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
ES8801293A1 (es)	1987-12-16
FI81806B (fi)	1990-08-31
HUT40675A (en)	1987-01-28
FI860905L (fi)	1986-09-07
ES552614A0 (es)	1987-12-16
CA1340950C (en)	2000-04-11
HU202247B (en)	1991-02-28
EP0195277A1 (de)	1986-09-24
DE3507881A1 (de)	1986-09-11
ATE44151T1 (de)	1989-07-15
IL78033A (en)	1990-12-23
JP2549495B2 (ja)	1996-10-30
JPH0643433B2 (ja)	1994-06-08
IE58874B1 (en)	1993-12-01
ZA861629B (en)	1986-10-29
AU596091B2 (en)	1990-04-26
DK100986A (da)	1986-09-07
KR860007273A (ko)	1986-10-10
JPS61207397A (ja)	1986-09-13
JPH06228172A (ja)	1994-08-16
FI860905A0 (fi)	1986-03-04
PT82139B (pt)	1988-07-29
IE860579L (en)	1986-09-06
GR860600B (en)	1986-07-07
US5039796A (en)	1991-08-13
EP0195277B1 (de)	1989-06-21
AU5431186A (en)	1986-10-16
DK100986D0 (da)	1986-03-05
KR940005338B1 (en)	1993-06-17
DE3664047D1 (en)	1989-07-27
PT82139A (de)	1986-04-01
FI81806C (fi)	1990-12-10
IL78033A0 (en)	1986-07-31

Publication	Publication Date	Title
DK174772B1 (da)	2003-10-27	Fremgangsmåde til fremstilling af nucleotider og hertil anvendelige forbindelser
JP2552048B2 (ja)	1996-11-06	ヌクレオチド鎖合成用試薬
JP5143872B2 (ja)	2013-02-13	ホスホネートヌクレオシド誘導体
US5847106A (en)	1998-12-08	Monomeric and dimeric nucleosides with silyl-containing diamino phosphorous linkages
US5367066A (en)	1994-11-22	Oligonucleotides with selectably cleavable and/or abasic sites
US5252760A (en)	1993-10-12	Method of using colored phosphorylating reagents
NZ225944A (en)	1991-04-26	Biotinylated polynucleotides and analogues thereof, processes for their preparation and intermediates therefor
JPS6072899A (ja)	1985-04-24	ヌクレオシドアルキル‐、アルアルキル‐およびアリール‐ホスホナイトおよび‐ホスホネートの製造方法
US5134228A (en)	1992-07-28	Nucleoside-3'-phosphites for synthesis of oligonucleotides
US4503233A (en)	1985-03-05	Phosphorylating agent and process for the phosphorylation of organic hydroxyl compounds
Krawiecka et al.	1986	Reaction of thiolo and selenolo esters of phosphorus acids with halogens. 1. Stereochemical and phosphorus-31 NMR studies of reaction of S-methyl tert-butylphenylphosphinothiolate with elemental chlorine and sulfuryl chloride
CA2118153A1 (en)	1995-04-21	Use of nucleosides and nucleoside derivatives provided with enzymatically cleavable protecting groups in the synthesis of oligonucleotides
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