DK172064B1 - Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren - Google Patents

Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren Download PDF

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Publication number: DK172064B1
Authority: DK; Denmark
Prior art keywords: fusion protein; plasmid; amino acids; gene structure; amino acid
Prior art date: 1985-11-27

Application number

DK568586A

Other languages

Danish (da)

English (en)

Other versions

DK568586D0 (da

DK568586A (da

Inventor

Paul Habermann

Friedrich Wengenmayer

Original Assignee

Hoechst Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-11-27

Filing date

1986-11-26

Publication date

1997-10-06

1986-11-26 Application filed by Hoechst Ag filed Critical Hoechst Ag

1986-11-26 Publication of DK568586D0 publication Critical patent/DK568586D0/da

1987-05-28 Publication of DK568586A publication Critical patent/DK568586A/da

1997-10-06 Application granted granted Critical

1997-10-06 Publication of DK172064B1 publication Critical patent/DK172064B1/da

Links

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WPSKTVVMQCXPRO-BWBBJGPYSA-N Thr-Ser-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O WPSKTVVMQCXPRO-BWBBJGPYSA-N 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
LYERIXUFCYVFFX-GVXVVHGQSA-N Val-Leu-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N LYERIXUFCYVFFX-GVXVVHGQSA-N 0.000 description 1
238000007792 addition Methods 0.000 description 1
108010087924 alanylproline Proteins 0.000 description 1
230000004075 alteration Effects 0.000 description 1
108010077245 asparaginyl-proline Proteins 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
229960000074 biopharmaceutical Drugs 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
238000002144 chemical decomposition reaction Methods 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000000460 chlorine Substances 0.000 description 1
229910052801 chlorine Inorganic materials 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000012217 deletion Methods 0.000 description 1
FSXRLASFHBWESK-UHFFFAOYSA-N dipeptide phenylalanyl-tyrosine Natural products C=1C=C(O)C=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FSXRLASFHBWESK-UHFFFAOYSA-N 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000009483 enzymatic pathway Effects 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
102000013165 exonuclease Human genes 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
239000013604 expression vector Substances 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
108010025306 histidylleucine Proteins 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
230000006698 induction Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
108010054155 lysyllysine Proteins 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
238000005259 measurement Methods 0.000 description 1
239000002609 medium Substances 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
244000005700 microbiome Species 0.000 description 1
102000035118 modified proteins Human genes 0.000 description 1
108091005573 modified proteins Proteins 0.000 description 1
229930014626 natural product Natural products 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
239000006152 selective media Substances 0.000 description 1
230000000391 smoking effect Effects 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
238000010626 work up procedure Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Toxicology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Tropical Medicine & Parasitology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Endocrinology (AREA)
Microbiology (AREA)
Diabetes (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Materials For Medical Uses (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

DK568586A 1985-11-27 1986-11-26 Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren DK172064B1 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE19853541856 DE3541856A1 (de)	1985-11-27	1985-11-27	Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
DE3541856		1985-11-27

Publications (3)

Publication Number	Publication Date
DK568586D0 DK568586D0 (da)	1986-11-26
DK568586A DK568586A (da)	1987-05-28
DK172064B1 true DK172064B1 (da)	1997-10-06

Family

ID=6286938

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
DK568586A DK172064B1 (da)	1985-11-27	1986-11-26	Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren
DK052292A DK172210B1 (da)	1985-11-27	1992-04-21	Hirudinderivater

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
DK052292A DK172210B1 (da)	1985-11-27	1992-04-21	Hirudinderivater

Country Status (17)

Country	Link
EP (3)	EP0227938B1 (no)
JP (1)	JP2566933B2 (no)
KR (1)	KR950000300B1 (no)
AT (2)	ATE127841T1 (no)
AU (1)	AU595262B2 (no)
CA (1)	CA1341203C (no)
DE (4)	DE3541856A1 (no)
DK (2)	DK172064B1 (no)
ES (3)	ES2032378T3 (no)
FI (1)	FI93471C (no)
GR (1)	GR3005042T3 (no)
HU (1)	HU203579B (no)
IE (1)	IE59488B1 (no)
IL (1)	IL80755A0 (no)
NO (1)	NO176481C (no)
PT (1)	PT83813B (no)
ZA (1)	ZA868943B (no)

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US3125021A (en) *	1955-11-14	1964-03-17		Smooth
EP0168342B1 (de) *	1984-06-14	1991-07-03	Ciba-Geigy Ag	Verfahren zur Herstellung von Thrombin-Inhibitoren
DE3712985A1 (de) *	1987-04-16	1988-11-03	Hoechst Ag	Bifunktionelle proteine
DE3545568A1 (de) *	1985-12-21	1987-07-16	Hoechst Ag	Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung
DE3819079A1 (de) *	1988-06-04	1989-12-07	Hoechst Ag	Hirudin-derivate mit verzoegerter wirkung
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
DE3844211A1 (de) *	1988-12-29	1990-07-05	Hoechst Ag	Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
US5179196A (en) *	1989-05-04	1993-01-12	Sri International	Purification of proteins employing ctap-iii fusions
WO1991000912A1 (en) *	1989-07-07	1991-01-24	Massachusetts Institute Of Technology	Production and use of hybrid protease inhibitors
CU22222A1 (es) *	1989-08-03	1995-01-31	Cigb	Procedimiento para la expresion de proteinas heterologicas producidas de forma fusionada en escherichia coli, su uso, vectores de expresion y cepas recombinantes
GB8927722D0 (en) *	1989-12-07	1990-02-07	British Bio Technology	Proteins and nucleic acids
DE3942580A1 (de) *	1989-12-22	1991-06-27	Basf Ag	Verfahren zur herstellung von hirudin
US5270181A (en) *	1991-02-06	1993-12-14	Genetics Institute, Inc.	Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
DE4140381A1 (de) *	1991-12-07	1993-06-09	Hoechst Ag, 6230 Frankfurt, De	Neue synthetische isohirudine mit verbesserter stabilitaet
DE4404168A1 (de) *	1994-02-10	1995-08-17	Hoechst Ag	Hirudinderivate und Verfahren zu deren Herstellung
DE59711533D1 (de)	1996-07-26	2004-05-27	Aventis Pharma Gmbh	Insulinderivate mit erhöhter Zinkbindung
DE19726167B4 (de)	1997-06-20	2008-01-24	Sanofi-Aventis Deutschland Gmbh	Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung
DE19825447A1 (de)	1998-06-06	1999-12-09	Hoechst Marion Roussel De Gmbh	Neue Insulinanaloga mit erhöhter Zinkbildung
DE10033195A1 (de) *	2000-07-07	2002-03-21	Aventis Pharma Gmbh	Bifunktionale Fusionsproteine aus Hirudin und TAP
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10114178A1 (de)	2001-03-23	2002-10-10	Aventis Pharma Gmbh	Zinkfreie und zinkarme Insulinzubereitungen mit verbesserter Stabilität
DE10227232A1 (de)	2002-06-18	2004-01-15	Aventis Pharma Deutschland Gmbh	Saure Insulinzubereitungen mit verbesserter Stabilität
US9526764B2 (en)	2008-10-17	2016-12-27	Sanofi-Aventis Deutschland Gmbh	Combination of an insulin and a GLP-1-agonist
WO2011058082A1 (de)	2009-11-13	2011-05-19	Sanofi-Aventis Deutschland Gmbh	Pharmazeutische zusammensetzung umfassend einen glp-1-agonisten und methionin
HUE038147T2 (hu)	2009-11-13	2018-09-28	Sanofi Aventis Deutschland	GLP-1 agonistát, inzulint és metionint tartalmazó gyógyászati készítmény
WO2012028172A1 (en)	2010-08-30	2012-03-08	Sanofi-Aventis Deutschland Gmbh	Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2
US9821032B2 (en)	2011-05-13	2017-11-21	Sanofi-Aventis Deutschland Gmbh	Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
AR087693A1 (es)	2011-08-29	2014-04-09	Sanofi Aventis Deutschland	Combinacion farmaceutica para uso en el control glucemico en pacientes con diabetes de tipo 2
AR087744A1 (es)	2011-09-01	2014-04-16	Sanofi Aventis Deutschland	Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
HRP20230470T1 (hr)	2014-12-12	2023-07-21	Sanofi-Aventis Deutschland Gmbh	Formulacija fiksnog omjera inzulin glargin/liksisenatid
TWI748945B (zh)	2015-03-13	2021-12-11	德商賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患治療
TW201705975A (zh)	2015-03-18	2017-02-16	賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患之治療
KR20200080748A (ko)	2018-12-27	2020-07-07	주식회사 폴루스	음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
KR20200080747A (ko)	2018-12-27	2020-07-07	주식회사 폴루스	인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법

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Publication number	Priority date	Publication date	Assignee	Title
WO1985000817A1 (en) *	1983-08-10	1985-02-28	Amgen	Microbial expression of interleukin ii
DK108685A (da) *	1984-03-19	1985-09-20	Fujisawa Pharmaceutical Co	Vaekstfaktor i
CA1341417C (fr) *	1984-03-27	2003-01-21	Paul Tolstoshev	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
EP0158198A1 (en) *	1984-03-29	1985-10-16	Takeda Chemical Industries, Ltd.	DNA and use thereof
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3526995A1 (de) *	1985-07-27	1987-02-05	Hoechst Ag	Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung
US4865974A (en) *	1985-09-20	1989-09-12	Cetus Corporation	Bacterial methionine N-terminal peptidase
CA1297003C (en) *	1985-09-20	1992-03-10	Jack H. Nunberg	Composition and method for treating animals

1985
- 1985-11-27 DE DE19853541856 patent/DE3541856A1/de not_active Withdrawn
1986
- 1986-11-21 AT AT91114411T patent/ATE127841T1/de not_active IP Right Cessation
- 1986-11-21 EP EP86116140A patent/EP0227938B1/de not_active Expired - Lifetime
- 1986-11-21 DE DE3650322T patent/DE3650322D1/de not_active Expired - Lifetime
- 1986-11-21 DE DE8686116140T patent/DE3684892D1/de not_active Expired - Lifetime
- 1986-11-21 EP EP91114411A patent/EP0468539B1/de not_active Expired - Lifetime
- 1986-11-21 AT AT91114412T patent/ATE122397T1/de not_active IP Right Cessation
- 1986-11-21 ES ES198686116140T patent/ES2032378T3/es not_active Expired - Lifetime
- 1986-11-21 DE DE3650396T patent/DE3650396D1/de not_active Expired - Lifetime
- 1986-11-21 EP EP91114412A patent/EP0464867B1/de not_active Expired - Lifetime
- 1986-11-21 ES ES91114412T patent/ES2073081T3/es not_active Expired - Lifetime
- 1986-11-21 ES ES91114411T patent/ES2077747T3/es not_active Expired - Lifetime
- 1986-11-25 HU HU864872A patent/HU203579B/hu not_active IP Right Cessation
- 1986-11-25 IL IL80755A patent/IL80755A0/xx unknown
- 1986-11-25 FI FI864798A patent/FI93471C/fi not_active IP Right Cessation
- 1986-11-26 IE IE311986A patent/IE59488B1/en not_active IP Right Cessation
- 1986-11-26 CA CA000523857A patent/CA1341203C/en not_active Expired - Fee Related
- 1986-11-26 ZA ZA868943A patent/ZA868943B/xx unknown
- 1986-11-26 NO NO864759A patent/NO176481C/no unknown
- 1986-11-26 PT PT83813A patent/PT83813B/pt not_active IP Right Cessation
- 1986-11-26 KR KR1019860009990A patent/KR950000300B1/ko not_active Expired - Fee Related
- 1986-11-26 AU AU65693/86A patent/AU595262B2/en not_active Ceased
- 1986-11-26 JP JP61281621A patent/JP2566933B2/ja not_active Expired - Lifetime
- 1986-11-26 DK DK568586A patent/DK172064B1/da not_active IP Right Cessation
1992
- 1992-04-21 DK DK052292A patent/DK172210B1/da not_active IP Right Cessation
- 1992-06-26 GR GR920401111T patent/GR3005042T3/el unknown

Also Published As

Publication number	Publication date
EP0464867A1 (de)	1992-01-08
CA1341203C (en)	2001-03-13
EP0464867B1 (de)	1995-05-10
ES2032378T3 (es)	1993-02-16
IE59488B1 (en)	1994-03-09
FI93471B (fi)	1994-12-30
FI864798A0 (fi)	1986-11-25
DE3650322D1 (de)	1995-06-14
HUT43642A (en)	1987-11-30
FI93471C (fi)	1995-04-10
HU203579B (en)	1991-08-28
ATE122397T1 (de)	1995-05-15
ZA868943B (en)	1987-07-29
ES2073081T3 (es)	1995-08-01
KR870005098A (ko)	1987-06-04
AU6569386A (en)	1987-06-04
EP0468539A1 (de)	1992-01-29
DK172210B1 (da)	1998-01-05
DK568586D0 (da)	1986-11-26
AU595262B2 (en)	1990-03-29
DE3684892D1 (de)	1992-05-21
FI864798L (fi)	1987-05-28
KR950000300B1 (ko)	1995-01-13
DK52292D0 (da)	1992-04-21
EP0468539B1 (de)	1995-09-13
EP0227938A3 (en)	1988-11-23
PT83813A (de)	1986-12-01
ES2077747T3 (es)	1995-12-01
IL80755A0 (en)	1987-02-27
NO864759D0 (no)	1986-11-26
NO176481B (no)	1995-01-02
DK568586A (da)	1987-05-28
PT83813B (pt)	1989-06-30
DK52292A (da)	1992-04-21
ATE127841T1 (de)	1995-09-15
DE3650396D1 (de)	1995-10-19
JPS62143696A (ja)	1987-06-26
GR3005042T3 (no)	1993-05-24
NO176481C (no)	1995-04-12
JP2566933B2 (ja)	1996-12-25
EP0227938A2 (de)	1987-07-08
EP0227938B1 (de)	1992-04-15
DE3541856A1 (de)	1987-06-04
IE863119L (en)	1987-05-27

Publication	Publication Date	Title
DK172064B1 (da)	1997-10-06	Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren
DK172618B1 (da)	1999-03-01	Fusionsproteiner, deres fremstilling og anvendelse
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