[go: up one dir, main page]

DK171878B1 - Vektor med tilstrækkelig homologi til integration af DNA'et i kromosomalt DNA fra Yarrowia lipolytica, fremgangsmåde til fremstilling af den Y.lipolytica transformerende vektor og transformanter opnået derved - Google Patents

Vektor med tilstrækkelig homologi til integration af DNA'et i kromosomalt DNA fra Yarrowia lipolytica, fremgangsmåde til fremstilling af den Y.lipolytica transformerende vektor og transformanter opnået derved Download PDF

Info

Publication number
DK171878B1
DK171878B1 DK477084A DK477084A DK171878B1 DK 171878 B1 DK171878 B1 DK 171878B1 DK 477084 A DK477084 A DK 477084A DK 477084 A DK477084 A DK 477084A DK 171878 B1 DK171878 B1 DK 171878B1
Authority
DK
Denmark
Prior art keywords
lipolytica
dna
coli
vector
plasmid
Prior art date
Application number
DK477084A
Other languages
Danish (da)
English (en)
Other versions
DK477084A (da
DK477084D0 (da
Inventor
Lance Steven Davidow
John Robert Dezeeuw
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of DK477084D0 publication Critical patent/DK477084D0/da
Publication of DK477084A publication Critical patent/DK477084A/da
Application granted granted Critical
Publication of DK171878B1 publication Critical patent/DK171878B1/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Magnetic Ceramics (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Sludge (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Protection Of Transformers (AREA)
  • Electrotherapy Devices (AREA)
  • Blow-Moulding Or Thermoforming Of Plastics Or The Like (AREA)
  • Paper (AREA)
  • Air-Conditioning For Vehicles (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DK477084A 1983-10-06 1984-10-05 Vektor med tilstrækkelig homologi til integration af DNA'et i kromosomalt DNA fra Yarrowia lipolytica, fremgangsmåde til fremstilling af den Y.lipolytica transformerende vektor og transformanter opnået derved DK171878B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53959183A 1983-10-06 1983-10-06
US53959183 1983-10-06

Publications (3)

Publication Number Publication Date
DK477084D0 DK477084D0 (da) 1984-10-05
DK477084A DK477084A (da) 1985-05-15
DK171878B1 true DK171878B1 (da) 1997-07-28

Family

ID=24151875

Family Applications (2)

Application Number Title Priority Date Filing Date
DK477084A DK171878B1 (da) 1983-10-06 1984-10-05 Vektor med tilstrækkelig homologi til integration af DNA'et i kromosomalt DNA fra Yarrowia lipolytica, fremgangsmåde til fremstilling af den Y.lipolytica transformerende vektor og transformanter opnået derved
DK148195A DK171879B1 (da) 1983-10-06 1995-12-28 Fremgangsmåde til transformation af Yarrowia lipolytica og fremgangsmåde til kloning af gener ved komplementering af mutanter i Y.lipolytica

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK148195A DK171879B1 (da) 1983-10-06 1995-12-28 Fremgangsmåde til transformation af Yarrowia lipolytica og fremgangsmåde til kloning af gener ved komplementering af mutanter i Y.lipolytica

Country Status (17)

Country Link
EP (1) EP0138508B1 (xx)
JP (2) JP2559689B2 (xx)
KR (1) KR870000239B1 (xx)
AT (1) ATE73848T1 (xx)
AU (1) AU554118B2 (xx)
BR (1) BR8404984A (xx)
DE (1) DE3485589D1 (xx)
DK (2) DK171878B1 (xx)
ES (3) ES8605038A1 (xx)
FI (1) FI85987C (xx)
IE (1) IE57685B1 (xx)
IL (1) IL73119A (xx)
IN (1) IN163393B (xx)
NO (1) NO173743C (xx)
NZ (1) NZ209794A (xx)
PH (1) PH25363A (xx)
ZA (1) ZA847824B (xx)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2579617B2 (fr) * 1985-04-02 1987-06-26 Agronomique Inst Nat Rech Vecteurs de transformation de la levure yarrowia lipolytica, procede de transformation et levure transformee
EP0166659B1 (fr) * 1984-06-26 1989-08-30 Institut National De La Recherche Agronomique (Inra) Vecteurs de transformation de la levure yarrowia lipolytica, procédé de transformation et levure transformée
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
GB8529275D0 (en) * 1985-11-28 1986-01-02 Whitbread & Co Plc Dna recombination
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
FR2734843A1 (fr) * 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
GB9715905D0 (en) * 1997-07-28 1997-10-01 Ciba Geigy Ag Plant phosphoribosyl transferase and DNA coding thereof
FR2782733B1 (fr) 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
EP1492874B1 (en) * 2002-03-29 2011-01-19 XOMA Technology Ltd. Multigenic vector plasmids and methods for increasing expression of recombinant polypeptides
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
KR20090058077A (ko) * 2007-12-04 2009-06-09 충북대학교 산학협력단 생물체 정보의 염색체 내 표지 방법 및 그 정보가 표지된생물체
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
KR20110023862A (ko) * 2008-05-23 2011-03-08 시부스 오일즈, 엘엘씨 효모를 사용한 스쿠알렌 생산
WO2010135624A2 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
EP0138508B1 (en) 1992-03-18
DK171879B1 (da) 1997-07-28
NO173743B (no) 1993-10-18
ES8802399A1 (es) 1988-05-16
ES536546A0 (es) 1986-02-16
KR870000239B1 (ko) 1987-02-18
DK148195A (da) 1995-12-28
KR850003167A (ko) 1985-06-13
AU3387684A (en) 1985-04-18
JP2718659B2 (ja) 1998-02-25
AU554118B2 (en) 1986-08-07
EP0138508A1 (en) 1985-04-24
IL73119A (en) 1990-01-18
ES548941A0 (es) 1989-05-01
ES548940A0 (es) 1988-05-16
ZA847824B (en) 1986-05-28
ATE73848T1 (de) 1992-04-15
IE57685B1 (en) 1993-02-24
IE842535L (en) 1985-04-06
JPS60199386A (ja) 1985-10-08
PH25363A (en) 1991-05-13
ES8900255A1 (es) 1989-05-01
BR8404984A (pt) 1985-08-20
JP2559689B2 (ja) 1996-12-04
DK477084A (da) 1985-05-15
NO173743C (no) 1994-01-26
NZ209794A (en) 1989-01-27
IL73119A0 (en) 1984-12-31
NO844001L (no) 1985-04-09
FI85987C (fi) 1992-06-25
IN163393B (xx) 1988-09-17
ES8605038A1 (es) 1986-02-16
FI85987B (fi) 1992-03-13
DE3485589D1 (de) 1992-04-23
DK477084D0 (da) 1984-10-05
FI843932A0 (fi) 1984-10-05
FI843932L (fi) 1985-04-07
JPH09131176A (ja) 1997-05-20

Similar Documents

Publication Publication Date Title
US4880741A (en) Process for transformation of Yarrowia lipolytica
US5071764A (en) Process for integrative transformation of yarrowia lipolytica
DK171878B1 (da) Vektor med tilstrækkelig homologi til integration af DNA'et i kromosomalt DNA fra Yarrowia lipolytica, fremgangsmåde til fremstilling af den Y.lipolytica transformerende vektor og transformanter opnået derved
Clark-Adams et al. The SPT6 gene is essential for growth and is required for δ-mediated transcription in Saccharomyces cerevisiae
Greenleaf et al. Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.
Lahaye et al. PIF1: a DNA helicase in yeast mitochondria.
Craig et al. Mutations of the heat inducible 70 kilodalton genes of yeast confer temperature sensitive growth
Laloux et al. TEC1, a gene involved in the activation of Ty1 and Ty1-mediated gene expression in Saccharomyces cerevisiae: cloning and molecular analysis
Percival et al. Molecular cloning and primary structure of the uracil-DNA-glycosylase gene from Saccharomyces cerevisiae
Post-Beittenmiller et al. Regulation of basal and induced levels of the MEL1 transcript in Saccharomyces cerevisiae
Celenza et al. Structure and expression of the SNF1 gene of Saccharomyces cerevisiae
NO854333L (no) Isolering av gener fra gjaerarter av slekten pichia.
US5212087A (en) Ars sequence which is efficacious in yarrowia lipolytica
Yoo et al. Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae
Haas et al. Development of an integrative DNA transformation system for the yeast Candida tropicalis
Last et al. Isolation and characterization of the RNA2, RNA3, and RNA11 genes of Saccharomyces cerevisiae
Cohen et al. Organization of the MAL loci of Saccharomyces: physical identification and functional characterization of three genes at the MAL6 locus
Niederacher et al. Isolation and characterization of the regulatory HEX2 gene necessary for glucose repression in yeast
Tikhomirova et al. Evidence for autonomous replication and stabilization of recombinant plasmids in the transformants of yeast Hansenula polymorpha
WO1988010308A1 (en) Pheromone-inducible yeast promoter
US4628033A (en) Novel host strain for transformation of Yarrowia lipolytica
US4613572A (en) Yeast BAR1 gene plasmid
Marczak et al. Isolation of constitutive mutations affecting the proline utilization pathway in Saccharomyces cerevisiae and molecular analysis of the PUT3 transcriptional activator
Brisco et al. Cloning, disruption and chromosomal mapping of yeast LEU3, a putative regulatory gene
Soltyk et al. Isolation and characterization of the RNA2+, RNA4+, and RNA11+ genes of Saccharomyces cerevisiae

Legal Events

Date Code Title Description
PUP Patent expired