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DK1585822T3 - Promotorvarianter til ekspression af gener i en fungal celle - Google Patents

Promotorvarianter til ekspression af gener i en fungal celle

Info

Publication number
DK1585822T3
DK1585822T3 DK03789880.6T DK03789880T DK1585822T3 DK 1585822 T3 DK1585822 T3 DK 1585822T3 DK 03789880 T DK03789880 T DK 03789880T DK 1585822 T3 DK1585822 T3 DK 1585822T3
Authority
DK
Denmark
Prior art keywords
seq
biological substance
nucleic acid
promoter variants
fungal host
Prior art date
Application number
DK03789880.6T
Other languages
English (en)
Inventor
Debbie Yaver
Peter Nham
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK1585822T3 publication Critical patent/DK1585822T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04002—CTP synthase (6.3.4.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK03789880.6T 2002-11-18 2003-11-18 Promotorvarianter til ekspression af gener i en fungal celle DK1585822T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42731402P 2002-11-18 2002-11-18
PCT/US2003/037139 WO2004046373A2 (en) 2002-11-18 2003-11-18 Promoter variants for expressing genes in a fungal cell

Publications (1)

Publication Number Publication Date
DK1585822T3 true DK1585822T3 (da) 2012-05-14

Family

ID=32326518

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03789880.6T DK1585822T3 (da) 2002-11-18 2003-11-18 Promotorvarianter til ekspression af gener i en fungal celle

Country Status (8)

Country Link
US (6) US7368262B2 (da)
EP (1) EP1585822B1 (da)
JP (1) JP4490284B2 (da)
CN (2) CN100480391C (da)
AT (1) ATE542913T1 (da)
AU (1) AU2003294397A1 (da)
DK (1) DK1585822T3 (da)
WO (1) WO2004046373A2 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030228906A1 (en) 2002-04-19 2003-12-11 Walker Jay S. Methods and apparatus for providing communications services at a gaming machine
CN100480391C (zh) * 2002-11-18 2009-04-22 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
US7335106B2 (en) 2003-10-20 2008-02-26 Las Vegas Gaming, Inc. Closed-loop system for displaying promotional events and granting awards for electronic video games
WO2009052374A2 (en) * 2007-10-18 2009-04-23 Mayo Foundation For Medical Education And Research Igm-mediated receptor clustering and cell modulation
US8282480B2 (en) 2010-02-10 2012-10-09 Leap Forward Gaming Candle device for providing transaction verification on a gaming machine
US9245419B2 (en) 2010-02-10 2016-01-26 Leap Forward Gaming, Inc. Lottery games on an electronic gaming machine
US9240100B2 (en) 2010-02-10 2016-01-19 Leap Forward Gaming Virtual players card
US8088014B2 (en) 2010-02-10 2012-01-03 Leap Forward Gaming Gaming device and method for wireless gaming system providing non-intrusive processes
US8814681B2 (en) 2010-02-10 2014-08-26 Leap Forward Gaming, Inc. Candle device for generating display interfaces on the main display of a gaming machine
US8460091B2 (en) * 2010-02-10 2013-06-11 Leap Forward Gaming Remote power reset feature on a gaming machine
US8814706B2 (en) 2010-02-10 2014-08-26 Leap Forward Gaming, Inc. Radio candle mount
US8968086B2 (en) 2010-02-10 2015-03-03 Leap Forward Gaming, Inc. Video processing and signal routing apparatus for providing picture in a picture capabilities on an electronic gaming machine
WO2012075151A2 (en) * 2010-11-30 2012-06-07 Novozymes, Inc. Promoters for expressing genes in a fungal cell
BR112014004188A2 (pt) * 2011-08-24 2017-03-21 Novozymes Inc método para obter transformantes positivos de uma célula hospedeira fúngica filamentosa, célula hospedeira fúngica filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
JP5971661B2 (ja) * 2012-09-19 2016-08-17 国立大学法人 東京大学 神経活動依存的プロモータ活性を有するｄｎａ、及びこれを含むベクター
CN106636088B (zh) * 2016-09-22 2020-06-23 南京百斯杰生物工程有限公司 一种酵母上游激活元件在丝状真菌中的应用
JP7540709B2 (ja) 2020-11-30 2024-08-27 大関株式会社 糸状菌用プロモーターおよびその利用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
BR9408140A (pt) * 1993-11-24 1997-08-12 Monsanato Company Processo para controle de agentes patonênicos às plantas
PT765394E (pt) 1994-06-03 2002-03-28 Novozymes Biotech Inc Lacases myceliophthora purificadas e acidos nucleicos que as codificam
AU2705895A (en) * 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
CN1062017C (zh) * 1995-09-01 2001-02-14 中国科学院遗传研究所 防御素基因的植物表达载体质粒
JP3792725B2 (ja) 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
CN1190673A (zh) * 1996-04-29 1998-08-19 中国科学院微生物研究所 丝状真菌中独立复制的表达载体及表达系统
EP0966521A1 (en) 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
EP1078081B1 (en) 1998-05-20 2008-05-14 Novozymes, Inc. Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
JP5043254B2 (ja) 1998-10-26 2012-10-10 ノボザイムス アクティーゼルスカブ 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング
US6361973B1 (en) * 1999-03-22 2002-03-26 Novozymes Biotech, Inc. Promoters for expressing genes in a fungal cell
CN100532561C (zh) * 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
EP1235855A2 (en) 1999-03-22 2002-09-04 Novozymes Biotech, Inc. Methods for monitoring multiple gene expression
US6461837B1 (en) * 1999-11-30 2002-10-08 Novozymes Biotech, Inc. Methods for producing a polypeptide using a consensus translational initiator sequence
CN100480391C (zh) * 2002-11-18 2009-04-22 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
DE102006003576B4 (de) * 2006-01-25 2007-10-25 Tridelta Überspannungsableiter Gmbh Überspannungsableiter mit Käfig-Design

Also Published As

Publication number Publication date
US7368262B2 (en) 2008-05-06
CN101497908A (zh) 2009-08-05
US20170233751A1 (en) 2017-08-17
US9150856B2 (en) 2015-10-06
JP2006506095A (ja) 2006-02-23
EP1585822A4 (en) 2005-12-28
US20150376630A1 (en) 2015-12-31
US9873881B2 (en) 2018-01-23
US8138325B2 (en) 2012-03-20
AU2003294397A8 (en) 2004-06-15
AU2003294397A1 (en) 2004-06-15
US20040146975A1 (en) 2004-07-29
JP4490284B2 (ja) 2010-06-23
ATE542913T1 (de) 2012-02-15
WO2004046373A3 (en) 2005-02-10
EP1585822A2 (en) 2005-10-19
US20140099669A1 (en) 2014-04-10
US20080274500A1 (en) 2008-11-06
CN100480391C (zh) 2009-04-22
CN1780917A (zh) 2006-05-31
CN101497908B (zh) 2014-05-21
US20120190067A1 (en) 2012-07-26
US8604181B2 (en) 2013-12-10
EP1585822B1 (en) 2012-01-25
WO2004046373A2 (en) 2004-06-03
US9695429B2 (en) 2017-07-04

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