[go: up one dir, main page]

DK0843725T3 - Fremgangmåde til fremstilling af polypeptidvarianter - Google Patents

Fremgangmåde til fremstilling af polypeptidvarianter

Info

Publication number
DK0843725T3
DK0843725T3 DK96926325T DK96926325T DK0843725T3 DK 0843725 T3 DK0843725 T3 DK 0843725T3 DK 96926325 T DK96926325 T DK 96926325T DK 96926325 T DK96926325 T DK 96926325T DK 0843725 T3 DK0843725 T3 DK 0843725T3
Authority
DK
Denmark
Prior art keywords
polypeptide
dna
homologous
encoding
polypeptide variants
Prior art date
Application number
DK96926325T
Other languages
English (en)
Inventor
Jens Sigurd Okkels
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK0843725T3 publication Critical patent/DK0843725T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK96926325T 1995-08-11 1996-08-12 Fremgangmåde til fremstilling af polypeptidvarianter DK0843725T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK90795 1995-08-11
DK104795 1995-09-20
PCT/DK1996/000343 WO1997007205A1 (en) 1995-08-11 1996-08-12 Method for preparing polypeptide variants

Publications (1)

Publication Number Publication Date
DK0843725T3 true DK0843725T3 (da) 2002-08-12

Family

ID=26064882

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96926325T DK0843725T3 (da) 1995-08-11 1996-08-12 Fremgangmåde til fremstilling af polypeptidvarianter

Country Status (9)

Country Link
US (2) US20030148441A1 (da)
EP (2) EP1213350A3 (da)
JP (1) JP4156666B2 (da)
CN (1) CN1128875C (da)
AT (1) ATE216427T1 (da)
AU (2) AU6655496A (da)
DE (1) DE69620766T2 (da)
DK (1) DK0843725T3 (da)
WO (2) WO1997007205A1 (da)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
JPH08228778A (ja) * 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6489145B1 (en) * 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO1998031837A1 (en) 1997-01-17 1998-07-23 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
AU741834B2 (en) 1997-03-18 2001-12-13 Novozymes A/S An (in vitro) method for construction of a dna library
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
ATE272107T1 (de) 1997-04-09 2004-08-15 Danisco Lipase und verwendung davon zur verbesserung von teigen und backwaren
US6207442B1 (en) 1997-10-16 2001-03-27 Zymogenetics, Inc. Plasmid construction by homologous recombination
EP2270234B1 (en) 1997-12-08 2013-03-06 California Institute of Technology Method for creating polynucleotide and polypeptide sequences
JP2001526052A (ja) * 1997-12-12 2001-12-18 インタージェン カンパニー Ｆｒｅｔ標識オリゴヌクレオチドを用いたアポトーシスを検出する方法
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
EP0943678A3 (en) * 1998-02-18 2000-05-10 Novo Nordisk A/S Lipase variants
CA2325567A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
EP1104459A1 (en) 1998-08-12 2001-06-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
JP4283351B2 (ja) * 1998-08-27 2009-06-24 サントリー酒類株式会社 新規な油脂組成物の製造方法および用途
US6500639B2 (en) 1998-10-07 2002-12-31 Maxygen, Inc. DNA shuffling to produce nucleic acids for mycotoxin detoxification
US6767701B1 (en) 1998-10-26 2004-07-27 Novozymes A/S Methods of constructing and screening a DNA library of interest in filamentous fungal cells
CA2344619C (en) 1998-10-26 2012-01-03 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
AU1619400A (en) 1998-11-10 2000-05-29 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plantphenotypes
US7312062B2 (en) 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
EP2287298A1 (en) 1998-11-27 2011-02-23 Novozymes A/S Lipolytic enzyme variants
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
EP1108783A3 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide-mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
JP2003524394A (ja) 1999-02-11 2003-08-19 マキシジェン， インコーポレイテッド ハイスループット質量分析法
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
WO2000052155A2 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6569685B1 (en) 1999-10-05 2003-05-27 The Molecular Sciences Institute, Inc. Protein fingerprint system and related methods
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
CA2383965A1 (en) * 1999-10-13 2001-04-19 The Board Of Trustees Of The Leland Stanford Junior University Biosynthesis of polyketide synthase substrates
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
WO2001051510A2 (en) 2000-01-10 2001-07-19 Maxygen Holdings Ltd G-csf conjugates
SG121902A1 (en) 2000-01-11 2006-05-26 Maxygen Inc Integrated systems for diversity generation and screening
RU2278123C2 (ru) 2000-02-11 2006-06-20 Максиджен Холдингз Лтд. Молекулы, подобные фактору vii или viia
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
CA2411828A1 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
EP2287296A1 (en) 2000-06-26 2011-02-23 Novozymes A/S Lipolytic enzyme
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
WO2002010183A1 (en) 2000-07-31 2002-02-07 Menzel, Rolf Compositions and methods for directed gene assembly
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
EP1404827A2 (en) * 2001-02-23 2004-04-07 Novozymes A/S Lipolytic enzyme genes
ATE362315T1 (de) 2001-05-18 2007-06-15 Danisco Verfahren zur herstellung von teig mit einem enzym
DK2277997T3 (da) 2001-06-26 2013-12-02 Novozymes As Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
WO2003010183A2 (en) 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
EP1499739B1 (en) * 2002-04-22 2016-03-30 Novozymes, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
US20030199038A1 (en) * 2002-04-22 2003-10-23 Novozymes Biotech, Inc. Method for preparing polypeptide variants
CA2485506C (en) 2002-05-17 2012-02-28 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
US7560260B2 (en) 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
ATE494368T1 (de) 2002-10-01 2011-01-15 Novozymes As Polypeptide der gh-61-familie
US7348168B2 (en) 2002-12-20 2008-03-25 Novozymes A/S Polypeptides having cellobiohydrolase II activity and polynucleotides encoding same
ES2534903T3 (es) 2002-12-20 2015-04-30 Novozymes A/S Variantes de galactanasas
DE602004030000D1 (de) 2003-01-17 2010-12-23 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
DK1622921T3 (da) 2003-05-02 2010-09-13 Novozymes Inc Varianter af beta-glucosidaser
CN103088001A (zh) 2003-08-25 2013-05-08 诺维信股份有限公司 糖苷水解酶的变体
EP1673451B1 (en) 2003-10-10 2010-12-15 Novozymes A/S Protease variants
JP5059412B2 (ja) 2004-01-06 2012-10-24 ノボザイムス アクティーゼルスカブ アリシクロバシラスｓｐ．のポリペプチド
JP4891220B2 (ja) 2004-02-25 2012-03-07 ノボザイムス アクティーゼルスカブ 菌類細胞壁分解酵素
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EP1766040B1 (en) 2004-05-27 2017-08-09 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a dna library
CN102154391B (zh) 2004-07-16 2014-07-30 杜邦营养生物科学有限公司 脂肪分解酶及其在食品工业中的应用
US8119387B2 (en) 2005-08-16 2012-02-21 Novozymes A/S Polypeptides of strain Bacillus sp. P203
EP1777292A1 (de) * 2005-10-19 2007-04-25 Signalomics GmbH Verfahren zum Erzeugen von genetischer Diversität in vivo
CN103451164A (zh) * 2006-07-14 2013-12-18 诺维信股份有限公司 用于产生具有生物学活性的分泌型多肽的方法
CN101848985B (zh) 2007-11-05 2014-12-03 丹尼斯科美国公司 具有改变性质的杆菌物种TS-23的α-淀粉酶变体
KR20100085964A (ko) 2007-11-05 2010-07-29 다니스코 유에스 인크. 변경된 특성을 가진 알파-아밀라아제 변이체
EP2245130B1 (en) 2008-02-04 2020-11-18 Danisco US Inc. Ts23 alpha-amylase variants with altered properties
CN102224234B (zh) 2008-09-25 2014-04-16 丹尼斯科美国公司 α-淀粉酶混合物和使用所述混合物的方法
DK2414514T3 (da) 2009-04-01 2015-08-24 Danisco Us Inc Sammensætninger og fremgangsmåder omfattende alpha- amylasevarianter med ændrede egenskaber
BRPI0905122A2 (pt) * 2009-12-17 2011-08-09 Petroleo Brasileiro Sa processo de produção de lipases por meio de modificação genética de levedura
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
SG10201601324VA (en) * 2011-09-04 2016-03-30 Glytech Inc Glycosylated polypeptide and drug composition containing said polypeptide
EP3929289A1 (en) 2012-07-30 2021-12-29 Evolva, Inc. Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof
JP2015533285A (ja) 2012-11-01 2015-11-24 ザ・ユニバーシティ・オブ・ブリティッシュ・コロンビア シトクロムｐ４５０およびシトクロムｐ４５０レダクターゼポリペプチド、コードする核酸分子、ならびにそれらの用途
HUE035866T2 (en) 2013-03-14 2018-05-28 Evolva Inc Valencene synthase polypeptides, coding nucleic acid molecules and their use
CN107864658A (zh) 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
US20180073017A1 (en) 2015-04-07 2018-03-15 Novozymes A/S Methods for selecting enzymes having enhanced activity
EP3440207A1 (en) 2016-04-07 2019-02-13 Novozymes A/S Methods for selecting enzymes having protease activity
AU2017256768A1 (en) * 2016-04-29 2018-10-25 Puratos Nv Compositions for baked products containing lipolytic enzymes and uses thereof
CN108004151B (zh) * 2018-01-15 2019-07-19 青岛农业大学 一种从海带中分离的翅孢壳真菌及其应用
CN109666661A (zh) * 2018-12-31 2019-04-23 苏州埃斯腾特生物科技有限公司 去除纸浆中胶粘物的生物酶制剂
US20230287383A1 (en) 2020-07-03 2023-09-14 Engenes Biotech Gmbh PYRROLYSYL-tRNA SYNTHETASE VARIANTS AND USES THEREOF
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8315980D0 (en) * 1983-06-10 1983-07-13 Biogen Nv Producing hybrid dna sequences
EP0208491B1 (en) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination

Also Published As

Publication number Publication date
ATE216427T1 (de) 2002-05-15
DE69620766T2 (de) 2004-11-18
US20050124066A9 (en) 2005-06-09
EP1213350A3 (en) 2002-12-04
EP0843725B1 (en) 2002-04-17
WO1997007205A1 (en) 1997-02-27
EP1213350A2 (en) 2002-06-12
EP0843725A1 (en) 1998-05-27
WO1997007206A1 (en) 1997-02-27
DE69620766D1 (de) 2002-05-23
US20030148441A1 (en) 2003-08-07
JPH11510700A (ja) 1999-09-21
AU6655496A (en) 1997-03-12
US20050048649A1 (en) 2005-03-03
CN1128875C (zh) 2003-11-26
CN1192782A (zh) 1998-09-09
AU6655396A (en) 1997-03-12
JP4156666B2 (ja) 2008-09-24

Similar Documents

Publication Publication Date Title
DK0843725T3 (da) Fremgangmåde til fremstilling af polypeptidvarianter
ATE253633T1 (de) Cystic fibrosis gen
GB9701425D0 (en) A method for in vitro molecular evolution of protein function
DE69731834D1 (de) Agouti verwandtes gen
SG108208A1 (en) Connective tissue growth factor
AU3529189A (en) Modified baculovirus, method for preparing it and its use as an expression vector of genes
EP2258383A3 (en) Antigenic peptides derived from telomerase
NZ337676A (en) Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
FI107610B (fi) HCV-fuusioproteiini
SG49097A1 (en) Nucleotide sequences coding for a protein with urease activity
GB2143830A (en) Expression of plasmodium falciparum polypeptides from cloned cdna
PT94331A (pt) Processo de preparacao de uma citosina desaminase termicamente estavel
PT83968B (pt) Processo para melhoramento da expressao de proteina eucariotica, de preparacao de um plasmideo e plasmideo
NO177570B (no) Fremgangsmåte ved fremstilling av peptider med fibronektinbindende egenskaper, mikroorganisme og DNA-sekvens som koder for peptider med fibronektinbindende egenskaper
WO2000073323A3 (en) Adam polynucleotides and polypeptides
NZ335633A (en) Production of recombinant H. pylori polypeptides for treatment and diagnosis of H. pylori related diseases
EP1132402A4 (en) PEPTIDE FRAGMENTS WITH CELL DEATH PREVENTIVE ACTIVITY
WO1998027200A3 (en) Human and mammalian dna replication origin consensus sequences
AU7890687A (en) DNA sequence vectors, recombinant viruses and method which employs recombinant vaccinia viruses capable of multiplying in CHO cells
AU5327198A (en) Nucleotide sequence coding for a flavin-containing monooxygenase, corresponding protein and their applications in the fields of diagnosis and therapy
CA2194032A1 (en) Fused dna sequence, fused protein expressed from said fused dna sequence and method for expressing said fused protein
ES2115208T3 (es) Proteina de la superficie celular de brevibacterium lactofermentum.
WO1994026897A3 (en) Dna encoding two fish neuropeptides
WO1987007472A3 (en) A dna sequence encoding the mas oncogene, polypeptides encoded therefrom, and diagnostic and other methods based thereon
NZ298801A (en) Thrombolytic agent from Staphlococcus aureus