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DE69943326D1 - Neue chromogene Substrate zum Nachweis von bakteriellen Hydrolasen - Google Patents

Neue chromogene Substrate zum Nachweis von bakteriellen Hydrolasen

Info

Publication number
DE69943326D1
DE69943326D1 DE69943326T DE69943326T DE69943326D1 DE 69943326 D1 DE69943326 D1 DE 69943326D1 DE 69943326 T DE69943326 T DE 69943326T DE 69943326 T DE69943326 T DE 69943326T DE 69943326 D1 DE69943326 D1 DE 69943326D1
Authority
DE
Germany
Prior art keywords
nonchromogenic
compound
label
detecting
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69943326T
Other languages
English (en)
Inventor
Lyle Armstrong
Arthur James
Daniel Monget
Sylvain Orenga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of DE69943326D1 publication Critical patent/DE69943326D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/30Naphthol derivatives, e.g. alpha-naphthyl-esters, i.e. alpha-NE, beta-naphthyl-esters, i.e. beta-NE
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/924Hydrolases (3) acting on glycosyl compounds (3.2)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
DE69943326T 1998-04-02 1999-04-02 Neue chromogene Substrate zum Nachweis von bakteriellen Hydrolasen Expired - Lifetime DE69943326D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9804332A FR2777018B1 (fr) 1998-04-02 1998-04-02 Nouveau substrat chromogene ainsi que son procede d'utilisation

Publications (1)

Publication Number Publication Date
DE69943326D1 true DE69943326D1 (de) 2011-05-12

Family

ID=9524966

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69933759T Expired - Lifetime DE69933759T2 (de) 1998-04-02 1999-04-02 Neue chromogene substrate zum nachweis von bakteriellen hydrolasen
DE69943326T Expired - Lifetime DE69943326D1 (de) 1998-04-02 1999-04-02 Neue chromogene Substrate zum Nachweis von bakteriellen Hydrolasen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69933759T Expired - Lifetime DE69933759T2 (de) 1998-04-02 1999-04-02 Neue chromogene substrate zum nachweis von bakteriellen hydrolasen

Country Status (12)

Country Link
US (1) US6340573B1 (de)
EP (2) EP1066405B1 (de)
JP (1) JP4382985B2 (de)
AT (2) ATE503842T1 (de)
AU (1) AU753776B2 (de)
BR (1) BR9909356A (de)
CA (1) CA2325626C (de)
DE (2) DE69933759T2 (de)
ES (2) ES2275338T3 (de)
FR (1) FR2777018B1 (de)
PL (1) PL343524A1 (de)
WO (1) WO1999051767A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7839500A (en) * 1999-10-01 2001-05-10 Applied Imaging Corporation Simultaneous enzymatic detection of two analytes
FR2801903B1 (fr) * 1999-12-06 2004-01-30 Biomerieux Sa Nouveaux substrats enzymatiques pour la detection de pseudomonas aeruginosa, compositions les contenant et procede de detection de cette espece
EP1332357B1 (de) * 2000-10-14 2006-05-24 Alex M. Saunders Auf liganden basierendes lösungs-assay für niedrig konzentrierte analyten
FR2816956B1 (fr) * 2000-11-17 2004-08-20 Bio Merieux Procede et milieu de detection/identification de bacteries a activite esterasique
FR2903421B1 (fr) 2006-07-10 2008-10-03 Alain Rambach Milieu de culture solide pour la detection et/ou la discrimination au niveau de l'espece des enterocoques resistants aux glycopeptides
CA2681619C (en) 2006-12-19 2017-03-14 Becton, Dickinson And Company Chromogenic medium for the detection and identification of vancomycin resistant enterococci and method therfor
FR2926563B1 (fr) * 2008-01-21 2013-04-26 Biomerieux Sa Procede de detection et/ou d'identification de clostridium difficile
CN102977160B (zh) * 2012-11-15 2016-01-06 重庆医科大学 一种用4-硝基-1-萘酚或其衍生物为显色团制备β-半乳糖苷酶显色底物的方法及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018825B1 (de) * 1979-05-02 1985-02-13 National Research Development Corporation Verfahren zur Identifizierung von Bakterien und Reagenzsatz zur Verwendung in diesem Verfahren
DE3176589D1 (en) * 1980-12-02 1988-02-11 Health Lab Service Board Method for the estimation of n-acylated primary aromatic amines
US4588836A (en) * 1982-09-01 1986-05-13 Toyo Jozo Kabushiki Kaisha Novel synthetic substrate and assay method using the same
JPS5963198A (ja) * 1982-10-01 1984-04-10 Toyo Jozo Co Ltd 酵素を用いる定量法
JPS59220199A (ja) * 1983-03-07 1984-12-11 イ−−ワイ・ラボラトリ−ス・インコ−ポレ−テツド 吸収剤表面上の酵素基質を含む検査用品及びそれを用いる比色検査方法
GB8326696D0 (en) * 1983-10-05 1983-11-09 Health Lab Service Board Estimation of salicylates
EP0150227A1 (de) 1983-12-23 1985-08-07 FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. Dipeptidderivate und ihre Verwendung für die Bestimmung der Aktivität von Carboxypeptidasen
US4637979A (en) * 1984-04-06 1987-01-20 Miles Laboratories, Inc. Composition and test device for determining the presence of leukocytes containing a zwitterion coupling agent
DE4324392A1 (de) * 1993-07-21 1995-01-26 Merck Patent Gmbh Kulturmedium für den gleichzeitigen Nachweis von coliformen Bakterien und Escherichia coli
FR2708286B1 (fr) * 1993-07-28 1995-10-20 Rambach Alain Procédé d'identification de microorganismes avec au moins deux chromogènes.
US6387650B1 (en) * 1995-06-07 2002-05-14 Biocontrol Systems, Inc. Method and composition for detecting bacterial contamination in food products

Also Published As

Publication number Publication date
AU753776B2 (en) 2002-10-31
FR2777018A1 (fr) 1999-10-08
PL343524A1 (en) 2001-08-27
EP1754792A1 (de) 2007-02-21
EP1754792B1 (de) 2011-03-30
CA2325626A1 (fr) 1999-10-14
ES2275338T3 (es) 2007-06-01
ATE343640T1 (de) 2006-11-15
WO1999051767A1 (fr) 1999-10-14
CA2325626C (fr) 2010-03-23
FR2777018B1 (fr) 2003-04-25
DE69933759T2 (de) 2007-09-13
AU3040699A (en) 1999-10-25
ATE503842T1 (de) 2011-04-15
US6340573B1 (en) 2002-01-22
EP1066405A1 (de) 2001-01-10
BR9909356A (pt) 2000-12-12
ES2361038T3 (es) 2011-06-13
JP4382985B2 (ja) 2009-12-16
DE69933759D1 (de) 2006-12-07
EP1066405B1 (de) 2006-10-25
JP2002510503A (ja) 2002-04-09

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