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DE69834908D1 - Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen - Google Patents

Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen

Info

Publication number
DE69834908D1
DE69834908D1 DE69834908T DE69834908T DE69834908D1 DE 69834908 D1 DE69834908 D1 DE 69834908D1 DE 69834908 T DE69834908 T DE 69834908T DE 69834908 T DE69834908 T DE 69834908T DE 69834908 D1 DE69834908 D1 DE 69834908D1
Authority
DE
Germany
Prior art keywords
expression
plants
genes
plant
endogenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69834908T
Other languages
English (en)
Other versions
DE69834908T2 (de
Inventor
Vicki B Vance
James Carrington
Gail J Pruss
Laszlo Marton
William O Dawson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of South Carolina
University of South Carolina Aiken
Original Assignee
University of South Carolina
University of South Carolina Aiken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of South Carolina, University of South Carolina Aiken filed Critical University of South Carolina
Publication of DE69834908D1 publication Critical patent/DE69834908D1/de
Application granted granted Critical
Publication of DE69834908T2 publication Critical patent/DE69834908T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/34011—Potyviridae
    • C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69834908T 1997-03-28 1998-03-27 Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen Expired - Lifetime DE69834908T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/827,575 US5939541A (en) 1997-03-28 1997-03-28 Method for enhancing expression of a foreign or endogenous gene product in plants
PCT/US1998/006075 WO1998044097A2 (en) 1997-03-28 1998-03-27 Method for enhancing expression of a foreign or endogenous gene product in plants
US827575 2001-04-06

Publications (2)

Publication Number Publication Date
DE69834908D1 true DE69834908D1 (de) 2006-07-27
DE69834908T2 DE69834908T2 (de) 2007-02-01

Family

ID=25249575

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69834908T Expired - Lifetime DE69834908T2 (de) 1997-03-28 1998-03-27 Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen

Country Status (7)

Country Link
US (1) US5939541A (de)
EP (1) EP0970200B1 (de)
AT (1) ATE330007T1 (de)
AU (1) AU6871398A (de)
CA (1) CA2285026C (de)
DE (1) DE69834908T2 (de)
WO (1) WO1998044097A2 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395962B1 (en) * 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
WO2001034823A2 (en) * 1999-11-12 2001-05-17 University Of South Carolina Method for enhancing resistance in plants
KR20030004305A (ko) * 1999-11-22 2003-01-14 플랜트 바이오사이언스 리미티드 전사 후 유전자 사일런싱（ｐｔｇｓ）의 억제제와 함께발현시킴으로써 이식 유전자의 발현을 증가시키는 방법
ES2361925T3 (es) 2000-08-25 2011-06-24 Basf Plant Science Gmbh Polinucleótidos de plantas que codifican prenil proteasas.
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) * 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
US7414172B2 (en) 2001-03-28 2008-08-19 Consejo Superior De Investigaciones Cientificas (Csic) Method for improving plant tolerance to environmental stress
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
MXPA03009627A (es) 2001-04-24 2004-01-29 Cornell Res Foundation Inc MOLECULA SINTETICA DE ACIDO NUCLEICO PARA IMPARTIR CARACTERiSTICAS MULTIPLES.
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
US7001739B2 (en) * 2001-08-24 2006-02-21 The Texas A&M University System Isolation of proteins involved in posttranscriptional gene silencing and methods of use
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
JP2006503545A (ja) * 2002-02-14 2006-02-02 ラター， ウィリアム ジェイ． 処置される宿主における切断についてのキメラ分子
CA2492917C (en) * 2002-07-19 2011-10-18 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
WO2004044161A2 (en) * 2002-11-06 2004-05-27 Fraunhofer Usa Expression of foreign sequences in plants using trans-activation system
US8148608B2 (en) * 2004-02-20 2012-04-03 Fraunhofer Usa, Inc Systems and methods for clonal expression in plants
US7692063B2 (en) * 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7683238B2 (en) * 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
BRPI0409079A (pt) 2003-04-01 2006-04-18 Cropdesign Nv método para alteração das caracterìsticas de crescimento de plantas
JP2008537483A (ja) 2005-03-15 2008-09-18 イノジェネティックス・ナムローゼ・フェンノートシャップ ヌクレオシドアナログに対する感受性を低減したｂ型肝炎ウイルス変種およびその使用
WO2007005882A2 (en) * 2005-07-05 2007-01-11 North Carolina State University Methods and compositions for expressing proteins in plants
EP1981331A4 (de) * 2006-02-03 2009-09-09 Texas A & M Univ Sys Verbesserung der expression von mehrwertgenen durch transgene expression von tombusvirus-basierten p19-genmutanten
SI23374A (sl) 2010-05-24 2011-11-30 Nacionalni@inštitut@za@biologijo Uporaba glikozidaz in glikoziltransferaz za povečano proizvodnjo proteinov
FI20100248A0 (fi) * 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
CN103502455A (zh) 2011-01-17 2014-01-08 菲利普莫里斯生产公司 用于在植物中核酸表达的载体
BR112013018130A2 (pt) 2011-01-17 2017-06-27 Philip Morris Products Sa "método para produzir um polipeptídeo heterólogo em nicotiana tabacum, composição compreendendo um polipeptídeo por tal método e sistema para aplicação do mesmo"
EP2801619A1 (de) 2013-05-10 2014-11-12 Fondazione Edmund Mach Verfahren zur Erhöhung der Photosynthese mittels Polypeptid mit Curvature-Thylakoid-1 (CURT1)
BR112020004764A2 (pt) 2017-09-11 2020-09-24 R.J. Reynolds Tobacco Company métodos e composições para aumentar a expressão de genes de interesse em uma planta por coexpressão com p21

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6054684A (ja) * 1983-09-05 1985-03-29 Teijin Ltd 新規ｄνａ及びハイブリツドｄνａ
US5316931A (en) * 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5491076A (en) * 1993-11-01 1996-02-13 The Texas A&M University System Expression of foreign genes using a replicating polyprotein producing virus vector
WO1997004122A1 (en) * 1995-07-20 1997-02-06 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture

Also Published As

Publication number Publication date
EP0970200A2 (de) 2000-01-12
CA2285026A1 (en) 1998-10-08
AU6871398A (en) 1998-10-22
US5939541A (en) 1999-08-17
ATE330007T1 (de) 2006-07-15
WO1998044097A3 (en) 1998-12-30
DE69834908T2 (de) 2007-02-01
WO1998044097A2 (en) 1998-10-08
CA2285026C (en) 2011-03-01
EP0970200B1 (de) 2006-06-14

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