DE69730974D1 - Verfahren zur isolierung von nukleinsäuren unter anwendung von alkaliner phosphatase - Google Patents
Verfahren zur isolierung von nukleinsäuren unter anwendung von alkaliner phosphataseInfo
- Publication number
- DE69730974D1 DE69730974D1 DE69730974T DE69730974T DE69730974D1 DE 69730974 D1 DE69730974 D1 DE 69730974D1 DE 69730974 T DE69730974 T DE 69730974T DE 69730974 T DE69730974 T DE 69730974T DE 69730974 D1 DE69730974 D1 DE 69730974D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acids
- alkaline phosphatase
- isolating nucleic
- isolating
- phosphatase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000002260 Alkaline Phosphatase Human genes 0.000 title 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/681,922 US5981235A (en) | 1996-07-29 | 1996-07-29 | Methods for isolating nucleic acids using alkaline protease |
| US681922 | 1996-07-29 | ||
| PCT/US1997/013440 WO1998004730A1 (en) | 1996-07-29 | 1997-07-29 | Methods for isolating nucleic acids using alkaline protease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69730974D1 true DE69730974D1 (de) | 2004-11-04 |
| DE69730974T2 DE69730974T2 (de) | 2006-02-23 |
Family
ID=24737412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69730974T Expired - Lifetime DE69730974T2 (de) | 1996-07-29 | 1997-07-29 | Verfahren zur isolierung von nukleinsäuren unter anwendung von alkaliner phosphatase |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5981235A (de) |
| EP (1) | EP0918877B1 (de) |
| JP (1) | JP3943601B2 (de) |
| AU (1) | AU729932B2 (de) |
| CA (1) | CA2260940C (de) |
| DE (1) | DE69730974T2 (de) |
| WO (1) | WO1998004730A1 (de) |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0743949B1 (de) * | 1994-02-07 | 1999-12-15 | QIAGEN GmbH | Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie |
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| EP1036082B1 (de) * | 1997-12-06 | 2002-05-29 | DNA Research Innovations Limited | Isolierung von nukleinsäuren |
| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| US6194562B1 (en) * | 1998-04-22 | 2001-02-27 | Promega Corporation | Endotoxin reduction in nucleic acid purification |
| US6180408B1 (en) * | 1998-08-21 | 2001-01-30 | Washington University | Fluorescence polarization in nucleic acid analysis |
| EP1147226B1 (de) * | 1999-01-27 | 2013-01-23 | Folim G. Halaka | Materialien und verfahren zur reinigung von polyelektrolyten |
| WO2000063362A1 (en) * | 1999-04-21 | 2000-10-26 | Annovis, Inc. | Magnetic dna extraction kit for plants |
| US20020012990A1 (en) | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| DE10121463A1 (de) * | 2001-05-02 | 2003-02-27 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
| KR100454869B1 (ko) * | 2001-07-13 | 2004-11-03 | 주식회사 인트론바이오테크놀로지 | Ｄｎａ 추출용 세포 용해 버퍼 및 이를 이용한 ｄｎａ 추출방법 |
| DE10153957A1 (de) * | 2001-11-06 | 2003-05-22 | Quiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| US7208271B2 (en) | 2001-11-28 | 2007-04-24 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| EP1468116A2 (de) | 2002-01-16 | 2004-10-20 | Dynal Biotech ASA | Verfahren zur isolierung von nukleinsäuren und proteinen aus einer einzigen probe |
| US7364846B2 (en) * | 2002-10-11 | 2008-04-29 | Molecular Devices Corporation | Gene expression profiling from FFPE samples |
| AU2003298594A1 (en) * | 2002-11-01 | 2004-06-07 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
| WO2004108741A1 (en) * | 2003-06-04 | 2004-12-16 | Qiagen As | Process for the concentration and/or isolation of nucleic acid or nucleic acid-containing species |
| US20050164204A1 (en) * | 2004-01-27 | 2005-07-28 | Reed Thomas D. | Single use lyophilized rnase reagents, and kits and methods for using same |
| MXPA05001815A (es) * | 2004-02-20 | 2005-08-24 | Hoffmann La Roche | Adsorcion de acidos nucleicos a una fase solida. |
| KR100486179B1 (ko) * | 2004-03-02 | 2005-05-03 | 피경태 | 핵산을 분리하고 정제하기 위한 세포 용해 조성물, 방법 및 키트 |
| US7794932B2 (en) | 2004-04-16 | 2010-09-14 | Piotr Chomczynski | Reagents and methods for isolation of purified RNA |
| AU2006211216B2 (en) | 2005-01-31 | 2011-02-03 | Merck Sharp & Dohme Llc | Purification process for plasmid DNA |
| US20060188892A1 (en) * | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
| US20070015191A1 (en) * | 2005-07-01 | 2007-01-18 | Promega Corporation | Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification |
| EP1749892B1 (de) | 2005-08-02 | 2008-03-19 | Roche Diagnostics GmbH | Nukleotidsequenz zur Detektion von TSE |
| US7569367B2 (en) | 2005-09-07 | 2009-08-04 | Roche Diagnostics Operations, Inc. | Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy |
| WO2007070381A2 (en) * | 2005-12-09 | 2007-06-21 | Promega Corporation | Nucleic acid purification with a binding matrix |
| JP5354894B2 (ja) | 2006-12-11 | 2013-11-27 | エフ．ホフマン−ラ ロシュ アーゲー | ポリドカノールおよび誘導体を用いた核酸単離 |
| EP1932913B1 (de) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Nukleinsäureisolation mithilfe von Polidocanol und Derivaten davon |
| US8535888B2 (en) | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| CN101778938B (zh) * | 2007-08-20 | 2014-07-02 | 诺维信公司 | 降低核酸酶活性 |
| US8007205B2 (en) * | 2008-04-09 | 2011-08-30 | Marshall Iii Henry Bennie | Foundation wall vapor barrier system |
| EP2402462A1 (de) | 2008-06-06 | 2012-01-04 | F. Hoffmann-La Roche AG | Intern gesteuerte Multiplex-Erkennung und Quantifizierung von mikrobiellen Nukleinsäuren |
| CN101619355B (zh) * | 2009-07-02 | 2012-02-08 | 黑龙江大学 | 传统发酵豆酱中细菌的检测方法 |
| US8222397B2 (en) | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| WO2011026194A1 (en) * | 2009-09-07 | 2011-03-10 | Reproductive Health Science Pty Ltd | Nucleic acid extraction |
| ES2617258T5 (es) | 2010-07-29 | 2023-11-15 | Hoffmann La Roche | Preparación de muestras genéricas |
| EP2598656B1 (de) | 2010-07-29 | 2017-07-12 | F. Hoffmann-La Roche AG | Kontrollnukleinsäuren für mehrere parameter |
| ES2605303T3 (es) | 2010-07-29 | 2017-03-13 | F. Hoffmann-La Roche Ag | Detección cualitativa y cuantitativa de ácidos nucleicos microbianos |
| JP5972265B2 (ja) | 2010-07-29 | 2016-08-17 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ジェネリックｐｃｒ |
| EP2426222A1 (de) | 2010-09-07 | 2012-03-07 | F. Hoffmann-La Roche AG | Generischer Puffer zur Amplifikation |
| EP2465945A1 (de) | 2010-12-17 | 2012-06-20 | F. Hoffmann-La Roche AG | Allgemeine Matrix zur Steuerung von Nukleinsäuren |
| WO2013067069A2 (en) * | 2011-10-31 | 2013-05-10 | Leinweber Barbara | Dual digestion method for high yield nucleic acid recovery |
| US9057673B2 (en) | 2012-08-24 | 2015-06-16 | The Board Of Trustees Of The Leland Stanford Junior University | Method of preparing RNA from ribonuclease-rich sources |
| US20160010081A1 (en) * | 2014-07-11 | 2016-01-14 | Exact Sciences Corporation | Systems, methods and kits for extracting nucleic acid from digestive fluid |
| WO2016057891A1 (en) | 2014-10-09 | 2016-04-14 | Promega Corporation | Method of isolating nucleic acid |
| CN107257857B (zh) | 2014-11-14 | 2021-12-14 | 康宁股份有限公司 | 用于ivt后rna纯化的方法和试剂盒 |
| WO2016094465A1 (en) * | 2014-12-09 | 2016-06-16 | Corning Incorporated | Methods and kits for purifying plasmid dna |
| EP3303611B1 (de) | 2015-05-29 | 2019-10-02 | Roche Diagnostics GmbH | Verfahren zur inaktivierung von mikroben durch citraconsäureanhydrid |
| WO2017132630A1 (en) | 2016-01-29 | 2017-08-03 | Purigen Biosystems, Inc. | Isotachophoresis for purification of nucleic acids |
| CN111432919B (zh) | 2017-08-02 | 2022-08-02 | 普瑞珍生物系统公司 | 用于等速电泳的系统、设备和方法 |
| GB201803654D0 (en) * | 2018-03-07 | 2018-04-25 | Arcticzymes As | Thermolabile proteinases |
| KR20230139598A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| KR20230139597A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US4843155A (en) * | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5256557A (en) * | 1991-12-27 | 1993-10-26 | Solvay Enzymes, Inc. | Purified alkaline protease concentrate and method of preparation |
| US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| AU7129094A (en) * | 1993-07-08 | 1995-02-06 | Tepnel Medical Limited | Obtaining nucleic acids |
| ATE256735T1 (de) * | 1993-08-30 | 2004-01-15 | Promega Corp | Zusammensetzungen und verfahren zur reinigung von nukleinsäuren |
| US5646263A (en) * | 1994-09-19 | 1997-07-08 | Promega Corporation | High efficiency method for isolating target substances using a multisample separation device |
-
1996
- 1996-07-29 US US08/681,922 patent/US5981235A/en not_active Expired - Lifetime
-
1997
- 1997-07-29 AU AU38214/97A patent/AU729932B2/en not_active Ceased
- 1997-07-29 WO PCT/US1997/013440 patent/WO1998004730A1/en active IP Right Grant
- 1997-07-29 CA CA002260940A patent/CA2260940C/en not_active Expired - Fee Related
- 1997-07-29 EP EP97935224A patent/EP0918877B1/de not_active Expired - Lifetime
- 1997-07-29 JP JP50913998A patent/JP3943601B2/ja not_active Expired - Fee Related
- 1997-07-29 DE DE69730974T patent/DE69730974T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU3821497A (en) | 1998-02-20 |
| JP3943601B2 (ja) | 2007-07-11 |
| CA2260940C (en) | 2007-05-15 |
| EP0918877A1 (de) | 1999-06-02 |
| DE69730974T2 (de) | 2006-02-23 |
| CA2260940A1 (en) | 1998-02-05 |
| EP0918877A4 (de) | 2001-01-31 |
| EP0918877B1 (de) | 2004-09-29 |
| JP2000516091A (ja) | 2000-12-05 |
| US5981235A (en) | 1999-11-09 |
| WO1998004730A1 (en) | 1998-02-05 |
| AU729932B2 (en) | 2001-02-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |