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DE69225120D1 - Verfahren zum Lysieren von Mykobakterien - Google Patents

Verfahren zum Lysieren von Mykobakterien

Info

Publication number
DE69225120D1
DE69225120D1 DE69225120T DE69225120T DE69225120D1 DE 69225120 D1 DE69225120 D1 DE 69225120D1 DE 69225120 T DE69225120 T DE 69225120T DE 69225120 T DE69225120 T DE 69225120T DE 69225120 D1 DE69225120 D1 DE 69225120D1
Authority
DE
Germany
Prior art keywords
lysing mycobacteria
mycobacteria
lysing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69225120T
Other languages
English (en)
Other versions
DE69225120T2 (de
Inventor
Jillian Alyson Robson
Adriann Jeanelle Howard
William Edward Keating
James Arthur Down
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of DE69225120D1 publication Critical patent/DE69225120D1/de
Publication of DE69225120T2 publication Critical patent/DE69225120T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69225120T 1991-12-18 1992-11-25 Verfahren zum Lysieren von Mykobakterien Expired - Fee Related DE69225120T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US80980691A 1991-12-18 1991-12-18

Publications (2)

Publication Number Publication Date
DE69225120D1 true DE69225120D1 (de) 1998-05-20
DE69225120T2 DE69225120T2 (de) 1998-09-17

Family

ID=25202269

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69225120T Expired - Fee Related DE69225120T2 (de) 1991-12-18 1992-11-25 Verfahren zum Lysieren von Mykobakterien

Country Status (6)

Country Link
US (1) US5376527A (de)
EP (1) EP0547789B1 (de)
JP (1) JP2625340B2 (de)
AU (1) AU664050B2 (de)
CA (1) CA2084533C (de)
DE (1) DE69225120T2 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512440A (en) * 1991-12-18 1996-04-30 Becton Dickinson And Company Process for lysing Mycobacteria
CA2121659C (en) * 1993-05-11 2000-11-28 Michael C. Little Sample processing method for mycobacteria
IL110463A0 (en) * 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
AU682538B2 (en) * 1993-11-16 1997-10-09 Becton Dickinson & Company Process for lysing mycobacteria
KR100230909B1 (ko) * 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
AU2907995A (en) * 1994-06-23 1996-01-19 Dade International Inc. Method for the rapid isolation of nucleic acid
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor
US5707860A (en) * 1996-03-12 1998-01-13 Becton Dickinson And Company Vehicle for delivery of particles to a sample
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US6939696B1 (en) * 1998-08-03 2005-09-06 Becton, Dickinson And Company Cell disruption method using sonication
JP4358618B2 (ja) * 2001-07-19 2009-11-04 ジェネオーム サイエンシズ カナダ インコーポレイティド 核酸の放出及びそれらを検出するための細胞を迅速に溶解せしめるための万能な方法及び組成物
CN1656211B (zh) 2002-05-21 2010-04-28 爱科来株式会社 抗酸细菌的溶菌方法和用其进行基因扩增或检测的方法
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20050064490A1 (en) * 2003-09-23 2005-03-24 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
JP2009532512A (ja) 2006-02-28 2009-09-10 セルラー・バイオエンジニアリング・インコーポレイテッド 重合体組成物、ならびに汚染物を基板から除去するための方法
EP2027293B1 (de) * 2006-05-24 2014-11-19 Gen-Probe Incorporated Verfahren zum lysieren von mycobacterium und amplifikation seiner nukleinsäuren
EP2041315B1 (de) * 2006-06-19 2010-08-25 Becton, Dickinson and Company Verfahren und zusammensetzungen für den erhalt amplifizierbarer nukleinsäuren aus transportmedien ausgesetzten geweben, zellen oder viren
US20100099149A1 (en) * 2006-10-06 2010-04-22 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
JP2008167722A (ja) * 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
ATE505517T1 (de) * 2007-06-19 2011-04-15 Cellular Bioengineering Inc Verfahren zum schutz von substraten und zur entfernung von verunreinigungen aus solchen substraten
KR20100031505A (ko) * 2007-06-19 2010-03-22 셀룰라 바이오엔지니어링 인코포레이티드 미생물 및/또는 감염 인자를 처리하는 방법
US8158405B2 (en) * 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
EP2210936A1 (de) * 2009-01-27 2010-07-28 Curetis AG Verarbeitung und Analyse von viskosen, flüssigen biologischen Proben
JP5434199B2 (ja) * 2009-03-31 2014-03-05 東洋紡株式会社 抗酸菌遺伝子の増幅方法
DK2473596T3 (en) 2009-09-03 2018-01-22 Becton Dickinson Co METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS
JP5773280B2 (ja) * 2010-06-18 2015-09-02 東洋紡株式会社 抗酸菌の溶菌とその核酸の分離を同時に行う方法
WO2012177656A2 (en) 2011-06-19 2012-12-27 Abogen, Inc. Devices, solutions and methods for sample collection
EP2742510A1 (de) 2011-08-11 2014-06-18 Cellular Bioengineering, Inc. Polymerzusammensetzung

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5143736B2 (de) * 1971-10-06 1976-11-24
EP0041650A3 (de) * 1980-06-10 1982-05-12 Provesta Corporation Verfahren zur Verminderung des Nucleinsäuregehaltes in einzelligem Protein und Verfahren zur Herstellung eines einzelligen Proteinprodukts
ZA832976B (en) * 1982-05-05 1984-01-25 Phillips Petroleum Co A process for producing physiologically active protein
DD240909A1 (de) * 1983-12-09 1986-11-19 Adw Ddr Verfahren zur herstellung von mykobakterien-biomasse-lysat
CA1296604C (en) * 1986-03-20 1992-03-03 Kathleen Murphy Method for releasing rna and dna from cells
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
FR2651505B1 (fr) * 1989-09-06 1994-07-22 Pasteur Institut Fragments d'acides nucleiques derives d'un genome de mycobacterie appropriee, leurs applications dans le diagnostic des infections a mycobacteries et plasmides contenant lesdits fragments.
WO1991003558A1 (fr) * 1989-09-06 1991-03-21 Institut Pasteur Fragments d'acides nucleiques derives d'un genome de mycobacterie appropriee, leurs applications dans le diagnostic des infections a mycobacteries et plasmides contenant lesdits fragments
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Also Published As

Publication number Publication date
US5376527A (en) 1994-12-27
CA2084533A1 (en) 1993-06-19
DE69225120T2 (de) 1998-09-17
EP0547789A1 (de) 1993-06-23
CA2084533C (en) 1998-12-29
JPH06319527A (ja) 1994-11-22
EP0547789B1 (de) 1998-04-15
AU2844892A (en) 1993-06-24
JP2625340B2 (ja) 1997-07-02
AU664050B2 (en) 1995-11-02

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee