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DE69128520D1 - Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren - Google Patents

Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren

Info

Publication number
DE69128520D1
DE69128520D1 DE69128520T DE69128520T DE69128520D1 DE 69128520 D1 DE69128520 D1 DE 69128520D1 DE 69128520 T DE69128520 T DE 69128520T DE 69128520 T DE69128520 T DE 69128520T DE 69128520 D1 DE69128520 D1 DE 69128520D1
Authority
DE
Germany
Prior art keywords
quantification
detection
nucleic acids
target nucleic
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69128520T
Other languages
English (en)
Other versions
DE69128520T2 (de
Inventor
Yasutami Mitoma
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Application granted granted Critical
Publication of DE69128520D1 publication Critical patent/DE69128520D1/de
Publication of DE69128520T2 publication Critical patent/DE69128520T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE1991628520 1990-10-31 1991-10-31 Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren Expired - Lifetime DE69128520T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP29430590 1990-10-31

Publications (2)

Publication Number Publication Date
DE69128520D1 true DE69128520D1 (de) 1998-02-05
DE69128520T2 DE69128520T2 (de) 1998-07-09

Family

ID=17805976

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1991628520 Expired - Lifetime DE69128520T2 (de) 1990-10-31 1991-10-31 Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren

Country Status (3)

Country Link
EP (1) EP0487218B1 (de)
JP (1) JP2985446B2 (de)
DE (1) DE69128520T2 (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
CA2140877C (en) * 1992-07-24 2008-11-18 Raymond J. Harris Amplification and detection process
FR2694768B1 (fr) 1992-07-30 1994-12-23 Pasteur Institut Séquences d'oligonucléotides pour la détection spécifique de mollicutes par amplication de gènes conservés.
EP0599337B1 (de) * 1992-11-27 2006-03-08 Canon Kabushiki Kaisha Verfahren und Sonde zum Nachweis von Nukleinsäuren
JP3247001B2 (ja) * 1992-12-21 2002-01-15 キヤノン株式会社 ピリリウム化合物を用いた2本鎖核酸の検出方法、ピリリウム化合物を含有するプローブ及びそれを用いた標的核酸の検出方法、新規ピリリウム化合物
JP3451667B2 (ja) * 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
CA2126391C (en) * 1993-09-13 2002-01-08 Nobuko Yamamoto Determination of nucleic acid by pcr, measurement of number of microbial cells, genes, or gene-copies by pcr, and measuring-kit employed for the same
US6043032A (en) * 1993-09-22 2000-03-28 Tosoh Corporation Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB2282138B (en) * 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
JPH07233065A (ja) 1993-12-27 1995-09-05 Canon Inc ピリリウム塩又はピリリウム類似塩を含む光化学治療薬
ATE280243T1 (de) * 1994-04-29 2004-11-15 Johnson & Johnson Clin Diag Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits
EP0684239B1 (de) * 1994-05-26 2003-12-10 Canon Kabushiki Kaisha Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
JP3189000B2 (ja) * 1994-12-01 2001-07-16 東ソー株式会社 特定核酸配列の検出方法
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
WO1997007235A2 (en) * 1995-08-14 1997-02-27 Abbott Laboratories All-in-one nucleic acid amplification assay
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
EP1179600B1 (de) * 1996-06-04 2005-05-11 University Of Utah Research Foundation Überwachung der Hybridisierung während PCR
EP0837141B1 (de) 1996-10-03 2003-01-08 Canon Kabushiki Kaisha Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse
JP3968810B2 (ja) * 1997-01-24 2007-08-29 東ソー株式会社 核酸配列分析方法
AU6534898A (en) * 1997-02-14 1998-09-08 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
EP0882801A1 (de) * 1997-06-03 1998-12-09 Institut Pasteur De Lille Verfahren für die Selektion von cDNA Fragmente
US6403313B1 (en) 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
DK1206571T3 (da) 1999-05-04 2004-12-06 Ortho Clinical Diagnostics Inc Hurtig og effektiv indfangning af DNA fra pröve uden anvendelse af cellelyseringsreagens
WO2000075371A1 (fr) * 1999-06-04 2000-12-14 Tosoh Corporation Procede d'amplification d'acide nucleique potentialise
US6265170B1 (en) 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
US7267945B2 (en) 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
AU2003235894A1 (en) * 2002-05-08 2003-12-12 Arkray, Inc. Method of detecting target nucleic acid
AU2003298658A1 (en) 2002-11-15 2004-06-15 Idenix (Cayman) Limited 2'-branched nucleosides and flaviviridae mutation
JP2008180677A (ja) * 2007-01-26 2008-08-07 Konica Minolta Medical & Graphic Inc マイクロチップ検査システム、マイクロチップ検査装置及びプログラム
EP2185726B1 (de) 2007-08-06 2014-01-08 Orion Genomics, LLC Neue einzelnukleotid-polymorphismen und kombinationen neuer und bekannter polymorphismen zur bestimmung der allelspezifischen expression des igf2-gens
EP2913398B1 (de) 2007-08-27 2017-07-12 Life Technologies Corporation Verfahren für PCR
CA2639416C (en) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Diagnostic test for susceptibility to b-raf kinase inhibitors
JP2011506926A (ja) 2007-12-06 2011-03-03 エージェンシー フォー サイエンス, テクノロジー アンド リサーチ 化学反応を実施しモニターするための一体型装置
JP5049404B2 (ja) 2008-03-31 2012-10-17 エージェンシー フォー サイエンス, テクノロジー アンド リサーチ 相互接続マルチチャンバデバイスを使用する流体処理および移送の方法
EP2396429B1 (de) 2009-02-11 2015-05-27 Orion Genomics, LLC Kombinationen aus polymorphismen zur bestimmung von allelspezifischer igf2-expression
CN102356161A (zh) 2009-03-19 2012-02-15 株式会社钟化 核酸的检测方法以及试剂盒、装置
EP2789693B1 (de) 2009-08-13 2017-10-04 Life Technologies Corporation Amelogenin-SNP auf X-Chromosomen
EP2488668B1 (de) 2009-10-15 2023-07-26 Life Technologies Corporation Neue menschliche einzelnukleotid-polymorphismen
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
ES2647612T3 (es) 2010-06-04 2017-12-22 Chronix Biomedical Biomarcadores de ácidos nucleicos en circulación asociados al cáncer de próstata
EP2937423A1 (de) 2010-09-21 2015-10-28 Life Technologies Corporation Se33-mutationen mit auswirkung auf die genotypkonkordanz
SG188978A1 (en) 2010-09-22 2013-05-31 Kaneka Corp Method and device for detecting nucleic acid, and kit
KR101904787B1 (ko) 2011-03-04 2018-10-08 가부시키가이샤 가네카 핵산의 검출 방법 및 그 방법에 사용하는 디바이스, 키트
RU2014110270A (ru) 2011-08-31 2015-10-10 Ф. Хоффманн-Ля Рош Аг Способ прогнозирования риска гипертензии, связанной с антиангиогенной терапией
RU2014110271A (ru) 2011-08-31 2015-10-10 Ф. Хоффманн-Ля Рош Аг Восприимчивость к ингибиторам ангиогенеза
CA2846630A1 (en) 2011-09-19 2013-03-28 Genentech, Inc. Combination treatments comprising c-met antagonists and b-raf antagonists
ES2729554T3 (es) 2011-10-21 2019-11-04 Chronix Biomedical Biomarcadores de ácidos nucleicos en circulación asociados al cáncer colorrectal
SG11201402554SA (en) 2011-11-23 2014-06-27 Hoffmann La Roche Responsiveness to angiogenesis inhibitors
US10294529B2 (en) 2012-04-10 2019-05-21 Life Sciences Research Partners Vzw Microsatellite instability markers in detection of cancer
US10633707B2 (en) 2012-04-10 2020-04-28 Vib Vzw Markers for detecting microsatellite instability in cancer and determining synthetic lethality with inhibition of the DNA base excision repair pathway
US10077474B2 (en) 2012-05-29 2018-09-18 Abbott Molecular, Inc. Method of designing primers, method of detecting single nucleotide polymorphisms (SNPs), method of distinguishing SNPs, and related primers, detectable oligonucleotides, and kits
WO2014150300A2 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Method for amplification and assay of rna fusion gene variants, method of distinguishing same and related primers, probes, and kits
CA2901293A1 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Detection of bisulfite converted nucleotide sequences
EP3572093A1 (de) 2014-03-31 2019-11-27 Debiopharm International SA Fgfr-fusionen
EP3218515B1 (de) 2014-11-10 2023-04-26 F. Hoffmann-La Roche AG Therapeutische und diagnostische verfahren für il-33-vermittelte erkrankungen
EP3356555B1 (de) * 2015-09-29 2021-07-07 Life Technologies Corporation Systeme und verfahren zur ausführung von digitaler pcr
US10844441B2 (en) 2016-05-16 2020-11-24 Life Technologies Corporation Penta e polymorphisms for human identification
JP7275148B2 (ja) * 2018-01-05 2023-05-17 ストラトス ゲノミクス インコーポレイテッド 副溝結合成分による核酸重合の強化
EP3749362A1 (de) 2018-02-09 2020-12-16 F. Hoffmann-La Roche AG Therapeutische und diagnostische verfahren für mastzellvermittelte entzündliche erkrankungen
EP4118236A1 (de) 2020-03-13 2023-01-18 MedImmune Limited Therapeutische verfahren zur behandlung von patienten mit risikoalellen in il33

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
ES8706823A1 (es) * 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
BE1000572A4 (fr) * 1987-05-20 1989-02-07 Block Myron J Cellule rta, appareil et procede pour titrer un polynucleotide dans un liquide.
AU5838690A (en) * 1989-06-12 1991-01-08 Compagnie Oris Industrie S.A. Method for detecting specific nucleic acid sequences and applications of same

Also Published As

Publication number Publication date
DE69128520T2 (de) 1998-07-09
JPH05237000A (ja) 1993-09-17
EP0487218A1 (de) 1992-05-27
EP0487218B1 (de) 1997-12-29
JP2985446B2 (ja) 1999-11-29

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