DE60332775D1 - Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen - Google Patents
Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von SekundärstrukturenInfo
- Publication number
- DE60332775D1 DE60332775D1 DE60332775T DE60332775T DE60332775D1 DE 60332775 D1 DE60332775 D1 DE 60332775D1 DE 60332775 T DE60332775 T DE 60332775T DE 60332775 T DE60332775 T DE 60332775T DE 60332775 D1 DE60332775 D1 DE 60332775D1
- Authority
- DE
- Germany
- Prior art keywords
- secondary structure
- dual
- sequence
- forming region
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000003556 assay Methods 0.000 title 1
- 238000009396 hybridization Methods 0.000 title 1
- 239000000523 sample Substances 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41226302P | 2002-09-20 | 2002-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60332775D1 true DE60332775D1 (de) | 2010-07-08 |
Family
ID=32326255
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60332775T Expired - Lifetime DE60332775D1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen |
| DE60309817T Expired - Lifetime DE60309817T2 (de) | 2002-09-20 | 2003-09-18 | Mehrzweck-Primer und -Sonden für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60309817T Expired - Lifetime DE60309817T2 (de) | 2002-09-20 | 2003-09-18 | Mehrzweck-Primer und -Sonden für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8394944B2 (de) |
| EP (2) | EP1428892B1 (de) |
| JP (1) | JP2004113240A (de) |
| AT (2) | ATE469243T1 (de) |
| DE (2) | DE60332775D1 (de) |
| ES (1) | ES2276016T3 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005084277A2 (en) * | 2004-02-27 | 2005-09-15 | University Of Denver | Method of isolating nucleic acid targets |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| CA2606723A1 (en) | 2005-05-09 | 2006-11-16 | Panomics, Inc. | Multiplex capture of nucleic acids |
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| WO2006124771A2 (en) | 2005-05-12 | 2006-11-23 | Panomics, Inc. | Multiplex branched-chain dna assays |
| EP1910572B1 (de) | 2005-06-20 | 2015-12-30 | Advanced Cell Diagnostics, Inc. | Verfahren zum nachweis von nukleinsäuren in einzelnen zellen und zur identifizierung seltener zellen in grossen heterogenen zellpopulationen |
| WO2007044427A2 (en) | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| EP1951905B1 (de) | 2005-11-10 | 2012-04-11 | Affymetrix, Inc. | Nachweis von nukleinsäuren über amplifikation von stellvertreternukleinsäuren |
| EP2630260B1 (de) | 2010-10-21 | 2015-11-18 | Advanced Cell Diagnostics, Inc. | Hochsensitives verfahren für den in-situ-nachweis von nukleinsäuren |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| US11085073B2 (en) | 2015-04-24 | 2021-08-10 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| EP3286331B1 (de) * | 2015-04-24 | 2022-12-14 | Qiagen GmbH | Verfahren zur hybridisierung eines nukleinsäuremoleküls |
| ES2893100T3 (es) | 2015-10-12 | 2022-02-08 | Advanced Cell Diagnostics Inc | Detección in situ de variantes de nucleótidos en muestras con un elevado nivel de ruido, y composiciones y métodos relacionados con ésta |
| JP6730525B2 (ja) | 2016-11-21 | 2020-07-29 | ナノストリング テクノロジーズ，インコーポレイティド | 化学組成物とそれを利用する方法 |
| US10844430B2 (en) * | 2018-01-24 | 2020-11-24 | Qiagen Sciences, Llc | DNA sequencing reaction additive |
| SG11202011274YA (en) | 2018-05-14 | 2020-12-30 | Nanostring Technologies Inc | Chemical compositions and methods of using same |
| CN119173642A (zh) * | 2022-06-20 | 2024-12-20 | 株式会社日立高新技术 | 引物、dna检测方法和dna检测试剂盒 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU602875B2 (en) * | 1985-12-18 | 1990-11-01 | British Technology Group Limited | Newcastle disease virus gene clones |
| SE454781B (sv) * | 1986-10-17 | 1988-05-30 | Wallac Oy | Hybridiseringsforfarande for detektion av polynukleotidsekvens |
| US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5359100A (en) * | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5030557A (en) * | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| GB8920097D0 (en) * | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| ES2103756T3 (es) * | 1990-06-22 | 1997-10-01 | Hoffmann La Roche | Deteccion de metabolizadores ineficaces de farmacos. |
| US5849481A (en) * | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| ES2082256T3 (es) * | 1992-03-23 | 1996-03-16 | Hoffmann La Roche | Metodo de deteccion del adn. |
| US5827648A (en) * | 1995-12-13 | 1998-10-27 | Chiron Corporation | Differential hybridization for relative quantification of variant populations |
| US6187538B1 (en) * | 1993-12-13 | 2001-02-13 | Bayer Corporation | Differential hybridization for relative quantification of variant populations |
| CA2186465A1 (en) * | 1994-04-04 | 1995-10-12 | Richard A. Martinelli | Hybridization-ligation assays for the detection of specific nucleic acid sequences |
| US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5622822A (en) * | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
| US5747248A (en) * | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| CA2239287C (en) * | 1995-12-05 | 2008-02-26 | Jorn Erland Koch | A cascade nucleic acid amplification reaction |
| US6139800A (en) * | 1997-06-23 | 2000-10-31 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
| EP1009852A2 (de) * | 1997-09-04 | 2000-06-21 | Bayer Corporation | Oligonukleotidsonden mit löschbaren fluoreszierende labels, und verfahren zur herstellung davon |
| US6136568A (en) * | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
| US6054568A (en) * | 1998-01-16 | 2000-04-25 | The Perkin-Elmer Corporation | Nucleobase oligomers |
| CA2318779C (en) * | 1998-01-22 | 2009-08-25 | Luminex Corporation | Microparticles with multiple fluorescent signals |
| JP2002500897A (ja) * | 1998-01-27 | 2002-01-15 | クリニカル・マイクロ・センサーズ・インコーポレイテッド | 電子的核酸検出の増幅 |
| JP4196236B2 (ja) * | 1998-03-17 | 2008-12-17 | 東洋紡績株式会社 | 核酸増幅用試薬および配列特異的な核酸増幅法 |
| AU3996299A (en) * | 1998-05-14 | 1999-11-29 | Luminex Corporation | Diode laser based measurement apparatus |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6287778B1 (en) * | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
| WO2001083826A2 (en) * | 2000-05-03 | 2001-11-08 | Massachusetts Institute Of Technology | Methods and reagents for assembling molecules on solid supports |
| US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| EP1412526A2 (de) | 2001-06-29 | 2004-04-28 | Syngenta Participations AG | Verfahren zur verbesserten detektion und unterschiedung von differenzieller genexpression mittels enzymatischer ligation von proben und amplifizierung |
-
2003
- 2003-09-15 US US10/667,191 patent/US8394944B2/en active Active
- 2003-09-18 AT AT06017482T patent/ATE469243T1/de not_active IP Right Cessation
- 2003-09-18 ES ES03255861T patent/ES2276016T3/es not_active Expired - Lifetime
- 2003-09-18 DE DE60332775T patent/DE60332775D1/de not_active Expired - Lifetime
- 2003-09-18 EP EP03255861A patent/EP1428892B1/de not_active Expired - Lifetime
- 2003-09-18 AT AT03255861T patent/ATE346169T1/de not_active IP Right Cessation
- 2003-09-18 DE DE60309817T patent/DE60309817T2/de not_active Expired - Lifetime
- 2003-09-18 EP EP06017482A patent/EP1785491B1/de not_active Expired - Lifetime
- 2003-09-19 JP JP2003329188A patent/JP2004113240A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1428892B1 (de) | 2006-11-22 |
| DE60309817T2 (de) | 2007-09-13 |
| JP2004113240A (ja) | 2004-04-15 |
| EP1785491A1 (de) | 2007-05-16 |
| ES2276016T3 (es) | 2007-06-16 |
| US8394944B2 (en) | 2013-03-12 |
| EP1785491B1 (de) | 2010-05-26 |
| ATE469243T1 (de) | 2010-06-15 |
| US20040132061A1 (en) | 2004-07-08 |
| EP1428892A1 (de) | 2004-06-16 |
| DE60309817D1 (de) | 2007-01-04 |
| ATE346169T1 (de) | 2006-12-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |