DE602004021778D1 - Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren - Google Patents
Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäurenInfo
- Publication number
- DE602004021778D1 DE602004021778D1 DE602004021778T DE602004021778T DE602004021778D1 DE 602004021778 D1 DE602004021778 D1 DE 602004021778D1 DE 602004021778 T DE602004021778 T DE 602004021778T DE 602004021778 T DE602004021778 T DE 602004021778T DE 602004021778 D1 DE602004021778 D1 DE 602004021778D1
- Authority
- DE
- Germany
- Prior art keywords
- nitrilases
- acid sequences
- nucleic acid
- carboxylic acids
- expression constructs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010033272 Nitrilase Proteins 0.000 title abstract 5
- 150000001735 carboxylic acids Chemical class 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 abstract 4
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 241000588813 Alcaligenes faecalis Species 0.000 abstract 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract 1
- 229940005347 alcaligenes faecalis Drugs 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 150000002825 nitriles Chemical class 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45047003P | 2003-02-27 | 2003-02-27 | |
| PCT/EP2004/001804 WO2004076655A1 (en) | 2003-02-27 | 2004-02-24 | Modified nitrilases and their use in methods for the production of carboxylic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602004021778D1 true DE602004021778D1 (de) | 2009-08-13 |
Family
ID=32927657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004021778T Expired - Lifetime DE602004021778D1 (de) | 2003-02-27 | 2004-02-24 | Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7985572B2 (de) |
| EP (1) | EP1599584B1 (de) |
| JP (1) | JP4584242B2 (de) |
| CN (1) | CN100445375C (de) |
| AT (1) | ATE435278T1 (de) |
| AU (1) | AU2004215238B2 (de) |
| DE (1) | DE602004021778D1 (de) |
| WO (1) | WO2004076655A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT412092B (de) * | 2003-02-27 | 2004-09-27 | Dsm Fine Chem Austria Gmbh | Verfahren zur herstellung chiraler alpha-hydroxycarbonsäuren durch enzymatische hydrolyse von chiralen cyanhydrinen |
| CN101341251A (zh) * | 2005-12-20 | 2009-01-07 | 巴斯夫欧洲公司 | 由5-降冰片烯-2-甲腈使用芳基乙腈水解酶制备5-降冰片烯-2-甲酸的方法 |
| CA2868393A1 (en) * | 2012-04-02 | 2013-10-10 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of oncology-related proteins and peptides |
| US9878056B2 (en) | 2012-04-02 | 2018-01-30 | Modernatx, Inc. | Modified polynucleotides for the production of cosmetic proteins and peptides |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| CN103898083B (zh) * | 2014-04-21 | 2016-06-29 | 武汉大学 | 一种新型水解酶超家族酰胺酶Azl13及其制备与应用 |
| US9804036B2 (en) * | 2014-06-19 | 2017-10-31 | Infineon Technologies Ag | Temperature sensor calibration |
| CN105130792A (zh) * | 2015-09-21 | 2015-12-09 | 上海佰瑞生物科技有限公司 | 一种对由生物法合成的(r)-(-)-扁桃酸进行分离纯化的方法 |
| CN105349583A (zh) * | 2015-12-23 | 2016-02-24 | 武汉武药制药有限公司 | 一种酶法制备(r)-邻氯扁桃酸的方法及应用 |
| CN106854673B (zh) * | 2016-12-23 | 2020-05-15 | 枣庄市杰诺生物酶有限公司 | 利用腈水解酶工程菌制备r-邻氯扁桃酸的方法 |
| CN113195727A (zh) | 2018-10-18 | 2021-07-30 | 巴斯夫欧洲公司 | 用于产生丙烯酸或其盐的方法 |
| CN110205315B (zh) * | 2019-05-30 | 2021-01-01 | 中国石油大学(华东) | 腈水解酶XiNit2及其编码基因和应用 |
| CN111471668B (zh) * | 2020-02-28 | 2022-05-24 | 浙江工业大学 | 一种腈水解酶突变体及其在制备1-氰基环己基乙酸中的应用 |
| CN113106130B (zh) * | 2020-06-23 | 2023-03-14 | 核芯生物医药科技(杭州)有限公司 | 聚集性葡萄球菌来源的腈水合酶在制备酰胺吡嗪类化合物中的应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU209143B (en) | 1986-01-08 | 1994-03-28 | Rhone Poulenc Agrochimie | Method for producing nitrilase specific to halogenated benzonitryl, sequences containing its coding gene and cells containing said sequences |
| EP0332379B1 (de) | 1988-03-08 | 1996-08-14 | Japan Energy Corporation | Verfahren zur Herstellung von L-alpha-Aminosäuren |
| US5283193A (en) * | 1988-06-27 | 1994-02-01 | Asahi Kasei Kogyo K.K. | Process for producing optically active α-substituted organic acid and microorganism and enzyme used therefor |
| DK314989A (da) | 1988-06-27 | 1989-12-28 | Asahi Chemical Ind | Fremgangsmaade til fremstilling af optisk aktive alfa-substituerede organiske syrer, samt mikroorganismer og enzymer anvendelige ved fremgangsmaaden |
| DE69131217T2 (de) | 1990-03-30 | 1999-09-23 | Mitsubishi Rayon Co., Ltd. | Verfahren zur Herstellung von R(-)Mandelsäure und deren Derivaten |
| JP2974737B2 (ja) | 1990-08-16 | 1999-11-10 | 三菱レイヨン株式会社 | 光学活性乳酸の製造法 |
| ATE134706T1 (de) | 1990-09-20 | 1996-03-15 | Du Pont | Verfahren zur herstellung von 2- alkansäureenantiomeren |
| JP2676568B2 (ja) | 1991-06-26 | 1997-11-17 | 日東化学工業株式会社 | Ｒ（−）−マンデル酸およびその誘導体の製造法 |
| JP3218133B2 (ja) | 1993-02-03 | 2001-10-15 | 三菱レイヨン株式会社 | フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法 |
| JP2720140B2 (ja) | 1993-02-03 | 1998-02-25 | 日東化学工業株式会社 | フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法 |
| JP3354688B2 (ja) | 1994-01-28 | 2002-12-09 | 三菱レイヨン株式会社 | 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法 |
| NZ292559A (en) * | 1994-09-22 | 1998-03-25 | Rhone Poulec Nutrition Animale | Preparation of racemic alpha-substituted 4-methylthiobutyric acids by enzymatic hydrolysis |
| JP3154633B2 (ja) * | 1994-12-28 | 2001-04-09 | 三菱レイヨン株式会社 | ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子 |
| JPH0937788A (ja) | 1995-07-31 | 1997-02-10 | Nitto Chem Ind Co Ltd | 新規なニトリラーゼ遺伝子 |
| EP0974669B1 (de) | 1996-02-29 | 2007-05-02 | Nippon Soda Co., Ltd. | Prozess für die herstellung von alpha-hydroxysäuren mit hilfe eines mikroorganismus und eines neuen mikroorganismus. |
| FR2780416B1 (fr) | 1998-06-10 | 2002-12-20 | Rhone Poulenc Nutrition Animal | Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede |
| DE19848129A1 (de) * | 1998-10-19 | 2000-04-20 | Basf Ag | Verfahren zur Herstellung chiraler Carbonsäuren aus Nitrilen mit Hilfe einer Nitrilase oder Mikroorganismen, die ein Gen für die Nitrilase enthalten |
| CA2356741C (en) | 1999-10-26 | 2011-01-25 | Showa Denko K.K. | Novel rhodococcus bacterium, nitrilase gene, nitryl hydratase gene and amidase gene from rhodococcus bacterium, and process for producing carboxylic acids using them |
| FR2800749B1 (fr) | 1999-11-08 | 2004-07-16 | Rhone Poulenc Animal Nutrition | Procede de modulation de la selective des nitrilases, nouvelles nitrilases obtenues par ce procede et leur utilisation |
| US7300775B2 (en) | 1999-12-29 | 2007-11-27 | Verenium Corporation | Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents |
| DE10010149A1 (de) * | 2000-03-03 | 2001-09-06 | Basf Ag | Nitrilase aus Rhodococcus rhodochrous NCIMB 11216 |
| WO2002029079A2 (en) | 2000-10-04 | 2002-04-11 | Maxygen, Inc. | Enantioselective production of amino carboxylic acids |
| AU2002320264B2 (en) | 2001-06-05 | 2008-05-01 | Exelixis, Inc. | GFATs as modifiers of the p53 pathway and methods of use |
| DK2327766T3 (en) | 2001-06-21 | 2016-03-07 | Basf Enzymes Llc | nitrilases |
| US7932064B2 (en) | 2002-06-13 | 2011-04-26 | Verenium Corporation | Processes for making (R)-ethyl 4-cyano-3-hydroxybutyric acid |
-
2004
- 2004-02-24 AT AT04713882T patent/ATE435278T1/de active
- 2004-02-24 JP JP2006501937A patent/JP4584242B2/ja not_active Expired - Lifetime
- 2004-02-24 US US10/546,611 patent/US7985572B2/en active Active
- 2004-02-24 AU AU2004215238A patent/AU2004215238B2/en not_active Ceased
- 2004-02-24 DE DE602004021778T patent/DE602004021778D1/de not_active Expired - Lifetime
- 2004-02-24 EP EP04713882A patent/EP1599584B1/de not_active Expired - Lifetime
- 2004-02-24 WO PCT/EP2004/001804 patent/WO2004076655A1/en active Application Filing
- 2004-02-24 CN CNB2004800053978A patent/CN100445375C/zh not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7985572B2 (en) | 2011-07-26 |
| JP2006519002A (ja) | 2006-08-24 |
| AU2004215238A1 (en) | 2004-09-10 |
| CN100445375C (zh) | 2008-12-24 |
| EP1599584A1 (de) | 2005-11-30 |
| ATE435278T1 (de) | 2009-07-15 |
| EP1599584B1 (de) | 2009-07-01 |
| CN1753990A (zh) | 2006-03-29 |
| AU2004215238B2 (en) | 2009-08-27 |
| JP4584242B2 (ja) | 2010-11-17 |
| WO2004076655A1 (en) | 2004-09-10 |
| US20060259999A1 (en) | 2006-11-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |