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DE60133111D1 - Verfahren zur erkennung und zum assay von nukleinsäuresequenzen - Google Patents

Verfahren zur erkennung und zum assay von nukleinsäuresequenzen

Info

Publication number
DE60133111D1
DE60133111D1 DE60133111T DE60133111T DE60133111D1 DE 60133111 D1 DE60133111 D1 DE 60133111D1 DE 60133111 T DE60133111 T DE 60133111T DE 60133111 T DE60133111 T DE 60133111T DE 60133111 D1 DE60133111 D1 DE 60133111D1
Authority
DE
Germany
Prior art keywords
detecting
nucleic acid
acid sequences
assaying nucleic
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60133111T
Other languages
English (en)
Other versions
DE60133111T2 (de
Inventor
Robert C Getts
James M Kadushin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genisphere LLC
Original Assignee
Genisphere Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genisphere Inc filed Critical Genisphere Inc
Application granted granted Critical
Publication of DE60133111D1 publication Critical patent/DE60133111D1/de
Publication of DE60133111T2 publication Critical patent/DE60133111T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60133111T 2000-07-19 2001-07-19 Verfahren zur erkennung und zum assay von nukleinsäuresequenzen Expired - Lifetime DE60133111T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21939700P 2000-07-19 2000-07-19
US219397P 2000-07-19
PCT/US2001/022818 WO2002006511A2 (en) 2000-07-19 2001-07-19 Methods for detecting and assaying nucleic acid sequences

Publications (2)

Publication Number Publication Date
DE60133111D1 true DE60133111D1 (de) 2008-04-17
DE60133111T2 DE60133111T2 (de) 2009-03-12

Family

ID=22819100

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60133111T Expired - Lifetime DE60133111T2 (de) 2000-07-19 2001-07-19 Verfahren zur erkennung und zum assay von nukleinsäuresequenzen

Country Status (7)

Country Link
US (1) US20020072060A1 (de)
EP (1) EP1319179B1 (de)
JP (1) JP2004506179A (de)
AT (1) ATE388402T1 (de)
AU (1) AU2001282920A1 (de)
DE (1) DE60133111T2 (de)
WO (1) WO2002006511A2 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000663A2 (en) 1999-06-28 2001-01-04 Oklahoma Medical Research Foundation Catalytically active recombinant memapsin and methods of use thereof
US20020051981A1 (en) * 2000-03-08 2002-05-02 Robert Getts Methods for assay and detection on a microarray
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US20040265883A1 (en) * 2003-06-27 2004-12-30 Biocept, Inc. mRNA expression analysis
US20050084880A1 (en) * 2003-07-11 2005-04-21 Ronald Duman Systems and methods for diagnosing & treating psychological and behavioral conditions
US7731906B2 (en) 2003-07-31 2010-06-08 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US20060035251A1 (en) * 2004-06-30 2006-02-16 Whitehead Institute For Biomedical Research Novel methods for high-throughput genome-wide location analysis
CN1312293C (zh) * 2004-11-08 2007-04-25 博奥生物有限公司 一种高通量生物芯片及其应用
US20070048741A1 (en) * 2005-08-24 2007-03-01 Getts Robert C Methods and kits for sense RNA synthesis
US20070105124A1 (en) * 2005-11-08 2007-05-10 Getts Robert C Methods and kits for nucleic acid amplification
EP3088083B1 (de) 2006-03-24 2018-08-01 Handylab, Inc. Methode zur durchführung von pcr mittels einer kartusche mit mehreren bahnen
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
EP2164989B1 (de) * 2007-05-23 2018-01-24 The Trustees Of The University Of Pennsylvania Trägerstoffe als ziele für intrazelluläre arzneistoffzuführung
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
JP5651011B2 (ja) 2007-07-13 2015-01-07 ハンディーラブ インコーポレイテッド ポリヌクレオチド捕捉材料およびその使用方法
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
ES2617599T3 (es) 2011-04-15 2017-06-19 Becton, Dickinson And Company Termociclador microfluídico de exploración en tiempo real y métodos para termociclado sincronizado y detección óptica de exploración
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
KR102121853B1 (ko) 2011-09-30 2020-06-12 벡톤 디킨슨 앤드 컴퍼니 일체화된 시약 스트립
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
BR112014018995B1 (pt) 2012-02-03 2021-01-19 Becton, Dickson And Company sistemas para executar ensaio automatizado
US9651549B2 (en) * 2012-07-13 2017-05-16 Genisphere, Llc Lateral flow assays using DNA dendrimers
EP2976109B1 (de) 2013-03-21 2018-07-25 Genisphere, LLC Zelluläre freisetzung von dna-interkalierenden wirkstoffen

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE23752T1 (de) * 1980-08-21 1986-12-15 Oriel Scient Ltd Optische analyseeinrichtung.
US5175270A (en) * 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US6361940B1 (en) * 1996-09-24 2002-03-26 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization and priming specificity
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
EP1005573B1 (de) * 1997-07-29 2006-05-24 Polyprobe, Inc. Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
KR20020007332A (ko) * 1999-03-18 2002-01-26 추후제출 일 단계 샘플 제조 및 복합적인 생물학적 샘플에서 핵산의검출
US20020051981A1 (en) * 2000-03-08 2002-05-02 Robert Getts Methods for assay and detection on a microarray
US20020094538A1 (en) * 2001-01-13 2002-07-18 Getts Robert C. Methods for detecting and assaying nucleic acid sequences using temperature cycling
US20040009487A1 (en) * 2001-08-31 2004-01-15 Kadushin James M. Methods for blocking nonspecific hybridizations of nucleic acid sequences
WO2003012147A1 (en) * 2001-02-20 2003-02-13 Datascope Investment Corp. Method for reusing standard blots and microarrays utilizing dna dendrimer technology

Also Published As

Publication number Publication date
EP1319179A2 (de) 2003-06-18
DE60133111T2 (de) 2009-03-12
WO2002006511A2 (en) 2002-01-24
AU2001282920A1 (en) 2002-01-30
WO2002006511A3 (en) 2002-04-11
JP2004506179A (ja) 2004-02-26
ATE388402T1 (de) 2008-03-15
US20020072060A1 (en) 2002-06-13
EP1319179B1 (de) 2008-03-05
EP1319179A4 (de) 2005-01-19

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GENISPHERE, LLC., HATFIELD, PA., US