[go: up one dir, main page]

DE60035459D1 - Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation - Google Patents

Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation

Info

Publication number
DE60035459D1
DE60035459D1 DE60035459T DE60035459T DE60035459D1 DE 60035459 D1 DE60035459 D1 DE 60035459D1 DE 60035459 T DE60035459 T DE 60035459T DE 60035459 T DE60035459 T DE 60035459T DE 60035459 D1 DE60035459 D1 DE 60035459D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid amplification
time analysis
automated real
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60035459T
Other languages
English (en)
Other versions
DE60035459T2 (de
Inventor
Carl T Wittwer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Utah Research Foundation Inc filed Critical University of Utah Research Foundation Inc
Application granted granted Critical
Publication of DE60035459D1 publication Critical patent/DE60035459D1/de
Publication of DE60035459T2 publication Critical patent/DE60035459T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
DE60035459T 1999-04-27 2000-04-26 Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation Expired - Lifetime DE60035459T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13125699P 1999-04-27 1999-04-27
US131256P 1999-04-27
US09/391,811 US6387621B1 (en) 1999-04-27 1999-09-08 Automated analysis of real-time nucleic acid amplification
US391811 1999-09-08

Publications (2)

Publication Number Publication Date
DE60035459D1 true DE60035459D1 (de) 2007-08-23
DE60035459T2 DE60035459T2 (de) 2008-03-20

Family

ID=26829286

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60035459T Expired - Lifetime DE60035459T2 (de) 1999-04-27 2000-04-26 Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation

Country Status (5)

Country Link
US (2) US6387621B1 (de)
EP (1) EP1059523B1 (de)
JP (1) JP4689004B2 (de)
AT (1) ATE366821T1 (de)
DE (1) DE60035459T2 (de)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
EP1290219B1 (de) * 2000-03-27 2005-06-01 Applera Corporation Verbesserter invasionsassay
DE60228952D1 (de) * 2001-05-07 2008-10-30 Mayo Foundation Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
US7188030B2 (en) * 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
CA2400601A1 (en) * 2001-08-31 2003-02-28 University Of Utah Research Foundation Real-time gene quantification with internal standards
US7373253B2 (en) * 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
EP1335028B1 (de) * 2002-02-12 2008-01-16 University of Utah Research Foundation Multi-Test-Analyse von Echzeit-Nukleinsäureamplifikationen
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
WO2003072805A2 (en) 2002-02-21 2003-09-04 Asm Scientific, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CN100519761C (zh) 2002-03-01 2009-07-29 拉瓦格恩公司 基因组变异的快速分析
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
AUPS267802A0 (en) * 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
US7083974B2 (en) * 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
CA2499360A1 (en) * 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
US7074598B2 (en) * 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
JP2006512928A (ja) * 2002-11-15 2006-04-20 ジェン−プローブ・インコーポレーテッド 炭疽菌(Bacillusanthracis)核酸を検出するためのアッセイおよび組成物
SG173221A1 (en) * 2003-02-28 2011-08-29 Ravgen Inc Methods for detection of genetic disorders
US20040185446A1 (en) * 2003-03-18 2004-09-23 Jones Alison M. Cpn60 targets for quantification of microbial species
EP1606419A1 (de) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systeme und methoden zur erhohung der milchproduktion in tieren
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20050202484A1 (en) 2004-02-19 2005-09-15 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
KR100738073B1 (ko) 2004-09-01 2007-07-12 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 초기 핵산 농도를정량화하는 방법
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
AU2006223223B2 (en) 2005-03-10 2012-04-12 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
EP1897016B1 (de) * 2005-06-22 2012-08-01 Gen-Probe Incorporated Verfahren und algorithmus zum quantifizieren von polynukleotiden
CA2616241C (en) 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
EP1798542A1 (de) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytisches Verfahren und Gerät
RU2294532C1 (ru) * 2006-02-14 2007-02-27 Закрытое акционерное общество "Научно-производственная фирма ДНК-Технология" Способ стандартизации данных полимерной цепной реакции с регистрацией накопления продуктов реакции по флуоресценции непосредственно во время реакции (пцр "в реальном времени")
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
CA2650993C (en) 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
JP4993260B2 (ja) * 2006-05-15 2012-08-08 石川県 遺伝子定量装置及び定量方法
US7802215B2 (en) * 2006-06-06 2010-09-21 Fujitsu Limited System and method for providing an improved sliding window scheme for clock mesh analysis
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8005628B2 (en) * 2007-01-29 2011-08-23 Applied Biosystems, Llc Systems and methods for baseline correction using non-linear normalization
CN101688841B (zh) * 2007-06-29 2011-12-07 霍夫曼-拉罗奇有限公司 用于确定pcr和其他数据集中的串扰系数的系统和方法
US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
US8386184B2 (en) * 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
US8380457B2 (en) * 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
ES2424759T3 (es) * 2007-11-14 2013-10-08 Grifols Therapeutics Inc. Procedimiento de ajuste de resultados de un instrumento de reacción en cadena de la polimerasa (PCR)
KR101413659B1 (ko) 2007-12-06 2014-07-01 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 시료 중의 표적 핵산의초기 농도를 결정하는 방법
EP2233587A4 (de) * 2007-12-26 2014-02-26 Arkray Inc Beurteilungsverfahren der Amplifikation von Nukleinsäuren und Vorrichtung zur Beurteilung der Amplifikation von Nukleinsäuren
US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US8271205B2 (en) * 2008-09-19 2012-09-18 Corbett Research Pty Ltd Method and system for analysis of melt curves, particularly dsDNA and protein melt curves
US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
GB0905325D0 (en) 2009-03-30 2009-05-13 Selex Sensors & Airborne Sys Detection system
WO2010135310A1 (en) * 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP3360974B1 (de) 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Reagentien zur rekombinase polymerase amplifikation
WO2011017567A1 (en) * 2009-08-05 2011-02-10 Life Technologies Corporation Methods for the analysis of proximity binding assay data
US8023794B2 (en) * 2009-08-10 2011-09-20 The Boeing Company Apparatus and method for establishing an optical path spanning a discontinuity in an optical channel
EP2491144B1 (de) 2009-10-21 2015-09-30 Brandeis University Verfahren zur analyse einzelsträngiger nukleinsäuresequenzen
RU2423685C1 (ru) * 2010-05-20 2011-07-10 Александр Иванович Заико Способ стандартизации данных конвекционной полимерной цепной реакции с регистрацией накопления продуктов реакций по флуоресценции непосредственно во время реакции
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
CN103476940B (zh) 2011-04-07 2016-07-13 美艾利尔圣地亚哥有限公司 重组聚合酶扩增混合物的监测
DK2756101T3 (en) 2011-09-15 2018-08-27 David A Shafer PROBLEMS: ANTISON DETAIL COMPOSITIONS FOR DETECTING HIGH OR SPECIFIC DNA OR RNA
AU2013203281A1 (en) 2012-02-03 2013-08-22 Axxin Pty Ltd Nucleic acid amplification and detection apparatus and method
AU2015275310B2 (en) * 2012-02-03 2018-10-18 Axxin Pty Ltd Nucleic acid amplification and detection apparatus and method
US20130273547A1 (en) * 2012-04-16 2013-10-17 Samsung Techwin Co., Ltd. Method to determine and correct baseline and to characterize pcr amplification kinetics
EP2864503B1 (de) 2012-06-26 2019-10-09 Axxin Pty Ltd Nukleinsäureamplifikations- und -nachweiskit
US9932628B2 (en) 2012-07-27 2018-04-03 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
EP3967773A1 (de) 2012-09-10 2022-03-16 BioFire Diagnostics, LLC Erkennung von mehrfachen verstärkungszyklen
US9714447B2 (en) 2013-08-19 2017-07-25 General Electric Company Detection of nucleic acid amplification in a porous substrate
US10463290B2 (en) 2014-11-14 2019-11-05 Axxin Pty Ltd. Biological sample collection and storage assembly
AU2016295422B2 (en) 2015-07-17 2022-01-06 Axxin Pty Ltd Diagnostic test assembly, apparatus, method
US10328147B2 (en) 2017-03-24 2019-06-25 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Herpes simplex virus type-1(HSV-1) vaccine strain VC2 generating an anti-EHV-1 immune response
EP3655549B1 (de) 2017-07-21 2023-01-04 BioFire Diagnostics, LLC Detektion unter verwendung von gleichzeitigen schmelzkurven
CN111386465B (zh) 2017-09-27 2024-03-22 艾可辛私人有限公司 诊断测试系统和方法
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3895170A4 (de) * 2018-12-14 2022-10-12 Seegene, Inc. Verfahren zum nachweis eines zielanalyten in einer probe unter verwendung einer s-förmigen funktion für einen anstiegsdatensatz
ES2969861T3 (es) * 2019-12-20 2024-05-23 Euroimmun Medizinische Labordiagnostika Ag Procedimiento y dispositivo para la evaluación cualitativa de datos de PCR en tiempo real
CN111426661B (zh) * 2020-04-09 2022-10-18 基蛋生物科技股份有限公司 一种核酸扩增阶段的荧光数据采集及判别方法
KR102407584B1 (ko) * 2020-08-21 2022-06-10 한림대학교 산학협력단 인공지능을 이용한 중합효소 연쇄반응 양성 판단을 위한 검사 방법
KR102407585B1 (ko) * 2020-08-21 2022-06-10 한림대학교 산학협력단 인공지능을 이용한 중합효소 연쇄반응 음성 판단을 위한 검사 방법
CN112816446B (zh) * 2020-12-24 2022-02-01 四川长虹电器股份有限公司 一种基于荧光光谱检测荧光轮粉体衰变的方法
CN112669910A (zh) * 2020-12-30 2021-04-16 杭州博日科技股份有限公司 扩增曲线基线确定方法、装置以及电子设备
WO2023027704A1 (en) * 2021-08-25 2023-03-02 Hewlett-Packard Development Company, L.P. Nucleic acid strand detections
AU2023386451A1 (en) 2022-11-22 2025-04-24 bioMérieux New nucleic acid binding compounds and uses
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4592365A (en) * 1981-08-10 1986-06-03 Ivac Corporation Electronic sphygmomanometer
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
EP1179600B1 (de) * 1996-06-04 2005-05-11 University Of Utah Research Foundation Überwachung der Hybridisierung während PCR
AU727296B2 (en) 1996-06-04 2000-12-07 University Of Utah Research Foundation System and methods for monitoring for DNA amplification by fluorescence
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification

Also Published As

Publication number Publication date
JP2000333700A (ja) 2000-12-05
US6387621B1 (en) 2002-05-14
DE60035459T2 (de) 2008-03-20
US20020042051A1 (en) 2002-04-11
EP1059523A2 (de) 2000-12-13
EP1059523B1 (de) 2007-07-11
ATE366821T1 (de) 2007-08-15
EP1059523A3 (de) 2003-05-21
US20020123062A1 (en) 2002-09-05
JP4689004B2 (ja) 2011-05-25

Similar Documents

Publication Publication Date Title
DE60035459D1 (de) Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation
ATE332398T1 (de) Einfach markierte oligonukleotidsonden
DE602006019947D1 (de) Verfahren zum nachweis von mehreren analyten
DE69535384D1 (de) Verfahen zum nachweis von nukleinsäure mit einer spezifischen sequenzzusammensetzung
DK0497784T5 (da) Kvantificering af nukleinsyrer under anvendelse af polymerasekædereaktion
WO2001084463A3 (en) Apparatus, method, and computer program product for quantitative analysis of a nucleic acid amplification reaction
ATE230801T1 (de) Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
CA2322858A1 (en) Detection of analytes by fluorescent lanthanide chelates
FI905701L (fi) Fluoresenssienergiansiirtoon perustuva biosensori ja menetelmä analyyttimolekyylien havaitsemiseksi sen avulla
DE60239968D1 (de) Sche
DE60136335D1 (de) Parallele genotypisierung mehrerer patientenproben
DE602004031152D1 (de) Verfahren und system zum analysieren von reaktione
DE60041489D1 (de) Nachweis von hiv-1 durch amplifizierung von nukleinsäuren
EA200500355A1 (ru) Способ обнаружения, характеристики и мониторинга углеводородных месторождений
DE60143355D1 (de) Verbessertes einfangen und detektion von zielnukleinsäuren in verfahren mit teststreifen
ATE272721T1 (de) Nukleinsäure hybridisierung-testverfahren in lösung
ATE330225T1 (de) Verfahren und testkit zum nachweis von analyten in einer probe
ATE197071T1 (de) Nachweis von nukleinsäure-amplifikation mittels fluoreszenz-polarisation
MX9604417A (es) Deteccion de analitos.
PT1173748E (pt) Metodo e dispositivo para detectar variacoes de propriedades opticas numa amostra a ser analisada
DE602004031571D1 (de) Enzymhemmungsimmunverfahren
DE60121547D1 (de) Electrochemisches verfahren zum nachweis von nukleinsäuren
ZA202207380B (en) Quantification of polynucleotide analytes from dried samples
ATE520034T1 (de) Quantitative analyse einer biologischen probe unbekannter menge
ATE305980T1 (de) Verwendung von abfangsonden beim nachweis von nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition