[go: up one dir, main page]

DE60012506D1 - Mischbett-festphase und dessen verwendung zur isolation von nukleinsäuren - Google Patents

Mischbett-festphase und dessen verwendung zur isolation von nukleinsäuren

Info

Publication number
DE60012506D1
DE60012506D1 DE60012506T DE60012506T DE60012506D1 DE 60012506 D1 DE60012506 D1 DE 60012506D1 DE 60012506 T DE60012506 T DE 60012506T DE 60012506 T DE60012506 T DE 60012506T DE 60012506 D1 DE60012506 D1 DE 60012506D1
Authority
DE
Germany
Prior art keywords
nucleic acids
nucleic acid
target nucleic
bed solid
solid phases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60012506T
Other languages
English (en)
Other versions
DE60012506T2 (de
Inventor
E Smith
L Holmes
J Simpson
Jehoshua Katzhendler
M Bitner
C Grosch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of DE60012506D1 publication Critical patent/DE60012506D1/de
Application granted granted Critical
Publication of DE60012506T2 publication Critical patent/DE60012506T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/02—Column or bed processes
    • B01J47/04—Mixed-bed processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60012506T 1999-05-14 2000-05-12 Mischbett-festphase und dessen verwendung zur isolation von nukleinsäuren Expired - Lifetime DE60012506T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US312139 1994-09-26
US09/312,139 US6270970B1 (en) 1999-05-14 1999-05-14 Mixed-bed solid phase and its use in the isolation of nucleic acids
PCT/US2000/012954 WO2000070041A1 (en) 1999-05-14 2000-05-12 Mixed-bed solid phase and its use in the isolation of nucleic acids

Publications (2)

Publication Number Publication Date
DE60012506D1 true DE60012506D1 (de) 2004-09-02
DE60012506T2 DE60012506T2 (de) 2005-07-28

Family

ID=23210049

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60012506T Expired - Lifetime DE60012506T2 (de) 1999-05-14 2000-05-12 Mischbett-festphase und dessen verwendung zur isolation von nukleinsäuren

Country Status (8)

Country Link
US (2) US6270970B1 (de)
EP (1) EP1179056B1 (de)
JP (1) JP2002543980A (de)
AT (1) ATE272111T1 (de)
AU (1) AU774810B2 (de)
CA (1) CA2372521C (de)
DE (1) DE60012506T2 (de)
WO (1) WO2000070041A1 (de)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
EP1036082B1 (de) * 1997-12-06 2002-05-29 DNA Research Innovations Limited Isolierung von nukleinsäuren
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
EP1147226B1 (de) * 1999-01-27 2013-01-23 Folim G. Halaka Materialien und verfahren zur reinigung von polyelektrolyten
DE10013990C2 (de) * 2000-03-22 2003-12-04 Invitek Biotechnik & Biodesign Polyfunktionelles Trägermaterial für die komplexe Nukleinsäureanalytik
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
EP1978110B1 (de) * 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-basiertes Verfahren und System zum Screenen von genomischer DNS
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
US20030207289A1 (en) * 2001-09-04 2003-11-06 Hodge Timothy A. Detection of genetic sequences using a bipartite probe
JP4198461B2 (ja) * 2000-11-13 2008-12-17 プロメガ コーポレイション シラン処理シリカ基質を用いた溶解物クリアランスおよび核酸単離
US20020106686A1 (en) * 2001-01-09 2002-08-08 Mckernan Kevin J. Methods and reagents for the isolation of nucleic acids
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7208271B2 (en) * 2001-11-28 2007-04-24 Applera Corporation Compositions and methods of selective nucleic acid isolation
US6833238B2 (en) 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
EP1468116A2 (de) 2002-01-16 2004-10-20 Dynal Biotech ASA Verfahren zur isolierung von nukleinsäuren und proteinen aus einer einzigen probe
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
GB0209382D0 (en) * 2002-04-24 2002-06-05 Genovision As Isolating nucleic acid
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040018559A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Size-exclusion ion-exchange particles
AU2003291931A1 (en) * 2002-11-08 2004-06-07 Invitek Gesellschaft Fur Biotechnik And Biodesign Mbh Novel buffer formulations for isolating, purifying and recovering long-chain and short-chain nucleic acids
JP2006520190A (ja) * 2003-01-21 2006-09-07 マイクロニクス， インコーポレイテッド 流体の微小流体的な操作、増幅、および分析（例えば、細菌アッセイおよびアンチグロブリン試験）のための方法およびシステム
EP1607748B1 (de) * 2003-03-24 2013-05-15 Sony Corporation Nukleinsäurenextraktions-Kit und Nukleinsäurenextraktionsverfahren
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
EP1508618B1 (de) * 2003-08-19 2012-07-25 Kurashiki Boseki Kabushiki Kaisha Extraktionsvorrichtung
US20050106576A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Methods of using cleavable solid phases for isolating nucleic acids
US20050106577A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Cleavable solid phases for isolating nucleic acids
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
SE0400490D0 (sv) * 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
WO2006015326A2 (en) * 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
CN101124321B (zh) 2004-11-05 2012-02-29 恰根北美控股有限公司 用于从稳定剂中纯化核酸的组合物和方法
EP1693108A1 (de) * 2004-12-04 2006-08-23 MERCK PATENT GmbH Mischmodus Anionenaustauschertrennungsmaterial
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060223073A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US20070015191A1 (en) * 2005-07-01 2007-01-18 Promega Corporation Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification
DE102005047736B4 (de) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
WO2007070381A2 (en) * 2005-12-09 2007-06-21 Promega Corporation Nucleic acid purification with a binding matrix
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
CA2668818C (en) 2006-10-10 2018-06-26 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
EP2099957A1 (de) * 2007-01-10 2009-09-16 GE Healthcare Bio-Sciences AB Multimodale ionenaustausch-chromotographie-harze
US8308940B2 (en) 2007-12-06 2012-11-13 The Board Of Trustees Of The University Of Illinois Chromatography devices and methods
JP5540467B2 (ja) * 2008-01-21 2014-07-02 Ｊｎｃ株式会社 上限臨界溶液温度を有する温度応答性ポリマーで表面修飾された磁性微粒子による電荷物質の回収方法及びキット
WO2010033627A2 (en) * 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
US20100305219A1 (en) * 2009-06-02 2010-12-02 The Board Of Trustees Of The University Of Illinois Emulsions and foams using patchy particles
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
EP2598223B1 (de) 2010-07-30 2022-04-06 EMD Millipore Corporation Chromatographiemedien und verfahren
DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
WO2012177656A2 (en) 2011-06-19 2012-12-27 Abogen, Inc. Devices, solutions and methods for sample collection
WO2013024072A1 (en) * 2011-08-12 2013-02-21 Qiagen Gmbh Method for isolating nucleic acids
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
US9315853B2 (en) 2012-01-30 2016-04-19 Exact Sciences Corporation Modification of DNA on magnetic beads
US11628381B2 (en) 2012-09-17 2023-04-18 W.R. Grace & Co. Conn. Chromatography media and devices
EP2983804A4 (de) * 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ionenaustauschreinigung von mrna
EP2925895B1 (de) 2013-03-15 2018-11-28 Avellino Lab USA, Inc. Verfahren zur verbesserten isolierung von genomischen dna-matrizen zum nachweis von allelen
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
EP2971161B1 (de) 2013-03-15 2018-12-26 ModernaTX, Inc. Nukleinsäurereinigung
EP3578663A1 (de) 2013-03-15 2019-12-11 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
ES2896755T3 (es) 2013-07-11 2022-02-25 Modernatx Inc Composiciones que comprenden polinucleótidos sintéticos que codifican proteínas relacionadas con CRISPR y ARNsg sintéticos y procedimientos de uso
AU2014305085A1 (en) * 2013-08-05 2016-02-25 The Johns Hopkins University Fabrication of hierarchical silica nanomembranes and uses thereof for solid phase extraction of nucleic acids
WO2015051169A2 (en) 2013-10-02 2015-04-09 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
TWI812047B (zh) 2013-11-15 2023-08-11 美商阿韋利諾美國實驗有限公司 用於多工偵測與眼科病況有關的對偶基因之方法
SG11201607364RA (en) * 2014-03-07 2016-10-28 Dna Genotek Inc Composition and method for stabilizing nucleic acids in biological samples
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
JP2017522028A (ja) 2014-07-16 2017-08-10 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． 環状ポリヌクレオチド
EP3230299A1 (de) 2014-12-08 2017-10-18 EMD Millipore Corporation Mischbettionenaustauschadsorber
US9422596B1 (en) * 2015-04-27 2016-08-23 Norgen Biotek Corp. Methods and columns for nucleic acid purification
JP2018517559A (ja) 2015-06-05 2018-07-05 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 吸着性バイオプロセス清澄化剤並びにその製造及び使用方法
PT3350333T (pt) 2015-09-17 2022-03-02 Modernatx Inc Polinucleótidos contendo uma região de cauda estabilizadora
US11434486B2 (en) 2015-09-17 2022-09-06 Modernatx, Inc. Polynucleotides containing a morpholino linker
ES2905558T3 (es) 2015-11-13 2022-04-11 Avellino Lab Usa Inc Procedimientos para el tratamiento de las distrofias corneales
WO2017193097A1 (en) 2016-05-06 2017-11-09 Girihlet Inc. Methods and compositions for determining specifc tcr and bcr chain pairings
CA3061620A1 (en) 2017-04-28 2018-11-01 Avellino Lab Usa, Inc. Methods for detecting alleles associated with keratoconus
WO2018215375A1 (en) * 2017-05-22 2018-11-29 General Electric Company Improvements in the recovery of nucleic acids from solid supports
CN112236519A (zh) 2018-03-03 2021-01-15 拉伯塞特股份有限公司 使用声学液滴喷射从样品中提取目标部分的系统和方法
LT3663401T (lt) 2019-02-28 2022-10-25 Lonza Ltd Biologinių molekulių, tokių kaip plazmidinė dnr, gryninimo būdas, panaudojant anijonų mainų chromatografiją

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4395271A (en) 1979-04-13 1983-07-26 Corning Glass Works Method for making porous magnetic glass and crystal-containing structures
US4297337A (en) 1979-04-13 1981-10-27 Corning Glass Works Solid-phase immunoassays using magnetic glass
US4233169A (en) 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US4298500A (en) 1980-05-05 1981-11-03 Varian Associates, Inc. Mixed phase chromatographic compositions
DE3211309A1 (de) 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
US4695393A (en) 1983-05-12 1987-09-22 Advanced Magnetics Inc. Magnetic particles for use in separations
US4672040A (en) 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4523996A (en) 1984-02-22 1985-06-18 Westinghouse Electric Corp. Method of separating cationic from anionic beads in mixed resin beds
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5076950A (en) 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
DE3639949A1 (de) 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4767670A (en) 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US5075430A (en) 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
EP0620761A1 (de) 1991-01-04 1994-10-26 Perseptive Biosystems, Inc. Durch sulfonamide gebundener hydrophiler überzug
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5395498A (en) 1991-11-06 1995-03-07 Gombinsky; Moshe Method for separating biological macromolecules and means therfor
US5610274A (en) 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US5734020A (en) 1991-11-20 1998-03-31 Cpg, Inc. Production and use of magnetic porous inorganic materials
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5316680A (en) 1992-10-21 1994-05-31 Cornell Research Foundation, Inc. Multimodal chromatographic separation media and process for using same
AT398973B (de) 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
AU6230594A (en) 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
DE4307262A1 (de) 1993-03-02 1994-09-08 Christian Bergemann Magnetisches polymeres Siliciumdioxid
ATE256735T1 (de) 1993-08-30 2004-01-15 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
DE59506355D1 (de) 1994-02-07 1999-08-12 Qiagen Gmbh Chromatographische isolierung von nucleinsäuren
US5990301A (en) 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
EP0743949B1 (de) 1994-02-07 1999-12-15 QIAGEN GmbH Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5654141A (en) 1994-11-18 1997-08-05 Thomas Jefferson University Amplification based detection of bacterial infection
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
AR003122A1 (es) 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
SE9600590D0 (sv) 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
JP3940935B2 (ja) 1996-06-11 2007-07-04 東洋紡績株式会社 プラスミドｄｎａの抽出精製方法
JPH09327291A (ja) 1996-06-11 1997-12-22 Toyobo Co Ltd Ｒｎａの抽出精製方法
ATE229371T1 (de) 1997-01-21 2002-12-15 Grace W R & Co Siliciumdioxidadsorbent auf magnetischem träger
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
EP1179056A1 (de) 2002-02-13
JP2002543980A (ja) 2002-12-24
WO2000070041A1 (en) 2000-11-23
US6376194B2 (en) 2002-04-23
DE60012506T2 (de) 2005-07-28
EP1179056B1 (de) 2004-07-28
AU4841500A (en) 2000-12-05
AU774810B2 (en) 2004-07-08
US6270970B1 (en) 2001-08-07
CA2372521A1 (en) 2000-11-23
CA2372521C (en) 2009-09-08
ATE272111T1 (de) 2004-08-15
US20020001812A1 (en) 2002-01-03

Similar Documents

Publication Publication Date Title
DE60012506D1 (de) Mischbett-festphase und dessen verwendung zur isolation von nukleinsäuren
PH12021550904A1 (en) Novel crispr/cas12f enzyme and system
GB2363795A (en) Process for nucleic acid purification using silicon carbide
ATE284891T1 (de) Isolierung von einzelsträngigen nukleinsäuren
EP1975172A3 (de) Hochaffine TGFß-Nukleinsäureliganden und -inhibitoren
PT1232502E (pt) Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
ES2122692T3 (es) Procedimiento de amplificacion de acidos nucleicos en fase solida y equipo de reactivos para su realizacion.
WO2003040687A3 (en) Isolation and purification of nucleic acids
BG107861A (en) Isothermal amplification of nucleic acids on a solid support
WO2005099770A3 (en) Pancreatic islet microrna and methods for inhibiting same
WO2000044928A3 (en) Materials and methods for the purification of polyelectrolytes
WO2007040592A8 (en) Making nucleic acid sequences in parallel and use
WO2001075087A3 (en) Subtilisin variants
CA2320100A1 (en) High affinity nucleic acid ligands of complement system proteins
DE60137652D1 (de) Magnetische isolierung und reinigung von nukleinsäuren
WO2005007895A1 (en) Methods and kits for obtaining nucleic acid from biological samples
WO2002040668A3 (de) Proteine und den proteinen zugrundeliegende dna-sequenzen mit funktion bei entzündungsereignissen
WO2020006131A3 (en) Nucleases for genome editing
MX2023014557A (es) Sistemas crispr-transposon para modificacion de adn.
WO2001064912A3 (en) Triazine degrading enzymes
WO2001066752A3 (en) Reproduction-specific genes
WO2001046686A3 (de) Chromatographiematerial und verfahren unter verwendung desselben
ATE311470T1 (de) Archivierung von vektoren
DE60130465D1 (de) Mutierte muskelspezifische enhancer
FR2792651B1 (fr) Sequence genomique et polypeptides de pyrococcus abyssi, leurs fragments et leurs utilisations

Legal Events

Date Code Title Description
8364 No opposition during term of opposition