DE50313016D1 - METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS - Google Patents
METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESSInfo
- Publication number
- DE50313016D1 DE50313016D1 DE50313016T DE50313016T DE50313016D1 DE 50313016 D1 DE50313016 D1 DE 50313016D1 DE 50313016 T DE50313016 T DE 50313016T DE 50313016 T DE50313016 T DE 50313016T DE 50313016 D1 DE50313016 D1 DE 50313016D1
- Authority
- DE
- Germany
- Prior art keywords
- classification
- implementing
- property determination
- analysis arrangement
- macrophage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 4
- 210000002540 macrophage Anatomy 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 abstract 2
- 102000011782 Keratins Human genes 0.000 abstract 1
- 108010076876 Keratins Proteins 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 210000000981 epithelium Anatomy 0.000 abstract 1
- 238000011156 evaluation Methods 0.000 abstract 1
- 238000000684 flow cytometry Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57434—Specifically defined cancers of prostate
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physiology (AREA)
- Ecology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
This invention relates to a method for determining and/or classifying the circulative macrophage and analyzing arrangement thereof. Firstly, separate themacrophage from the taken whole blood by means of acentrical motion in grads. Then , perforate the macrophage and apply a staining process in a cell with at least a selected antibody. Thirdly, perform a flow cytometry analysis of a pretreated cell to give a statistics evaluation of the followed cellular content. Usually, PSA, cytokeratin and/or epithelium antigen are chosen as antibody.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE50313016T DE50313016D1 (en) | 2002-06-26 | 2003-06-02 | METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10228548 | 2002-06-26 | ||
DE10230893A DE10230893A1 (en) | 2002-06-26 | 2002-07-09 | Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method |
PCT/EP2003/005763 WO2004003548A1 (en) | 2002-06-26 | 2003-06-02 | Method for the determination of characteristics and/or the classification of circulating macrophages, and analysis arrangement for carrying out said method |
DE50313016T DE50313016D1 (en) | 2002-06-26 | 2003-06-02 | METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS |
Publications (1)
Publication Number | Publication Date |
---|---|
DE50313016D1 true DE50313016D1 (en) | 2010-10-07 |
Family
ID=29723471
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE10230893A Withdrawn DE10230893A1 (en) | 2002-06-26 | 2002-07-09 | Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method |
DE50313016T Expired - Lifetime DE50313016D1 (en) | 2002-06-26 | 2003-06-02 | METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE10230893A Withdrawn DE10230893A1 (en) | 2002-06-26 | 2002-07-09 | Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method |
Country Status (4)
Country | Link |
---|---|
CN (1) | CN101813694A (en) |
AT (1) | ATE479093T1 (en) |
DE (2) | DE10230893A1 (en) |
ES (1) | ES2351481T3 (en) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6165460A (en) * | 1995-07-10 | 2000-12-26 | Therion Biologics Corporation | Generation of immune responses to prostate-specific antigen (PSA) |
DE19814915A1 (en) * | 1998-04-03 | 1999-10-07 | Roche Diagnostics Gmbh | Immunological method for the determination of PSA |
-
2002
- 2002-07-09 DE DE10230893A patent/DE10230893A1/en not_active Withdrawn
-
2003
- 2003-06-02 DE DE50313016T patent/DE50313016D1/en not_active Expired - Lifetime
- 2003-06-02 AT AT03735519T patent/ATE479093T1/en active
- 2003-06-02 CN CN200910208853A patent/CN101813694A/en active Pending
- 2003-06-02 ES ES03735519T patent/ES2351481T3/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE10230893A1 (en) | 2004-01-15 |
CN101813694A (en) | 2010-08-25 |
ATE479093T1 (en) | 2010-09-15 |
ES2351481T3 (en) | 2011-02-07 |
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