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DE50313016D1 - METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS - Google Patents

METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS

Info

Publication number
DE50313016D1
DE50313016D1 DE50313016T DE50313016T DE50313016D1 DE 50313016 D1 DE50313016 D1 DE 50313016D1 DE 50313016 T DE50313016 T DE 50313016T DE 50313016 T DE50313016 T DE 50313016T DE 50313016 D1 DE50313016 D1 DE 50313016D1
Authority
DE
Germany
Prior art keywords
classification
implementing
property determination
analysis arrangement
macrophage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE50313016T
Other languages
German (de)
Inventor
Ralf Herwig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE50313016T priority Critical patent/DE50313016D1/en
Priority claimed from PCT/EP2003/005763 external-priority patent/WO2004003548A1/en
Application granted granted Critical
Publication of DE50313016D1 publication Critical patent/DE50313016D1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407Specifically defined cancers
    • G01N33/57434Specifically defined cancers of prostate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physiology (AREA)
  • Ecology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to a method for determining and/or classifying the circulative macrophage and analyzing arrangement thereof. Firstly, separate themacrophage from the taken whole blood by means of acentrical motion in grads. Then , perforate the macrophage and apply a staining process in a cell with at least a selected antibody. Thirdly, perform a flow cytometry analysis of a pretreated cell to give a statistics evaluation of the followed cellular content. Usually, PSA, cytokeratin and/or epithelium antigen are chosen as antibody.
DE50313016T 2002-06-26 2003-06-02 METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS Expired - Lifetime DE50313016D1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE50313016T DE50313016D1 (en) 2002-06-26 2003-06-02 METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10228548 2002-06-26
DE10230893A DE10230893A1 (en) 2002-06-26 2002-07-09 Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method
PCT/EP2003/005763 WO2004003548A1 (en) 2002-06-26 2003-06-02 Method for the determination of characteristics and/or the classification of circulating macrophages, and analysis arrangement for carrying out said method
DE50313016T DE50313016D1 (en) 2002-06-26 2003-06-02 METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS

Publications (1)

Publication Number Publication Date
DE50313016D1 true DE50313016D1 (en) 2010-10-07

Family

ID=29723471

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10230893A Withdrawn DE10230893A1 (en) 2002-06-26 2002-07-09 Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method
DE50313016T Expired - Lifetime DE50313016D1 (en) 2002-06-26 2003-06-02 METHOD FOR THE PROPERTY DETERMINATION AND / OR CLASSIFICATION OF CIRCULATING MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE PROCESS

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10230893A Withdrawn DE10230893A1 (en) 2002-06-26 2002-07-09 Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method

Country Status (4)

Country Link
CN (1) CN101813694A (en)
AT (1) ATE479093T1 (en)
DE (2) DE10230893A1 (en)
ES (1) ES2351481T3 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6165460A (en) * 1995-07-10 2000-12-26 Therion Biologics Corporation Generation of immune responses to prostate-specific antigen (PSA)
DE19814915A1 (en) * 1998-04-03 1999-10-07 Roche Diagnostics Gmbh Immunological method for the determination of PSA

Also Published As

Publication number Publication date
DE10230893A1 (en) 2004-01-15
CN101813694A (en) 2010-08-25
ATE479093T1 (en) 2010-09-15
ES2351481T3 (en) 2011-02-07

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