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ATE479093T1 - METHOD FOR DETERMINING PROPERTIES AND/OR CLASSIFICATION OF CIRCULATIVE MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE METHOD - Google Patents

METHOD FOR DETERMINING PROPERTIES AND/OR CLASSIFICATION OF CIRCULATIVE MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE METHOD

Info

Publication number
ATE479093T1
ATE479093T1 AT03735519T AT03735519T ATE479093T1 AT E479093 T1 ATE479093 T1 AT E479093T1 AT 03735519 T AT03735519 T AT 03735519T AT 03735519 T AT03735519 T AT 03735519T AT E479093 T1 ATE479093 T1 AT E479093T1
Authority
AT
Austria
Prior art keywords
circulative
macrophages
classification
implementing
determining properties
Prior art date
Application number
AT03735519T
Other languages
German (de)
Inventor
Ralf Herwig
Original Assignee
Ralf Herwig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ralf Herwig filed Critical Ralf Herwig
Priority claimed from PCT/EP2003/005763 external-priority patent/WO2004003548A1/en
Application granted granted Critical
Publication of ATE479093T1 publication Critical patent/ATE479093T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57434—Specifically defined cancers of prostate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physiology (AREA)
  • Ecology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to a method for determining and/or classifying the circulative macrophage and analyzing arrangement thereof. Firstly, separate themacrophage from the taken whole blood by means of acentrical motion in grads. Then , perforate the macrophage and apply a staining process in a cell with at least a selected antibody. Thirdly, perform a flow cytometry analysis of a pretreated cell to give a statistics evaluation of the followed cellular content. Usually, PSA, cytokeratin and/or epithelium antigen are chosen as antibody.
AT03735519T 2002-06-26 2003-06-02 METHOD FOR DETERMINING PROPERTIES AND/OR CLASSIFICATION OF CIRCULATIVE MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE METHOD ATE479093T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10228548 2002-06-26
DE10230893A DE10230893A1 (en) 2002-06-26 2002-07-09 Method for determining the properties and / or classification of circulating macrophages and / or peripheral blood mononuclear cells and analysis arrangement for carrying out the method
PCT/EP2003/005763 WO2004003548A1 (en) 2002-06-26 2003-06-02 Method for the determination of characteristics and/or the classification of circulating macrophages, and analysis arrangement for carrying out said method

Publications (1)

Publication Number Publication Date
ATE479093T1 true ATE479093T1 (en) 2010-09-15

Family

ID=29723471

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03735519T ATE479093T1 (en) 2002-06-26 2003-06-02 METHOD FOR DETERMINING PROPERTIES AND/OR CLASSIFICATION OF CIRCULATIVE MACROPHAGES AND ANALYSIS ARRANGEMENT FOR IMPLEMENTING THE METHOD

Country Status (4)

Country Link
CN (1) CN101813694A (en)
AT (1) ATE479093T1 (en)
DE (2) DE10230893A1 (en)
ES (1) ES2351481T3 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6165460A (en) * 1995-07-10 2000-12-26 Therion Biologics Corporation Generation of immune responses to prostate-specific antigen (PSA)
DE19814915A1 (en) * 1998-04-03 1999-10-07 Roche Diagnostics Gmbh Immunological method for the determination of PSA

Also Published As

Publication number Publication date
ES2351481T3 (en) 2011-02-07
CN101813694A (en) 2010-08-25
DE10230893A1 (en) 2004-01-15
DE50313016D1 (en) 2010-10-07

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