[go: up one dir, main page]

DE3588008D1 - Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung. - Google Patents

Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung.

Info

Publication number
DE3588008D1
DE3588008D1 DE3588008T DE3588008T DE3588008D1 DE 3588008 D1 DE3588008 D1 DE 3588008D1 DE 3588008 T DE3588008 T DE 3588008T DE 3588008 T DE3588008 T DE 3588008T DE 3588008 D1 DE3588008 D1 DE 3588008D1
Authority
DE
Germany
Prior art keywords
yeast
production
cellulolytic enzymes
yeast strains
cellulolytic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3588008T
Other languages
English (en)
Other versions
DE3588008T2 (de
Inventor
Jonathan Knowles
Merja Penttilae
Tuula Teeri
Helena Nevalainen
Irma Salovuori
Paeivi Lehtovaara-Helenius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AB Enzymes Oy
Original Assignee
Alko Oy AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8518918&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3588008(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Alko Oy AB filed Critical Alko Oy AB
Publication of DE3588008D1 publication Critical patent/DE3588008D1/de
Application granted granted Critical
Publication of DE3588008T2 publication Critical patent/DE3588008T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • C12N15/625DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/911Microorganisms using fungi
    • Y10S435/945Trichoderma

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE3588008T 1984-04-13 1985-04-12 Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung. Expired - Fee Related DE3588008T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI841500A FI841500A0 (fi) 1984-04-13 1984-04-13 Foerfarande foer uppbygnande av cellulolytiska jaeststammar.
PCT/FI1985/000039 WO1985004672A1 (en) 1984-04-13 1985-04-12 Yeast strains producing cellulolytic enzymes and methods and means for constructing them

Publications (2)

Publication Number Publication Date
DE3588008D1 true DE3588008D1 (de) 1995-05-18
DE3588008T2 DE3588008T2 (de) 1995-10-12

Family

ID=8518918

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3588008T Expired - Fee Related DE3588008T2 (de) 1984-04-13 1985-04-12 Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung.

Country Status (8)

Country Link
US (3) US4894338A (de)
EP (2) EP0312121A3 (de)
AT (1) ATE121132T1 (de)
CA (1) CA1305931C (de)
DE (1) DE3588008T2 (de)
DK (1) DK580385D0 (de)
FI (2) FI841500A0 (de)
WO (1) WO1985004672A1 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5472864A (en) * 1984-04-19 1995-12-05 Genencor International, Inc. Method of preparing solution enriched in EG III using low molecular weight alcohol, organic salt and inorganic salt
US5320960A (en) * 1992-04-03 1994-06-14 Genencor International, Inc. Method of preparing solution enriched in xylanase using low molecular weight alcohol, organic salt and inorganic salt
US5298405A (en) * 1986-04-30 1994-03-29 Alko Limited Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
US5610034A (en) * 1987-04-29 1997-03-11 Alko Group Ltd. Immunoglobulin production by trichoderma
EP0406314B1 (de) * 1988-03-24 1993-12-01 Novo Nordisk A/S Cellulosezubereitung
US5648263A (en) * 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
US5688290A (en) * 1989-10-19 1997-11-18 Genencor International, Inc. Degradation resistant detergent compositions based on cellulase enzymes
US5866382A (en) 1990-04-06 1999-02-02 Xyrofin Oy Xylose utilization by recombinant yeasts
US5168064A (en) * 1990-04-20 1992-12-01 The Regents Of The University Of California Endo-1,4-β-glucanase gene and its use in plants
NL9001388A (nl) * 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
WO1992006209A1 (en) * 1990-10-05 1992-04-16 Genencor International Inc. Trichoderma reesei containing deleted and/or enriched cellulase and other enzyme genes and cellulase compositions derived therefrom
US5654193A (en) * 1990-10-05 1997-08-05 Genencor International, Inc. Methods for treating cotton containing fabrics with cellulase
US5525507A (en) * 1990-10-05 1996-06-11 Genencor International, Inc. Methods for treating cotton-containing fabric with cellulase composition containing endoglucanase component and which is free of all CBH I component
US5246853A (en) * 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
WO1992006183A1 (en) * 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
US5650322A (en) * 1990-10-05 1997-07-22 Genencor International, Inc. Methods for stonewashing fabrics using endoglucanases
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
US5475101A (en) * 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
WO1992006221A1 (en) * 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
US5443750A (en) * 1991-01-16 1995-08-22 The Procter & Gamble Company Detergent compositions with high activity cellulase and softening clays
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
CA2107208A1 (en) 1991-03-29 1992-09-30 Kathleen A. Clarkson Methods for treating cotton-containing fabrics with cellulase
US6512166B1 (en) 1991-06-17 2003-01-28 Cornell Research Foundation, Inc. Combinations of fungal cell wall degrading enzyme and fungal cell membrane affecting compound
CA2134446A1 (en) * 1992-05-01 1993-11-11 Kathleen A. Clarkson Methods for treating cotton-containing fabrics with cbh i enriched cellulase
FI92500C (fi) * 1993-03-03 1994-11-25 Valtion Teknillinen Menetelmä mekaanisen massan valmistamiseksi
US7005128B1 (en) 1993-12-17 2006-02-28 Genencor International, Inc. Enzyme feed additive and animal feed including it
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US6268196B1 (en) 1993-12-17 2001-07-31 Genencor International, Inc. Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions
US6117664A (en) * 1994-03-03 2000-09-12 Novo Nordisk A/S Alkaline cellulases
CA2185101A1 (en) * 1994-03-08 1995-09-14 Martin Schulein Novel alkaline cellulases
US7816129B2 (en) 1994-07-29 2010-10-19 Ab Enzymes Gmbh Production and secretion of proteins of bacterial origin in filamentous fungi
US6723549B2 (en) * 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
TR199800934T2 (xx) * 1995-11-27 1999-02-22 Unilever N.V. Enzimatik deterjan bile�imleri.
WO1997033982A1 (fr) * 1996-03-14 1997-09-18 JAPAN, represented by DIRECTOR GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY Proteine ayant une activite de cellulase et son procede de production
US7465571B1 (en) 1996-05-22 2008-12-16 Verenium Corporation Endoglucanases
US5789228A (en) * 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
ES2242234T3 (es) * 1996-09-12 2005-11-01 Syngenta Participations Ag Plantas transgenicas que expresan enzimas celuloliticas.
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
FI964692A0 (fi) * 1996-11-25 1996-11-25 Primalco Ltd Foerbaettrad cellulassammansaettning foer bioefterbehandling av textilmaterial som innehaoller cellulosa
FI964691A0 (fi) * 1996-11-25 1996-11-25 Primalco Ltd Foerbaettrad cellulassammasaettning foer behandling av textilmaterial som innehaoller cellulosa
US6001595A (en) * 1996-11-29 1999-12-14 Rohm Enzyme GmbH Promoters and uses thereof
US6818803B1 (en) 1997-06-26 2004-11-16 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
DE69827712T3 (de) * 1997-07-31 2009-07-09 Dsm Ip Assets B.V. Zusammensetzung für Brotverbesserung
NZ502444A (en) * 1997-07-31 2001-11-30 Dsm N Polypeptides with cellobiohydrolase activity (CBH A and CBH B) from Aspergillus
US6222028B1 (en) 1999-10-15 2001-04-24 Academia Sinica Polynucleotides encoding cellulase enzymes from Piromyces rhizinflata
US6428996B1 (en) 1999-10-27 2002-08-06 Academia Sinica Cellulase enzymes
ATE327322T1 (de) * 2000-09-25 2006-06-15 Iogen Energy Corp Verfahren zur herstellung von glucose mit ein ermodifizierten zellulase
AR036370A1 (es) 2001-08-27 2004-09-01 Syngenta Participations Ag Plantas con auto-procesamiento y partes de las mismas
EP1485495B1 (de) * 2002-03-15 2010-09-15 Iogen Energy Corporation Verfahren zur herstellung von glukose durch verwendung von endoglucanase-core-protein zur verbesserten rückgewinnung und wiederverwendung des enzyms
AU2003291962A1 (en) 2002-12-20 2004-07-14 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
CA2558603A1 (en) * 2004-03-08 2005-10-20 Syngenta Participations Ag Self-processing plants and plant parts
DE602006010051D1 (de) * 2005-04-27 2009-12-10 Novozymes Inc Polypeptide mit endoglucanase-aktivtät und dafür codierende polynukleotide
CN101652381B (zh) * 2007-01-30 2014-04-09 维莱尼姆公司 用于处理木质纤维素的酶、编码它们的核酸及其制备和应用方法
US9068176B2 (en) 2011-01-26 2015-06-30 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2013016207A2 (en) * 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
US8778639B1 (en) * 2013-02-12 2014-07-15 Novozymes Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US8771993B1 (en) * 2013-02-12 2014-07-08 Novozymes A/S Polypeptides having endoglucanse activity and polynucleotides encoding same
FR3022558B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
CN113322190B (zh) * 2021-06-24 2023-07-18 西北农林科技大学 里氏木霉与酿酒酵母混合发酵白酒糟生产单细胞蛋白的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4165974A (en) * 1973-03-16 1979-08-28 Goodwin George I Mold lubricant and method
US4275163A (en) * 1978-11-20 1981-06-23 The United States Of America As Represented By The Secretary Of The Army Cellulase-producing microorganism
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
IE54046B1 (en) * 1981-08-25 1989-05-24 Celltech Ltd Expression vectors
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
FR2529569B1 (fr) * 1982-07-02 1985-07-05 Pasteur Institut Microorganismes, notamment e. coli, transformes par une sequence d'adn originaire de c. thermocellum, compositions a activite cellulolytique issues de ces micro-organismes et procede pour les obtenir
CA1338400C (en) * 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US4794175A (en) * 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
EP0191058B1 (de) * 1984-08-01 1989-03-29 Cl Pharma Aktiengesellschaft Immunwirksame verbindungen

Also Published As

Publication number Publication date
ATE121132T1 (de) 1995-04-15
FI86745C (fi) 1992-10-12
DK580385A (da) 1985-12-13
CA1305931C (en) 1992-08-04
FI864110A (fi) 1986-10-10
EP0214971A1 (de) 1987-03-25
US4894338A (en) 1990-01-16
DK580385D0 (da) 1985-12-13
EP0312121A2 (de) 1989-04-19
FI864110A0 (fi) 1986-10-10
EP0312121A3 (de) 1989-12-13
EP0214971B1 (de) 1995-04-12
FI841500A0 (fi) 1984-04-13
FI86745B (fi) 1992-06-30
DE3588008T2 (de) 1995-10-12
US5529919A (en) 1996-06-25
WO1985004672A1 (en) 1985-10-24
US5393670A (en) 1995-02-28

Similar Documents

Publication Publication Date Title
DE3588008D1 (de) Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung.
DK0778348T3 (da) Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr
KR850001528A (ko) 열안정성 글루코아밀라제의 제법
ATE77104T1 (de) Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute.
EP1095158A4 (de) Verfahren und materialien zur herstellung von glucosamin
MX165920B (es) Un procedimiento para producir glucoamilasa
DE69723855D1 (de) Verfahren zur herstellung von polypeptiden in surfaktinmutanten von bacillus zellen
ATE114721T1 (de) Immobilisierte hefe verwendende methode zur herstellung von ethanol und alkoholischen getränken.
Hayashida et al. Production and purification of thermostable cellulases from Humicola insolens YH-8
Amirul et al. Purification and properties of two forms of glucoamylase from Aspergillus niger
DE68925097D1 (de) Biokatalysatoren
ATE31077T1 (de) Thermostabile, saure alpha-amylase und verfahren zu ihrer herstellung.
ATE123304T1 (de) Durch rekombinante dns-technologie konstruierte amylolytische enzymproduzierende mikroorganismen und ihre verwendung in gärungsverfahren.
SAWAI Studies on an Amylase of Candida tropicalis Var. Japonica I. maltase and Transglucosylase Activities of the Amylase
DE3582020D1 (de) Thermostabile alpha-amylase produzierende thermostabile anaerobe bakterie, thermostabile alpha-amylase und verfahren zur deren herstellung.
KR890002235A (ko) 말토테트라오즈 생성 아밀라아제 활성을 지닌 폴리페프티드와 그 용도
JPH10501694A (ja) ピロジクチウム・キシラナーゼ、アミラーゼ及びプルラナーゼ
EP2281892A3 (de) Verfahren zur Herstellung von Polypeptiden in atmungsdefizienten Trichoderma Zellen
ATE46363T1 (de) Klonierungs- und expressionsplasmidvektoren fuer ein protein in einem mikroorganismus die mindestens den expressionspromotor von betaglucosidase in den hefen enthalten, dieselben enthaltende mikroorganismen, gaerungsverfahren und erhaltene enzyme.
Lastick et al. Simultaneous saccharification and fermentation of cellulose.
DE3850456T2 (de) Klonierung des isoamylaseenzym kodierenden Gens und seine Verwendung zur Herstellung dieses Enzyms.
McHale et al. Cellulase production during growth of Talaromyces emersonii CBS 814.70 on lactose containing media
BR9404998A (pt) DNA isolado e purificado, polinucleotídeo recombinante compreendendo o DNA, célula hospedeira, substância proteinácea, processo para produzir uma substância proteinácea e para hidrolisar dextrano
JPH0121957B2 (de)
DE60014655T2 (de) Polypeptide

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ROEHM ENZYME FINLAND OY, HELSINKI, FI

8339 Ceased/non-payment of the annual fee