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DE3486216D1 - Hybrid-dns-synthesis von reifen insulinaehnlichen wachstumsfaktoren. - Google Patents

Hybrid-dns-synthesis von reifen insulinaehnlichen wachstumsfaktoren.

Info

Publication number
DE3486216D1
DE3486216D1 DE84104175T DE3486216T DE3486216D1 DE 3486216 D1 DE3486216 D1 DE 3486216D1 DE 84104175 T DE84104175 T DE 84104175T DE 3486216 T DE3486216 T DE 3486216T DE 3486216 D1 DE3486216 D1 DE 3486216D1
Authority
DE
Germany
Prior art keywords
igf
host
gene
yeast
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE84104175T
Other languages
English (en)
Other versions
DE3486216T2 (de
Inventor
Philip J Barr
James P Merryweather
Guy Mullenbach
Mickey S Urdea
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of DE3486216D1 publication Critical patent/DE3486216D1/de
Publication of DE3486216T2 publication Critical patent/DE3486216T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE84104175T 1983-04-25 1984-04-13 Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren. Expired - Fee Related DE3486216T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48795083A 1983-04-25 1983-04-25

Publications (2)

Publication Number Publication Date
DE3486216D1 true DE3486216D1 (de) 1993-11-04
DE3486216T2 DE3486216T2 (de) 1994-02-17

Family

ID=23937779

Family Applications (2)

Application Number Title Priority Date Filing Date
DE84104175T Expired - Fee Related DE3486216T2 (de) 1983-04-25 1984-04-13 Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
DE3486491T Expired - Lifetime DE3486491T2 (de) 1983-04-25 1984-04-13 Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE3486491T Expired - Lifetime DE3486491T2 (de) 1983-04-25 1984-04-13 Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren

Country Status (17)

Country Link
US (1) US6642029B1 (de)
EP (2) EP0561137B1 (de)
JP (2) JP2548105B2 (de)
AT (2) ATE95236T1 (de)
AU (1) AU576757B2 (de)
DE (2) DE3486216T2 (de)
DK (1) DK204584A (de)
ES (1) ES531855A0 (de)
FI (1) FI87799C (de)
HK (1) HK65594A (de)
HU (1) HU204565B (de)
IE (2) IE60228B1 (de)
IL (1) IL71634A (de)
NO (1) NO174302C (de)
NZ (1) NZ207842A (de)
PT (1) PT78484B (de)
ZA (1) ZA842982B (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
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NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US7198919B1 (en) 1983-04-25 2007-04-03 Genentech, Inc. Use of alpha factor sequences in yeast expression systems
US4588684A (en) * 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
JPS60501989A (ja) * 1983-08-10 1985-11-21 アムジエン インシュリン様成長因子の微生物発現
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
DK108685A (da) * 1984-03-19 1985-09-20 Fujisawa Pharmaceutical Co Vaekstfaktor i
US4738921A (en) * 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
JP2549504B2 (ja) * 1984-12-21 1996-10-30 ア−ス製薬株式会社 Ｄｎａ塩基配列、ポリペプチド分泌発現ベクター及び形質転換微生物
GB8507833D0 (en) * 1985-03-26 1985-05-01 Biogen Nv Production of human somatomedin c
US5242811A (en) * 1985-03-26 1993-09-07 Biogen, Inc. Production of human somatomedin C
US5342921A (en) * 1985-03-28 1994-08-30 Chiron Corporation Superoxide dismutase fusion polypeptides for expression of mammalian proteins
ATE63757T1 (de) * 1985-03-28 1991-06-15 Chiron Corp Expression durch verwendung von fusionsgenen fuer proteinproduktion.
US4751180A (en) * 1985-03-28 1988-06-14 Chiron Corporation Expression using fused genes providing for protein product
US4783524A (en) * 1985-09-17 1988-11-08 Monsanto Company Growth factors
ATE67243T1 (de) * 1985-10-21 1991-09-15 Fujisawa Pharmaceutical Co Verfahren zur herstellung von insulinaehnlichem wachstumsfaktor i (igf-i) und plasmid zu dessen herstellung.
GB8529014D0 (en) * 1985-11-25 1986-01-02 Biogen Nv Enhanced secretion of heterologous proteins
EP0234862A3 (de) * 1986-02-28 1988-10-05 Merck & Co. Inc. Expression rekombinanter Proteine in Hefe
EP0234871A3 (de) * 1986-02-28 1988-10-12 Merck & Co. Inc. Regulierbare Expression rekombinanter Proteine in Hefe
JPH0616716B2 (ja) * 1986-10-14 1994-03-09 塩野義製薬株式会社 酵母におけるヒトｐｓｔｉの製造法
DE3726655A1 (de) * 1987-08-11 1989-02-23 Hoechst Ag Verfahren zur isolierung basischer proteine aus proteingemischen, welche solche basischen proteine enthalten
GB8723660D0 (en) * 1987-10-08 1987-11-11 British Bio Technology Synthetic gene
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US4988675A (en) * 1988-02-05 1991-01-29 Ciba-Geigy Corporation Method for preventing secondary effects
DE68905203T2 (de) * 1988-02-05 1993-07-22 Ciba Geigy Ag Verwendung von igf i zur herstellung eines praeparates fuer die behandlung von nierenkrankheiten.
GB8819826D0 (en) * 1988-08-20 1988-09-21 Kabivitrum Ab Glycosylated igf-1
GB8927008D0 (en) * 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli
NZ236618A (en) * 1990-01-03 1997-06-24 Ciba Geigy Ag Treating and preventing osteoporosis using insulin-like growth factor i (igf i) in conjunction with a bone antiresorptive active compound
US5374620A (en) * 1990-06-07 1994-12-20 Genentech, Inc. Growth-promoting composition and its use
US5681814A (en) * 1990-06-07 1997-10-28 Genentech, Inc. Formulated IGF-I Composition
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
TW267102B (de) 1992-03-13 1996-01-01 Ciba Geigy
SE9201573D0 (sv) * 1992-05-19 1992-05-19 Kabi Pharmacia Ab Use of igf-1
US5521086A (en) * 1993-09-16 1996-05-28 Cephalon, Inc. Secretion sequence for the production of a heterologous protein in yeast
SE9402370D0 (sv) 1994-07-04 1994-07-04 Pharmacia Ab Use of IGF-I
EP0779927A1 (de) * 1994-09-08 1997-06-25 Chiron Corporation Methode zur gesteigerten herstellung von insulin-ähnlichem wachstumsfaktor
US5712249A (en) * 1994-09-08 1998-01-27 Ciba-Geigy Corporation Use of insulin-like growth factors I and II for inhibition of inflammatory response
EP0871730B1 (de) 1995-10-11 2004-08-18 Chiron Corporation Kombination pdgf, kgf, igf und igfbp zur wundheilung
US5783556A (en) * 1996-08-13 1998-07-21 Genentech, Inc. Formulated insulin-containing composition
US6602705B1 (en) 1998-12-31 2003-08-05 Chiron Corporation Expression of HIV polypeptides and production of virus-like particles
PT1165119E (pt) 1999-04-08 2004-02-27 Genentech Inc Composicao baseada em polipeptidos com cargas opostas
WO2003093417A2 (en) 2002-05-01 2003-11-13 Cell Genesys, Inc. Lentiviral vector particles resistant to complement inactivation
CN102861321A (zh) 2003-09-12 2013-01-09 特西卡股份有限公司 胰岛素样生长因子-1(igf-1)缺陷的治疗方法
EP1674113A1 (de) 2004-12-22 2006-06-28 F. Hoffmann-La Roche Ag Konjugate von insulinähnlichem Wachstumfaktor-1 und Poly(Ethylenglykol)
KR101106795B1 (ko) 2006-08-31 2012-01-18 에프. 호프만-라 로슈 아게 인슐린-유사 성장 인자-ｉ의 제조 방법
CL2007002502A1 (es) 2006-08-31 2008-05-30 Hoffmann La Roche Variantes del factor de crecimiento similar a insulina-1 humano (igf-1) pegilados en lisina; metodo de produccion; proteina de fusion que la comprende; y su uso para tratar la enfermedad de alzheimer.
PE20090368A1 (es) 2007-06-19 2009-04-28 Boehringer Ingelheim Int Anticuerpos anti-igf
UY32317A (es) 2008-12-12 2010-07-30 Boehringer Ingelheim Int Anticuerpos anti-igf
BR112012000865A2 (pt) 2009-07-17 2019-09-24 T Tabor Aaron "composição e método para a modificação genética cosmética de células substancialmente intactas"
US20110152188A1 (en) 2009-12-23 2011-06-23 Hanns-Christian Mahler Pharmaceutical compositions of igf/i proteins
US20140255413A1 (en) 2013-03-07 2014-09-11 Boehringer Ingelheim International Gmbh Combination therapy for neoplasia treatment
WO2021005604A1 (en) 2019-07-11 2021-01-14 Opko Biologics Ltd. Long-acting igf-1 or igf-1 variants and methods of producing same

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
US4443539A (en) 1980-02-05 1984-04-17 The Upjohn Company Process for preparing bovine growth hormone
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
US4546082A (en) 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
DE3382547D1 (de) * 1983-01-12 1992-05-27 Chiron Corp Sekretorische expression in eukaryoten.
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
GB8308483D0 (en) * 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
JPS60501140A (ja) * 1983-04-22 1985-07-25 アムジエン 酵母による外因性ポリペプチドの分泌
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
AU2722184A (en) * 1983-04-25 1984-11-01 Genentech Inc. Use of alpha sequences in yeast expression systems
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
US4745179A (en) * 1984-04-02 1988-05-17 Fujisawa Pharmaceutical Co., Ltd. 59 Valine insulin-like growth factor I and process for production thereof
CA1341423C (en) * 1984-10-31 2003-03-04 Paul A. Luciw Recombinant proteins of viruses associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome
EP0193112A3 (de) 1985-02-22 1987-02-25 The Trustees Of Columbia University In The City Of New York Für einen insulinähnlichen Säugetier-Wachstumsfaktor II kodierende cDNS
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
GB8927008D0 (en) * 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5521086A (en) * 1993-09-16 1996-05-28 Cephalon, Inc. Secretion sequence for the production of a heterologous protein in yeast
ATE253120T1 (de) 1996-12-13 2003-11-15 Chiron Corp Verfahren zur expression von heterologen proteinen in hefe

Also Published As

Publication number Publication date
JPH07143886A (ja) 1995-06-06
FI87799B (fi) 1992-11-13
AU2723384A (en) 1984-11-01
DK204584A (da) 1984-10-26
IE940140L (en) 1984-10-25
JP2530424B2 (ja) 1996-09-04
JP2548105B2 (ja) 1996-10-30
IE60228B1 (en) 1994-06-15
FI87799C (fi) 1993-02-25
ATE220101T1 (de) 2002-07-15
HK65594A (en) 1994-07-15
ES8505412A1 (es) 1985-05-16
JPS59205997A (ja) 1984-11-21
AU576757B2 (en) 1988-09-08
ATE95236T1 (de) 1993-10-15
FI841524A0 (fi) 1984-04-16
NO174302C (no) 1994-04-13
NO174302B (no) 1994-01-03
US6642029B1 (en) 2003-11-04
PT78484B (fr) 1986-05-22
DK204584D0 (da) 1984-04-24
IL71634A (en) 1992-09-06
EP0123228B1 (de) 1993-09-29
EP0123228A3 (en) 1987-03-25
IL71634A0 (en) 1984-07-31
DE3486216T2 (de) 1994-02-17
EP0123228A2 (de) 1984-10-31
FI841524L (fi) 1984-10-26
PT78484A (fr) 1984-05-01
ES531855A0 (es) 1985-05-16
IE840975L (en) 1984-10-25
DE3486491D1 (de) 2002-08-08
DE3486491T2 (de) 2003-01-16
HU204565B (en) 1992-01-28
ZA842982B (en) 1984-11-28
HUT34236A (en) 1985-02-28
EP0561137B1 (de) 2002-07-03
EP0561137A1 (de) 1993-09-22
NZ207842A (en) 1988-02-12
NO841613L (no) 1984-10-26

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee