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DE3161331D1 - Process for determining hl antigens - Google Patents

Process for determining hl antigens

Info

Publication number
DE3161331D1
DE3161331D1 DE8181108755T DE3161331T DE3161331D1 DE 3161331 D1 DE3161331 D1 DE 3161331D1 DE 8181108755 T DE8181108755 T DE 8181108755T DE 3161331 T DE3161331 T DE 3161331T DE 3161331 D1 DE3161331 D1 DE 3161331D1
Authority
DE
Germany
Prior art keywords
complement
atp
antigens
determining
lymphocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8181108755T
Other languages
German (de)
Inventor
Reiner Dr Scherer
Karl Dr Wulff
Christine Treffert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE8181108755T priority Critical patent/DE3161331D1/en
Application granted granted Critical
Publication of DE3161331D1 publication Critical patent/DE3161331D1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1. Process for the determination of HL antigens on lymphocytes by incubation of the lymphocytes with HLA antiserum and complement, with the liberation of ATP from the cells lysed by complement, characterised in that one reacts the liberated ATP with an ATPase, subsequently rapidly lyses the cells not lysed by complement and determines the ATP liberated therefrom.
DE8181108755T 1980-12-18 1981-10-22 Process for determining hl antigens Expired DE3161331D1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE8181108755T DE3161331D1 (en) 1980-12-18 1981-10-22 Process for determining hl antigens

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19803047860 DE3047860A1 (en) 1980-12-18 1980-12-18 METHOD FOR DETERMINING HLA ANTIGENS
DE8181108755T DE3161331D1 (en) 1980-12-18 1981-10-22 Process for determining hl antigens

Publications (1)

Publication Number Publication Date
DE3161331D1 true DE3161331D1 (en) 1983-12-08

Family

ID=6119575

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19803047860 Withdrawn DE3047860A1 (en) 1980-12-18 1980-12-18 METHOD FOR DETERMINING HLA ANTIGENS
DE8181108755T Expired DE3161331D1 (en) 1980-12-18 1981-10-22 Process for determining hl antigens

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE19803047860 Withdrawn DE3047860A1 (en) 1980-12-18 1980-12-18 METHOD FOR DETERMINING HLA ANTIGENS

Country Status (4)

Country Link
EP (1) EP0054676B1 (en)
JP (1) JPS57131063A (en)
AT (1) ATE5201T1 (en)
DE (2) DE3047860A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3382137D1 (en) * 1982-07-30 1991-02-28 Bernard Francois Mach DNA SEQUENCE ENCODING FOR THE DR BETA CHAIN LOCUS OF THE HUMAN LYMPHOCYTANTIGEN COMPLEX AND POLYPEPTIDES, DIAGNOSTIC TYPING METHODS AND PRODUCTS RELATING TO THIS.
US6818393B1 (en) 1982-07-30 2004-11-16 Biomirieux Sa DNA sequences coding for the DR beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto
GB8610426D0 (en) * 1986-04-29 1986-06-04 Leaback D H Bound assay components
FR2644893B1 (en) * 1989-03-24 1991-07-12 Sanofi Sa DOSAGE KIT AND METHOD APPLICABLE TO WHOLE CELLS
FR2644894B1 (en) * 1989-03-24 1994-05-13 Sanofi ENZYMATIC DOSAGE KIT AND METHOD APPLICABLE TO WHOLE CELLS
GB2289945B (en) * 1993-01-21 1997-06-18 Secr Defence Detecting microorganisms by assaying for ATP produced by intracellular adenylate kinase
GB9301118D0 (en) * 1993-01-21 1993-03-10 Secr Defence Enzyme linked assays
US5648232A (en) * 1993-01-21 1997-07-15 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Microbiological best method and reagents
US5658749A (en) * 1994-04-05 1997-08-19 Corning Clinical Laboratories, Inc. Method for processing mycobacteria
ES2171457T3 (en) * 1994-07-13 2002-09-16 Secr Defence Brit METHOD AND REAGENT FOR MICROBIOLOGICAL TEST.
CN1091804C (en) * 1994-07-13 2002-10-02 大不列颠及北爱尔兰联合王国国防大臣 Microbiological test method and reagents
US5744320A (en) * 1995-06-07 1998-04-28 Promega Corporation Quenching reagents and assays for enzyme-mediated luminescence
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3745090A (en) * 1970-08-04 1973-07-10 Nasa Method of detecting and counting bacteria in body fluids
FR2393370B1 (en) * 1977-05-31 1980-09-19 Inst Nat Sante Rech Med APPARATUS FOR ACQUIRING AND PRETREATING ELECTROCARDIOGRAPHIC OR VECTOCARDIOGRAPHIC DATA

Also Published As

Publication number Publication date
JPS57131063A (en) 1982-08-13
JPS6232421B2 (en) 1987-07-14
EP0054676A3 (en) 1982-08-04
EP0054676B1 (en) 1983-11-02
EP0054676A2 (en) 1982-06-30
ATE5201T1 (en) 1983-11-15
DE3047860A1 (en) 1982-07-15

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee