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DE1210514B - Process for the production of moenomycin - Google Patents

Process for the production of moenomycin

Info

Publication number
DE1210514B
DE1210514B DEF41297A DEF0041297A DE1210514B DE 1210514 B DE1210514 B DE 1210514B DE F41297 A DEF41297 A DE F41297A DE F0041297 A DEF0041297 A DE F0041297A DE 1210514 B DE1210514 B DE 1210514B
Authority
DE
Germany
Prior art keywords
moenomycin
production
fats
carbon source
streptomycetes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DEF41297A
Other languages
German (de)
Inventor
Dr Heinz Oeppinger
Dr Max Koernlein
Dr Georg Nesemann
Dr Adolf Oppermann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to BE655815D priority Critical patent/BE655815A/xx
Application filed by Hoechst AG filed Critical Hoechst AG
Priority to DEF41297A priority patent/DE1210514B/en
Priority to AT960264A priority patent/AT255649B/en
Priority to NL6413323A priority patent/NL6413323A/xx
Priority to GB46631/64A priority patent/GB1083546A/en
Publication of DE1210514B publication Critical patent/DE1210514B/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
    • C07G11/00—Antibiotics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

BUNDESREPUBLIK DEUTSCHLANDFEDERAL REPUBLIC OF GERMANY

DEUTSCHESGERMAN

PATENTAMTPATENT OFFICE

AUSLEGESCHRIFTEDITORIAL

Int. α.:Int. α .:

C12dC12d

Deutsche Kl.: 30h-6German class: 30h-6

Nummer: 1210 514Number: 1210 514

Aktenzeichen: F 41297IV a/30 hFile number: F 41297IV a / 30 h

Anmeldetag: 16. November 1963Filing date: November 16, 1963

Auslegetag: 10. Februar 1966Opening day: February 10, 1966

Nach der deutschen Patentschrift 1113 791 bilden Streptomyces ghanaensis und einige andere Streptomyceten eine antibiotisch wirksame Substanz, das Moenomycin. In der genannten Patentschrift wird angegeben, daß die Fermentation unter aeroben Bedingungen in einem wäßrigen Medium, das neben anorganischen Salzen als Kohlenstoffquelle Stärke, Glucose, Rohrzucker oder Melasse sowie als Stickstoffquelle Sojaschrot, Cornsteepliquor, Hefeextrakt, Baumwollsamen-, Keimlingsmehl Nitrate oder Ammoniumsalze enthält, bis zur Erreichung einer maximalen antibiotischen Aktivität durchgeführt wird. Im Beispiel wird eine Nährlösung aus 4% Stärkezucker, 3 4% Sojaschrot und 0,25% NaCl angegeben.Form according to German patent specification 1113 791 Streptomyces ghanaensis and some other Streptomycetes an antibiotic substance that Moenomycin. In the cited patent it is stated that the fermentation takes place under aerobic conditions in an aqueous medium which, in addition to inorganic salts as a carbon source, contains starch, Glucose, cane sugar or molasses and, as a nitrogen source, soy meal, cornsteel liquor, yeast extract, Cottonseed or sprout meal contains nitrates or ammonium salts, up to a maximum antibiotic activity is carried out. In the example, a nutrient solution made of 4% starch sugar, 3 4% soy meal and 0.25% NaCl indicated.

Es wurde nun gefunden, daß die Moenomycinbildung der obengenannten Streptomyceten ganz erheblich gesteigert werden kann, wenn als Kohlenstoffquelle tierische oder pflanzliche Fette eingesetzt werden. Die Fette können als alleinige Kohlenstoffquelle und auch zusammen mit anderen Kohlenstoffquellen, wie z. B. Zucker, eingesetzt werden. Bewährt haben sich als tierische Fette z. B. Tierkörperfett oder Schweineschmalz in Konzentrationen von 0,1 bis 10,0%, vorzugsweise 0,5 bis 5,0%. Als pflanzliche Fette kommen Öle, ζ. B. Sojabohnenöl, in Frage. Die Moenomycinbildung wurde dadurch von 300 bis 400 E/ml auf 700 bis 800 E/ml erhöht.It has now been found that the moenomycin formation of the abovementioned Streptomycetes completely can be increased significantly if animal or vegetable fats are used as the carbon source will. The fats can be used as the sole carbon source and also together with other carbon sources, such as B. sugar can be used. Have proven useful as animal fats z. B. carcass fat or lard in concentrations of 0.1 to 10.0%, preferably 0.5 to 5.0%. The vegetable fats are oils, ζ. B. soybean oil in question. The moenomycin formation was thereby increased from 300 to 400 U / ml to 700 to 800 U / ml.

Für Mikroorganismen sind Fette gewöhnlich schwerer verdaulich als Kohlehydrate. Es mußte erwartet werden, daß bei Verwendung von Fetten als Kohlenstoffquelle die Ausbeute an Moenomycin geringer ist als bei Verwendung von Kohlehydraten. Es war daher überraschend und nicht zu erwarten, daß bei Verwendung von Fetten die Moenomycinbildung sehr wesentlich erhöht wird.For microorganisms, fats are usually more difficult to digest than carbohydrates. It had to be expected be that when using fats as a carbon source, the yield of moenomycin is lower than when using carbohydrates. It was therefore surprising and not to be expected in use Moenomycin formation is increased very significantly by fats.

Beispiel 1example 1

Eine Kultur von S. ghanaensis wurde auf eine sterile Kulturlösung, bestehend aus 2% Sojaschrot, 0,5% Baumwollsamenmehl, 0,5% Cornsteepliquor, 0,25 % NaCl, 0,25% NaNO3, 0,05% KH2PO4, 1,5% Tierkörperfett, 0,5% Sojaöl, geimpft und unter Verfahren zur Herstellung von MoenomycinA culture of S. ghanaensis was on a sterile culture solution consisting of 2% soy meal, 0.5% cottonseed flour, 0.5% cornsteel pliquor, 0.25% NaCl, 0.25% NaNO 3 , 0.05% KH 2 PO 4 , 1.5% carcass fat, 0.5% soybean oil, inoculated and under process for making moenomycin

Anmelder:Applicant:

Farbwerke Hoechst AktiengesellschaftFarbwerke Hoechst Aktiengesellschaft

vormals Meister Lucius & Brüning, Frankfurt/M.formerly Master Lucius & Brüning, Frankfurt / M.

Als Erfinder benannt:
Dr. Heinz Öppinger, Hofheim (Taunus);
Dr. Max Körnlein, Frankfurt/M.-Höchst;
Dr. Georg Nesemann, Lorsbach (Taunus);
Dr. Adolf Oppermann, Hofheim (Taunus)Named as inventor:
Dr. Heinz Öppinger, Hofheim (Taunus);
Dr. Max Körnlein, Frankfurt / M.-Höchst;
Dr. Georg Nesemann, Lorsbach (Taunus);
Dr. Adolf Oppermann, Hofheim (Taunus)

intensivem Rühren und Belüftung 117 Stunden bei 32° C fermentiert. Es wurden in dieser Zeit 770 Einao heiten Moenomycin je Milliliter gebildet.Fermented at 32 ° C for 117 hours with intensive stirring and aeration. During this time there were 770 Einao units of moenomycin per milliliter.

Beispiel 2Example 2

Eine Kultur von S. bambergensis wurde auf eine sterile Kulturlösung folgender Zusammensetzung geimpft: 2% Sojaschrot, 0,5% Baumwollsamenmehl, 0,5% Cornsteepliquor, 0,25 % NaCl, 0,25% NaNO3, 0,05% KH2PO4, 1,5% Tierkörperfett, 0,5% Sojaöl, 1,0% Saccharose. Unter intensivem Rühren und Belüftung wurde 120 Stunden bei 32° C fermentiert. Es wurden 765 Einheiten Moenomycin je Milliliter gebildet. A culture of S. bambergensis was inoculated on a sterile culture solution of the following composition: 2% soy meal, 0.5% cottonseed meal, 0.5% cornsteel liquor, 0.25% NaCl, 0.25% NaNO 3 , 0.05% KH 2 PO 4 , 1.5% carcass fat, 0.5% soybean oil, 1.0% sucrose. Fermentation was carried out at 32 ° C. for 120 hours with intensive stirring and aeration. 765 units of moenomycin were formed per milliliter.

Claims (1)

Patentanspruch:Claim: Verfahren zur Herstellung von Moenomycin durch mikrobiologische Fermentation mit einem moenomycinbildenden Streptomyceten, dadurch gekennzeichnet, daß als Kohlenstoffquelle tierische und/oder pflanzliche Fette eingesetzt werden.Process for the production of moenomycin by microbiological fermentation with a moenomycin-forming streptomycetes, characterized in that the carbon source animal and / or vegetable fats are used. In Betracht gezogene Druckschriften:
Deutsche Auslegeschrift Nr. 1113 791.Considered publications:
German publication No. 1113 791.
DEF41297A 1963-11-16 1963-11-16 Process for the production of moenomycin Pending DE1210514B (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
BE655815D BE655815A (en) 1963-11-16
DEF41297A DE1210514B (en) 1963-11-16 1963-11-16 Process for the production of moenomycin
AT960264A AT255649B (en) 1963-11-16 1964-11-13 Process for the production of moenomycin
NL6413323A NL6413323A (en) 1963-11-16 1964-11-16
GB46631/64A GB1083546A (en) 1963-11-16 1964-11-16 Process for the manufacture of moenomycin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEF41297A DE1210514B (en) 1963-11-16 1963-11-16 Process for the production of moenomycin

Publications (1)

Publication Number Publication Date
DE1210514B true DE1210514B (en) 1966-02-10

Family

ID=7098610

Family Applications (1)

Application Number Title Priority Date Filing Date
DEF41297A Pending DE1210514B (en) 1963-11-16 1963-11-16 Process for the production of moenomycin

Country Status (5)

Country Link
AT (1) AT255649B (en)
BE (1) BE655815A (en)
DE (1) DE1210514B (en)
GB (1) GB1083546A (en)
NL (1) NL6413323A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3992263A (en) * 1974-07-23 1976-11-16 Hoechst Aktiengesellschaft Process for the preparation of moenomycin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1113791B (en) * 1960-02-10 1961-09-14 Hoechst Ag Production and extraction of the antibiotic moenomycin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1113791B (en) * 1960-02-10 1961-09-14 Hoechst Ag Production and extraction of the antibiotic moenomycin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3992263A (en) * 1974-07-23 1976-11-16 Hoechst Aktiengesellschaft Process for the preparation of moenomycin

Also Published As

Publication number Publication date
AT255649B (en) 1967-07-10
GB1083546A (en) 1967-09-13
NL6413323A (en) 1965-05-17
BE655815A (en)

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