CZ9106U1 - Therapeutical compositions for treating early postnatal disorders and mastitis of domestic animals - Google Patents

Description

Medicinal products for early postnatal problems and mastitis in livestock

Technical field

The technical solution relates to veterinary preparations for prevention and treatment of early postnatal problems of animals, in particular diarrheal diseases of pups, traumatic lesions of the uterine and vaginal mucosa of females after delivery and mastitis.

BACKGROUND OF THE INVENTION

At present, probiotic products are used only in a limited indication range (oral application for the prevention or treatment of diarrheal diseases in the crown postnatal period). For these indications, cultures of Streptococcus, Enterococcus, E. coli, Lactobacillus are used in various combinations and concentrations (Ecovit, Florivit, Lactiferm, Probios, Equibion and others) between these individual bacterial cultures, possibly bacterial cultures of Enterococcus supplemented with a different sequence of vitamin supplements.

Lactobacilllus strains have not been used at all for gynecological indications in veterinary practice, nor for intramammary application. To date, only antibiotic drugs have been applied in these cases.

The essence of the technical solution

The technical solution consists in the composition of veterinary preparations for early postnatal problems and mastitis of farm animals, which in an inert vehicle per 1 g of Lactobacillus acidophilus culture with a number of living organisms 10 ⁶ to 10 ⁹ contain 8000 to 30 000 IU. retinol or a veterinarily acceptable salt thereof. In an embodiment for oral administration, in particular for the prevention and treatment of juvenile diarrhea, preferably 150 to 300 mg of ascorbic acid per g of said culture is further included. Thus, a medicament for reducing losses in piglets per 1 g of Lactobacillus acidophilus culture with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 15,000 to 30,000 IU of retinol palmitate, 150 to 300 mg of ascorbic acid and arachidonic oil to 10 ml. The medicament for loss reduction in calves is preferably in the form of tablets and per 1 g culture of Lactobacillus acidophilus with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 8000 to 15,000 IU of retinol acetate, 150 to 300 mg ascorbic acid and 5 to g. In an embodiment for intrauterine and intramammary administration, in particular for the treatment of traumatic lesions after delivery and mastitis in females, the composition additionally preferably contains 2 to 10 mg of digestive enzyme per g of said culture. The medicament for the treatment of traumatic lesions of the vaginal and uterine mucosa after delivery thus preferably per 2 g of a culture of Lactobacillus acidophilus with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 4 to 6 mg chymotrypsin, 30,000 to 50,000 IU retinol palmitate and oil to 20 ml. The medicament for the treatment of post-partum mastitis preferably per 1 g of 10 ⁶ to 10 ⁸ Lactobacillus acidophilus culture contains 4 to 6 mg chymotrypsin, 1 to 4 mg trypsin, 8000 to 15 000 IU retinol palmitate and oil up to 10 ml .

The preparation according to the invention consists of a combination of Lactobacillus strains not yet used in combination with vitamins for the above mentioned indications, ie postnatal coli-sepsis, diarrhea, etc.

The beneficial effects of the preparation according to the invention for early postnatal problems and mastitis in livestock use the properties of vitamins A and C and the characteristics of the Lactobacillus strain. Vitamin A (retinol) has beneficial effects on the protection and function of mucous membranes, vitamin C (ascorbic acid) works on the basis of the oxidation-reducing potential, and finally the Lactobacillus strain acts as a dominant bacterium that prevents its growth by pathogenic or conditionally pathogenic bacteria. metabolites have a beneficial effect on mucosal protection.

-1 GB 9106 U1

Lactobacilllus strains have not been used at all for gynecological indications in veterinary practice, nor for intramammary application. In these cases, the product can only be compared with antibiotic drugs.

Intrauterine applications utilize the properties of the Lactobacillus strain together with vitamin A and the digestive enzyme. The use of the digestive enzyme results in lysis and faster evacuation of cellular detritus following traumatic lesions of the uterine and vaginal mucosa.

For intramammary application, rapid growth of the Lactobacillus strain is utilized, thereby preventing the growth of secondary microflora in mastitis, utilizing the lytic effect of digestive enzymes on already bacterially altered milk secretions and utilizing the protective function of vitamin A on the mucosal outlet of the mammary gland system.

In the latter two applications (intrauterine, intramammary), the advantage of using these preparations, in comparison with antibiotic drugs, is obvious: low burden on the organism with foreign substances, furthermore it is not necessary to exclude milk from the supply to dairies, which implies economical advantage for breeders. The normal period of withdrawal of milk is 6 to 9 days (including treatment).

Examples

Example 1

Oral suspension for loss reduction in pigs is prepared from the following components:

Lactobacillus acidophilus 3 x 10 ⁸ 1 g retinol palmitate 25 000 IU

ascorbic acid 150 mg vehicle (arachidone oil) ad 10 ml.

The suspension is applied strictly on an individual basis when weaning piglets (approx. 25 kg body weight) per os 2 ml.

Example 2

Oral loss reduction tablets for calves are prepared from the following ingredients:

Lactobacillus acidophilus 10 ⁸ 1 g retinol acetate 10 000 IU

ascorbic acid 150 mg vehicle (sucrose) ad 5 g.

Dosage: 1 tablet on the 1st and 10th day after birth to calves (approx. 30 kg bodyweight).

Example 3

The intrauterine suspension is prepared from the following components:

Lactobacillus acidophilus 10 ⁸ 2 g chymotrypsin 5 mg retinol palmitate 45 000 IU

vehicle (oil) ad 20 ml.

Example 4

Intramammary suspensions are prepared from the following components:

Lactobacillus acidophilus 10 ⁶ 1 g

9106 U1 chymotrypsin 5 mg trypsin 3 mg retinol palmitate 10,000 IU.

vehicle (oil) ad 10 ml.

Efficacy of Lactobacillus acidophilus:

Intrauterine applications:

Even in very severe cases, such as prolapse of the uterus after delivery in cows, after severe births in cows, the healing of damaged tissues has been satisfactorily achieved.

When applied in both preventive and therapeutic sows, when purulent outflow from the external genitalia due to endometritis occurred on the days 4-6 of post partitions, this phenomenon was not observed even in cases of prolonged labor.

When the uterus was prolapsed at the mare, the tissue repair was such that the mare was admitted to the post part on day 9.

Intramammary applications:

The results of the cows after delivery were satisfactory after one or two applications the udder was without inflammation before the withdrawal period, the milk could be immediately included in the delivery.

Prevention and treatment of infant diarrhea:

Calves still commonly develop diarrhea on the 4th to 6th day after birth, which can be fatal. After administration of one tablet on the first day after delivery, diarrhea in calves only occurred on day 11, when the calf already had enough antibodies.

In piglets the situation is similar on the 5th day after weaning. Here, individual application is necessary, where farm workers manipulate piglets (transport to the rearing house), therefore there is no problem to apply per os. In nurseries where the piglets are approximately twenty pieces in a pen, when using conventional dry feed preparations, about 12-20% of the weaker individuals do not get on time to feed, resulting in a lower pulvis effect on feeding. Individual oral administration of the suspension according to the invention has achieved an improvement in the condition over a period of fifteen days, which is already sufficient to acclimatize the individual and to cope with post-weaning stress. The use of vitamins A and C increases the anti-stress effect.

Claims (7)

PROTECTION REQUIREMENTS Medicament for early postnatal problems and mastitis in livestock, characterized in that in an inert vehicle per 1 g of Lactobacillus acidophilus culture with a number of living organisms of 10 ^{3 * * 6} to 10 ^{9 it} contains 8000 to 30 000 IU retinol or its veterinarily acceptable salts. Medicament according to claim 1, in particular for the prevention and treatment of juvenile diarrhea, characterized in that it further comprises 150 to 300 mg of ascorbic acid per g of said culture. Medicament according to claim 2, in particular for reducing losses in piglets, characterized in that, per 1 g of Lactobacillus acidophilus culture by count of living organisms 1.10 ⁸ to 4.10 ^{8, it} contains 15 000 to 30 000 IU retinol palmitate, 150 to 300 mg ascorbic acid and as an inert vehicle arachidone oil. -3 GB 9106 U1 Medicament according to claim 2, in particular for reducing losses in calves, characterized in that, per 1 g of Lactobacillus acidophilus culture by count of living organisms 1.10 ⁸ to 4.10 ^{8, it} contains 8000 to 15 000 IU retinol acetate, 150 to 300 mg ascorbic acid and as an inert vehicle of sucrose. Medicament according to claim 1, in particular for the treatment of traumatic lesions after delivery and mastitis in females, characterized in that it further comprises 2 to 10 mg of digestive enzyme per g of said culture. Medicament according to claim 5, in particular for the treatment of traumatic lesions of the vaginal and uterine mucosa after delivery, characterized in that a woman of Lactobacillus acidophilus 10 with an organism number of 1.10 ⁸ to 8.10 ⁸ contains 4 to 6 mg chymotrypsin, 30 000 to 50 000 inter alia retinol palmitate and as an inert vehicle an oil. Medicament according to claim 5, in particular for the treatment of post-partum mastitis, characterized in that it contains from 4 to 6 mg chymotiypsine, from 1 to 4 mg of tiypsine, from 8000 to 15 per 1 g of Lactobacillus acidophilus with a number of living organisms of 10 ⁶ to 10 ^8. 000 IU retinol palmitate 15 and j as an inert vehicle oil.