CZ288454B6 - Healing preparations for early postnatal problems and mastitis of domestic animals - Google Patents

Abstract

The invented preparations contain in an inert vehicle 8000 to 30 000 m.u. of retinol or veterinary acceptable salts thereof per 1 g of Lactobacillus acidophilus culture with vial organisms in the amount ranging from 10e6 to 10e9. In order to reduce losses of young stock the preparations further contain 150 to 300 mg of ascorbic acid and for treating mastitis and after-lesions of vaginal mucous membrane and endometrium in females the preparation contain in addition thereto digestive enzymes in amount ranging from 2 to 10 mg.

Description

Medicinal products for early postnatal problems and mastitis in livestock

Technical field

The invention relates to veterinary preparations for the prevention and treatment of early postnatal problems of animals, in particular diarrheal diseases of pups, traumatic lesions of the uterine and vaginal mucosa of post-natal females and mastitis.

BACKGROUND OF THE INVENTION

At present, probiotic products are used only in a limited indication range (oral application for prevention or treatment of diarrheal diseases of animal categories in the early postnatal period). For these indications, Streptococcus, Enterococcus, E. coli, Lactobacillus cultures are used in various combinations and concentrations (Ecovit, Florivit, Lactiferm, Probios, Equibion and others) between these individual bacterial cultures, ev. Enterococcus bacterial cultures supplemented with a different sequence of vitamin supplements.

Lactobacillus strains have not been used at all for gynecological indications in veterinary practice, nor for intramammary application. To date, only antibiotic drugs have been applied in these cases.

SUMMARY OF THE INVENTION

The invention consists of veterinary preparations for early postnatal problems and mastitis of livestock which, in an inert vehicle per 1 g of Lactobacillus acidophilus culture with a number of living organisms of 10 ⁶ to 10 ^9, contain 8000 to 30 000 IU retinol or its veterinarily acceptable salts. In an embodiment for oral administration, in particular for the prevention and treatment of juvenile diarrhea, preferably 150 to 300 mg of ascorbic acid per g of said culture is further included. Thus, a medicament for reducing losses in piglets per 1 g of Lactobacillus acidophilus culture with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 15,000 to 30,000 IU of retinol palmitate, 150 to 300 mg of ascorbic acid and arachidonic oil to 10 ml. The medicament for loss reduction in calves is preferably in the form of tablets and per 1 g of Lactobacillus acidophilus culture with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 8000 to 15,000 IU retinol acetate, 150 to 300 mg ascorbic acid and sucrose to 5 g. an embodiment for intrauterine and intramammary administration, in particular for the treatment of traumatic lesions after delivery and mastitis in females, the formulation preferably comprises 2 to 10 mg digestive enzyme per g of said culture. The medicament for the treatment of traumatic lesions of the vaginal and uterine mucosa after delivery thus preferably per 2 g of a culture of Lactobacillus acidophilus with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 4 to 6 mg chymotrypsin, 30,000 to 50,000 IU retinol palmitate and oil to 20 ml. The medicament for the treatment of post-partum mastitis preferably per 1 g of 10 ⁶ to 10 ⁸ Lactobacillus acidophilus culture contains 4 to 6 mg chymotrypsin, 1 to 4 mg trypsin, 8000 to 15 000 IU retinol palmitate and oil to 10 ml.

The formulation according to the invention is composed of a combination of Lactobacillus strains not yet used in combination with vitamins for the above indications, ie postnatal coli-sepsis, diarrhea, etc.

The beneficial effects of the composition of the invention for early postnatal problems and mastitis in livestock use the properties of vitamins A and C and the properties of the Lactobacillus strain. Vitamin A (retinol) has beneficial effects on the protection and function of mucous membranes, vitamin C (ascorbic acid) works on the basis of the redox potential and finally the Lactobacillus strain acts as

-1C 288454 B6 a dominant bacterium that prevents the growth of pathogenic ev. conditionally pathogenic strains of bacteria, and its metabolites have a beneficial effect on mucosal protection.

Lactobacillus strains have not been used at all for gynecological indications in veterinary practice, nor for intramammary application. In these cases, the product can only be compared with antibiotic drugs.

Intrauterine applications utilize the properties of the Lactobacillus strain together with vitamin A and the digestive enzyme. The use of the digestive enzyme results in lysis and faster evacuation of cellular detritus following traumatic lesions of the uterine and vaginal mucosa.

The intramammary application utilizes rapid multiplication of the Lactobacillus strain, thereby preventing secondary microflora growth in mastitis, utilizing the lyrical effect of digestive enzymes on already bacterially altered milk secretions and utilizing the protective function of vitamin A on the mucosa of the mammary gland system.

In the latter two applications (intrauterine, intramammary), the advantage of using these preparations, in comparison with antibiotic drugs, is obvious: low burden on the organism with foreign substances, furthermore it is not necessary to exclude milk from the supply to dairies, which implies economical advantage for breeders. The normal time to remove milk from delivery without using the composition of the invention is 6 to 9 days (including treatment).

Examples

Example 1

Oral suspension for loss reduction in pigs is prepared from the following components:

Lactobacillus acidophilus 3x10 ⁸ retinol palmitate ascorbic acid vehicle (arachidone oil) 1 g 25 000 IU 150 mg ad 10 ml

The suspension is applied strictly individually when weaning piglets (approx. 25 kg bw) per os 2 ml.

Example 2

Oral loss reduction tablets for calves are prepared from the following ingredients:

Lactobacillus acidophilus 10 ⁸ Retinol acetate Ascorbic acid Vehicle (sucrose) 1 g 10 000 IU 150 mg and 5 g

Dosage: 1 tbl. 1 and 10 days after birth calves (approx. 30 kg of body weight).

-2GB 288454 B6

Example 3

The intrauterine suspension is prepared from the following components:

Lactobacillus acidophilus 10 ⁸ chymotrypsin retinol palmitate vehicle (oil)

2g mg

000 IU ad 20 ml

Example 4

Intramammary suspensions are prepared from the following components:

Lactobacillus acidophilus 10 ⁶ chymotrypsin trypsin retinol palmitate vehicle (oil) g

mg mg

000 IU ad 10 ml

Activity of the Lactobacillus acidophilus strains according to the invention:

Intrauterine applications:

Even in very severe cases, such as prolapse of the uterus after delivery in cows, after severe births in cows, the healing of damaged tissues has been satisfactorily achieved.

When applied in both preventive and therapeutic sows, when purulent outflow from the external genitalia due to endometritis occurred on the days 4-6 of post partitions, this phenomenon was not observed even in prolonged deliveries.

When the uterus was prolapsed at the mare, the tissue repair was such that the mare was admitted to the post part on day 9.

Intramammary applications:

The results in post-partum cows were satisfactory after one or two applications - the udder was without inflammation before the withdrawal period and the milk could be included immediately.

Prevention and treatment of infant diarrhea:

Calves still commonly develop diarrhea on the 4th to 6th day after birth, which can be fatal. After administration of one tablet on the first day after delivery, diarrhea in calves only occurred on day 11, when the calf already had enough antibodies.

In piglets the situation is similar on the 5th day after weaning. Here, individual application is necessary, where farm workers manipulate piglets (transport to the rearing house), therefore there is no problem to apply per os. In nurseries where the piglets are approximately twenty pieces in a pen, when using conventional dry feed preparations, about 12-20% of the weaker individuals do not get on time to feed, resulting in a lower pulvis effect on feeding. Individual oral administration of the suspension of the invention has achieved an improvement in the condition over a period of fifteen days, which is already sufficient to acclimatize the individual and cope with post-weaning stress. The use of vitamins A and C increases the anti-stress effect.

Claims (7)

PATENT CLAIMS Medicaments for early postnatal problems and livestock mastitis, characterized in that in an inert vehicle per 1 g of Lactobacillus acidophilus culture with a number of live organisms of 10 ⁶ to 10 ^{9, they} contain 8000 to 30 000 IU of retinol or veterinarily acceptable salts thereof. Medicaments according to claim 1, in particular for the prevention and treatment of infant diarrhea, characterized in that they further comprise 150 to 300 mg of ascorbic acid per g of said culture. Medicament according to claim 2, in particular for reducing losses in piglets, characterized in that, per 1 g of Lactobacillus acidophilus culture by count of living organisms 1.10 ⁸ to 4.10 ^{8, it} contains 15 000 to 30 000 IU retinol palmitate, 150 to 300 mg ascorbic acid and as an inert vehicle arachidone oil. Medicament according to claim 2, in particular for reducing losses in calves, characterized in that, for 1 g of a culture of Lactobacillus acidophilus with a number of living organisms of 1.10 * to 4.10 ^{8, it} contains 8000 to 15 000 IU retinol acetate, 150 to 300 mg ascorbic acid and as an inert vehicle sucrose. Medicaments according to claim 1, in particular for the treatment of traumatic lesions after delivery and mastitis in females, characterized in that they further comprise 2 to 10 mg of digestive enzyme per g of said culture. Medicament according to claim 5, in particular for the treatment of traumatic lesions of the vaginal and uterine mucosa after delivery, characterized in that a woman of Lactobacillus acidophilus culture with a number of living organisms of 1.10 ⁸ to 4.10 ⁸ contains 4 to 6 mg chymotiypsine, 30,000 to 50,000 IU retinol palmitate and as an inert vehicle an oil. Medicament according to claim 5, in particular for the treatment of post-partum mastitis, characterized in that it contains 4 to 6 mg chymotiypsine, 1 to 4 mg trypsin, 8000 to 15 per 1 g of Lactobacillus acidophilus by counting living organisms 10 ⁶ to 10 ⁸ 000 IU of retinol palmitate and as an inert vehicle oil.