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CY1112209T1 - Διαδικασια για την αναδιαταξη των ανασυνδυασμενων πρωτεϊνων που περιεχουν δισουλφιδιο, σε υψηλες συγκεντρωσεις πρωτεϊνων με την παρουσια αμινων - Google Patents

Διαδικασια για την αναδιαταξη των ανασυνδυασμενων πρωτεϊνων που περιεχουν δισουλφιδιο, σε υψηλες συγκεντρωσεις πρωτεϊνων με την παρουσια αμινων

Info

Publication number
CY1112209T1
CY1112209T1 CY20111100332T CY111100332T CY1112209T1 CY 1112209 T1 CY1112209 T1 CY 1112209T1 CY 20111100332 T CY20111100332 T CY 20111100332T CY 111100332 T CY111100332 T CY 111100332T CY 1112209 T1 CY1112209 T1 CY 1112209T1
Authority
CY
Cyprus
Prior art keywords
reproduction
procedure
high protein
protein concentrates
proteins
Prior art date
Application number
CY20111100332T
Other languages
English (en)
Inventor
Jörg Peters
Torsten Minuth
Original Assignee
Bayer Pharma Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Pharma Aktiengesellschaft filed Critical Bayer Pharma Aktiengesellschaft
Publication of CY1112209T1 publication Critical patent/CY1112209T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5406—IL-4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Μία μέθοδος για την αναδιάταξη των πρωτεϊνών που περιλαμβάνει την προσθήκη ενός ρυθμιστικού διαλύματος αναδίπλωσης, που περιέχει μια πρωτοταγή, δευτεροταγή ή τριτοταγή αμίνη, σε ένα διάλυμα από μετουσιωμένες, χημικά τροποποιημένες ή ανηγμένες πρωτεΐνες. Η εν λόγω μέθοδος έχει εφαρμοστεί, για παράδειγμα, σε ιντερλευκίνη-4 και αναστολέα της παγκρεατικής θρυψίνης βοοειδών (ΒΡΤΙ), τα οποία είχαν προηγουμένως (i) διαλυτοποιηθεί με την παρουσία της υδροχλωρικής γουανιδίνης ως χαοτροπικό μέσο και (ii) είχαν υποστεί διάσπαση με θειούχα ιόντα.
CY20111100332T 2001-11-22 2011-03-29 Διαδικασια για την αναδιαταξη των ανασυνδυασμενων πρωτεϊνων που περιεχουν δισουλφιδιο, σε υψηλες συγκεντρωσεις πρωτεϊνων με την παρουσια αμινων CY1112209T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01127373A EP1314739A1 (en) 2001-11-22 2001-11-22 Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines
EP02774777A EP1453858B1 (en) 2001-11-22 2002-11-12 Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines

Publications (1)

Publication Number Publication Date
CY1112209T1 true CY1112209T1 (el) 2015-12-09

Family

ID=8179272

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111100332T CY1112209T1 (el) 2001-11-22 2011-03-29 Διαδικασια για την αναδιαταξη των ανασυνδυασμενων πρωτεϊνων που περιεχουν δισουλφιδιο, σε υψηλες συγκεντρωσεις πρωτεϊνων με την παρουσια αμινων

Country Status (12)

Country Link
US (1) US7893210B2 (el)
EP (2) EP1314739A1 (el)
JP (1) JP4348188B2 (el)
KR (1) KR100944845B1 (el)
AT (1) ATE495191T1 (el)
AU (1) AU2002340510A1 (el)
CY (1) CY1112209T1 (el)
DE (1) DE60238938D1 (el)
DK (1) DK1453858T3 (el)
ES (1) ES2357888T3 (el)
PT (1) PT1453858E (el)
WO (1) WO2003044055A1 (el)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7442370B2 (en) 2001-02-01 2008-10-28 Biogen Idec Ma Inc. Polymer conjugates of mutated neublastin
WO2006023782A2 (en) 2004-08-19 2006-03-02 Biogen Ideca Ma Inc. Refolding transforming growth factor beta family proteins
TWI501774B (zh) 2006-02-27 2015-10-01 Biogen Idec Inc 神經性病症之治療
JP4984983B2 (ja) * 2007-03-09 2012-07-25 富士通株式会社 符号化装置および符号化方法
JP5583005B2 (ja) 2007-05-01 2014-09-03 バイオジェン・アイデック・エムエイ・インコーポレイテッド 血管新生を増大させるための組成物および方法
WO2016081289A1 (en) * 2014-11-18 2016-05-26 Merck Sharp & Dohme Corp. Process for producing recombinant trypsin
CN113322222A (zh) * 2021-07-02 2021-08-31 江苏恒丰强生物技术有限公司 一种表达猫ω干扰素的基因工程菌的生产工艺

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Publication number Priority date Publication date Assignee Title
US5453363A (en) 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
WO1989001046A1 (en) * 1987-07-29 1989-02-09 Schering Biotech Corporation Purification of human interleukin-4 expressed in escherichia coli
AU3350893A (en) * 1991-07-29 1993-03-02 Immunex Corporation Process for isolating and purifying recombinant interleukin-7
DE4137333A1 (de) 1991-11-13 1993-05-19 W Prof Dr Sebald Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
ATE189004T1 (de) * 1994-10-13 2000-02-15 Kyowa Medex Co Ltd Verfahren zur bestimmung von harnstoff stickstoff
US5563057A (en) 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5728804A (en) 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
ATE358684T1 (de) * 1995-06-07 2007-04-15 Novartis Vaccines & Diagnostic Waessrige formulierung enthaltend tfpi und lösungsvermittlers
WO1998020033A2 (en) * 1996-11-06 1998-05-14 Schering Corporation RENATURATION AND PURIFICATION OF VIRAL INTERLEUKIN-10 (vIL-10)
WO2001087925A2 (en) * 2000-05-16 2001-11-22 Bolder Biotechnology, Inc. Methods for refolding proteins containing free cysteine residues
US20020052026A1 (en) * 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
KR100253916B1 (ko) * 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
DE69929638T2 (de) 1999-01-20 2006-09-21 Bayer Healthcare Ag Plasmide, deren Konstruktion und Anwendung zur Herstellung von Interleukin-4 und dessen Muteine
GB9912350D0 (en) 1999-05-26 1999-07-28 European Molecular Biology Lab Embl Modified cytokine

Also Published As

Publication number Publication date
US7893210B2 (en) 2011-02-22
AU2002340510A1 (en) 2003-06-10
ES2357888T3 (es) 2011-05-03
PT1453858E (pt) 2011-03-16
DE60238938D1 (de) 2011-02-24
JP2005510217A (ja) 2005-04-21
ATE495191T1 (de) 2011-01-15
US20050014933A1 (en) 2005-01-20
EP1453858A1 (en) 2004-09-08
KR20050058243A (ko) 2005-06-16
WO2003044055A1 (en) 2003-05-30
EP1314739A1 (en) 2003-05-28
KR100944845B1 (ko) 2010-03-04
DK1453858T3 (da) 2011-05-09
EP1453858B1 (en) 2011-01-12
JP4348188B2 (ja) 2009-10-21

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