CN2395277Y - Combination Antigen Echinococcosis Rapid Diagnosis Reaction Panel - Google Patents
Combination Antigen Echinococcosis Rapid Diagnosis Reaction Panel Download PDFInfo
- Publication number
- CN2395277Y CN2395277Y CN99219078.9U CN99219078U CN2395277Y CN 2395277 Y CN2395277 Y CN 2395277Y CN 99219078 U CN99219078 U CN 99219078U CN 2395277 Y CN2395277 Y CN 2395277Y
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- China
- Prior art keywords
- antigen
- echinococcosis
- fiber film
- purified
- base
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 239000000427 antigen Substances 0.000 title claims abstract description 48
- 102000036639 antigens Human genes 0.000 title claims abstract description 48
- 108091007433 antigens Proteins 0.000 title claims abstract description 48
- 206010014096 Echinococciasis Diseases 0.000 title claims abstract description 16
- 208000009366 Echinococcosis Diseases 0.000 title claims abstract description 16
- 238000003745 diagnosis Methods 0.000 title claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 10
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 239000000835 fiber Substances 0.000 claims abstract description 13
- 201000003808 Cystic echinococcosis Diseases 0.000 claims abstract description 9
- 241000244170 Echinococcus granulosus Species 0.000 claims abstract description 8
- 210000002726 cyst fluid Anatomy 0.000 claims abstract description 8
- 241000244160 Echinococcus Species 0.000 claims abstract description 4
- 206010039722 scoliosis Diseases 0.000 claims abstract 2
- 239000013641 positive control Substances 0.000 claims description 8
- 230000002745 absorbent Effects 0.000 claims description 4
- 239000002250 absorbent Substances 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- 238000003748 differential diagnosis Methods 0.000 abstract description 2
- 238000013399 early diagnosis Methods 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical group [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 101710098119 Chaperonin GroEL 2 Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 101000894412 Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) Bacterioferritin Proteins 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 101000588258 Taenia solium Paramyosin Proteins 0.000 description 1
- 241000212749 Zesius chrysomallus Species 0.000 description 1
- 208000035472 Zoonoses Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 206010048282 zoonosis Diseases 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
一种组合抗原包虫病快速诊断反应板,其包括上盖和底座,在上盖上有检测孔,上盖的检测孔与底座之间有纤维薄膜,纤维薄膜上包被有四种抗原,第一种抗原为部分纯化细粒棘球蚴囊液抗原,第二种抗原为部分纯化细粒棘球蚴头节抗原,第三种抗原为纯化囊液抗原,第四种抗原为纯化的泡状棘球蚴抗原;其结构简单、操作简便,用于包虫病的早期诊断和鉴别诊断,使结果特异性强,灵敏度高,大大减少了漏诊率,一般需3分钟即查做出结果,故特别适用于样本的筛查。
A combined antigen echinococcosis rapid diagnosis reaction plate, which includes a top cover and a base, a detection hole is arranged on the top cover, a fiber film is arranged between the detection hole of the top cover and the base, and four kinds of antigens are coated on the fiber film, The first antigen is the partially purified Echinococcus granulosus cyst fluid antigen, the second antigen is the partially purified Echinococcus granulosus scoliosis antigen, the third antigen is the purified cyst fluid antigen, and the fourth antigen is the purified vesicle antigen. Echinococcus antigen; it has a simple structure and is easy to operate. It is used in the early diagnosis and differential diagnosis of echinococcosis, which makes the results highly specific and sensitive, and greatly reduces the rate of missed diagnosis. Generally, it takes only 3 minutes to get the results. Therefore, it is especially suitable for the screening of samples.
Description
本实用新型涉及用于诊断包虫病的反应板，是一种组合抗原包虫病快速诊断反应板。The utility model relates to a reaction board for diagnosing echinococcosis, which is a reaction board for rapid diagnosis of echinococcosis with combined antigens.
包虫病是一种流行于畜牧区的常见寄生虫病，属于严重危害人体健康的人畜共患病之一。中国西部、北部、中部及东北部分地区十一个省区均有报道，其中以新疆包虫病感染程度居全国之首。目前，常规包虫病实验室检验的主要方法为卡松尼试验、对流免疫电泳及间接血凝试验等，其假阳性及假阴性率均较高，并且操作复杂、测定时间较长。Hydatid disease is a common parasitic disease prevalent in livestock areas, and it is one of the zoonotic diseases that seriously endanger human health. It has been reported in 11 provinces in western, northern, central and northeastern parts of China, among which the degree of echinococcosis infection in Xinjiang ranks first in the country. At present, the main methods of routine echinococcosis laboratory testing are Cassonian test, convective immunoelectrophoresis and indirect hemagglutination test, etc., which have high false positive and false negative rates, complicated operation and long measurement time.
鉴于上述存在的问题，本实用新型的目的在于设计一种结构简单、操作方便的组合抗原包虫病快速诊断反应板。In view of the above problems, the purpose of this utility model is to design a combination antigen echinococcosis rapid diagnosis reaction plate with simple structure and convenient operation.
本实用新型的目的是通过以下措施来实现的：该组合抗原包虫病快速诊断反应板包括上盖和底座，在上盖上有检测孔，在上盖的检测孔与底座之间有纤维薄膜，在纤维薄膜上包被有四种不同的抗原，第一种抗原为部分纯化细粒棘球蚴囊液抗原，第二种抗原为部分纯化细粒棘球蚴头节抗原，第三种抗原为纯化囊液抗原，第四种抗原为纯化的泡状棘球蚴抗原。纤维薄膜与底座之间有吸水纸。纤维薄膜上包被有阳性控制点，而阳性控制点为人体血清。The purpose of this utility model is achieved by the following measures: the combined antigen echinococcosis rapid diagnosis reaction plate includes an upper cover and a base, a detection hole is arranged on the upper cover, and a fiber film is arranged between the detection hole and the base of the upper cover , four different antigens were coated on the fibrous film, the first antigen was the partially purified Echinococcus granulosus cyst fluid antigen, the second antigen was the partially purified Echinococcus granulosus scorchoid antigen, and the third antigen was To purify cyst fluid antigens, the fourth antigen is purified alveolar Echinococcus antigen. Absorbent paper is arranged between the fiber film and the base. Positive control points are coated on the fiber film, and the positive control points are human serum.
附图1为本实用新型最佳实施例的主视结构示意图，Accompanying drawing 1 is the front view structure schematic diagram of the preferred embodiment of the present utility model,
附图2为附图1的a-a向剖视结构示意图，Accompanying drawing 2 is the a-a sectional structure diagram of accompanying drawing 1,
附图中的编码分别为：1为上盖，2为底座，3为检测孔，4为纤维薄膜，5为吸水纸，6为记录片；A为第一种抗原，B为第二种抗原，C为第三种抗原，D为第四种抗原，E为阳性控制点。The codes in the drawings are: 1 is the upper cover, 2 is the base, 3 is the detection hole, 4 is the fiber film, 5 is the absorbent paper, 6 is the recording sheet; A is the first antigen, B is the second antigen, C is the third antigen, D is the fourth antigen, and E is the positive control point.
下面结合最佳实施例及附图对本实用新型作进一步描述：Below in conjunction with preferred embodiment and accompanying drawing, the utility model is further described:
如附图1和2所示，该组合抗原包虫病快速诊断反应板包括上盖1和底座2，在上盖1上有检测孔3，在上盖1的检测孔3与底座2之间有纤维薄膜4，在纤维薄膜42上包被四种不同的抗原，第一种抗原A为部分纯化细粒棘球蚴囊液抗原，第二种抗原B为部分纯化细粒棘球蚴头节抗原，第三种抗原C为纯化囊液抗原，第四种抗原D为纯化的泡状棘球蚴抗原。As shown in accompanying drawings 1 and 2, the combined antigen echinococcosis rapid diagnosis reaction plate comprises a loam cake 1 and a base 2, and a detection hole 3 is arranged on the loam cake 1, between the detection hole 3 of the loam cake 1 and the base 2 There is a fibrous membrane 4, and four different antigens are coated on the fibrous membrane 42, the first antigen A is the partially purified Echinococcus granulosus cyst fluid antigen, and the second antigen B is the partially purified Echinococcus granulosus scorch Antigens, the third antigen C is a purified cyst fluid antigen, and the fourth antigen D is a purified alveolar Echinococcus antigen.
使用时：首先，将本实用新型平放，取20μl血清或40μl全血置一离心管中，并滴入A液5滴(全血一分钟后滴入)，混匀(全血若产生混浊需要2000rpm离心2分钟)，取上述混合液上清3滴(或吸取100μl)，滴入本实用新型的检测孔3内；其次，滴3滴B液于检测孔3内，滴3滴C液于检测孔3内，滴3滴B液于检测孔3内；然后观察并记录结果，一般3分钟左右即可做出结果，阳性：“+”淡红色，“++”红色，“+++”紫红色，阴性：“-”A、B、C、D处无红色斑点或只出现痕迹的判为阴性，若A、B、C、D处有一个或一个以上出现较弱斑点，两周后需复查，其中，A液为样本稀释液，内为Tris-HCl缓冲液+5％Tween20，B液为洗涤液，内为Tris-HCl缓冲液+4％Tween20+9％NaCl，C液为金标抗人IgG抗体，内含胶体金、抗人IgG及明胶、庶糖，。When in use: First, lay the utility model flat, take 20 μl of serum or 40 μl of whole blood, put it in a centrifuge tube, and drop 5 drops of A solution (whole blood will be added after one minute), mix evenly (if the whole blood is turbid) Need 2000rpm centrifugation for 2 minutes), take 3 drops of the above-mentioned mixed liquid supernatant (or absorb 100 μ l), drop in the detection hole 3 of the present utility model; secondly, drop 3 drops of B liquid in the detection hole 3, drop 3 drops of C liquid In the detection hole 3, drop 3 drops of liquid B in the detection hole 3; then observe and record the results, usually in about 3 minutes to get the results, positive: "+" light red, "++" red, "++" +"Purple red, negative: "-"A, B, C, D without red spots or only traces are judged as negative, if one or more weak spots appear at A, B, C, D, two Re-examination is required after one week. Among them, liquid A is the sample diluent, which contains Tris-HCl buffer + 5% Tween20, liquid B is the washing liquid, which contains Tris-HCl buffer + 4% Tween20 + 9% NaCl, and liquid C It is a gold-labeled anti-human IgG antibody, which contains colloidal gold, anti-human IgG, gelatin, and sucrose.
为了便于吸收滴液，如附图2所示，纤维薄膜4与底座2之间有吸水纸5。In order to facilitate the absorption of drops, as shown in Figure 2, there is absorbent paper 5 between the fiber film 4 and the base 2.
如附图1所示，在纤维薄膜4上包被有阳性控制点E，而阳性控制点E为人体血清，若阳性控制点E处不显色，则说明该组合抗原包虫病快速诊断反应板已经失效而不能使用了。其中，上述纤维薄膜4可为醋酸纤维薄膜。As shown in accompanying drawing 1, the positive control point E is coated on the fiber film 4, and the positive control point E is human serum, if the positive control point E does not develop color, it means that the combined antigen echinococcosis rapid diagnostic response The board has failed and cannot be used. Wherein, the above-mentioned fiber film 4 may be a cellulose acetate film.
本实用新型结构简单、操作简便，应用于包虫病的早期诊断和鉴别诊断，使结果特异性强，灵敏度高，大大减少了漏诊率，一般仅需3分钟即查做出结果，故特别适用于样本的筛查。The utility model is simple in structure and easy to operate, and is applied to the early diagnosis and differential diagnosis of echinococcosis, so that the result has strong specificity and high sensitivity, and greatly reduces the rate of missed diagnosis. Generally, it only takes 3 minutes to check the result, so it is especially suitable screening of samples.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99219078.9U CN2395277Y (en) | 1999-08-09 | 1999-08-09 | Combination Antigen Echinococcosis Rapid Diagnosis Reaction Panel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99219078.9U CN2395277Y (en) | 1999-08-09 | 1999-08-09 | Combination Antigen Echinococcosis Rapid Diagnosis Reaction Panel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2395277Y true CN2395277Y (en) | 2000-09-06 |
Family
ID=34010100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99219078.9U Expired - Lifetime CN2395277Y (en) | 1999-08-09 | 1999-08-09 | Combination Antigen Echinococcosis Rapid Diagnosis Reaction Panel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2395277Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949926A (en) * | 2010-08-04 | 2011-01-19 | 浙江大学 | Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card |
| CN102608321A (en) * | 2012-02-29 | 2012-07-25 | 厦门大学 | Echinococcus multilocularis circulating antigen dot immunogold filtration kit and preparation method |
| CN102608330A (en) * | 2012-03-12 | 2012-07-25 | 中国疾病预防控制中心寄生虫病预防控制所 | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof |
| CN106092988A (en) * | 2016-06-08 | 2016-11-09 | 新疆医科大学第附属医院 | The method for quick of Echinococcus hydatid cyst protein molecular based on SOI self-luminous characteristic |
| CN107402298A (en) * | 2016-05-18 | 2017-11-28 | 上海新吉而生物科技有限公司 | A kind of dog excrement echinococcosis antigen quick detection kit and its application method |
| CN110261615A (en) * | 2019-07-04 | 2019-09-20 | 中国动物疫病预防控制中心(农业农村部屠宰技术中心) | Cystic echinococcosis diagnostic kit and its application |
-
1999
- 1999-08-09 CN CN99219078.9U patent/CN2395277Y/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949926A (en) * | 2010-08-04 | 2011-01-19 | 浙江大学 | Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card |
| CN102608321A (en) * | 2012-02-29 | 2012-07-25 | 厦门大学 | Echinococcus multilocularis circulating antigen dot immunogold filtration kit and preparation method |
| CN102608330A (en) * | 2012-03-12 | 2012-07-25 | 中国疾病预防控制中心寄生虫病预防控制所 | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof |
| CN107402298A (en) * | 2016-05-18 | 2017-11-28 | 上海新吉而生物科技有限公司 | A kind of dog excrement echinococcosis antigen quick detection kit and its application method |
| CN106092988A (en) * | 2016-06-08 | 2016-11-09 | 新疆医科大学第附属医院 | The method for quick of Echinococcus hydatid cyst protein molecular based on SOI self-luminous characteristic |
| CN106092988B (en) * | 2016-06-08 | 2019-03-01 | 新疆医科大学第一附属医院 | The rapid detection method of Echinococcus hydatid cyst protein molecular based on the spontaneous light characteristic of SOI |
| CN110261615A (en) * | 2019-07-04 | 2019-09-20 | 中国动物疫病预防控制中心(农业农村部屠宰技术中心) | Cystic echinococcosis diagnostic kit and its application |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |