CN201365914Y - Rapid diagnostic detection device - Google Patents
Rapid diagnostic detection device Download PDFInfo
- Publication number
- CN201365914Y CN201365914Y CNU2009201132816U CN200920113281U CN201365914Y CN 201365914 Y CN201365914 Y CN 201365914Y CN U2009201132816 U CNU2009201132816 U CN U2009201132816U CN 200920113281 U CN200920113281 U CN 200920113281U CN 201365914 Y CN201365914 Y CN 201365914Y
- Authority
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- China
- Prior art keywords
- checkout gear
- supporting zone
- region
- sample
- marked region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 239000000084 colloidal system Substances 0.000 claims description 9
- 238000003745 diagnosis Methods 0.000 claims description 9
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Images
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a rapid diagnostic detection device, which comprises a sample receiving area, a marking area, and a detecting area which is overlapped with the marking area, wherein the sample receiving area and the marking area are located on sample pads made of the same material; and the marking area contains colloidal gold particles or latex particles. The detection device also comprises a supporting area, wherein one end of the supporting area is connected with the marking area. The structure of the detection device is simpler than that of the traditional reagent strip, thereby greatly saving cost, meanwhile, the detection device is more convenient to assemble and install than the traditional reagent strip; furthermore, through a supporting structure overlapped on the marking area, the colloidal gold particles or the latex particles on the marking area are released more evenly, and the detection result with higher sensitivity can be obtained.
Description
Technical field
This utility model relates to a kind of quick diagnosis checkout gear, is mainly used in the quick diagnosis field, women's gestation particularly, drug abuse, the quick diagnosis of aspects such as infectious disease detection.
Background technology
Quick diagnosis checkout gear commonly used at present, especially quick diagnosis detectable bar, comprise the sample acceptance region, as shown in Figure 1, the assembly of forming reagent strip 1 comprises independently sample acceptance region 14, marked region 13, surveyed area 12 and absorption region 11, surveyed area 12 can be the nitrocellulose filter that comprises antigen-antibody, can the marks for treatment material on the marked region 13.This quick diagnosis checkout gear is at present more common.
Summary of the invention
This utility model relates to a kind of quick diagnosis checkout gear, comprise the sample acceptance region in turn, marked region and surveyed area, wherein, the one end overlap joint of marked region and surveyed area, sample acceptance region and marked region are positioned on the water absorption sample pad of identical material; Marked region contains colloid gold particle or latex particle.
Preferably, checkout gear also comprises supporting zone, and an end of this supporting zone is overlapped on the marked region.Wherein preferred, the overlap distance of supporting zone and marked region is 1 millimeter-4 millimeters.
Preferred in addition, an end in the whole supported zones of marked region overlaps.
Above-mentioned preferred, checkout gear comprises also and the absorption region of the other end overlap joint of surveyed area that the other end of supporting zone is overlapped on the absorption region.Particularly preferred, supporting zone is opaque non-water absorbing material.
More preferred, checkout gear also comprises a suction rod, this suction rod and sample acceptance region overlap joint.
In another preferred scheme, this utility model also comprises a housing, in this housing can be contained in sample pad and surveyed area.
Beneficial effect: this structure is more simple in structure than traditional reagent strip, has saved cost greatly, and is also easy to assembly than traditional reagent strip simultaneously; In addition,, make colloid gold particle or latex particle release ratio on the marked region more even, can obtain the testing result of higher sensitivity by being overlapped on the supporting construction on the marked region.
Description of drawings
Fig. 1 is the STRUCTURE DECOMPOSITION figure of detectable bar commonly used;
Fig. 2 is the structural representation of an embodiment of this utility model;
Fig. 3 is the structural representation of another embodiment of this utility model;
Fig. 4 is the structural representation of another embodiment of this utility model;
The detectable bar 1 that description of reference numerals: Fig. 1 represents, end card 10, absorption region 11, surveyed area 12, marked region 13, sample acceptance region 14.Reference numeral of the present utility model: detectable bar 100, absorption region 101, surveyed area 102, marked region 103, sample acceptance region 104, sample pad 105, suction rod 106, supporting zone 108, retaining muscle 308.
The specific embodiment
Structure or these employed technical terms to the liquid sample collection and detection device is described further below.
Detect the expression chemical examination or test a kind of material or whether material exists, such as, but be not limited to this, the metabolite of chemical substance, organic compound, inorganic compound, metabolism product, medicine or drug metabolite, organic organization or organic organization, nucleic acid, protein or polymer.In addition, detect the quantity of expression test substances or material.Furtherly, immune detection, chemical detection, enzyme detection etc. are also represented in chemical examination.
The sample of this utility model indication refers to that those can be used for detecting, chemically examining or diagnose the material that whether has analyte of interest matter.Sample can be that for example, liquid sample, liquid sample can comprise blood, blood plasma, serum, urine, saliva and various juice, can also comprise that solid sample and semi-solid samples are through anticipating the liquid solution that the back forms.The sample of collecting can be used for methods such as immune detection, chemical detection, enzyme detection and detect whether there is analyte.Checkout gear can be meant the detection in the immune field in this utility model, can also be chemical detection, also can also be that enzyme detects, and detects colloid gold label immune detection, ELISA detection etc. such as immunochromatography.
Preferably, sample acceptance region 104 and marked region 103 are positioned on the water absorption sample pad 105 of identical material, that is to say, comprise sample acceptance region 104 and marked region 103 on the sample pad 105.Marked region 103 and sample acceptance region 104 position on sample pad 105 can overlap each other, and also can be subregional.Preferably, an end of sample pad 105 is a marked region 103, and an other end is sample acceptance region 104.That is to say, comprise mark substance and sample acceptance region 104 on the sample pad 105 simultaneously, an end marks for treatment material of sample pad 105, the other end materials such as reaction needed buffer system or sample pretreatment fluid of finishing dealing with.This structure is more simple in structure than traditional reagent strip, has saved cost greatly, and is also easy to assembly than traditional reagent strip simultaneously.Forming the material of sample pad 105, can be absorptive material, as filter paper, and glass fibre, the material that polyester fiber or nitrocellulose filter etc. are commonly used.Preferably, sample pad 105 is made up of glass fibre.The glass fibre aperture is loose, relatively is fit to the transmission of liquid.Preferably, sample pad 105 is made up of the material of same integral body, and in concrete scheme, an end of sample pad 105 can be handled colloid gold particle or latex particle, and the other end of sample pad 105 can be handled the sample pretreatment fluid.The detectable bar 100 of this structure has been saved the material of assembling detectable bar 100 relatively, and do not influence detectable bar 100 be used for detecting the function whether sample contains analyte.
On sample acceptance region 104, comprise and finish reaction necessary material, for example some buffer reagents, pretreatment sample reagent or the like.The main purpose of sample acceptance region 104 is the smooth absorption fluids samples that get, comprise liquid sample and semiliquid sample, such as blood, blood plasma, serum, urine, saliva and various juice, can also comprise that solid sample and semi-solid samples are through anticipating the liquid solution that the back forms.
Can comprise the mark substance that is used for detection reaction on the marked region 103, by soaking, modes such as dropping are handled on marked region 103.Such as colloid gold particle or latex particle, and the common complex of forming of antigen-antibody.The immune labeled material of this solubility can comprise the fluorescent labeling material on marked region 103, the colloid gold label granule, or with painted gel marking particle, preferably can also be the water solublity mark substance.These mark substances can connect antibody, antigen, hapten or analyte similar substance or the like.This label is handled after marked region 103 or sample pad 105, can be through the oven dry supervisor, make it become dry, when running into liquid sample, this dry can be dissolved in rapidly in the liquid sample, and antigen or the analyte in antibody and the liquid sample or other related substances combinations by being attached thereto, can on surveyed area 102, embody testing result at last, and show in the sample whether contain analyte with macroscopic lines directly perceived.Two zones are set on same sample pad 105, can on production technology, save cost greatly, in addition sensitivity and also not influence of specificity detecting.
Checkout gear can also comprise a supporting zone 108, and an end of this supporting zone 108 is overlapped on the marked region 103.Overlap joint is meant that two different zones jointly by the method for intermeshing, make two zones can be in the state of adhesion, and in laterally flow measurement detected, this overlap joint can make between two zones and be in the liquid communication state.The marked region 103 that overlaps with supporting zone 108 has been protected in the setting of this supporting zone 108 to a certain extent, makes the raw material on the marked region 103 can follow sample even must flowing on detectable bar 100.Preferably, an end of supporting zone 108 is overlapped on the marked region 103, and overlap distance can be between 1 millimeter-4 millimeters.Overlap distance is meant eclipsed part between two zones, and as shown in Figure 2, d can represent the overlap distance between supporting zone 108 and the marked region 103; Overlap distance, it can also be eclipsed part between other zones, there is overlap distance such as the overlap joint between surveyed area 102 and the absorption region 101, the overlap distance that overlap joint between surveyed area 102 and the supporting zone 108 exists, the overlap distance that overlap joint between marked region 103 and the surveyed area 102 exists, overlap distance that the overlap joint between suction rod 106 and the sample acceptance region 104 exists or the like.More preferred, its overlap distance is 0.5mm, 1mm, 2mm, 3mm, 4mm or other distance.Raw material on the marked region 103; behind supporting zone 108 overlap joints; sample flow is when marked region 103; because the compacting of supporting zone 108; liquid liquid velocity in flow through marked region 103 and supporting zone 108 lap-joints is reduced; like this, colloid gold particle on the marked region 103 or latex particle just can have sufficient time and other materials fully to react, so that improve the sensitivity that detects.Preferred in addition, marked region 103 whole supported regional 108 overlap joints that is to say that an end of marked region 103 supported regional 108 covers.The overlap joint of this supporting zone, this advantage of the response time of the raw material on can prolonging marked region 103, can also make detectable bar 100 structurally more firm, as be in high temperature or other environment flatness that keeps detectable bar 100, thereby the length of the overlap distance between supporting zone 108 and the marked region 103, can have influence on detectable bar 100 structural soundness, overlap distance is long more, and detectable bar 100 is firm more.In addition, supporting zone 108 after covering marked region 103 fully, and then with sample acceptance region overlap joint, the overlap joint of this mode also is feasible.
Above-mentioned preferred, checkout gear can also comprise and the absorption region 101 of the other end overlap joint of surveyed area 102 that the other end of supporting zone 108 is overlapped on the absorption region 101.This mode, an end that can form supporting zone 108 is overlapped on the marked region 103, and the other end is overlapped on the structure on the absorption region 101.This structure can allow on the one hand the release of the mark substance on the marked region 103 controlled, on the other hand, can allow the structure of detectable bar 100 more firm, and the supporting role of supporting zone 108 is more obvious.Its reason is, the material of surveyed area 102 is made up of nitrocellulose filter usually, just usually said NC film, and this membranous ground is soft, is not having under the situation of external support, and detectable bar 100 may be in case of bending, is unfavorable for the carrying out that detects; And by supporting zone 108, can be in this respect the structure of detectable bar be optimized.The structure that the major function of supporting zone 108 is optimized the detectable bar reaches the purpose of improving detection efficiency.
The material of forming supporting zone 108, preferred non-absorptive material.Non-absorptive material can be transparent, also can be opaque.Transparent non-water absorbing material can be by supporting zone 108 testing results that must read on the surveyed area 102 directly perceived; When selecting supporting zone 108 that opaque non-water absorbing material forms, positive supported regional 108 of the surveyed area 102 parallel with supporting zone 108 stop, can by the back side of surveyed area 102 read the result of detection.Non-water absorbing material does not deform so that keep the structure of detectable bar 100 owing to can not kept its original shape by liquid-soaked.Wherein preferred, the material of supporting zone 108 can be plastics.Plastics are industrial materials relatively more commonly used, and it is cheap, and wide material sources are comparatively suitable materials.Wherein, selecting the material of polyester sheet as supporting zone 108, is better.Polyester sheet has better hardness, and liquid can flow through from polyester sheet but can not soak into polyester sheet itself, can play supporting role preferably in detectable bar 100.
This utility model can also comprise the housing that holds detectable bar 100.This housing can be that flexible material is formed, and also can be that hard material constitutes.In a specific embodiment; housing is made up of adhesive sticker; this adhesive sticker will wrap up or plastic packaging around the detectable bar 100, makes it keep the Stability Analysis of Structures of detectable bar 100 when using separately, also protects not moisture-sensitive of detectable bar 100 on the other hand.In another specific embodiment, in detectable bar 100 can be wrapped in by the material of hard, can have the window that window that testing result reads and sample drip on this housing.Similar housing is laid-open U.S. Patents US60520437 formerly, US6565808, US6890484, US7270959 and Chinese patent ZL200730116163.7, ZL20062010602.5, ZL200620140828.8, ZL200620140826.9, ZL200820083730.2, ZL200720114251.8, ZL20063011543.7 can find among the ZL200730113362.2, the detectable bar 100 that these previously described housings can be described with this utility model in conjunction with or be assembled into an independently product.Usually, detectable bar 100 is positioned in housing, makes it when detection makes and read testing result, is in firm state.Localized mode, can be undertaken by the cooperation between the rivet of enclosure interior and the corresponding with it hole and the screens effect of draw-in groove, can be divided into upper plate and lower plate as housing, the draw-in groove that identical length and width are set all around with detectable bar 100 is set on lower plate, detectable bar 100 is placed in one, at four angles of upper plate 4 rivets are set then, four angles of lower plate are provided with the hole, mating reaction by rivet and hole cooperates the upper plate and the lower plate of housing, and detectable bar 100 is fixed in the housing.
In order to increase combining closely of supporting zone 108 and marked region 103 or absorption region, more preferred, the retaining muscle 308 of some projections is set, to prevent " mighty torrent " phenomenon on the position corresponding to marked region 108 or absorption region 101.The retaining muscle 308 of this projection can increase the bonding tightness of supporting zone 108 and marked region 103 by compressing supporting zone 108, thereby allows the mark substance on the marked region 103 discharge more even.More preferred, the retaining muscle 308 of projection is located immediately at the place of supporting zone 108 and marked region 103 overlap joints, increases both bonding tightness by the supporting zone 108 on the direct pressure marked region 103.As shown in Figure 4.Better, the retaining muscle 308 of projection is set simultaneously in the place of the place that is positioned at supporting zone 108 and marked region 103 overlap joints and supporting zone 108 and absorption region 101 overlap joints, effect is better like this.Certainly, the retaining muscle 308 of this projection can be set directly at the last intralaminar part of housing.The advantage of supporting zone 108 in the checkout gear of this boxlike that housing arranged is more obvious, can prevent mark substance on the one hand because the compacting of compacting, the especially draw-in groove of housing and warping has overcome mark substance and discharged uneven shortcoming; On the other hand, can make detectable bar 100 be positioned at more smooth state, be unlikely to because the influence of temperature or humidity is crooked or distortion; The 3rd, under the too much situation of sample, the checkout gear of this structure can slow down " mighty torrent " phenomenon.
Preferably, this utility model can also comprise the suction rod 106 that can directly absorb liquid from sample except comprising the housing that can hold detectable bar 100.Suction rod 106 structurally overlaps with sample acceptance region 101.Having under the situation of housing, excellent 106 parts that absorb water are in outside the housing, be used for contacting with extraneous liquid sample, a part be within the housing and detectable bar 100 on sample acceptance region 101 overlap joint.Sample arrives sample acceptance region 101 by suction rod 106, carries out the sample pretreatment on sample acceptance region 101, then just with the reacting substance association reaction to show in the sample whether contain analyte.
Below in conjunction with concrete accompanying drawing examples of implementation of the present utility model are described in detail.These specific embodiment only are limited the enumerating under this utility model spirit, do not get rid of one of ordinary skill in the art prior art and this utility model in conjunction with and other specific embodiments of producing.
Embodiment 1:
Whether conceived present embodiment be one and detect women's detectable bar 100, as shown in Figure 2, comprises the sample pad 105 that is made of glass, and by the detecting pad 102 that nitrocellulose filter constitutes, filter paper constitutes the supporting pad 108 that absorption pad 101 and polyester sheet constitute.Wherein comprise sample acceptance region 104 and marked region 103 on the sample pad 105.Handled buffer substances such as tr i s on the sample acceptance region 104, be used for the pretreatment of sample, as the filtration of impurity in the sample etc.On marked region 103, handling has colloid gold particle and the bonded polyclonal antibody of specificity, on surveyed area 102, handle have can with the bonded polyclonal antibody of the specificity on the marked region 103.The detectable bar 100 that assembling finishes can use separately.Concrete using method is: the part of sample acceptance region 104 is immersed in the liquid sample of collecting, took away later in 1 minute, perhaps splash into 3-5 and drip sample in sample acceptance region 104, horizontal detectable bar 100, read testing result at surveyed area 102 after 3 minutes, the C line is wired on the surveyed area 102, and the T line does not have line, represents that this tester does not have pregnancy; The C line is wired, and the T line is wired, represents this tester's pregnancy; The C line does not have line, and no matter whether the T line has line, represents that this result is invalid, need carry out other test.
Embodiment 2:
What present embodiment and embodiment 1 were different is also to comprise a housing in the present embodiment, in detectable bar 100 can being contained in.On housing, include window that can read testing result and the hole that can add sample.
Embodiment 3:
What present embodiment and embodiment 1 were different is, also comprise in the present embodiment one can be directly from the suction rod 106 of extraneous imbitition.Sample receiving pad 104 overlap joints of this suction rod 106 and sample pad 105.
Embodiment 4:
Different with embodiment 3 is, also includes a housing in the present embodiment, and this housing can hold detectable bar 100.On housing, also have the window that can read testing result.In addition, the part of suction rod 106 is positioned at enclosure interior, and a part is exposed housing in addition, the part of exposing can be from sample imbitition.
Claims (12)
1. a quick diagnosis checkout gear comprises the sample acceptance region in turn, marked region, and surveyed area, wherein, an end of marked region and surveyed area overlap joint; It is characterized in that sample acceptance region and marked region are positioned on the water absorption sample pad of identical material; Marked region contains colloid gold particle or latex particle.
2. checkout gear as claimed in claim 1 is characterized in that described checkout gear also comprises supporting zone, and an end of supporting zone is overlapped on the marked region.
3. checkout gear as claimed in claim 2 is characterized in that, the overlap distance of described supporting zone and marked region is 1 millimeter-4 millimeters.
4. checkout gear as claimed in claim 2 is characterized in that, an end in the whole supported zones of described marked region overlaps.
5. as the described checkout gear of one of claim 2-4, it is characterized in that described checkout gear comprises also and the absorption region of the other end of surveyed area overlap joint that the other end of described supporting zone is overlapped on the absorption region.
6. as the described checkout gear of one of claim 2-4, it is characterized in that described supporting zone is opaque non-water absorbing material.
7. as the described checkout gear of one of claim 2-4, it is characterized in that described supporting zone is a polyester sheet.
8. as the described checkout gear of one of claim 1-4, it is characterized in that the material of forming sample pad is selected from glass fibre, a kind of in polyester fiber or the filter paper.
9. as the described checkout gear of one of claim 1-4, it is characterized in that described surveyed area comprises a nitrocellulose filter pad, handling on this pad has one deck antibody or antibody fragment.
10. as the described checkout gear of one of claim 2-4, it is characterized in that described checkout gear comprises that also one increases the retaining muscle that supporting zone and marked region are combined closely by the compressing supporting zone.
11., it is characterized in that described checkout gear also comprises a housing as the described checkout gear of one of claim 2-4, also being provided with one on this housing increases the retaining muscle that supporting zone and marked region are combined closely by the compressing supporting zone.
12. as the described checkout gear of one of claim 2-4, it is characterized in that, described checkout gear also comprises the absorption region with the other end of surveyed area overlap joint, the other end of described supporting zone is overlapped on the absorption region, simultaneously, be provided with one simultaneously at the two ends of supporting zone and increase supporting zone and marked region tightly and the absorption region retaining muscle of combining closely by the compressing supporting zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2009201132816U CN201365914Y (en) | 2009-02-05 | 2009-02-05 | Rapid diagnostic detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2009201132816U CN201365914Y (en) | 2009-02-05 | 2009-02-05 | Rapid diagnostic detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201365914Y true CN201365914Y (en) | 2009-12-23 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2009201132816U Expired - Lifetime CN201365914Y (en) | 2009-02-05 | 2009-02-05 | Rapid diagnostic detection device |
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| CN (1) | CN201365914Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102169120A (en) * | 2010-02-25 | 2011-08-31 | 艾博生物医药(杭州)有限公司 | Detector |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
-
2009
- 2009-02-05 CN CNU2009201132816U patent/CN201365914Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102169120A (en) * | 2010-02-25 | 2011-08-31 | 艾博生物医药(杭州)有限公司 | Detector |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20091223 |