CN223516962U - Automated filtration and high-pressure separation and purification equipment, automated separation and purification preparation system - Google Patents

Abstract

The present disclosure provides an automated filtration and high pressure separation and purification apparatus and an automated separation and purification preparation system. The automatic filtering and high-pressure separating and purifying device comprises a workbench, a first filtering module, a second filtering module, a high-pressure sample inlet and outlet module, a pump valve analysis module and a carrying mechanism which are arranged on the workbench, a mobile phase buffer module which is arranged independently of the workbench, a high-pressure sample inlet and outlet module, a carrying mechanism, a mobile phase buffer module and a pump valve analysis module, wherein the first filtering module and the second filtering module are used for filtering sample liquid according to first filtering requirements and second filtering requirements respectively, the high-pressure sample inlet and outlet module is used for introducing the filtered sample liquid to collect fractions, the carrying mechanism is used for enabling products after the sample liquid and the fractions are collected to automatically enter and exit, the carrying mechanism is used for transferring materials in the first filtering module, the second filtering module and the high-pressure sample inlet and outlet module, the mobile phase buffer module is used for buffering and placing mobile phases, and the pump valve analysis module is connected with the high-pressure sample inlet and outlet module and the mobile phase buffer module for carrying out pump liquid and analysis treatment. The present disclosure enables full flow automation of filtration and high pressure processing.

Description

Automatic filtration and high-pressure separation and purification equipment and automatic separation and purification preparation system

Technical Field

The disclosure relates to the technical field of automation equipment, in particular to the technical field of medicine and chemical automation equipment, and especially relates to an automatic filtration and high-pressure separation and purification equipment and an automatic separation and purification preparation system.

Background

Experiments in biology, medicine, chemistry and other industries often involve a large number of steps such as filtration, component separation, pipetting, split charging and the like, at present, each step is mainly manually operated, and a large number of operators are needed to participate in the steps in the treatment process, so that the treatment efficiency is low, the error is large, and the operators are required to directly contact possibly harmful chemicals or biological samples, so that the problem of potential health risks exists.

Disclosure of utility model

In view of the above problems, the present disclosure provides an automatic filtration and high-pressure separation and purification device, an automatic separation and purification preparation system, which can realize the steps of full-automatic filtration, separation, pipetting, sub-packaging, etc., greatly improve the processing speed of samples, reduce human errors, and improve the accuracy and safety of experiments.

In a first aspect, an embodiment of the disclosure provides an automated filtration and high-pressure separation and purification device, which comprises a workbench, a first filtration module, a second filtration module, a high-pressure sample preparation and inlet module, a pump valve analysis module and a carrying mechanism which are arranged on the workbench, and a mobile phase buffer module which is arranged independently of the workbench, wherein the first filtration module filters sample liquid according to a first filtration requirement, the second filtration module filters the sample liquid according to a second filtration requirement, the high-pressure sample preparation and inlet module introduces the sample liquid filtered by the first filtration module or the second filtration module for fraction collection, the carrying mechanism enables the sample liquid and a product after fraction collection to automatically enter and enter the workbench, the carrying mechanism is used for transferring materials in the first filtration module, the second filtration module and the high-pressure sample preparation and inlet module, the mobile phase buffer module is used for buffering and placing mobile phases, and the pump valve analysis module is respectively connected with the high-pressure sample preparation module and the mobile phase buffer module for processing of pump liquid and analysis.

In some embodiments, the automated filtration and high pressure separation and purification apparatus further comprises an interaction unit for material interaction with the outside through a handling mechanism, and a pipetting module for transferring the fraction collected sample fluid to a predetermined container according to a predetermined pipetting capacity.

In some embodiments, the pump valve analysis module introduces the sample liquid from the high-pressure sample inlet and outlet module, introduces the mobile phase corresponding to the sample liquid from the mobile phase buffer module, performs component separation and analysis on the mixed liquid of the sample liquid and the mobile phase, and then leads out different components to the high-pressure sample inlet and outlet module.

In some embodiments, the automated filtration and high pressure separation and purification apparatus further comprises a housing rack for holding materials required for processing by each module, the interaction unit, the housing rack, the first filtration module, the second filtration module, the high pressure sample inlet and outlet module, and the pipetting module are disposed around the handling mechanism within an operable range of the handling mechanism.

In some embodiments, the mobile phase caching module includes a plurality of mobile phase caching boxes in which the bulk tanks containing mobile phases are placed.

In some embodiments, the workbench comprises a front side, a rear side, a left side and a right side, the interaction unit is arranged on the front side, the storage rack is arranged on the left side, the first filter module, the second filter module and the pipetting module are arranged on the rear side in parallel, the high-pressure sample inlet and outlet module is arranged between the front side and the rear side at a position closer to the front side, and the pump valve analysis module is arranged at a position adjacent to the high-pressure sample inlet and outlet module and far away from the conveying mechanism.

In some embodiments, the first filtration module is a positive pressure filtration module for filtering small amounts of sample fluid and the second filtration module is a screen filtration module for filtering large amounts of sample fluid.

In some embodiments, the positive pressure filtration module comprises a clamping mechanism for clamping a filtration vessel containing a sample to be filtered, an air inlet connector positioned above the clamping mechanism and connected with an air source for communicating the air source with the sample to be filtered during filtration, a first lifting mechanism connected with the air inlet connector and used for driving the air inlet connector to lift, a second lifting mechanism, wherein the clamping mechanism and the first lifting mechanism are fixed on the second lifting mechanism, the second lifting mechanism is used for driving the clamping mechanism, the air inlet connector and the first lifting mechanism to lift together, a removing mechanism for removing a plug below the filtration vessel, and a receiving bottle placement mechanism for placing a receiving bottle for receiving filtered sample liquid.

In some embodiments, the positive pressure filtration module further comprises a base, a pulling mechanism and a receiving bottle placement mechanism are arranged on the base, a bracket is vertically arranged on the base, a first supporting plate and a second supporting plate are arranged on the bracket in a vertically crossed mode, the second supporting plate is fixed on the first supporting plate and can move along the first supporting plate, and the second lifting mechanism is fixedly connected on the second supporting plate.

In some embodiments, the filter screen filter module comprises a vertically arranged bracket, a receiving bottle positioning frame, a filter assembly supporting frame, a lifting mechanism and a sealing pressing plate, wherein the receiving bottle positioning frame is arranged at the bottom of the bracket and used for placing a receiving bottle for receiving filtered sample liquid, the filter assembly supporting frame is arranged above the receiving bottle positioning frame and fixed on the bracket and used for placing a filter assembly with a filter screen, the lifting mechanism is arranged on the bracket, the sealing pressing plate is connected with the lifting mechanism and drives the sealing pressing plate to lift, and an air inlet connector is arranged on the sealing pressing plate and connected with an air source and used for communicating the air source with the filter assembly during filtration.

In some embodiments, the lifting mechanism comprises a lifting assembly, a linear guide rail and a motor, wherein the linear guide rail is fixed at the upper position of the bracket, the lifting assembly is connected with the sealing pressing plate, and the lifting assembly moves up and down along the linear guide rail through driving of the motor.

In some embodiments, the high-pressure preparation sample inlet and outlet module comprises a fraction collecting unit, a fraction collecting drawing unit and a fraction collecting lifting unit, wherein the fraction collecting unit comprises a sample inlet needle and a sample outlet needle and is used for collecting fractions of imported sample liquid, the fraction collecting drawing unit comprises a sample inlet position corresponding to the sample inlet needle, a fraction collecting position corresponding to the sample outlet needle and a component capable of pulling or pushing the sample inlet position and the fraction collecting position out of the fraction collecting unit, and the fraction collecting lifting unit is used for lifting the fraction collecting position.

In some embodiments, the sample injection needle of the fraction collecting unit is configured to be liftable, the sample injection position of the fraction collecting and drawing unit is configured to be stationary, and the sample discharge needle of the fraction collecting unit is configured to be stationary, and the fraction collecting position of the fraction collecting and drawing unit is configured to be liftable.

In some embodiments, the fraction collecting and pulling unit comprises a sample introduction position for placing a sample to be processed, a fraction collecting position for collecting fractions, a first sliding rail connected with the sample introduction position, a bottom plate, a plurality of second sliding rails fixed on the bottom plate, wherein the second sliding rails are arranged corresponding to the fraction collecting position, the fraction collecting position can move along the second sliding rails, and a pulling plate is arranged at the tail end of the fraction collecting position, and the fraction collecting position can move along the second sliding rails by applying force to the pulling plate.

In some embodiments, a limiting block is arranged on the second sliding rail, a limiting groove is arranged on the fraction collecting position, and when the fraction collecting position is pulled out, the fraction collecting position is fixed on the second sliding rail through the clamping of the limiting block and the limiting groove.

In some embodiments, the fraction collecting lifting unit is arranged below the bottom plate and comprises a driving motor and a lifting rod, the lifting rod is connected to the bottom plate, the driving motor drives the lifting rod to lift, a protruding piece and a clamping hole are respectively arranged on the fraction collecting position and the sample feeding position, when the fraction collecting position is lifted, the protruding piece is separated from the clamping hole, the fraction collecting position is separated from the sample feeding position, and when the fraction collecting position is lowered, the protruding piece is inserted into the clamping hole, and the fraction collecting position is connected with the sample feeding position.

In some embodiments, the high pressure preparation sample entry and exit module includes a set of sample entry sites and three sets of fraction collection sites.

In some embodiments, the pipetting module comprises a support comprising a support riser and a support cross plate connected, a pipetting assembly arranged on the support riser and used for being detachably connected with the pipette tip, the pipetting assembly further used for driving the pipette tip to aspirate or spit liquid, and a head withdrawing assembly arranged on the support cross plate and used for pushing the pipette tip to be separated from the pipetting assembly.

In some embodiments, the interaction unit comprises a bottom plate and a supporting plate, wherein the bottom plate is arranged on the workbench, the supporting plate is connected to two ends of the bottom plate and is vertically arranged, the placing plate is connected between the supporting plates and is used for storing a material container and/or a tray, a plurality of first bin positions are arranged on the placing plate, each first bin position comprises a positioning piece and a sensor, the positioning piece is used for limiting the material container and/or the tray, and the sensor is used for detecting whether the bin positions store the material container and/or the tray.

In some embodiments, the handling mechanism includes a handling arm and a handling tool, the handling arm is a mechanical arm or a moving mechanism having at least one degree of freedom in one direction, one end of the handling arm is fixed to the table, the handling tool is mounted at the other end of the handling arm, and the handling tool is detachably connected with the handling arm.

In some embodiments, the handling arm is any one of a four-axis mechanical arm, a six-axis mechanical arm, and an XYZ three-axis linear motion mechanism, and the handling tool is a container jaw or a tray jaw.

In some embodiments, a tool magazine is further provided on the table for storing a plurality of handling tools, and the handling mechanism takes and places the handling tools from the tool magazine.

In some embodiments, the storage rack comprises at least one deck of storage panels and two vertically disposed supports, the storage panels being disposed between the two supports, the storage panels being provided with a second bin for holding containers and/or trays.

In some embodiments, a cover is further provided on the workbench, an internal accommodating space is formed between the cover and the workbench, an interaction window is provided on the cover at one side where the interaction unit is provided for material interaction with the external space, and maintenance windows are provided on the cover at the other sides of the cover for maintenance of the modules of the internal accommodating space.

In some embodiments, the interactive window is configured as a sliding window and the maintenance window is configured as a flat-open window.

In a second aspect, embodiments of the present disclosure provide an automated separation and purification preparation system comprising a mobile device and the automated filtration and high pressure separation and purification device of the first aspect, the mobile device configured to deliver material to the automated filtration and high pressure separation and purification device and to remove samples processed by the automated filtration and high pressure separation and purification device.

The automatic filtering and high-pressure separating and purifying equipment and the automatic separating and purifying preparation system can select a proper filtering mode according to the amount of the sample to be filtered, the whole treatment process is realized automatically, the contact between the samples can be reduced, and the risk of cross contamination is reduced. In addition, operators do not need to directly contact potentially harmful chemicals or biological samples, potential health risks are reduced, and the automated system can accurately control the usage amount of reagents, reducing waste, especially when expensive reagents are used.

Drawings

FIG. 1 is a top view of an automated filtration and high pressure separation and purification apparatus of an embodiment of the present disclosure with a cover removed;

FIG. 2 is a schematic perspective view of an automated filtration and high pressure separation and purification apparatus according to one embodiment of the present disclosure with the housing removed;

FIG. 3 is a perspective view of a positive pressure filtration module according to an embodiment of the present disclosure;

FIG. 4 is an enlarged partial view of a positive pressure filtration module according to an embodiment of the present disclosure;

FIG. 5 is a perspective view of a screen filtration module according to an embodiment of the present disclosure;

FIG. 6 is a rear view of a screen filtration module according to an embodiment of the present disclosure;

FIG. 7 is a perspective view of a high pressure sample access module according to an embodiment of the present disclosure;

FIG. 8 is a perspective view of a drawer assembly and a lift assembly according to an embodiment of the present disclosure;

FIG. 9 is a side view of a drawer assembly and a lift assembly according to an embodiment of the present disclosure;

FIG. 10 is a perspective view of a pipetting module of an embodiment of the disclosure;

FIG. 11 is a perspective view of one mobile phase cache box in a mobile phase cache module according to one embodiment of the present disclosure;

FIG. 12 is a perspective view from the front of an automated filtration and high pressure separation and purification apparatus with a housing according to an embodiment of the present disclosure;

Fig. 13 is a perspective view from the back side of an automated filtration and high pressure separation and purification apparatus according to an embodiment of the present disclosure with a housing.

Detailed Description

In order to better understand the technical solutions of the present disclosure, the following detailed description of the technical solutions of the present disclosure is provided with reference to the accompanying drawings.

Example embodiments will be described more fully hereinafter with reference to the accompanying drawings, but may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

Embodiments of the disclosure and features of embodiments may be combined with each other without conflict.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure. As used herein, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

Embodiments described herein may be described with reference to plan and/or cross-sectional views with the aid of idealized schematic diagrams of the present disclosure. Accordingly, the example illustrations may be modified in accordance with manufacturing techniques and/or tolerances. Thus, the embodiments are not limited to the embodiments shown in the drawings, but include modifications of the configuration formed based on the manufacturing process. Thus, the regions illustrated in the figures have schematic properties and the shapes of the regions illustrated in the figures illustrate the particular shapes of the regions of the elements, but are not intended to be limiting.

Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and the present disclosure, and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Some embodiments of the present disclosure are described in detail below with reference to the attached drawings. Referring to fig. 1 and 2, fig. 1 is a top view of an automated filtration and high pressure separation and purification apparatus of one embodiment of the present disclosure, and fig. 2 is a schematic perspective view of the automated filtration and high pressure separation and purification apparatus of one embodiment of the present disclosure. The automated filtration and high pressure separation and purification apparatus 100 includes a table 11, and a plurality of process modules, which will be described in detail below, are provided on the table 11. The table 11 includes a first side, front side 111, a second side, rear side 112, a third side, left side 113, and a fourth side, right side 114. The front side 111 refers to a side that is close to the user in a natural use state. The rear side 112 is the side opposite to the front side 111 and refers to the side away from the user in use. The left side 113 refers to the left side of the user in the use state. The right side 114 is the side opposite to the left side 111, and refers to the right side of the user in the use state.

The interactive unit 1 is disposed near a first side, i.e., a front side 111, of the table 11 and near a third side, i.e., a left side 113, for interacting with external materials. For example, the material containers and/or trays transported by the mobile device, such as an external AGV cart, are stored, and the sample containers and/or trays processed in the system are placed in the interaction unit 1 and transported by the mobile device to other processing systems.

In some embodiments, the sample is a biological, pharmaceutical, or chemical sample. When the sample is a medical sample, the sample can be a western medicine sample, a traditional Chinese medicine sample, or other medicine samples with medicine effect, etc. For example, the Chinese medicinal sample comprises Chinese medicinal materials, chinese medicinal decoction pieces, chinese patent medicines, chinese medicinal semi-finished products, medicinal plants, etc.

The interactive unit 1 comprises a base plate 105 arranged on the table 11, support plates 104 connected to opposite ends of the base plate 105 and arranged vertically, a placement plate 103 connected to the support plates 104, the placement plate 103 being used for storing material containers and/or trays. The placement plate 103 is provided with at least two first compartments for storing material containers and/or trays, each first compartment comprising a set of positioning members 101 and sensors 102. The positioning pieces 101 can be provided with two structures such as bulges or grooves, and the corresponding structures at the bottoms of the material containers and/or the trays can be clamped with the bulges or the grooves, so that the positioning pieces 101 can limit the positions of the material containers and/or the trays. The positioning piece 101 and the sensor 102 are connected to the placing plate 103, the positioning piece 101 is used for limiting the material container and/or the tray to the bin, and the sensor 102 is used for sensing whether the bin stores the material container and/or the tray. Alternatively, multiple layers of placing plates 103 may be disposed at intervals, and at least two first spaces are disposed on each layer of placing plates 103. By means of the arrangement, the interaction unit 1 is provided with a plurality of first bins, and the bins can be used for simultaneously taking and placing the material containers and/or the trays, so that efficiency can be improved.

The conveyance mechanism 20 is disposed at the center of the table 11 closer to the second side, i.e., the rear side 112, and closer to the third side, i.e., the left side 113. The handling mechanism 20 is used to transfer material between the process modules, and therefore, the handling mechanism 20 is positioned in a location that requires sufficient space for movement and that facilitates interaction with the process modules to which material is to be transferred. The position where the conveying mechanism 20 is provided is not particularly limited as long as the above condition is satisfied.

Alternatively, the conveyance mechanism 20 includes a conveyance arm 3 and a conveyance tool 4 mounted at one end of the conveyance arm 3. The handling arm 3 may be a mechanical arm or a moving mechanism having at least one degree of freedom in one direction, and specifically, the handling arm 3 may be a multi-degree-of-freedom mechanical arm, for example, a four-axis mechanical arm, a six-axis mechanical arm, or the like, or may be an XYZ three-axis linear moving mechanism. One end of the carrying arm 3 is fixed on the workbench 11, and the carrying tool 4 is mounted on the other end, and the carrying tool 4 is detachably connected with the carrying arm 3. The carrier 4 may be a motorized jaw, which switches different carriers 4, e.g. container jaws, tray jaws, etc., corresponding to the pallet or container to be transferred. A tool magazine is provided near the position where the carrying mechanism 20 is provided, and a plurality of kinds of carrying tools 4 are stored in the tool magazine. Optionally, in an embodiment, the handling arm 3 is provided with a quick-change male head, and each handling tool 4 is provided with a quick-change female head, and the quick-change male head cooperates with the quick-change female head to realize detachable connection of the handling arm 3 and the handling tool 4. The quick-change male head and the quick-change female head can be connected in a pneumatic connection, magnetic connection and other modes to realize quick connection, and the quick-change male head and the quick-change female head are not limited herein. The carrying arm 3 drives the carrying tool 4 to move, clamps the corresponding container or tray, and transfers the container or tray to the target position.

Optionally, at least one storage rack 2 may be further disposed on the working table 11. In the embodiment shown in fig. 1 and 2, two storage racks 2 are provided on the table 11. One of the storage racks 2 is disposed at a position close to the third side edge, i.e., the left side edge 113, between the left side edge 113 and the carrying mechanism 20, and the other storage rack 2 is disposed between the carrying mechanism 20 and the high-pressure sample inlet and outlet module 5. When the mobile device has more material containers and/or trays, the interactive unit 1 can not be accommodated at one time, the material containers and/or trays can be temporarily stored on the storage rack 2. For example, in the preparation stage of the experiment, the mobile device transfers various materials required for the experiment, such as empty test tube trays, filter head trays, tip head trays, empty liquid phase vial trays, etc., to the interaction unit 1, and the handling mechanism 20 transfers various trays on the interaction unit 1 to the storage rack 2. In the course of the experiment, samples processed in the filtration module, the high-pressure sample inlet/outlet module, and the like may be temporarily stored in the storage rack 2, and then transferred to the next processing module by the transport mechanism 20. After all the processing of the samples is completed, the containers and/or trays on the storage rack 2 are transferred to the interaction unit 1, and are conveyed to other processing systems by the mobile equipment. The housing rack 2 is used for placing trays, containers, etc., including but not limited to test tubes, solvent bottles, etc. Referring to fig. 2, the storage rack 2 includes at least one storage plate 202 and two opposite vertically disposed supports 201, the storage plate 202 being disposed between the two supports 201. The storage plate 202 is provided with a second compartment for receiving containers and/or trays. The second bin is provided with a securing member 203 for securing the container and/or tray to the storage plate 202. The storage boards 202 may be provided in multiple layers to store different amounts and/or different types of materials according to different needs. For example one layer for placing containers and one layer for placing trays.

Optionally, the automatic filtration and high-pressure separation and purification apparatus 100 further includes a detection assembly, which may be disposed on the handling mechanism 20, for detecting whether there are material containers and/or trays on the interaction unit 1 or the storage rack 2, and detecting materials contained in the material containers and/or trays. The detection assembly may include a variety of detection members, identification members, etc., without limitation. The detection assembly can detect and identify the material container and/or tray, the material and the like through any feasible detection principle, identification principle and the like so as to facilitate the material transfer between the modules and the material treatment management in the modules.

Optionally, the automated filtration and high pressure separation and purification apparatus 100 further comprises a control system electrically connected to the detection assembly, the control system configured to receive and manage information detected by the detection assembly. The control system is a control background of the automatic filtering and high-pressure separation and purification equipment 100, and can manage the material operation of the automatic filtering and high-pressure separation and purification equipment 100. The control system may be composed of hardware such as a computer, and suitable software, and the embodiment of the present application is not limited thereto. The electrical connection between the control system and the detection assembly may be a wired or wireless connection, and is not limited herein. The information detected by the detection component is input to the control system, the control system can process the information and obtain information such as the running state and the running position of the material, and the work flow of the automatic filtering and high-pressure separation and purification device 100 is scheduled according to the processing requirement, and the embodiment of the application is not limited.

Referring to fig. 1 and 2, the first filter module 7 and the second filter module 8 are juxtaposed near a second, rear side 112 of the table 11 and are proximate to the conveyance mechanism 20. The first filtering module 7 and the second filtering module 8 are used for filtering sample liquid, and the filtered sample liquid is used for high-pressure preparation, sample inlet and outlet module 5 or is conveyed to other working procedures by mobile equipment. In an embodiment, the first filter module 7 and the second filter module 8 correspond to different filter requirements. The first filter module 7 is a positive pressure filter module, and mainly filters a small amount of sample liquid, for example, the sample liquid contained in the filter column. The second filter module 8 is a screen filter module that filters mainly a large amount of sample liquid, for example, a sample liquid contained in a concentration bottle. It will be appreciated that the embodiments are not limited to two filter modules, nor to the type of filter module, and that one or more filter modules may be provided according to the filtering requirements, and that the filter modules may be screen filter modules or other types of filter modules.

Fig. 3 is a perspective view of the positive pressure filter module 7 according to an embodiment of the present disclosure, and fig. 4 is a partial enlarged view of a region near the filter jaw of the positive pressure filter module 7 according to an embodiment of the present disclosure. Referring to fig. 3 and 4, the positive pressure filter module 7 includes a base 701, and a moving mechanism 711 is provided on the base 701 to move the base 705 in a first direction, which may be a direction parallel to the base 701. The bottom plate 705 is provided with a tray for placing the liquid receiving bottle 713, a tray for placing the filter head, a pulling mechanism 712, and the like. The base 701 is vertically provided with a bracket 702, the bracket 702 is provided with a first support plate 703 and a second support plate 704, the first support plate 703 and the second support plate 704 vertically intersect, and the second support plate 704 is fixed on the first support plate 703 and can move on the first support plate 703 along a second direction, and the second direction is perpendicular to the first direction.

A second lifting mechanism 707 is fixedly connected to the second support plate 704, and the second lifting mechanism 707 can move up and down along the second support plate 704 by a driving mechanism. The second lifting mechanism 707 is further fixedly connected to the first lifting mechanism 706, and the first lifting mechanism 706 is fixedly connected to the air inlet joint 709 for driving the air inlet joint 709 to lift. The gas inlet joint 709 is connected to a gas source, not shown, and gas is supplied from the gas source to the gas inlet joint 709. A filter claw 708 is also fixedly connected to the second lifting mechanism 707 for holding a container, such as a filter column 710, which holds the liquid to be filtered. The second lifting mechanism 707 drives the filtering jaw 708, the air inlet joint 709, and the first lifting mechanism 706 together, and the filtering jaw 708, the air inlet joint 709, and the first lifting mechanism 706 may also move in a second direction on the first support plate 703.

The filtering column 710 contains the sample liquid to be filtered, and a plug is arranged below the filtering column 710 to prevent the liquid from leaking out in advance before filtering, and a filtering head can be arranged below the filtering column 710 first and then the plug can be arranged. The removing mechanism 712 for removing the plug is arranged on the bottom plate 705, a removing plate is arranged in the removing mechanism 712, a clamping groove is arranged on the removing plate, and a recovery mechanism is arranged below the removing mechanism 712. The filter claw 708 can clamp the filter column 710 and move to the position of the pulling mechanism 712, then the filter claw 708 clamps the filter column 710 to descend, the plug below the filter column 710 is clamped in the clamping groove, then the filter claw 708 clamps the filter column 710 to move upwards, the plug is pulled off, and the plug falls into the recovery mechanism below.

When filtering, the filtering clamping jaw 708 clamps the filtering column 710, the first lifting mechanism 706 drives the air inlet joint 709 to descend, and the air inlet joint 709 is connected with the filtering column 710. The second support plate 704 drives the filter clamping jaw 708 and the filter column 710 to move above the pulling out mechanism 712, the second lifting mechanism 707 drives the filter clamping jaw 708 to clamp the filter column 710 to descend, the plug of the filter column 710 is clamped in the clamping groove of the pulling out mechanism 712, and then the second lifting mechanism 707 drives the filter clamping jaw 708 and the filter column 710 to ascend to pull out the plug of the filter column 710.

When the filter head is arranged on the filter column 710, the filter head does not need to be pricked, and when the filter head is not arranged on the filter column 710, the second supporting plate 704 drives the filter clamping jaw 708 and the filter column 710 to move to the position where the filter head is placed, and the filter head is pricked. In the case of a sample volume of 0-2ml, the Tip head needs to be pricked at the outlet of the filter head, and the sample is pierced and filtered into a liquid phase vial. If the sample amount is 2-30ml, the Tip head is not required to be pricked, and the sample is directly filtered into a test tube. Then, the second supporting plate 704 drives the filtering clamping jaw 708 and the filtering column 710 to move above the liquid receiving bottle 713, the second lifting mechanism 707 drives the filtering clamping jaw 708 to clamp the filtering column 710 to descend, the filtering column 710 is in butt joint with the liquid receiving bottle 713, then an air source is started, the air source is communicated with the filtering column 710 through the air inlet joint 709, air pressure is introduced into the filtering column 710, and the sample liquid in the filtering column 710 is filtered through the air pressure. After the filtration is completed, the moving mechanism 711 drives the recovery mechanism to move to the position right below the filtering clamping jaw 708, the first lifting mechanism 24 drives the air inlet joint 709 to be separated from the filtering column 710, the filtering clamping jaw 708 releases the filtering column 710, and the filtering column 710 and the filtering head fall into the recovery mechanism.

Fig. 5 is a perspective view of a screen filter module according to an embodiment of the present disclosure, and fig. 6 is a rear view of a screen filter module according to an embodiment of the present disclosure. Referring to fig. 5 and 6, the screen filter module 8 includes a stand 801 vertically provided, a bottom plate 802 is provided at the bottom of the stand 801, a filter assembly support 803 is provided at a position below the center of the stand 801, a receiving bottle positioning frame 804 is provided at a position near the bottom of the stand 801, a lifting mechanism 805 is provided at an upper position of the stand 801, the lifting mechanism 805 includes a linear guide 806, a lifting assembly 807, and a lifting motor 808 provided at the back of the stand 801, and the lifting motor 808 drives the lifting assembly 807 to move up and down along the linear guide 806. The lifting assembly 807 is connected to the sealing platen 809, and drives the sealing platen 809 to move up and down, and a spring 813 is provided in the lifting assembly 807 for buffering pressure when moving up and down. The sealing platen 809 is provided with an air inlet joint 816, the air inlet joint 816 is connected to the air pipe 810, the air pipe 810 is connected to an air source not shown, a pressure regulating valve 811 and a throttle valve 812 for regulating the pressure of air in the air pipe 810 are further provided on the air pipe 810, and the air pipe 810 is also fixed to the bracket 801 by fixing the pressure regulating valve 811 and the throttle valve 812 to the bracket 801.

When filtering, the handling arm 3 drives the handling tool 4 to place the receiving bottle 814 on the receiving bottle positioning frame 804, places the filtering component 815 such as a funnel with a filter screen on the filtering component support 803, the filtering component 815 is in butt joint with the receiving bottle 814, and the sample liquid to be filtered is poured into the funnel, the lifting component 807 drives the sealing pressing plate 809 to descend, the sealing pressing plate 809 is in sealing connection with the funnel, the air source is opened, the sealing pressing plate 809 is communicated with the air source and the funnel through the air inlet connector 816, air pressure is introduced into the funnel, and the sample liquid in the funnel is filtered through the air pressure.

Optionally, a buffer glue block 817 and a liquid leakage preventing disc 818 may be further disposed below the receiving bottle positioning frame 804, the buffer glue block 817 is used for fixing and protecting the receiving bottle 814, and the liquid leakage preventing disc 818 is used for containing the spilled liquid when pouring the sample liquid, so as to avoid the liquid from polluting the working table surface.

In some embodiments, the filter modules 7 and 8 are juxtaposed near the second, rear side 112 of the table 11, but they may also be disposed near the third, left side 112 of the table 11. The position of the transport mechanism 20 may be arbitrarily set as long as the transport mechanism is provided around the transport mechanism to facilitate the transfer of the containers and/or trays from the exchange unit or the storage rack and the transfer of the containers and/or trays from the filtration module to the next process.

The high-pressure sample preparation and feeding module 5 is disposed at the substantially center of the stage 11 near the first or front side 111, and is adjacent to the handling mechanism 20 to facilitate the transfer of samples by the handling mechanism 20. In some embodiments, two high-pressure sample inlet and outlet modules 5 are provided in a direction from the first side 111 to the second side 112, and the two high-pressure sample inlet and outlet modules 5 are staggered a distance in the direction along the first side 111, and a storage rack 2 is provided between one high-pressure sample inlet and outlet module 5 closer to the fourth side 114 and the handling mechanism 20. Since the filtered samples in the filtration modules 7 and 8 are required to be transferred to the high-pressure sample inlet and outlet module 5 and the samples processed in the high-pressure sample inlet and outlet module 5 are required to be transferred to a pipetting module 9 which will be described later in the sample processing process, the storage rack 2 is arranged between the high-pressure sample inlet and outlet module 5 and the carrying mechanism 20, so that the sample containers or trays can be stored conveniently when the samples are transferred between the modules. Of course, the positions of the high-pressure sample inlet and outlet module 5 and the storage rack 2 can be adjusted according to the actual processing requirements.

Fig. 7 is a perspective view of a high-pressure sample loading and unloading module according to an embodiment of the present disclosure, fig. 8 is a perspective view of a fraction collecting drawer unit and a fraction collecting lifter unit in the high-pressure sample loading and unloading module according to an embodiment of the present disclosure, and fig. 9 is a side view of a fraction collecting drawer unit and a fraction collecting lifter unit in the high-pressure sample loading and unloading module according to an embodiment of the present disclosure.

The high-pressure sample inlet and outlet module 5 comprises a fraction collecting unit 501, a fraction collecting drawing unit 502 and a fraction collecting lifting unit 503. Fraction collecting unit 501 includes base 514, side wall 515 extending upward from base 514 and vertically disposed, top cover 516 connected to side wall 515, base 514, side wall 515 and top cover 516 enclosing a concave space as a fraction collecting operation space. The fraction collection unit 501 includes a sample injection needle, not shown, for introducing a sample liquid to be subjected to fraction collection, and a sample discharge needle, from which the sample liquid is discharged to a corresponding fraction collection container after the fraction is collected. Correspondingly, a sample injection position 511 is arranged at a position corresponding to a sample injection needle of the fraction collecting and pulling unit 501 in the fraction collecting and pulling unit 502 and used for placing a test tube or a solvent bottle filled with sample liquid to be processed, and a fraction collecting position 512 is arranged at a position corresponding to a sample outlet needle of the fraction collecting and pulling unit 501 in the fraction collecting and pulling unit 502 and used for placing a test tube or a solvent bottle for collecting fractions. The sample injection needle of the fraction collecting unit 501 is liftable, so that the sample injection position 511 of the fraction collecting drawing unit 502 is fixed, and the sample discharge needle of the fraction collecting unit 501 is fixed, so that the fraction collecting position 512 of the fraction collecting drawing unit 502 needs to be arranged to be liftable and capable of being docked with the sample discharge needle of the fraction collecting unit 501.

As shown in fig. 7, the fraction collecting position 512 of the fraction collecting and pulling unit 502 is located in a space surrounded by the base 514, the side wall 515 and the top cover 516 of the fraction collecting unit 501, where a test tube and a solvent bottle are placed, after the fraction collecting treatment is completed, the fraction collecting position 512 can be pulled out by the carrying mechanism 20, the test tube and the solvent bottle are removed, and then the fraction collecting position 512 is pushed back. Fraction collecting and lifting unit 503 is located outside the space surrounded by base 514, side wall 515, and top cover 516 of fraction collecting unit 501.

Referring to fig. 8 and 9, the fraction collecting and drawing unit 502 includes a sample introduction position 511 and a fraction collecting position 512, and a first slide rail 504 connected to the sample introduction position 511 to ensure smooth drawing of the sample introduction position 511. As shown in fig. 8, a support plate is additionally disposed beside the sample introduction position 511, and a first slide rail 504 is disposed on the support plate. A second slide rail 505 is provided corresponding to the fraction collecting position 512, and the second slide rail 505 is fixed to the first bottom plate 507. By providing the carrying tool 4 with a claw, the pulling plate 506 can be hooked, and the pulling plate 506 can be biased, so that the fraction collecting position 512 can be pulled out or pushed back along the second slide rail 505. The first bottom plate 507 is provided with limiting blocks 517 at the end positions corresponding to the second sliding rails 505, respectively, and the fraction collecting positions 512 are provided with limiting grooves 518. When the fraction collecting position 512 is pulled out along the second sliding rail 505, the limiting block 517 can be engaged with the limiting groove 518, so that the fraction collecting position 512 is temporarily fixed on the second sliding rail 505, so as to facilitate the carrying mechanism 20 to place or take away the container. After the operation is completed, the pulling plate 506 is forced to release the engagement between the limiting block 517 and the limiting groove 518, so that the fraction collecting position 512 can move along the second sliding rail 505.

A fraction collecting and lifting unit 503 is provided below the first bottom plate 507 to which the second slide rail 505 is fixed. The plurality of support rods 508 support the second bottom plate 510, a driving motor 513 is disposed below the second bottom plate 510, the lifting rod 509 is connected to the first bottom plate 507, and the driving motor 513 can drive the lifting rod 509 to move up and down, so as to drive the first bottom plate 507 to move up and down. One of the fraction collecting position 512 and the sample feeding position 511 is provided with a protruding member, the other is provided with a clamping hole, when the fraction collecting position 512 is lifted, the protruding member is separated from the clamping hole, and the fraction collecting position 512 and the sample feeding position 511 are separated. When the fraction collecting position 512 is lowered, the protruding member is inserted into the engagement hole, and the fraction collecting position 512 is connected to the sample introduction position 511. When the fraction collection site 512 and the sample introduction site 511 are connected, drawing can be performed together. Here, the protruding member may be a pin, the engagement hole may be a pin hole, or other components capable of being engaged with each other may be provided, so long as the engagement and disengagement with each other are satisfied, and the present invention is not limited thereto. When pulled, the clamping jaw assembled by the handling mechanism 20 hooks the pulling plate 506, so that the fraction collecting station 512 and the sample feeding station 511 move together.

In some embodiments, the high pressure sample access module 5 may be compatible with a plurality of different container trays, and different containers may be placed for collecting fractions condensed at different time periods or different temperatures for subsequent analysis or use. In some embodiments, a set of sample feeding positions and three sets of fraction collecting positions are arranged in the high-pressure sample feeding and discharging module 5, the sample feeding positions are used for placing a container tray filled with samples for sample feeding, and the fraction collecting positions are used for placing an empty bottle tray for fraction collecting.

In the prior high-pressure preparation equipment, the products after sample and fraction collection are manually taken and placed, and corresponding trays are manually selected and are operated and set on software for different types of sample containers, such as test tubes or pore plates with different specifications. The high-pressure sample inlet and outlet module can meet the automatic operation of different types of samples, can be matched with the carrying mechanism to grab and pull, achieves automatic inlet and outlet of products after samples and fractions are collected, can be lifted and docked with a control system of equipment, can automatically achieve automatic adaptation and switching of different types of containers, and avoids risks brought by manual misoperation and setting while improving efficiency.

Referring to fig. 1 and 2, a pump valve analysis module 6 is provided between the high pressure sample access module 5 and the fourth side 114 of the platen 11. In some embodiments, two pump valve analysis modules 6 are also provided corresponding to the two high pressure sample inlet and outlet modules 5 provided. The pump valve analysis module 6 is connected to the high-pressure sample inlet/outlet module 5 through a pipeline not shown. The pump valve analysis module 6 performs the relevant processing of pump fluid and analysis.

A mobile phase buffer module 10 is provided near the fourth side 114 of the table 11, and referring to fig. 1,2 and 13, the mobile phase buffer module 10 may be a separate part from the table 11 at a height lower than that of the table 11 so as not to affect the operation on the table 11. The bottom of the mobile phase buffer module 10 is provided with a plurality of rollers 1003, and the rollers 1003 can be unidirectional or universal rollers, can roll on the ground, and are convenient for the mobile phase buffer module 10 to move. The mobile phase caching module 10 is used for caching mobile phases required in the placement experiment process.

The pump valve analysis module 6 is also connected to the mobile phase buffer module 10 via a pipeline, not shown. The pump valve analysis module 6 introduces the samples in the high-pressure sample preparation and inlet and outlet module 5 through a pipeline, namely, a group of sample injection in the high-pressure sample preparation and inlet and outlet module 5, and introduces the preset mobile phase in the mobile phase buffer module 10 according to the introduced samples, and the samples are mixed with the preset mobile phase. The pump valve analysis module 6 separates components of the mixed solution, analyzes each component, and leads out different components to the high-pressure sample preparation and feeding module 5 through a pipeline for fraction collection, namely, collecting multiple groups of fractions in the high-pressure sample preparation and feeding module 5.

Referring to fig. 1 and 2, a pipetting module 9 is further provided near the second, rear side 112 of the table 11, the pipetting module 9 being used for pipetting. After the collection of a batch of high-pressure fractions in the high-pressure sample inlet and outlet module 5 is completed, the handling mechanism 20 is matched with the handling tool 4 to draw out the fraction collection position of the high-pressure sample inlet and outlet module 5, the test tube tray with the fractions collected is transferred to the pipetting module 9, the pipetting module 9 sucks samples from the fraction test tubes, and the samples are transferred into the liquid phase vials for the next process.

Fig. 10 is a perspective view of a pipetting module 9 of an embodiment of the disclosure. The pipetting module 9 comprises a holder 901, a pipetting assembly 904 and a de-heading assembly 905. The support 901 serves as a structural support foundation, and the pipetting assembly 904 and the de-heading assembly 905 are mounted on the support 901. The pipetting assembly 904 is configured to be coupled to the pipette tip 906 and to control pipetting and pipetting of the pipette tip 906, and the retraction assembly 905 is configured to separate the pipette tip 906 from the pipetting assembly 904 to effect retraction of the tips.

The bracket 901 includes a support riser 902 and a support cross plate 903 connected, the support riser 902 may be provided as a flat plate extending in a vertical direction, and the support cross plate 903 may be provided as a flat plate extending in a horizontal direction. The pipetting module 9 may further comprise a housing, where the housing is connected to the support riser 902 and the support cross plate 903, and the housing can enclose and shield at least part of the pipetting assembly 904, the head withdrawal assembly 905, etc. together with the support riser 902 and the support cross plate 903, so as to reduce exposed parts as much as possible, so that the pipetting module 9 has a complete and uniform appearance on the one hand, and has functions of dust prevention, water prevention, etc. on the other hand.

The pipetting assembly 904 may be integrally disposed on the support riser 902 or may be disposed mostly on the support riser 902 with the remainder disposed on the support cross plate 903. The pipetting assembly 904 is adapted to be removably coupled to the pipette tip 906, and the pipetting assembly 904 is also adapted to drive the pipette tip 906 to aspirate or spit fluids. The handling mechanism 20 may drive the rack 901 and the pipetting assembly 904 and the de-pipetting assembly 905 thereon to move integrally, wherein the pipetting assembly 904 may be connected to the suction head 906 after moving. The specific structure of the pipetting assembly 904 is not limited and can provide positive or negative pressure. By applying negative pressure to the suction head 906, the suction head 906 can be made to suck the sample liquid, the liquid sucking operation can be realized, and by applying positive pressure to the suction head 906, the sample liquid in the suction head 906 can be pushed out, and the liquid discharging operation can be realized.

A retraction assembly 905 is provided on the support cross plate 903, the retraction assembly 905 being adapted to urge the suction head 906 away from the pipetting assembly 904. The specific structure of the retraction assembly 905 is not limited, and the retraction assembly 905 may be moved to disengage the suction head 906 from the pipetting assembly 905 to effect a retraction operation.

The support 901 is further provided with a quick-change connector 907, and the quick-change connector 907 may be disposed on the supporting cross plate 903, for detachable connection with the handling mechanism 20, where the specific structure of the quick-change connector 907 is not limited, and the detachable connection manner includes, but is not limited to, pneumatic connection, magnetic connection, and the like. By the quick-change connector 907, quick connection and disconnection with the quick-change connector on the handling mechanism 20 can be realized, and the automation integration level is high.

When pipetting operation is performed, the handling mechanism 20 drives the pipetting module 9 to integrally move to the placing position of the suction head 906, so that the pipetting module 904 is connected with the suction head 906 at the placing position of the suction head 906, for example, the handling mechanism 20 drives the pipetting module 904 to prick down the suction head 906 with a certain force, so that the pipetting module 904 is in interference connection with the suction head 906, then drives the pipetting module 9 and the suction head 906 to move to the initial position, the pipetting module 904 drives the suction head 906 to suck liquid, and then the handling mechanism 20 drives the pipetting module 9 and the suction head 906 to move from the initial position to the target position again, and the pipetting module 904 drives the suction head 906 to spit liquid at the target position. Thus, once the pipette tip 906 is installed and the pipetting and pipetting steps are completed, one pipette tip 906 may perform pipetting and pipetting operations a plurality of times until the desired pipetting capacity for one sample fluid is completed. Then, when another sample liquid needs to be pipetted, the pipette tips 906 need to be replaced, and at this time, the carrying mechanism 20 drives the pipetting module 9 and the pipette tips 906 to move to the recovery position, and the pipette tip assembly 905 moves to push out the pipette tips 906 connected with the pipetting assembly 904, so that a pipette tip withdrawing operation is realized. The above-described operations of mounting the pipetting assembly 904 to the pipette tip 906 and pipetting and spitting are repeated.

Referring to fig. 1 and 2, the interaction unit 1, the housing rack 2, the high-pressure sample inlet and outlet module 5, the positive pressure filter module 7, the screen filter module 8, and the pipetting module 9 are disposed around the handling mechanism 20, and since these modules are modules that need to interact with the handling mechanism 20, they need to be enclosed within the range in which the handling mechanism 20 is operable, and modules that do not need to interact with the handling mechanism 20, for example, a pump valve analysis module may be disposed away from the handling mechanism 20. Through such position setting, not only can the efficiency of automation mechanized operation be guaranteed, but also the space can be saved to reasonable layout.

The mobile phase buffer module 10 is used for buffering a large volume of mobile phase liquid, and may include a plurality of mobile phase buffer tanks, and fig. 11 is a perspective view of one mobile phase buffer tank. As shown in fig. 11, a roll-over door 1001 is provided on the housing of the mobile phase buffer tank, in which a large-capacity tub, such as a 30L large-capacity tub, containing mobile phase is placed. When the liquid in the tub is insufficient, the roll-over door 1001 is manually opened by a person to take out the tub therein to add the liquid. The inner barrel communicates with the pump valve module 6 through a pipe via a through hole 1002 in the casing of the tank. In the prior art, the structure capacity of the mobile phase buffer memory module is smaller, and the liquid needs to be added manually and frequently. The bottom of mobile phase buffer storage case is provided with a plurality of gyro wheels 1003, and gyro wheel 1003 can be unidirectional or universal gyro wheel, can roll on subaerial, makes things convenient for mobile phase buffer storage module 10 to remove. The mobile phases stored in the different buffer tanks may be different. Any number of buffer tanks can be included in the mobile phase buffer module 10, so that a large volume of mobile phase liquid can be stored, so that the required mobile phase liquid can be continuously and uninterruptedly supplied during long-term experiments for 24 hours and 48 hours, and the middle interruption is not needed to supplement the liquid to influence the experimental process. The number of the buffer boxes in the mobile phase buffer module 10 is not limited as long as the requirements of the experiment can be satisfied.

Fig. 12 is a perspective view from the front side in a state where the automatic filtration and high-pressure separation and purification apparatus of the embodiment of the present disclosure is covered, and fig. 13 is a perspective view from the rear side in a state where the automatic filtration and high-pressure separation and purification apparatus of the embodiment of the present disclosure is covered. Here, the front surface means a side close to the user in a natural use state, that is, a front side of the table 11, a side where the interactive unit 1 is provided, and the rear surface means a side opposite to the front surface.

In some embodiments, the automated filtration and high pressure separation and purification apparatus 100 may include a housing 200, the housing 200 being disposed on the table 11, an interior receiving space being defined between the housing 200 and the table 11 in which the various process modules described above are located. Optionally, the cover 200 includes a frame 600, where the frame 600 is disposed on the table 11, and the table 11 and the frame 600 may be a detachable connection structure or an integral structure, which is not limited herein. In some embodiments, only the table 11 may be provided without the frame 600.

The inner receiving space is defined by the frame structures of the table 11 and the frame 600, i.e., the upper surface of the table 11 and the enclosure of the frame 600 form the inner receiving space. Optionally, the interior of the table 11 also has a receiving space. In the case where the cover 200 has a plurality of sub-frames, each sub-frame may have its own sub-space, and among the plurality of sub-spaces, some sub-spaces may communicate with each other, and some sub-spaces may be relatively independent and not communicate with other sub-spaces, which is not limited herein.

The frames 600 are connected by a plate-like structure to form the cover 200. The material of the cover 200 may be steel, aluminum alloy, or the like, and is not limited thereto. The overall shape of the cover 200 may be rectangular or any other feasible shape, and is not limited herein. The cover 200 may include a plurality of support risers, support beams, etc. to form a frame structure.

On the front side of the cover 200, interactive windows 300 and 301 are provided, and the interactive windows 300 and 301 communicate with the internal accommodating space and the external space. The interactive window 300 shown in fig. 12 is in an open state, and the interactive window 301 is in a closed state. In a state where the interactive window 300 is opened, the interactive unit 1 may interact with the outside. The mobile device moves to the interaction window 300 to place the transported material containers and/or trays to the interaction unit 1 or to take away the processed sample containers and/or trays placed on the interaction unit 1. Because the interactive window 300 is mainly used for interaction between the internal accommodating space and the external space, for convenience of interaction, the interactive window 300 is arranged in a sliding window form capable of moving up and down, and can be temporarily fixed when moving upwards to an open state, so that the open state of the window is maintained, and interaction between the internal accommodating space and the external space is not hindered.

The maintenance window 400 is provided on the cover 200 on the right side of the automatic filtering and high-pressure separating and purifying apparatus 100 shown in fig. 12, i.e. on the right side of the workbench 11, the maintenance window 400 is mainly used for a worker to maintain the module in the internal accommodating space, and for convenience of the worker to operate, the maintenance window 400 is provided in a form of a flat-open window, so that more internal accommodating space can be exposed when the maintenance window 400 is opened, and convenience is brought to the worker to maintain. Referring to fig. 13, maintenance windows 401, 402 and 403 in the form of flat-open windows are also provided on the cover on the left and rear sides of the automatic filtration and high pressure separation and purification apparatus 100, and a worker can open the maintenance windows to perform maintenance on the modules in the internal accommodating space.

An air duct assembly 500 is further provided at the top of the cover 200 for controlling the operating temperature of the inner accommodating space and replacing air.

Optionally, in one embodiment, the present application further provides an automated separation and purification preparation system, including a mobile device and at least one automated filtration and high pressure separation and purification device 100, the mobile device being configured to deliver material to the automated filtration and high pressure separation and purification device 100 and to remove samples processed by the automated filtration and high pressure separation and purification device 100.

For example, in the preparation stage of the experiment, the mobile device transfers various materials required by the experiment, such as trays containing empty test tubes, filter head trays, tip head trays, empty liquid phase vial trays and the like, to the interaction unit 1 of the automatic filtering and high-pressure separation and purification device 100, the sample liquid filtered by the first filtering module 7 or the second filtering module 8 can be transferred to the interaction unit 1 by the carrying mechanism 20 and then is conveyed to other working procedures by the mobile device, and the sample liquid subjected to fraction collection by the high-pressure preparation sample inlet and outlet module 5 and then is subjected to liquid transfer by the liquid transfer module 9 can be transferred to the interaction unit 1 by the carrying mechanism 20 and then is conveyed to other working procedures by the mobile device. The mobile device may be any mobile device known in the art that can perform the functions described above, such as, but not limited to, a mobile AGV or mobile robot.

Example embodiments have been disclosed herein, and although specific terms are employed, they are used and should be interpreted in a generic and descriptive sense only and not for purpose of limitation. In some instances, it will be apparent to one skilled in the art that features, characteristics, and/or elements described in connection with a particular embodiment may be used alone or in combination with other embodiments unless explicitly stated otherwise. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the disclosure as set forth in the appended claims.

Claims (24)

1. An automated filtration and high pressure separation and purification apparatus comprising:

The system comprises a workbench, a first filtering module, a second filtering module, a high-pressure sample inlet and outlet module, a pump valve analysis module and a carrying mechanism which are arranged on the workbench, and a mobile phase buffer module which is arranged independently of the workbench,

The first filtering module is used for filtering the sample liquid according to a first filtering requirement;

The second filtering module is used for filtering the sample liquid according to a second filtering requirement;

the sample inlet and outlet module is arranged at the high pressure, sample liquid filtered by the first filtering module or the second filtering module is led in, fraction collection is carried out, and the sample liquid and a product after fraction collection can be automatically inlet and outlet through the carrying mechanism;

The carrying mechanism is used for transferring materials between the first filtering module, the second filtering module and the high-pressure sample inlet and outlet module;

a mobile phase buffer module for buffering and placing mobile phase, and

And the pump valve analysis module is respectively connected with the high-pressure preparation sample inlet and outlet module and the mobile phase buffer module for pumping liquid and analyzing treatment.

2. The automated filtration and high-pressure separation and purification apparatus according to claim 1, the automatic filtration and high-pressure separation and purification device is characterized by further comprising:

An interaction unit for interacting with the outside through the carrying mechanism, and

And the pipetting module is used for transferring the sample liquid collected by the fraction into a preset container according to a preset pipetting capacity.

3. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein,

The pump valve analysis module is used for introducing the sample liquid from the high-pressure preparation sample inlet and outlet module, introducing a mobile phase corresponding to the sample liquid from the mobile phase buffer module, carrying out component separation and analysis on the mixed liquid of the sample liquid and the mobile phase, and leading out different components to the high-pressure preparation sample inlet and outlet module.

4. The automated filtration and high pressure separation and purification apparatus of claim 2, further comprising a housing rack for holding materials required for processing by each module,

The interaction unit, the storage rack, the first filtering module, the second filtering module, the high-pressure preparation sample inlet and outlet module and the pipetting module are arranged around the carrying mechanism and located in an operable range of the carrying mechanism.

5. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein,

The mobile phase buffer module comprises a plurality of mobile phase buffer boxes, and a large-capacity barrel filled with mobile phase is placed in each mobile phase buffer box.

6. The automated filtration and high-pressure separation and purification apparatus of claim 4, wherein,

The workbench comprises a front side edge, a rear side edge, a left side edge and a right side edge,

The interaction unit is arranged on the front side, the storage rack is arranged on the left side, the first filter module, the second filter module and the pipetting module are arranged on the rear side in parallel, the high-pressure sample preparation and feeding module is arranged between the front side and the rear side and is closer to the front side,

The pump valve analysis module is disposed adjacent to the high pressure preparation sample inlet and outlet module and remote from the handling mechanism.

7. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein,

The first filtering module is a positive pressure filtering module and is used for filtering a small amount of sample liquid,

The second filtering module is a filter screen filtering module and is used for filtering a large amount of sample liquid.

8. The automated filtration and high-pressure separation and purification apparatus of claim 7, wherein,

The positive pressure filtration module includes:

A clamping mechanism for clamping a filtration vessel containing a sample to be filtered;

The air inlet connector is positioned above the clamping mechanism and connected with an air source, and is used for communicating the air source with a sample to be filtered during filtering;

The first lifting mechanism is connected with the air inlet joint and used for driving the air inlet joint to lift;

The clamping mechanism and the first lifting mechanism are fixed on the second lifting mechanism, and the second lifting mechanism is used for driving the clamping mechanism, the air inlet joint and the first lifting mechanism to lift together;

a removing mechanism for removing the plug below the filter container, and

And the receiving bottle placing mechanism is used for placing a receiving bottle for receiving the filtered sample liquid.

9. The automated filtration and high pressure separation and purification apparatus of claim 8, wherein,

The positive pressure filtration module further comprises:

A base on which the extracting mechanism and the receiving bottle placing mechanism are arranged, and

The bracket is vertically arranged on the base, a first supporting plate and a second supporting plate are arranged on the bracket,

The first supporting plate and the second supporting plate are vertically crossed, the second supporting plate is fixed on the first supporting plate and can move along the first supporting plate, and the second lifting mechanism is fixedly connected to the second supporting plate.

10. The automated filtration and high-pressure separation and purification apparatus of claim 7, wherein,

The screen filtration module includes:

A vertically arranged bracket;

the receiving bottle positioning frame is arranged at the bottom of the bracket and is used for placing a receiving bottle for receiving the filtered sample liquid;

The filter component support frame is arranged above the receiving bottle positioning frame and fixed on the support frame and used for placing a filter component with a filter screen;

A lifting mechanism arranged on the bracket, and

The sealing pressing plate is connected with the lifting mechanism, the lifting mechanism drives the sealing pressing plate to lift, an air inlet connector is arranged on the sealing pressing plate and connected with an air source, and the air inlet connector is used for communicating the air source with the filtering assembly during filtering.

11. The automated filtration and high pressure separation and purification apparatus of claim 10, wherein,

The lifting mechanism comprises a lifting component, a linear guide rail and a motor,

The linear guide rail is fixed at the upper position of the bracket,

The lifting assembly is connected with the sealing pressing plate and driven by a motor to move up and down along the linear guide rail.

12. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein,

The high-pressure preparation sample inlet and outlet module comprises:

the fraction collecting unit comprises a sample injection needle and a sample discharge needle and is used for collecting fractions of the introduced sample liquid;

The fraction collecting and pulling unit comprises a sample injection position corresponding to the sample injection needle, a fraction collecting position corresponding to the sample outlet needle, and a component capable of pulling out or pushing in the sample injection position and the fraction collecting position from the fraction collecting unit, and

And the fraction collecting lifting unit is used for lifting the fraction collecting position.

13. The automated filtration and high pressure separation and purification apparatus of claim 12, wherein,

The sample injection needle of the fraction collecting unit is arranged to be liftable, the sample injection position of the fraction collecting and drawing unit is arranged to be fixed, the sample outlet needle of the fraction collecting unit is arranged to be fixed, and the fraction collecting position of the fraction collecting and drawing unit is arranged to be liftable.

14. The automated filtration and high pressure separation and purification apparatus of claim 12, wherein,

The fraction collecting and drawing unit comprises:

The sample injection position is used for placing a sample to be treated;

A fraction collection site for collecting fractions;

the sample injection position can move along the first slide rail;

A bottom plate;

a plurality of second slide rails fixed on the bottom plate, the second slide rails being arranged corresponding to the fraction collecting position, the fraction collecting position being movable along the second slide rails, and

The end of the fraction collecting position is provided with a drawing plate, and the fraction collecting position can be moved along the second sliding rail by applying force to the drawing plate.

15. The automated filtration and high pressure separation and purification apparatus of claim 14, wherein,

The second slide rail is provided with a limiting block, the fraction collecting position is provided with a limiting groove, and when the fraction collecting position is pulled out, the fraction collecting position is fixed on the second slide rail through the clamping of the limiting block and the limiting groove.

16. The automated filtration and high pressure separation and purification apparatus of claim 14, wherein,

The fraction collecting lifting unit is arranged below the bottom plate and comprises a driving motor and a lifting rod, the lifting rod is connected with the bottom plate, the driving motor drives the lifting rod to lift,

The fraction collecting position and the sample feeding position are respectively provided with a protruding piece and a clamping hole, when the fraction collecting position is lifted, the protruding piece is separated from the clamping hole, the fraction collecting position is separated from the sample feeding position, when the fraction collecting position is lowered, the protruding piece is inserted into the clamping hole, and the fraction collecting position is connected with the sample feeding position.

17. The automated filtration and high pressure separation and purification apparatus of claim 12, wherein,

The high-pressure preparation sample inlet and outlet module comprises a group of sample inlet positions and three groups of fraction collection positions.

18. The automated filtration and high pressure separation and purification apparatus of claim 2, wherein,

The pipetting module comprises:

the bracket comprises a supporting vertical plate and a supporting transverse plate which are connected;

A pipetting assembly arranged on the supporting vertical plate and detachably connected with the suction head, and used for driving the suction head to suck or spit liquid, and

The head withdrawing assembly is arranged on the supporting transverse plate and is used for pushing the suction head to be separated from the pipetting assembly.

19. The automated filtration and high pressure separation and purification apparatus of claim 2, wherein,

The interaction unit includes:

the bottom plate is arranged on the workbench;

The supporting plates are connected with the two ends of the bottom plate and are vertically arranged,

A placing plate connected between the supporting plates for storing material containers and/or trays,

The placing plate is provided with a plurality of first bin positions, each first bin position comprises a locating piece and a sensor, the locating pieces are used for limiting the material containers and/or the trays, and the sensors are used for detecting whether the bin positions store the material containers and/or the trays.

20. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein,

The handling mechanism comprises a handling arm and a handling tool, wherein the handling arm is a mechanical arm or a moving mechanism with at least one degree of freedom in one direction, one end of the handling arm is fixed on a workbench, the handling tool is mounted at the other end of the handling arm, and the handling tool is detachably connected with the handling arm.

21. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein,

The workbench is also provided with a tool bin for storing various conveying tools, and the conveying mechanism is used for taking and placing the conveying tools from the tool bin.

22. The automated filtration and high-pressure separation and purification apparatus of claim 4, wherein,

The storage rack comprises at least one storage plate and two vertically arranged supporting pieces, wherein the storage plate is arranged between the two supporting pieces, and a second bin for placing a container and/or a tray is arranged on the storage plate.

23. The automated filtration and high pressure separation and purification apparatus of claim 2, wherein,

A cover body is also arranged on the workbench, an internal accommodating space is formed between the cover body and the workbench,

The interactive device comprises an interactive unit, a cover body, a maintenance window and a module, wherein the interactive window is arranged on one side of the interactive unit, the cover body is used for carrying out material interaction with an external space, and the maintenance window is arranged on the other side surfaces of the cover body, and is used for maintaining the module in the internal accommodating space.

24. An automated separation and purification preparation system comprising a mobile device and at least one automated filtration and high pressure separation and purification device according to any one of claims 1-23, the mobile device being configured to deliver material to the automated filtration and high pressure separation and purification device and to remove samples processed by the automated filtration and high pressure separation and purification device.