CN222250744U

CN222250744U - On-orbit microorganism culture experimental load

Info

Publication number: CN222250744U
Application number: CN202420823357.9U
Authority: CN
Inventors: 张文德; 赵勋峰; 印红; 袁俊霞; 张秦; 徐侃彦; 杨金禄; 杨双; 童曈; 党磊; 马玲玲
Original assignee: Space Shenzhou Biology & Technology Group Co ltd
Current assignee: Space Shenzhou Biology & Technology Group Co ltd
Priority date: 2024-04-19
Filing date: 2024-04-19
Publication date: 2024-12-27
Anticipated expiration: 2034-04-19

Abstract

The utility model provides an on-orbit microorganism culture experiment load which comprises a load box body, wherein a box body space is formed in the load box body, a test module and a monitoring module are both arranged in the box body space, the test module is composed of a plurality of culture units, the monitoring module and the test module are oppositely arranged, the culture units comprise a culture pond, a storage pond, a turnover membrane, a transfusion pipeline and an air outlet pipeline, the storage pond is connected with the culture pond, the turnover membrane is arranged in the storage pond so as to divide an inner cavity of the storage pond into a sealed liquid storage cavity and an air storage cavity, a liquid inlet of the culture pond is connected with the liquid storage cavity through the transfusion pipeline, and an air outlet of the culture pond is connected with the air storage cavity through the air outlet pipeline. The on-orbit microorganism culture experimental load provided by the utility model can realize accurate control and monitoring of the microorganism culture process in a microgravity environment, and can provide high-flux experimental capability and ensure the safety and reliability of experiments through the integrated design.

Description

On-orbit microorganism culture experimental load

Technical Field

The utility model relates to the technical field of space technology tests, in particular to an on-orbit microorganism culture experimental load.

Background

The microorganism has the advantages of simple structure, short growth cycle, rapid propagation, convenient carrying and the like, and is used as a biological model for life phenomenon research in space environment, extraterrestrial life detection, planetary protection task research taking the microorganism as a focus object, and the like. With the construction of space stations in China, large-scale and multidisciplinary space science and technology tests are developed. The realization of microbial culture is an important premise for developing microbial research under space conditions, such as developing spatial microbial material corrosiveness research, spatial microbial mutagenesis research, drug resistance research and the like. Due to the specificity of the spatial environment, in-orbit microbial cultivation faces a series of problems.

Disclosure of utility model

The utility model provides an on-orbit microorganism culture experimental load which is used for solving the defects in the prior art and realizing the following technical effects that the accurate control and monitoring of the microorganism culture process in a microgravity environment can be realized so as to support the study of space biology, and the experimental load can provide high-flux experimental capability and ensure the safety and reliability of experiments through the integrated design.

According to an embodiment of the utility model, an on-orbit microorganism culture experimental load comprises:

The load box body is internally provided with a box body space;

The test module and the monitoring module are both arranged in the box body space, the test module consists of a plurality of culture units, and the monitoring module and the test module are oppositely arranged to take microorganism image data in the culture units;

The culture unit comprises a culture pond, a storage pond, a turnover membrane, a transfusion pipeline and an air outlet pipeline, wherein the storage pond is connected with the culture pond, and the turnover membrane is arranged in the storage pond so as to divide an inner cavity of the storage pond into a sealed liquid storage cavity and a sealed air storage cavity;

The liquid inlet of the culture pond is connected with the liquid storage cavity through the liquid delivery pipeline, and the gas outlet of the culture pond is connected with the gas storage cavity through the gas outlet pipeline.

According to one embodiment of the utility model, a heating element is arranged on the outer side of the culture pond, and a pump body is arranged on the infusion pipeline;

the on-orbit microorganism culture experimental load also comprises a control module, wherein the control module is respectively connected with the monitoring module, the heating element and the pump body.

According to one embodiment of the utility model, the monitoring module comprises a plurality of cameras and a plurality of diaphragms, one diaphragm is arranged on each camera, and the cameras and the diaphragms are connected with the control module.

According to one embodiment of the utility model, a mounting interlayer crossing the box space is arranged in the carrier box body, and the mounting interlayer divides the box space into a mounting cavity and a culture cavity;

The camera passes through the mounting interlayer, the camera body of the camera is positioned in the mounting cavity, the camera head of the camera is positioned in the culture cavity, the test module is mounted in the culture cavity, and the culture unit and the camera head are arranged oppositely.

According to one embodiment of the utility model, the outer wall surface of one side of the mounting interlayer, which faces the culture cavity, forms a mounting reference surface, the camera is fixed on the mounting reference surface, and all culture units of the test module are arranged opposite to the mounting reference surface.

According to one embodiment of the utility model, the control module is installed in an interlayer cavity inside the installation interlayer, the installation datum plane is further provided with a fixing hole communicated with the interlayer cavity, and the control module comprises a control end electric connector for realizing electric connection, and the control end electric connector is fixed in the fixing hole.

According to one embodiment of the utility model, the test module comprises a first test module, a second test module and a third test module, wherein the monitoring module comprises a first camera, a second camera and a third camera, and a first aperture, a second aperture and a third aperture are respectively arranged on the first camera, the second camera and the third camera;

The first test module, the second test module and the third test module are sequentially arranged along the length direction of the installation interlayer, and the first camera, the second camera and the third camera are also sequentially arranged along the length direction of the installation interlayer and are respectively in one-to-one correspondence with the first test module, the second test module and the third test module in position.

According to one embodiment of the utility model, the control module includes a power distribution computer processing circuit board, a drive control circuit board, and a control side electrical connector that are interconnected.

According to one embodiment of the utility model, the power distribution computer processing circuit board comprises a power distribution unit and a computer processing unit;

The power distribution unit comprises a fuse circuit, a surge suppression circuit, an EMI filter, a DC/DC converter, a camera power supply circuit and an aperture power supply circuit, wherein the camera power supply circuit and the aperture power supply circuit are respectively connected with the DC/DC converter;

The computer processing unit comprises an FPGA circuit, a CPU circuit, a watchdog circuit, an Ethernet PHY circuit, a camerlink receiver, an SDRAM external memory circuit and an EEPROM memory chip;

The CPU circuit is connected with the FPGA circuit, the watchdog circuit is connected with the CPU circuit and the FPGA circuit respectively, the Ethernet PHY circuit is connected with the FPGA circuit, the SDRAM external memory circuit is connected with the camerlink receiver and the FPGA circuit respectively, and the EEPROM memory chip is connected with the CPU circuit.

According to an embodiment of the present utility model, the driving control circuit board includes:

A filter circuit;

The pump body driving circuit is connected with the FPGA circuit;

And the heating element control circuit is connected with the FPGA circuit.

The on-orbit microorganism culture experimental load can realize the accurate control and monitoring of the microorganism culture process under the microgravity environment, thereby supporting the research of space biology, such as the research of corrosiveness of microorganism materials, the research of space microorganism mutagenesis, the research of drug resistance and the like. By such an integrated design, the experimental load can provide high throughput experimental capability while ensuring safety and reliability of the experiment.

Drawings

In order to more clearly illustrate the utility model or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the components of an on-orbit microbial culture experimental load provided by the utility model;

FIG. 2 is a schematic structural diagram of an on-orbit microorganism culture experimental load provided by the utility model;

FIG. 3 is one of the schematic perspective views of the experimental load for on-orbit microorganism culture provided by the utility model;

FIG. 4 is a second perspective view of the on-orbit microorganism culture experimental load provided by the utility model;

FIG. 5 is a third perspective view of the experimental load for in-orbit microorganism culture provided by the utility model;

FIG. 6 is a schematic diagram of a culture unit according to the present utility model;

FIG. 7 is a second schematic diagram of a culture unit according to the present utility model;

FIG. 8 is a schematic exploded view of the structure of the culture unit provided by the present utility model;

FIG. 9 is a schematic diagram of the structure of the culture pond provided by the utility model.

Reference numerals:

01. The test module comprises a 011, a first test module, 012, a second test module, 013, a third test module, 02, a monitoring module, 021, a first camera, 022, a second camera, 023, a third camera, 024, a first aperture, 025, a second aperture, 026, a third aperture, 03, a control module, 031, a power distribution computer processing circuit board, 032, a driving control circuit board, 033, a control end electric connector, 04, a load box, 041, a mounting cavity, 042, a culture cavity, 043, a mounting interlayer, 044 and a mounting reference surface;

1. A culture pond; 101, a first flanging, 102, a second flanging, 103, a second accommodating cavity, 104, a first supporting column, 1041, a connecting hole, 105, a second supporting column, 1051, a liquid inlet channel, 106, an air outlet hole, 107, a first connecting pipe, 108, a second connecting pipe, 109, a connecting column, 2, a storage pool, 201, a liquid storage cavity, 202, a third connecting pipe, 203, a fourth connecting pipe, 204, a pool body, 205, a cover body, 206 and a third connecting part;

3. The device comprises a turnover film, 301, a protruding part, 4, a first accommodating cavity, 5, a heating part, 6, a heat insulation block, 7, an electric connector, 8, a sample bracket, 801, an overflow gap, 9, a compression frame, 10, a window, 11, a pump body, 12, a heat insulation buffer cushion, 121, a fixing ring, 122, a first connecting part, 13, a wire bundle seat, 131, a first wire bundle hole, 132, a second wire bundle hole, 14, a connecting frame, 141, a second connecting part, 142 and a weight reducing hole.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the embodiments of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the embodiments of the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the embodiments of the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In embodiments of the utility model, unless expressly specified and limited otherwise, a first feature "up" or "down" on a second feature may be that the first and second features are in direct contact, or that the first and second features are in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the embodiments of the present utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

As shown in fig. 1 to 9, the on-orbit microorganism culture experimental load according to the embodiment of the present utility model includes an experimental module 01, a monitoring module 02 and a control module 03.

The test module 01 consists of a plurality of culture units, each culture unit is internally provided with a culture pond 1 and a heating piece 5 for heating the culture pond 1, and the culture pond 1 is connected with a transfusion pipeline with a pump body 11;

The monitoring module 02 is arranged opposite to the test module 01 in position, and the monitoring module 02 is used for carrying out static or dynamic imaging on the test module 01 so as to acquire microorganism growth information in the culture pond 1;

The control module 03 is respectively connected with the monitoring module 02 and the water pump and the heating element 5 in the test module 01, and the control module 03 is used for receiving the microorganism image data transmitted by the monitoring module 02 and controlling and adjusting the working state of the pump body 11 and/or the heating element 5 according to the microorganism image data.

It can be understood that the on-orbit microorganism culture experiment load of the utility model is a highly integrated system, which is specially designed for microorganism culture experiments in an on-orbit space environment and consists of three main parts:

The test module 01 is a core part of an on-orbit microorganism culture experiment load and consists of a plurality of culture units. Each culture unit comprises a culture tank 1 for holding a microbiological culture media. The culture tank 1 is connected with the pump body 11 through a liquid delivery pipe so as to deliver nutrient substances into the culture tank 1. Furthermore, each culture tank 1 is also provided with a heating member 5 for maintaining a suitable temperature to promote the growth of microorganisms.

The monitoring module 02 is located opposite to the test module 01 and is responsible for monitoring the test module 01 in real time. It captures the growth of microorganisms in the culture tank 1 by means of static or dynamic imaging techniques and transmits these image data to the control module 03. The monitoring module 02 may include a plurality of cameras and LED diaphragms to ensure that conditions within the culture pond 1 are clearly observed under different illumination conditions.

The control module 03 is the brain of the experimental load, which receives the image data from the monitoring module 02 and adjusts the operating states of the pump body 11 and the heating element 5 in the experimental module 01 based on these data. Thus, the control module 03 can realize accurate control of the microorganism culture environment and ensure smooth experiment. The control module 03 may include a computer processing circuit board, a drive control circuit board 032, a control end electrical connector 033, etc. to achieve overall control of experimental loads.

The specific working principle of the on-orbit microorganism culture experimental load according to the embodiment of the utility model is that the control module 03 receives an instruction, drives the pump body 11 through the drive control circuit board 032, and injects a proper amount of culture solution into the culture pond 1 of the culture unit. The control module 03 simultaneously controls the heating element 5, and adjusts the temperature of the culture pond 1 according to experimental requirements, so as to ensure a proper temperature environment required by microorganism growth. The camera in the monitoring module 02 carries out static and dynamic imaging on the test module 01, captures the growth condition of microorganisms in the culture pond 1, and the LED aperture provides illumination to ensure that the imaging is clear. The monitoring module 02 transmits the captured image data to the control module 03, the control module 03 receives the image data and may perform preliminary processing, such as compression, to reduce the amount of data transmission, and the control module 03 adjusts the operating states of the pump body 11 and the heating member 5 according to the image data transmitted back from the monitoring module 02 to optimize the microorganism growth conditions. During the experiment, the control module 03 may need to adjust the nutrient supply amount and the temperature according to the growth condition of the microorganism, so as to realize dynamic control.

It should be pointed out that the on-orbit microorganism culture experimental load can realize the accurate control and monitoring of the microorganism culture process under the microgravity environment, thereby supporting the research of space biology, such as the research of corrosiveness of microorganism materials, the research of space microorganism mutagenesis, the research of drug resistance and the like. By such an integrated design, the experimental load can provide high throughput experimental capability while ensuring safety and reliability of the experiment.

In conclusion, the on-orbit microorganism culture experimental load has the following advantages and particularities under the microgravity environment (1) the system integration, namely, the control, test and monitoring modules are integrated, the full-flow automation of the on-orbit microorganism culture experiment is realized, the dependence on the operation of astronauts is reduced, and the experimental efficiency is improved. (2) The high throughput experimental capability is that the microorganism culture of a plurality of independent culture units can be supported simultaneously, and it can be understood that the scheme can ensure independent temperature control and nutrient supply under the microgravity environment because the traditional gravity-dependent culture method is not applicable any more in the weightless state. (3) The monitoring module 02 can capture the growth condition of microorganisms in the culture pond 1 in real time, and dynamically adjust experimental conditions through the control module 03, so as to ensure the accuracy and reliability of experiments. It will be appreciated that the above described real-time feedback mechanism is particularly critical in a microgravity environment, as microgravity affects natural convection and sedimentation of the material. (4) And the temperature and the humidity are precisely controlled, the convection and sedimentation modes of the substances are changed in the microgravity environment, the experimental load is controlled by the precise temperature and the precise humidity, the microorganism growth environment on the earth is simulated, and the physiological change of microorganisms under the weightlessness condition can be studied. (5) The safety design is that the experimental load adopts a double-sealing design, the inner layer meets the requirement of liquid tightness, the outer layer meets the requirement of air tightness, and the biological safety of the experiment is ensured. Meanwhile, the high-temperature sterilization function can thoroughly sterilize the culture unit after the experiment is finished, and microbial contamination is prevented. (6) The experimental load adopts a modularized design, so that the experimental load can be quickly modified and upgraded according to different experimental requirements, and the flexibility and adaptability of on-orbit experiments are improved.

As shown in fig. 1 and 2, according to some embodiments of the present utility model, the monitoring module 02 includes a plurality of cameras and a plurality of diaphragms, one diaphragm being provided on each camera, and the cameras and diaphragms being connected to the control module 03.

And the control module 03 is used for receiving the microorganism image data transmitted by the camera and adjusting the working state of the aperture according to the microorganism image data.

In this embodiment, the monitoring module 02 is equipped with a plurality of cameras to facilitate simultaneous or sequential monitoring of a plurality of culture units in the test module 01. And each camera is provided with an aperture for adjusting the amount of light entering the camera to ensure image quality.

Wherein the aperture on each camera can be adjusted independently, which means that the illumination conditions of each cultivation unit can be precisely controlled according to the growth status and illumination requirements of the microorganisms in the cultivation unit. It will be appreciated that the adjustment of the aperture is critical for observing the growth of microorganisms in a microgravity environment, as the lighting conditions affect the metabolic activity and growth pattern of the microorganisms.

As shown in fig. 1, the control module 03 includes a power distribution computer processing circuit board 031, a drive control circuit board 032, and a control side electrical connector 033, which are interconnected according to some embodiments of the present utility model.

The power distribution computer processing circuit board 031 is used for performing power distribution control on the control module 03, the test module 01 and the monitoring module 02 and performing computer processing on microorganism image data, the driving control circuit board 032 is used for controlling working states of the pump body 11 and the heating element 5 in the test module 01, and the control end electric connecting element is used for being in telecommunication connection with the test module 01 and the monitoring module 02 respectively.

In the device of the utility model, the control module 03 is used for experimental load power supply and distribution control, driving control, communication control, image receiving, compression, storage and other control, intelligent temperature control of the test module 01, liquid conveying control of the test module 01 and other control.

The distribution computer processing circuit board 031 is mainly responsible for performing distribution control on the control module 03, the test module 01 and the monitoring module 02, so as to ensure that each module obtains stable power supply, and in addition, the distribution computer processing circuit board 031 is also responsible for performing computer processing on microorganism image data, specifically including image acquisition, storage, compression and transmission, and possible image analysis, so as to better understand the growth state of microorganisms.

The drive control circuit board 032 is used for controlling the pump body 11 and the heating element 5 in the test module 01, so as to realize accurate control of nutrient supply and temperature in the culture unit.

The control-side electrical connector 033, which serves as a telecommunications connection between the control module 03 and the test module 01 and the monitoring module 02, ensures the transmission of data and control signals. And the connector enables the control module 03 to communicate with the test module 01 and the monitoring module 02, so that remote monitoring and control of the experimental process are realized.

As shown in fig. 1, according to some embodiments of the utility model, the power distribution computer processing circuit board 031 includes a power distribution unit including a fuse circuit, a surge suppression circuit, an EMI filter, a DC/DC converter, a camera power supply circuit, and an aperture power supply circuit, etc. In this way, the power distribution unit, as a core of the power distribution computer processing circuit board 031, can convert the bus power supply to various voltage levels required for the experimental load for use by the control module 03, the experimental module 01 and the monitoring module 02.

In some embodiments, the fuse circuit includes two fuses connected in parallel, wherein a power resistor is connected in series to one branch, and the resistance of the power electrons is not less than 20 times of the resistance of the cold state resistor of the fuse. In this way, the fuse circuit protects the circuit from overload and short circuits, wherein the series power resistor on one leg helps to provide additional protection in the event of a sudden increase in current, ensuring circuit safety.

In some embodiments, the surge suppression circuit is built based on an N-channel switching transistor, and the surge control circuit controls conduction by controlling gate voltage and achieves the suppression function of the power-on surge by using a soft start mode. Thus, the surge suppression circuit can suppress surge current generated during power-on and realize a soft start function, thereby reducing impact on the circuit and protecting electronic equipment

In some embodiments, the EMI filter is used to filter noise and ripple, so that noise and ripple on the bus can be filtered, and reflection noise of the system is controlled to prevent interference to the bus.

In some embodiments, a DC/DC converter is used to convert the high voltage of the bus to a low voltage secondary power supply. The camera power supply circuit and the aperture power supply circuit are used for distributing power to the camera and the aperture respectively, and are connected with the DC/DC converter. Thus, the DC/DC converter converts the input high voltage (e.g., 100V bus power) to the low voltage (e.g., +5V, +12V, and +24V, etc.) required for the experimental load to provide the appropriate power for the camera power circuit and aperture power circuit, e.g., the camera power circuit uses +12V for power and the aperture power circuit uses +24V for power.

As shown in fig. 1, according to some embodiments of the present utility model, the power distribution computer processing circuit board 031 further includes a computer processing unit, and then the computer processing unit includes a CPU circuit, an FPGA circuit, a watchdog circuit, an ethernet PHY circuit, a CameraLink receiving circuit, an SDRAM external memory circuit, an EEPROM memory chip, and the like. It will be appreciated that the main functions of the computer processing unit are communication control, image reception, compression, storage etc. control.

In some embodiments, FPGA circuitry is used to accomplish ethernet communication control, image reception and compression, external memory control, switch control of pump body 11, and feedback system control of heater element 5. In this way, field Programmable Gate Array (FPGA) circuits are used to perform complex logic functions, and the flexibility of the FPGA allows it to be programmed to suit different tasks according to experimental requirements.

In some embodiments, the CPU circuitry is coupled to the FPGA circuitry for sending instructions to control the FPGA circuitry. It will be appreciated that the CPU circuitry is the core of the overall system, processes instructions from the ground control center, and makes decisions based on experimental requirements.

In some embodiments, the watchdog circuit is connected to and used to hardware reset the CPU circuit and the FPGA circuit, respectively. In this embodiment, the purpose of the watchdog circuit is to set up a security mechanism for monitoring the running states of the CPU and the FPGA, specifically, if an abnormality is detected, the watchdog circuit triggers a hardware reset to ensure stable operation of the system.

In some embodiments, the ethernet PHY circuitry is coupled to the FPGA circuitry for implementing an ethernet communication protocol. In this way, the Ethernet PHY circuit belongs to a physical layer Ethernet interface, and can realize an Ethernet communication protocol by being connected with the FPGA circuit, so that the experimental load can be ensured to exchange data with a network system of a space station or other equipment.

In some embodiments, camerlink receivers are used to receive the microorganism image data acquired by the camera.

In some embodiments, SDRAM external memory circuitry is coupled to camerlink receiver and FPGA circuitry, respectively, for caching microbiological image data.

In some embodiments, an EEPROM memory chip is connected to the CPU circuitry and is used to store the CPU program. It will be appreciated that EEPROM (electrically erasable programmable read only memory) is a non-volatile memory that can retain data after power down, ensuring that the correct program can be loaded when the system is started.

In summary, by the co-operation of the various components within the computer processing unit described above, the power distribution computer processing circuit board 031 is enabled to efficiently handle and control various operations of the experimental load, including data acquisition, communication, storage, and precise control of experimental conditions. The design not only improves the intelligent level of experimental load, but also enhances the reliability and flexibility of the experimental load in a complex space environment.

As shown in fig. 1, the drive control circuit board 032 includes a pump body drive circuit, a filter circuit, and a heater control circuit according to some embodiments of the present utility model.

The pump body driving circuit is connected with the FPGA circuit, and the FPGA circuit controls the on-off state of the pump body 11 and the operation parameters thereof through the level conversion circuit. The heating element control circuit is connected with the FPGA circuit, and the FPGA circuit controls the switch and the heating parameters of the heating element 5 through the level switching circuit.

In this embodiment, the main function of the driving control circuit board 032 is to drive the pump body 11 and the heating element 5 in the test module 01 to open and close, and to control the parameter states of the two, so as to complete the liquid injection and temperature control of each culture unit in the test module 01.

The pump body driving circuit is connected with the FPGA circuit and is responsible for controlling the starting and stopping of the pump body 11 and adjusting the operation parameters of the pump body 11, such as flow and pressure, so as to realize the accurate control of nutrient supply in the culture unit. The filter circuit is used for stabilizing power supply, reducing power noise and ensuring that the pump body 11 and other sensitive electronic equipment can work stably. The heating element control circuit is also connected with the FPGA circuit and is responsible for controlling the switching state and heating parameters of the heating element 5, such as temperature setting and heating power, so as to realize accurate control of the heating element 5 and ensure that the temperature of the culture environment is stabilized in a range suitable for microorganism growth.

As shown in fig. 2-5, the in-orbit microorganism culture experiment load further comprises a load box 04 according to some embodiments of the utility model. The load box 04 is internally provided with a box space, a mounting interlayer 043 crossing the box space is arranged in the carrier box, and the mounting interlayer 043 divides the box space into a mounting cavity 041 and a culture cavity 042.

The control module 03 is arranged in an interlayer cavity in the installation interlayer 043, the camera penetrates through the installation interlayer 043, the camera body of the camera is positioned in the installation cavity 041, the camera head of the camera is positioned in the culture cavity 042, the test module 01 is arranged in the culture cavity 042, and the culture unit and the camera head are arranged oppositely.

In the embodiment, a cross mounting interlayer 043 is arranged in the box space, the mounting interlayer 043 divides the box space into two parts, namely a mounting cavity 041 for mounting a camera body and a culture cavity 042 for mounting a test module 01, and the control module 03 is accommodated and mounted by utilizing the interlayer cavity in the box space, so that the control module 03 is protected from the external environment, and the space clerk can conveniently maintain and operate.

Thus, by this layered design, the experimental load can achieve precise control of the microorganism culture environment in a limited space, while providing an optimal viewing position for the camera. This design is particularly important in space-constrained environments, such as space stations, because it allows efficient microbial cultivation and monitoring of experimental loads in a compact space.

Further, as shown in fig. 4, the outer wall surface of the side of the mounting interlayer 043 facing the culture cavity 042 forms a mounting reference surface 044, and all culture units of the test module 01 are arranged opposite to the mounting reference surface 044.

In this way, the mounting datum 044 provides a standardized mounting location for the culture units of the test module 01, ensuring that all culture units remain uniformly laid out and aligned within the space station. Specifically, all the culture units in the test module 01 are disposed opposite to the mounting reference surface 044. The layout is beneficial to realizing accurate positioning of the culture units, ensures that each culture unit can be accurately monitored by the camera, and is convenient for astronauts to operate and maintain.

As shown in fig. 4, further, the camera is fixed on the installation reference surface 044, and the installation reference surface 044 is further provided with a fixing hole communicated to the interlayer cavity, and the control end electric connector 033 is fixed in the fixing hole.

Therefore, the camera is fixed on the mounting reference surface 044, so that the camera can be ensured to be kept stable in the microgravity environment of the space station, and the growth condition of microorganisms in the culture unit can be continuously and accurately captured. Moreover, through the control end electric connector 033 in the fixed orifices, on the one hand, the electric connection between the control module 03 and the test module 01 can be realized, and on the other hand, the control module 03 can communicate with the pump body 11 and the heating element 5 in the test module 01, so that the accurate control of the culture condition is realized. In summary, the above design simplifies electrical wiring, reduces potential points of failure, while maintaining compactness and reliability of the system.

As shown in fig. 2-5, according to some embodiments of the utility model, the number of test modules 01 is not limited to one set, and similarly, the number of control side electrical connectors 033 and cameras is more than one set. That is, the number of the test modules 01 may be two or more, the number of the control-end electrical connectors 033 and the number of the cameras may be two or more, and the numbers of the test modules 01, the control-end electrical connectors 033 and the cameras are all equal and the positions are corresponding.

As shown in fig. 2-5, in one specific embodiment, the number of test modules 01, control side electrical connectors 033, and cameras are all three groups. Each set of control side electrical connectors 033 includes two separate control side electrical connectors 033.

As shown in fig. 2 to 5, the test module 01 is used for loading a microorganism sample, is a microorganism culture experimental functional area, supports on-orbit disassembly and installation of astronauts, and mainly comprises a test module 01 shell, a culture unit and a test module 01 electric connector 7. The test module 01 includes a first test module 011, a second test module 012, and a third test module 013 that are sequentially arranged along the length direction of the mounting interlayer 043, and three groups of control end electrical connectors 033 are also sequentially arranged along the length direction of the mounting interlayer 043 and respectively correspond to the three groups of test modules.

The first test module 011 is connected to two control side electrical connectors 033 together in a group. The first test module 011 includes 16 independent incubation units. The independent culture units can independently control the temperature through the control module 03, wherein the temperature control range is 25-43 ℃, and the precision is +/-1 ℃. The independent culture units can also be used for independently injecting liquid through the control module 03, the liquid adding amount range is adjustable from 0.5mL to 3.0mL, and the time-sharing liquid injection is supported. The mutually independent culture units provide nutrient supply, temperature control and humidity conditions required by the growth of microorganisms through the control of the control module 03, and supplement liquid according to experimental requirements so as to realize the dynamic control of the on-orbit experimental process of microorganisms.

The second test module 012 is connected to two control-side electrical connectors 033 together in a group. The second test module 012 includes two main parts, one of which is composed of 3 culture units having larger sizes and the other of which is composed of 8 culture units having smaller sizes, which are independent of each other. Each culture unit can independently control the temperature through the control module 03, the temperature control range is 25-43 ℃, and the precision is +/-1 ℃. Nutrient supply and temperature control for each of the culture units within the second test module 012 are similar to those of the first test module 011 and will not be described again.

The third test module 013 is connected with two control end electric connectors 033 which are a group, the first test module 011 comprises 16 mutually independent culture units and has basically the same structure as the second test module 012, and nutrient supply and temperature control of each culture unit in the third test module 013 are similar to those of the first test module 011, so that the structure and functions of the third test module 013 are not repeated here.

As shown in fig. 2 to 5, the monitoring module 02 mainly comprises a first camera 021, a second camera 022 and a third camera 023 which are sequentially arranged along the length direction of the mounting interlayer 043, wherein the camera of the first camera 021 is opposite to the first test module 011, the camera of the second camera 022 is opposite to the second test module 012, and the camera of the third camera 023 is opposite to the third test module 013. And the cameras of the first camera 021, the second camera 022 and the third camera 023 are respectively provided with a first aperture 024, a second aperture 025 and a third aperture 026.

The first camera 021 performs static imaging and dynamic imaging on the first test module 011 through the control module 03, the static imaging resolution is 2464 multiplied by 2056, the dynamic imaging frame frequency is 1fps, meanwhile, the dynamic image data is compressible, and the compression ratio is 16:1. The focal length of the lens of the first camera 021 is 5mm, the shooting object distance is not more than 100mm, and the overall view of the first test module 011 can be covered.

The second camera 022 performs static imaging and dynamic imaging on the second test module 012 under the control of the control module 03, the third camera 023 performs static imaging and dynamic imaging on the third test module 013 under the control of the control module 03, and the configuration of the second camera 022 and the third camera 023 is the same as that of the first camera 021, which is not described herein.

The first aperture 024 is controlled to illuminate the first camera 021 through the control module 03 and can adjust the illumination intensity of the microorganisms in the first test module 011, the second aperture 025 is controlled to illuminate the second camera 022 through the control module 03 and can adjust the illumination intensity of the microorganisms in the second test module 012, and the third aperture 026 is controlled to illuminate the third camera 023 through the control module 03 and can adjust the illumination intensity of the microorganisms in the third test module 013.

The following description describes various embodiments of the structure of the in-device test module 01 of the present utility model.

As shown in fig. 6 to 9, the culture unit according to the embodiment of the present utility model mainly includes three parts, namely, a culture assembly, a gas-liquid storage assembly, and a transmission assembly.

Wherein, the culture assembly comprises a sealed culture pond 1, and the culture pond 1 is used for a culture test of microorganisms. By adopting the sealed culture pond 1 design, the microbial experiment process can not pollute the external environment, and the influence of the external environment on the experiment process is prevented.

The gas-liquid storage assembly comprises a storage pool 2 and a turnover membrane 3, wherein the storage pool 2 is connected to the culture pool 1, a first accommodating cavity 4 is formed between the gas-liquid storage assembly and the culture pool 1, the transmission assembly can be installed through the first accommodating cavity 4, the structure is compact, the overall structure is miniaturized, the turnover membrane 3 is arranged in the storage pool 2, the inner cavity of the storage pool 2 is divided into a sealed liquid storage cavity 201 and a sealed gas storage cavity, and the effective isolation of the culture solution in the liquid storage cavity 201 and the gas in the gas storage cavity is realized through dividing the inner part of the storage pool 2 into two independent closed spaces.

The transmission assembly is arranged in the first accommodating cavity 4 and comprises an infusion pipeline and an air outlet pipeline, the culture pond 1 is connected with the liquid storage cavity 201 through the infusion pipeline, the infusion pipeline is used for conveying culture solution from the liquid storage cavity 201 to the culture pond 1 to activate microorganism culture, the culture solution can be distilled water, aqueous solution or nutrient solution and the like, the culture pond 1 is connected with the air storage cavity through the air outlet pipeline, and redundant gas in the culture pond 1 flows into the air storage cavity through the air outlet pipeline, so that a closed gas-liquid circulation system is formed.

Specifically, the transfer line can be with the culture solution transmission of the stock solution intracavity 201 of storage pond 2 to cultivate in pond 1, for microorganism growth provides nutrient substance, in liquid transmission, because cultivate pond 1 and storage pond 2 are seal structure, the inside pressure increase of pond 1 cavity is cultivateed to the culture solution, thereby make the gas in the pond 1 independently flow into the gas storage intracavity of storage pond 2 through the gas outlet pipe, oppression upset membrane 3 takes place deformation upset, and then the pressure in the pond 1 cavity is cultivateed in the relief, in order to guarantee the inside pressure balance of whole device, effectively improve experimental security.

It can be understood that the design of the turnover membrane 3 skillfully utilizes the liquid pressure, and when the pressure is generated by increasing the gas in the gas storage cavity, the turnover membrane 3 is pressed to deform, so that the autonomous pressure relief is realized, and the pressure balance in the whole device is maintained.

The culture unit provided by the embodiment of the utility model can effectively control the on-orbit microorganism culture experiment process by taking the culture solution in the conveying storage pool 2 as the activation condition of microorganism culture in the culture pool 1, and can automatically remove the pressure in the device by the turnover membrane 3 without additional operation and auxiliary equipment, thereby simplifying the structure of the device, being beneficial to miniaturization of the whole structure, improving the operation convenience, realizing the gas-liquid circulation balance requirement in the rigid sealing device and further ensuring the safety.

According to an embodiment of the present utility model, referring to fig. 8, the turning film 3 is provided with a protrusion 301 protruding toward the direction of the gas storage chamber, and the protrusion 301 may be a hemispherical surface having an arc surface. When the gas in the gas storage cavity presses the protruding portion 301, the protruding portion can be deformed and overturned, and the protruding portion is completely recessed to one side of the gas storage cavity 201.

According to the embodiment of the utility model, the bulge 301 of the hemispherical structure protruding towards the direction of the gas storage cavity is arranged on the turnover membrane 3, so that the pressure relief performance of the turnover membrane 3 is enhanced by the special ingenious design, and a certain amount of gas can be generated along with the growth and propagation of microorganisms in the culture pond 1 in the actual operation process. When the gas pressure increases to some extent, it acts on the protrusion 301 of the turning film 3. Because the convex part 301 adopts a hemispherical design, the convex part has good response to the pressure of gas, and can more effectively convert the received pressure into deformation kinetic energy, so that the convex part 301 can flexibly concavely overturn towards one side of the liquid storage cavity 201.

According to one embodiment of the utility model, the turning film 3 is preferably a silica gel turning film.

It can be understood that silica gel is a polymer elastomer material, has good high temperature and low temperature resistance and good chemical stability, and the silica gel turning film has excellent flexibility and elasticity, and excellent air tightness and liquid tightness.

According to one embodiment of the present utility model, as shown with reference to FIGS. 6-8, the culture assembly further comprises a heating element 5, a plurality of insulating blocks 6, a temperature sensor, and an electrical connector 7. The heating element 5 is coated on the outer wall of the culture pond 1 and used for heating the cavity of the culture pond to provide the temperature required by the growth of microorganisms, the plurality of heat insulation blocks 6 are arranged on the outer side of the heating element 5 at intervals to realize heat insulation and heat preservation, the temperature sensor is arranged in the culture pond 1 and used for detecting the temperature of the cavity of the culture pond, and the electric connector 7 is arranged in the first accommodating cavity 4 and is respectively connected with the infusion pipeline, the heating element 5 and the temperature sensor and used for receiving remote instructions and controlling the culture of microorganisms.

According to one embodiment of the present utility model, the heating member 5 is preferably a polyimide heating film, the height of the heating member 5 is 18mm, the developed length is 90mm, and the power consumption is 1W.

In the embodiment of the utility model, the polyimide material is very suitable for manufacturing heating elements in spacecrafts due to the excellent properties, such as high temperature resistance, high insulativity, high mechanical strength, good chemical stability, strong thermal stability and the like.

In the concrete parameter setting, the height of the heating element 5 is 18mm, the unfolding length is 90mm, and the dimension design can be effectively bonded and cover the outer wall of the culture pond 1, so that the heat is ensured to be uniformly distributed to the whole culture area, and the temperature condition required by the growth of microorganisms is met. The power consumption is designed to be 1W, so that the heating efficiency is ensured, the limitation of space station energy supply is considered, the purpose of low-power consumption and high-efficiency heating is realized, and the long-term stable operation and energy management of on-orbit equipment are facilitated. The precise and energy-saving design concept has important significance for experimental equipment in a space environment.

According to one embodiment of the present utility model, referring to fig. 6 to 8, a first flange 101 and a second flange 102 are provided at both ends of the culture tank 1, respectively, a second accommodating chamber 103 is formed between the first flange 101 and the second flange 102, and the heating element 5 and the heat insulating block 6 are located in the second accommodating chamber 103.

In this embodiment, a first flange 101 and a second flange 102 are specially designed at two ends of the top and bottom of the culture pond 1, and after the two flanges are oppositely arranged, an independent second accommodating cavity 103 is formed. The heating element 5 and the heat insulating block 6 are neatly arranged in this second housing chamber 103 in such a layout that the heating element and the main body portion of the culture tank form a nested space utilization structure.

Through this design, on the one hand, can ensure that heating element 5 hugs closely and effectively heats cultivate pond 1, on the other hand, thermal-insulated piece 6 can prevent effectively that the heat from dispelling to the device outside, does not occupy the inside space of cultivate pond 1 again simultaneously to the validity and the stability of microorganism cultivation environment have been guaranteed. In addition, the heating component (the heating piece 5 and the heat insulation block 6) is integrated in the second accommodating cavity 103 between the turnups at the end parts of the culture pond 1, so that the integration of the functional modules is realized, space resources are greatly saved, the miniaturization and the light weight of the whole microorganism test module are facilitated, and the requirements of strict limitation on the volume and the weight of equipment in the space environment are met.

According to one embodiment of the present utility model, as shown with reference to FIGS. 6-8, the incubation assembly further comprises a sample holder 8, a compression frame 9, and a viewing window 10. The sample bracket 8 is detachably arranged in the culture pond 1, a fixing hole is formed in the sample bracket 8 and used for fixing a tested material sheet on the sample bracket 8 through two fixing screws, the fixing screws are M2 screws with gaskets and washers, the tested material sheet is a metal or nonmetal material sheet, the surface of the tested material sheet is inoculated with tested microorganisms, the sample bracket 8 is provided with an overflow gap 801 and is convenient for gas and liquid circulation in the culture pond 1, the compression frame 9 is detachably connected to a first flange 101 at the top end of the culture pond 1, and the window 10 is arranged between the first flange 101 at the top end of the culture pond 1 and the compression frame 9. Wherein the microorganism to be tested may be Aspergillus, penicillium, cladosporium, fusarium, staphylococcus, corynebacterium, bacillus, micrococcus and/or Pseudomonas.

In the design of the culture component of the embodiment of the utility model, key components such as a sample bracket 8, a pressing frame 9, a window 10 and the like are further introduced.

Wherein, sample bracket 8 detachably installs in the inside of cultivateing pond 1, has preset the fixed orifices on it for firmly bear the test material piece. The sample bracket 8 is also specially designed with an overflow gap 801, so that the free circulation of gas and liquid in the culture pond 1 is ensured, and the full contact of microorganisms and nutrient solution and the metabolic activity are facilitated.

The pressing frame 9 is also detachably designed and is connected to the first flanging 101 at the top end of the culture pond 1. The function of the pressing frame 9 is to firmly position the window 10 and to play a sealing role, ensure the integrity of the culture environment and prevent liquid leakage or gas escape.

The window 10 is arranged at the position between the first flanging 101 at the top end of the culture pond 1 and the pressing frame 9, so that scientific researchers can observe the growth condition of microorganisms on the sample bracket 8 and the change of the whole culture process through the window 10 without opening the device.

According to one embodiment of the present utility model, window 10 is preferably a polycarbonate window.

It is understood that polycarbonate has excellent light transmittance, extremely high impact strength, relatively low density and good weather resistance, and is easily processed into various shapes and sizes, meets the requirement of complex space structure, and can improve wear resistance and scratch resistance through surface treatment.

Therefore, the embodiment of the utility model not only meets the visual requirement in the microorganism culture experiment process, but also fully considers the strict requirement on materials in the space environment by selecting the polycarbonate as the window material.

According to one embodiment of the utility model, the culture pond 1 and the compression frame 9 are preferably made of aluminum alloy materials, so that the quality of the whole test module can be remarkably reduced, and the requirements of a spacecraft on strict control of the weight of a payload are met.

In a specific example, screw holes are respectively formed at the four top corners of the first flanging 101, the pressing frame 9 and the window 10 at the top end of the culture pond 1, and fastening assembly is performed through four M2 screws. And in the actual process, a sealing gasket can be arranged at the joint to ensure the tightness of the culture pond 1.

According to one embodiment of the utility model, referring to FIG. 9, a first support column 104 and a second support column 105 are arranged in the culture pond 1 at intervals, the first support column 104 is provided with a connecting hole 1041, the second support column 105 is provided with a liquid inlet channel 1051, the sample bracket 8 is arranged on the first support column 104 and the second support column 105, the sample bracket 8 is connected with the connecting hole 1041 of the first support column 104 through a fastener, a liquid conveying pipeline is communicated with a flowing-through gap 801 of the sample bracket 8 through the liquid inlet channel 1051 of the second support column 105, an air outlet hole 106 is arranged at the bottom of the culture pond 1, and the air outlet hole 106 is connected with the air outlet pipeline.

For example, two first support columns 104 and one second support column 105 are located on opposite sides of the interior of the culture pond 1, respectively, which is advantageous in improving the stability of the sample carrier 8.

Further, the first support column 104 is provided with a connection hole 1041 for fixing the sample holder 8. The sample bracket 8 is tightly connected with the first support column 104 through fasteners such as screws, so that the sample bracket 8 and the tested material sheet are ensured to be stable and motionless in the culture process, and movement or overturning caused by the influence of microgravity environment is avoided.

Further, the second support column 105 is provided with a liquid inlet channel 1051 in addition to the supporting function, which is designed to directly communicate the liquid transfer tube with the flow gap 801 on the sample carrier 8. The culture solution can be directly conveyed to the sample bracket 8 from the liquid inlet channel 1051, so that microorganisms can be quickly contacted with fresh culture solution after being inoculated on the surface of a tested material sheet, and the culture efficiency and the possibility of maintaining the activity of the microorganisms are improved.

According to one embodiment of the present utility model, as shown in FIGS. 6-8, the infusion line basically comprises a fluid inlet tube, a pump body 11, a thermal insulation cushion 12 and the like. The liquid inlet pipe is respectively connected with liquid storage cavities 201 of the culture pond 1 and the storage pond 2, the pump body 11 is clamped in the first accommodating cavity 4 between the culture pond 1 and the storage pond 2, the pump body 11 is positioned in the liquid inlet pipe, and the heat insulation buffer cushion 12 is arranged between the pump body 11 and the storage pond 2, namely positioned at the bottom of the pump body 11.

Therefore, the design of the infusion pipeline in the embodiment realizes the organic combination of compact structure, miniaturization and functionality, and meets the requirements of the on-orbit microorganism test module on high efficiency, accuracy and safety.

According to one embodiment of the utility model, the inlet and outlet lines are latex or silicone tubes, and the pump body 11 is preferably a peristaltic pump.

Therefore, the latex tube or the silica gel tube is matched with the peristaltic pump to serve as the liquid inlet and outlet component in the microorganism test module, so that high-efficiency and accurate liquid transmission control is realized, and special requirements under on-orbit experiment conditions are fully considered.

The flow rate of the pump body 11 was set to 3ml/min, the accuracy was 0.1ml, and the control of the pump body 11 was achieved by the electrical connector 7.

According to one embodiment of the utility model, referring to FIG. 8, the infusion tube further comprises a wire bundle seat 13, wherein the wire bundle seat 13 is clamped on the side surface of the first accommodating cavity 4 between the second flanging 102 at the bottom end of the culture pond 1 and the heat insulation buffer pad 12, a plurality of first wire bundle holes 131 and second wire bundle holes 132 are formed in the wire bundle seat 13, the first wire bundle holes 131 are used for penetrating and fixing a liquid inlet tube, and the second wire bundle holes 132 are used for penetrating and fixing wires of the temperature sensor, the electric connector 7, the pump body 11 and other parts.

Thus, the design of the wire harness seat 13 in the embodiment of the utility model fully embodies engineering optimization thought under space environment conditions with limited space, not only meets the functional requirements, but also considers the light weight and compactness principles required by spacecraft application.

According to an embodiment of the present utility model, referring to fig. 6 to 8, the left and right sides of the heat insulation cushion pad 12 are respectively provided with a fixing ring 121, and the inlet pipe and the outlet pipe are respectively and correspondingly arranged through the fixing rings 121.

Specifically, a first connecting pipe 107 and a second connecting pipe 108 are respectively arranged at the left side and the right side of the bottom of the culture pond 1, the first connecting pipe 107 is communicated with a liquid inlet channel 1051 of a second supporting column 105 in the culture pond 1, the second connecting pipe 108 is communicated with an air outlet hole 106 at the bottom of the culture pond 1, a third connecting pipe 202 communicated with a liquid storage cavity 201 and a fourth connecting pipe 203 communicated with a gas storage cavity are arranged on the storage pond 2, the first connecting pipe 107, a left fixing ring 121 and the third connecting pipe 202 are correspondingly arranged along the height direction, and the second connecting pipe 108, the right fixing ring 121 and the fourth connecting pipe 203 are correspondingly arranged along the height direction.

One end of the liquid inlet pipe is connected with the first connecting pipe 107 at the bottom of the culture pond 1, the other end of the liquid inlet pipe is connected with the third connecting pipe 202 of the liquid storage cavity 201 after penetrating through the wire bundle seat 13 and the left fixing ring 121, one end of the air outlet pipe is connected with the second connecting pipe 108 at the bottom of the culture pond 1, and the other end of the air outlet pipe is connected with the fourth connecting pipe 203 of the air storage cavity after penetrating through the right fixing ring 121.

According to an embodiment of the present utility model, referring to fig. 6 to 8, a connection frame 14 is provided between the heat insulation buffer pad 12 and the storage tank 2, the connection frame 14 is detachably connected with the storage tank 2, and a plurality of connection columns 109 are provided at intervals at the bottom edge of the culture tank 1, and the connection columns 109 are detachably connected with the connection frame 14 through the heat insulation buffer pad 12, so that a first accommodating chamber 4 can be formed between the culture tank 1 and the heat insulation buffer pad 12.

For example, the four corners of the cushion pad 12 are provided with the first connecting portions 122, the four corners of the connecting frame 14 are provided with the second connecting portions 141, the four corners of the cover 205 of the storage pool 2 are provided with the third connecting portions 206, and the bottom four corners of the culture pool 1 are provided with the connecting posts 109, respectively. During assembly, the second connecting portion 141 of the connecting frame 14 is fastened to the third connecting portion 206 of the lid 205 of the storage pool 2 by screws, and the second connecting portion 141 of the connecting frame 14 and the first connecting portion 122 of the heat insulating cushion pad 12 are simultaneously connected to the connecting column 109 at the bottom of the culture pool 1 by screws penetrating from bottom to top.

Therefore, the structural design fully considers the special requirements in the space environment, ensures flexible and convenient assembly among all components, and also meets the requirements of heat management and vibration reduction, thereby having significant significance for improving the overall performance of the on-orbit microorganism experimental equipment.

According to one embodiment of the utility model, the connecting frame 14 is provided with a number of lightening holes 142 to reduce the overall mass.

According to one embodiment of the utility model, the volume of the storage pool 2 can be 3ml, the storage pool 2 comprises a pool body 204 and a cover body 205 which are detachably connected through four screws, a turnover membrane 3 is arranged between the pool body 204 and the cover body 205, a liquid storage cavity 201 is formed between the pool body 204 and the turnover membrane 3, and a gas storage cavity is formed between the cover body 205 and the turnover membrane 3.

The tank 204 is provided with a third connection pipe 202 communicating with the liquid storage chamber 201, and the cover 205 is provided with a fourth connection pipe 203 communicating with the liquid storage chamber.

According to one embodiment of the utility model, the cavity of the culture pond 1 is a cylindrical cavity with the inner diameter of 30mm and the depth of 20mm, the material is aluminum alloy, a space is provided for microorganism culture, the space volume is about 14ml, a proper amount of microorganism culture solution and a tested material sheet can be contained, and the requirement of a specific microorganism experiment on the culture environment capacity is met. Meanwhile, the compact design is also beneficial to miniaturization and integration of the whole microorganism test module.

According to one embodiment of the present utility model, referring to fig. 8, the number of the heat insulation blocks 6 is four, and the heat insulation blocks 6 are respectively arranged at the four top corners of the culture pond 1, and each heat insulation block 6 is in the shape of an isosceles trapezoid, and the inner side of each heat insulation block is an arc surface matched with the cylindrical culture pond 1, so that the outer side surface of each heat insulation block 6 is tangent to the outer wall of the cylindrical culture pond 1 to form a square body.

By means of the shape design, the heat insulation block 6 can be tightly attached to the outer wall of the cylindrical culture pond 1, good contact area is ensured, the influence of the external environment on the temperature in the culture pond can be effectively prevented, heat loss is reduced, and meanwhile potential safety risks caused to external equipment or astronauts by high temperature in the culture pond 1 are prevented.

According to one embodiment of the present utility model, referring to fig. 6, the culture assembly, the transmission assembly and the gas-liquid storage assembly of the microorganism test module of the present utility model are stacked in the height direction, and the overall device is a rectangular parallelepiped with a shape of approximately 60mm (length) 60mm (width) 900mm (height), that is, a volume of only 324cm ³, so that a parallel test can be performed on a plurality of devices in a limited space at the same time.

The working principle of the culture unit provided by the utility model is described below with reference to specific examples, and the culture unit generally comprises:

1. And controlling the process of the microorganism culture experiment, wherein before the microorganism culture experiment is started, the inoculated microorganism is dry spores of the microorganism, and the microorganism is in a dormant state and does not grow and reproduce. When the culture needs to be started, the transportation culture solution is used as an activation condition, the temperature control function is started at the same time, and after the microorganism has a growth condition, the microorganism starts to grow and reproduce.

2. In the liquid conveying working process, the liquid is conveyed by a micro peristaltic pump, after the micro peristaltic pump is started, the culture solution flows out of the liquid storage cavity 201 of the storage tank 2 through a liquid inlet pipe and the micro peristaltic pump and finally reaches the cavity of the culture tank 1, so that nutrient substances are provided for the growth of microorganisms. When the liquid is conveyed, as the culture pond 1 and the storage pond 2 are of sealing structures, the pressure in the cavity of the culture pond 1 can be increased, and the gas in the cavity of the culture pond 1 can automatically flow into the gas storage cavity of the storage pond 2 through the gas outlet pipeline to press the silica gel overturning film to deform and overturn, so that the pressure in the cavity of the culture pond 1 is removed, and the balance of the pressure in the whole device is ensured. After the test, the storage pool 2 is disassembled, and the silica gel turning film can be observed to be completely sunken to one side of the liquid storage cavity 201.

In conclusion, the on-orbit microorganism culture experimental load has the following beneficial effects:

(1) The experimental load has high system integration and high flux characteristics, and can be used for simultaneously loading 3 mutually independent experimental modules 01, and each experimental module 01 can be used for loading 16 mutually independent culture units. Each culture unit can realize independent temperature control, the temperature control range is 25-43 ℃, and the precision is +/-1 ℃. Each culture unit can realize independent liquid injection, and the liquid adding amount is adjustable by 0.5-3.0mL, so that time-sharing liquid injection is supported. Therefore, 48 on-orbit experiments of independent temperature control and independent liquid injection can be simultaneously carried out.

(2) The experimental load has the dynamic monitoring function of the on-orbit working state of the load and the on-orbit experiment. The on-orbit working state, the on-orbit experiment process and the on-orbit experiment result of the load can be fed back in time through the remote measurement parameters, the engineering parameters and the image data, so that an experimenter is guided to set the state of the experiment load and dynamically adjust the experiment process. Experimental load imaging includes static imaging and dynamic imaging, the static imaging resolution is 2464×2056, the dynamic imaging frame frequency is 1fps, and meanwhile, the dynamic image data is compressible, and the compression ratio is 16:1.

(3) The experimental load breaks through the technical difficulties of nutrient supply, temperature control, humidity condition and dynamic control of experimental progress required by the microorganism culture experiment under the microgravity condition. The test module 01 is loaded with a microorganism sample, provides nutrient supply, temperature control and humidity conditions required by microorganism growth, supplements liquid according to the experiment requirement, and realizes dynamic control of microorganism on-orbit experiment progress.

(4) The experimental load of the utility model has high safety. The experimental module 01 of the experimental load adopts a double-sealing design, the inner layer meets the requirement of liquid tightness, the outer layer meets the requirement of air tightness, and a space biological experiment with the biological safety grade of BSL2 can be carried out. The experimental load is designed with a high-temperature sterilization function, and the culture unit can be heated to 100 ℃. On one hand, the method can kill microorganisms in an emergency when the risk of the microorganism experiment is found, ensure the safety of the experiment process, and on the other hand, the method can kill microorganisms when the experiment waste is treated, and ensure the safety of the waste.

(5) The experimental load of the utility model has high universality. The experimental load adopts a modularized design, and the experimental module 01 can be detached and installed on the track and meets the uplink and downlink requirements. The electric control module provides a standardized telecommunication interface, is provided with a 48-path pump control interface, a 48-path temperature control interface and a 48-path temperature acquisition interface, and can be used for modifying the test module 01 according to experimental requirements.

(6) The experimental load of the utility model has high reliability. The experimental load accords with the telecommunication interface of the China space station standard, and the structural design meets the mechanical properties of the China space station load uplink and load downlink environments. The electromagnetic compatibility design of the experimental load accords with the manned aerospace engineering standard and the space station standard.

(7) The experimental load meets the man-machine efficiency requirement, and has the advantages of cheapness in operation and interface safety of astronauts.

It should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present utility model, and not for limiting the same, and although the present utility model has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that the technical solution described in the above-mentioned embodiments may be modified or some technical features may be equivalently replaced, and these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solution of the embodiments of the present utility model.

Claims

1. An in-orbit microbial culture experimental load, comprising:

The load box body is internally provided with a box body space;

2. The on-orbit microorganism culture experiment load according to claim 1, wherein a heating element is further arranged on the outer side of the culture pond, and a pump body is arranged on the infusion pipeline;

3. The on-orbit microbiological culture experimental load according to claim 2, wherein the monitoring module comprises a plurality of cameras and a plurality of diaphragms, one diaphragm is arranged on each camera, and the cameras and the diaphragms are connected with the control module.

4. An in-orbit microbiological culture experiment load according to claim 3, wherein a mounting interlayer is mounted in the load housing across the housing space, the mounting interlayer dividing the housing space into a mounting cavity and a culture cavity;

5. The on-orbit microorganism culture experiment load according to claim 4, wherein an outer wall surface of one side of the installation interlayer facing the culture cavity forms an installation reference surface, the camera is fixed on the installation reference surface, and all culture units of the test module are arranged opposite to the installation reference surface.

6. The on-orbit microorganism culture experiment load according to claim 5, wherein the control module is installed in an interlayer cavity inside the installation interlayer, a fixing hole communicated to the interlayer cavity is further formed in the installation reference surface, and the control module comprises a control end electric connector for realizing electric connection, and the control end electric connector is fixed in the fixing hole.

7. The on-orbit microorganism culture experimental load according to claim 4, wherein the test module comprises a first test module, a second test module and a third test module, the monitoring module comprises a first camera, a second camera and a third camera, and the first camera, the second camera and the third camera are respectively provided with a first aperture, a second aperture and a third aperture;

8. The on-orbit microbial cultivation experiment load according to any one of claims 2 to 7, wherein the control module comprises an interconnected power distribution computer processing circuit board, a drive control circuit board and a control side electrical connector.

9. The on-orbit microbiological culture test load according to claim 8, wherein the power distribution computer processing circuit board comprises a power distribution unit and a computer processing unit;

10. The on-orbit microbial cultivation experiment load according to claim 9, wherein the drive control circuit board comprises:

A filter circuit;

The pump body driving circuit is connected with the FPGA circuit;

And the heating element control circuit is connected with the FPGA circuit.